Predicted mutation
evidence seq id position mutation annotation gene description
RA USA300TCH1516_ALE 2,372,863 C→G G305R (GGA→CGA)  pbuG ← Guanine/hypoxanthine permease PbuG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,372,8630CG97.5% 423.0 / 0.3 121G305R (GGA→CGA) pbuGGuanine/hypoxanthine permease PbuG
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base G (62/56);  total (64/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

Only 100 of 121 total aligned reads displayed.

ATTAACGGACTAAAGAACAGCGCTAATAGGAAACAACAACCTGTTACGATACTAGCAAATCCTGTTCTAGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTAGCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAA  >  USA300TCH1516_ALE/2372727‑2373001
                                                                                                                                        |                                                                                                                                          
aTTAACGGACTAAAGTACATCGCTAATAGGAAACAACAACCTGTTACGATACTAGCAAATCCTGTTCTAGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCGTACa                                                                                                                                        <  2:79489/141‑1 (MQ=255)
aTTAACGGACTAAAGAACAGCGCTAATAGGAAACAACAACCTGTTACGATACTAGCAAATCCTGTTCTAGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCGTACa                                                                                                                                        >  1:280372/1‑141 (MQ=255)
  tAACGGACTAAAGAACAGCGCTAATAGGAAACAACAACCTGTTACGATACTAGCAAATCCTGTTCTAGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCGTACAAt                                                                                                                                      <  2:606000/141‑1 (MQ=255)
  tAACGGACTAAAGAACAGCGCTAATAGGAAACAACAACCTGTTACGATACTAGCAAATCCTGTTCTAGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTACAAAAACTGATCGTACAAt                                                                                                                                      <  2:386437/141‑1 (MQ=255)
        aCTAAAGAACAGCGCTAATAGGAAACAACAACCTGTTACGATACTAGCAAATCCTGTTCTAGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGc                                                                                                                                <  1:697287/141‑1 (MQ=255)
         cTAAAGAACAGCGCTAATAGGAAACAACAACCTGTTACGATACTAGCAAATCCTGTTCTAGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCt                                                                                                                               >  2:574976/1‑141 (MQ=255)
          tAAAGAACAGCGCTAATAGGAAACAACAACCTGTTACGATACTAGCAAATCCTGTTCTAGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTa                                                                                                                              <  2:275864/141‑1 (MQ=255)
           aaaGAACAGCGCTAATAGGAAACAACAACCTGTTACGATACTAGCAAATCCTGTTCTAGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTaa                                                                                                                             <  3:232905/141‑1 (MQ=255)
           aaaGAACAGCGCTAATAGGAAACAACAACCTGTTACGATACTAGCAAATCCTGTTCTAGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTaa                                                                                                                             <  3:67930/141‑1 (MQ=255)
            aaGAACAGCGCTAATAGGAAACAACAACCTGTTACGATACTAGCAAATCCTGTCCTAGCACCTACATCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCCTACAATCGTAGCTaaa                                                                                                                            <  1:347013/141‑1 (MQ=255)
                aCAGCGCTAATAGGAAACAACAACCTGTTACGATACTAGCAAATCCTGTTCTAGCACCTACAGCAACACCTGAGGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAAt                                                                                                                        >  1:737493/1‑141 (MQ=255)
                 cAGCGCTAATAGGAAACAACAACCTGTTACGATACTAGCAAATCCTGTTCTAGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAATc                                                                                                                       >  4:143657/1‑141 (MQ=255)
                  aGCGCTAATAGGAAACAACAACCTGTTACGATACTAGCAAATCCTGTTCTAGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGAAAAAAAACCAAAAACTGATCGTACAATCGTAGCTAAAGAATcc                                                                                                                      <  2:666879/141‑2 (MQ=255)
                   gcgcTAATAGGAAACAACAACCTGTTACGATACTAGCAAATCCTGTTCTAGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCTAAAACTGATCGTACAATCGTAGCTAAAGAATCTg                                                                                                                     >  2:286080/1‑141 (MQ=255)
                   gcgcTAATAGGAAACAACAACCTGTTACGATACTAGCAAATCCTGTTCTAGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTg                                                                                                                     >  1:128670/1‑141 (MQ=255)
                     gcTAATAGGAAACAACAACCTGTTACGATACTAGCAAATCCTGTTCTAGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTgag                                                                                                                   >  2:735095/1‑141 (MQ=255)
                     gcTAATAGGAAACAACAACCTGTTACGATACTAGCAAATCCTGTTCTAGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTgag                                                                                                                   >  1:805309/1‑141 (MQ=255)
                         aTAGTAAACAACAACCTGTTACGATACTAGCAAATCCTGTTCTAGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTAAAAAAGAAAAAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAGAACa                                                                                                               <  2:796715/141‑1 (MQ=255)
                           aGGAAACAACAACCTGTTACGATACTAGCACATCCTGTTCTAGCACCTACAGCAACCCCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCCAAAGAATCTGGGAACAAt                                                                                                             >  4:127755/1‑141 (MQ=255)
                           aGGAAACAACAACCTGTTACGATACTAGCAAATCCTGTTCTAGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTaaa                                                                                                                            <  1:357234/126‑1 (MQ=255)
                           aGGAAACAACAACCTGTTACGATACTAGCAAATCCTGTTCTAGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTaaa                                                                                                                            >  2:357234/1‑126 (MQ=255)
                           aGGAAACAACAACCTGTTACGATACTAGCAAATCCTGTTCTAGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAAt                                                                                                             >  1:178139/1‑141 (MQ=255)
                            ggAAACAACAACCTGTTACGATACTAGCAAATCCTGTTCTAGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATg                                                                                                            >  1:858130/1‑141 (MQ=255)
                                   acaacCTGTTACGATACTAGCAAATCCTGTTCTAGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTAcc                                                                                                     >  3:357919/1‑141 (MQ=255)
                                   acaacCTGTTACGATACTAGCAAATCCTGTTCTAGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTAcc                                                                                                     <  1:8925/141‑1 (MQ=255)
                                   acaacCTGTTACGATACTAGCAAATCCTGTTCTAGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTAGTTGTAGTTGTTCCAACAACTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTAcc                                                                                                     >  1:138195/1‑141 (MQ=255)
                                    caacCTGTTACGATACTAGCAAATCCTGTTCTAGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTATCTAAAGAATCTGAGAACAATGCTCTACCa                                                                                                    <  2:781407/141‑1 (MQ=255)
                                     atccTGTTACGATACTAGCAAATCCTGTTCTCGCACCTACAGCAACACCTGAAGTTGATCCGATATAAGAACTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAg                                                                                                   <  3:183842/139‑1 (MQ=255)
                                      acCTGTTACGATACTAGCAAATCCTGTTCTAGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGc                                                                                                  >  1:437421/1‑141 (MQ=255)
                                       ccTGTTACGATACTAGCAAATCCTGTTCTAGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGct                                                                                                 >  1:117402/1‑141 (MQ=255)
                                          gTTACGATACTAGCAAATCCTGTTCTAGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCtt                                                                                              <  1:606221/141‑1 (MQ=255)
                                            tACGATACTAGCAAATCCTGTTCTAGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTgg                                                                                            <  2:706445/141‑1 (MQ=255)
                                                 tACTAGCAAATCCTGTTCTAGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAAtt                                                                                       <  2:319856/141‑1 (MQ=255)
                                                 tACTAGCAAATCCTGTTCTAGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAAtt                                                                                       <  1:144308/141‑1 (MQ=255)
                                                  aCTAGCAAATCCTGTTCTAGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAAttt                                                                                      <  1:625430/141‑1 (MQ=255)
                                                      gCAAATCCTGTTCTAGCACCTACAGCAACACCTGAAGTTGATTCGATCTAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAACGAATCCGAGAACAATGCTCTACCAGCTCTTGGTAATttatta                                                                                  >  2:345938/1‑141 (MQ=255)
                                                        aaaTCCTGTTCTAGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATc                                                                                >  1:266135/1‑141 (MQ=255)
                                                        aaaTCCTGTTCTAGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAAAACAATGCTCTACCAGCTCTTGGTAATTTATTATc                                                                                <  1:304616/141‑1 (MQ=255)
                                                          aTCCTGTTCTAGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCtt                                                                              <  3:105147/141‑1 (MQ=255)
                                                             cTGTTCTAGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACGAGCTCTTGGTAATTTATTATCTTTCa                                                                           >  2:688536/1‑141 (MQ=255)
                                                             cTGTTCTAGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCa                                                                           >  3:327763/1‑141 (MQ=255)
                                                             cTGTTCTAGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTGGTTGTAGTGGCTCCAAAAACTGTACGTACAATCGTGGCGAAAGAATCTTATAACAATGCTCTACCAGCTCTTGGTAATCTATTATCTTTCa                                                                           >  3:127383/1‑141 (MQ=255)
                                                              tGTTCTAGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCat                                                                          <  3:264645/141‑1 (MQ=255)
                                                               gTTCTAGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCata                                                                         >  3:95149/1‑141 (MQ=255)
                                                               gTTCTAGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCata                                                                         >  1:626962/1‑141 (MQ=255)
                                                                ttCTAGCACCTTCTTCATCACCTTAACTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCGTACAACCGTAGCTAAAGAATCTGAGAACAATGCTCTCCCAGCTCTTGTTAATTTATTATCTGTCataa                                                                        <  2:821410/141‑1 (MQ=255)
                                                                ttCTAGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCataa                                                                        >  1:820095/1‑141 (MQ=255)
                                                                 tCTAGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCataat                                                                       >  3:118607/1‑141 (MQ=255)
                                                                  cTAGCACCTACAGCAACACCTGGAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCataata                                                                      <  2:56830/141‑1 (MQ=255)
                                                                  cTAGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCataata                                                                      <  2:722182/141‑1 (MQ=255)
                                                                   tAGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATAc                                                                     <  1:467201/141‑1 (MQ=255)
                                                                   tAGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATAc                                                                     <  1:371903/141‑1 (MQ=255)
                                                                   tAGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATAc                                                                     <  3:198255/141‑1 (MQ=255)
                                                                    aGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATAcc                                                                    <  1:446448/141‑1 (MQ=255)
                                                                    aGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATAcc                                                                    >  3:148018/1‑141 (MQ=255)
                                                                       aCCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGc                                                                 <  4:214932/141‑1 (MQ=255)
                                                                       aCCTACAGCAACACCTGAAGTCGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGc                                                                 >  4:154540/1‑141 (MQ=255)
                                                                         cTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCtt                                                               <  2:301023/141‑1 (MQ=255)
                                                                         cTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCtt                                                               <  1:735095/141‑1 (MQ=255)
                                                                            cAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAg                                                            <  1:165752/141‑1 (MQ=255)
                                                                              gCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTATTCCAAAAACTGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTa                                                          >  1:750532/1‑141 (MQ=255)
                                                                                aaCACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTAGc                                                        <  3:151187/141‑1 (MQ=255)
                                                                                aaCACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTAGc                                                        <  1:295635/141‑1 (MQ=255)
                                                                                   acCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTAGctgt                                                     <  2:273613/141‑4 (MQ=255)
                                                                                    ccTGAAGTTGATTCGATATAAGAAGTGGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTAGCAACa                                                    >  2:441820/1‑141 (MQ=255)
                                                                                    ccTGAAGTTGAGTCGACATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCGACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTAGCAACa                                                    <  2:347098/141‑1 (MQ=255)
                                                                                      tGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTCTCATAATACCTGCTTGAGTAGCAACAGc                                                  <  2:390234/141‑1 (MQ=255)
                                                                                       gAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTAGCAACAGct                                                 >  4:266211/1‑141 (MQ=255)
                                                                                        aaGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTAGCAACAGcta                                                >  2:269832/1‑141 (MQ=255)
                                                                                           ttGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTAGCAACAGctacta                                             <  3:160553/141‑1 (MQ=255)
                                                                                             gATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTAGCAACAGCTACTAAt                                           <  2:488915/141‑1 (MQ=255)
                                                                                                 cGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAg                                                            <  2:420970/120‑1 (MQ=255)
                                                                                                 cGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTCAGTAGCAACAGCTACTAATGTAc                                       >  4:254944/1‑141 (MQ=255)
                                                                                                     ataAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGACTCTGAGAACAATGATCTACCAGCTCTTAGTAATTTATTCTCTAACATAATACCTGCTTGAGTAGCAACAGCTACTAATGTACCAGc                                   <  1:393243/141‑1 (MQ=255)
                                                                                                        agaagTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTAGCAACAGCTACTAATGTACCAGCTGt                                <  2:707146/141‑1 (MQ=255)
                                                                                                        agaagTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTAGCAACAGCTACTAATGTACCAGCTGt                                >  1:2371/1‑141 (MQ=255)
                                                                                                        agaagTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTAGCAACAGCTACTAATGTACCAGCTGt                                <  3:286505/141‑1 (MQ=255)
                                                                                                          aagTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTAGCAACAGCTACTAATGTACCAGCTGTAt                              <  4:95149/141‑1 (MQ=255)
                                                                                                           agTTGTTGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTAGCAACAGCTACTAATGTACCAGCTGTATc                             >  3:98374/1‑141 (MQ=255)
                                                                                                            gttgttGTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTAGCAACAGCTACTAATGTACCAGCTGTATCa                            >  2:100055/1‑141 (MQ=255)
                                                                                                             ttgttgTAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTAGCAACAGCTACTAATGTACCAGCTGTATCaa                           >  2:514083/1‑141 (MQ=255)
                                                                                                               gttgtAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTAGCAACAGCTACTAATGTACCAGCTGTATCAAAg                         >  1:565700/1‑141 (MQ=255)
                                                                                                                ttgtAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTAGCAACAGCTACTAATGTACCAGCTGTATCAAAGa                        >  3:252105/1‑141 (MQ=255)
                                                                                                                ttgtAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTAGCAACAGCTACTAATGTACCAGCTGTATCAAAGa                        >  3:296850/1‑141 (MQ=255)
                                                                                                                ttgtAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTAGCAACAGCTACTAATGTACCAGCTGTATCAAAGa                        >  2:610900/1‑141 (MQ=255)
                                                                                                                 tgtAGTTGTTCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTAGCAACAGCTACTAATGTACCAGCTGTATCAAAGaa                       <  1:482337/141‑1 (MQ=255)
                                                                                                                   tAGTTGTTCCAACACTACATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTAGCAACAGCTACTAATGTACCAGCTGTATCAAAGAAAt                     <  1:701551/141‑1 (MQ=255)
                                                                                                                        gttCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTAGCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCaata                >  1:147571/1‑141 (MQ=255)
                                                                                                                         ttCCAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTAGCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCaagaa               >  4:319527/1‑141 (MQ=255)
                                                                                                                           ccAAAAACTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTACCTTTCATAATACCTGCTTGAGTAGCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCaataaat             <  3:126853/141‑1 (MQ=255)
                                                                                                                             aaaaaCTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTAGCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAataaataa           >  1:35919/1‑141 (MQ=255)
                                                                                                                              aaaaCTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTAGCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAg          >  1:466356/1‑141 (MQ=255)
                                                                                                                               aaaCTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTAGCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGa         >  1:163691/1‑141 (MQ=255)
                                                                                                                               aaaCTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTAGCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGa         >  2:467081/1‑141 (MQ=255)
                                                                                                                               aaaCTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTAGCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGa         >  2:634559/1‑141 (MQ=255)
                                                                                                                                 aCTGATCGTACAAACGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTAGCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAAc       >  2:545449/1‑141 (MQ=255)
                                                                                                                                  cTGATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTAGCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACg      <  1:331279/141‑1 (MQ=255)
                                                                                                                                    gATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTAGCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGtt    <  1:235627/141‑1 (MQ=255)
                                                                                                                                    gATCGTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTAGCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGtt    >  4:305875/1‑141 (MQ=255)
                                                                                                                                      tCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTAGCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTaa  >  3:44685/1‑141 (MQ=255)
                                                                                                                                        |                                                                                                                                          
ATTAACGGACTAAAGAACAGCGCTAATAGGAAACAACAACCTGTTACGATACTAGCAAATCCTGTTCTAGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCCTACAATCGTAGCTAAAGAATCTGAGAACAATGCTCTACCAGCTCTTGGTAATTTATTATCTTTCATAATACCTGCTTGAGTAGCAACAGCTACTAATGTACCAGCTGTATCAAAGAAATCAATAAATAAGAACGTTAA  >  USA300TCH1516_ALE/2372727‑2373001

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: