BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	2,709,297	T→A	K544* (AAA→TAA)	oatA_2 ←	O‑acetyltransferase OatA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,709,297	0	T	A	95.3%	360.2 / 7.4	106	K544* (AAA→TAA)	oatA_2	O‑acetyltransferase OatA
Reads supporting (aligned to +/- strand): ref base T (1/4); new base A (48/53); total (49/57)
Fisher's exact test for biased strand distribution p-value = 3.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.36e-01

Only 100 of 112 total aligned reads displayed.

ATCAGTCAGCGCTTTACTACCTGCATATTCTAAGTGAATACCGTCATATGCAAAGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTTATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAATTGATCTTTCGTAAATGCCCCATTTGTACCAAGCTCTACTACAACTT  >  USA300TCH1516_ALE/2709160‑2709431

atcaGTCAGCGCTTTACTACCTGCATATTCTAAGTGAATACCGTCATATGCAAAGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAaattaattaatt                                                                                                                                     <  1:646355/141‑1 (MQ=255)

   agtcagCGCTTTACTACCTGCATATTCTAAGTGAATACCGTCATATGCAAAGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATtaatttat                                                                                                                                  >  1:8595/1‑141 (MQ=255)

    gtcagCGCTTTACTACCTGCATATTCTAAGTGAATACCGTCATATGCAAAGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTaatttatt                                                                                                                                 <  2:592496/141‑1 (MQ=255)

     tcagCGCTTTACTACCTGCATATTCTAAGTGAATACCGTCATATGCAAAGTATTCCGGATGACCTGCAGAAGCTTTCTACCCATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATtaatttatga                                                                                                                                <  2:82672/141‑3 (MQ=255)

     tcagCGCTTTACTACCTGCATATTCTAAGTGAATACCGTCATATGCAAAGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTc                                                                                                                                >  1:440430/1‑141 (MQ=255)

        gcgcTTTACTACCTGCATATTCTAAGTGAATACCGTCATATGCAAAGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTAc                                                                                                                             >  1:199895/1‑141 (MQ=255)

         cgcTTTACTACCTGCATATTCTAAGTGAATACCGTCATATGCAAAGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTAcc                                                                                                                            >  2:679940/1‑141 (MQ=255)

         cgcTTTACTACCTGCATATTCTAAGTGAATACCGTCATATGCAAAGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTAcc                                                                                                                            >  2:14007/1‑141 (MQ=255)

          gcTTTACTACCTGCATATTCTAAGTGAATACCGTCATATGCAAAGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTTATTTATTCTACCt                                                                                                                           <  2:645017/141‑1 (MQ=255)

          gcTTTACTACCTGCATATTCTAAGTGAATACCGTCATATGCAAAGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCt                                                                                                                           <  4:134715/141‑1 (MQ=255)

            tttACTACCTGCATATTCTAAGTGAATACCGTCATATGCAAAGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTc                                                                                                                         <  1:211945/141‑1 (MQ=255)

             ttACTACCTGCATATTCTAAGTGAATACCGTCATATGCAAAGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCa                                                                                                                        <  4:189170/141‑1 (MQ=255)

              tactacCTGCATATTCTAAGTGAATACCGTCATATGCAAAGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCAt                                                                                                                       <  1:763563/141‑1 (MQ=255)

              tactacCTGCATATTCTAAGTGAATACCGTCATATGCAAAGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCAt                                                                                                                       <  1:309581/141‑1 (MQ=255)

                ctacCTGCATATTCTAAGTGAATACCGTCATATGCAAAGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATaa                                                                                                                     >  2:396403/1‑141 (MQ=255)

                ctacCTGCATATTCTAAGTGAATACCGTCATATGCAAAGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATaa                                                                                                                     >  3:191899/1‑141 (MQ=255)

                    cTGCATATTCTAAGTGAATACCGTCATATGCAAAGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAAtctc                                                                                                                 <  1:634908/141‑1 (MQ=255)

                       cATATTCTAAGTGAATACCGTCATATGCAAAGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAg                                                                                                              <  1:385477/141‑1 (MQ=255)

                        atatTCTAAGTGAATACCGTCATATGCAAAGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAAGTAATTTATTCTACCTTCATAATCTCTAgg                                                                                                             <  2:858609/141‑1 (MQ=255)

                         tatTCTAAGTGAATACCGTCATATGCAAAGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTTATTTATTCTACCTTCATAATCTCTAGGt                                                                                                            <  2:586342/141‑1 (MQ=255)

                         tatTCTAAGTGAATACCGTCATATGCAAAGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGt                                                                                                            >  1:17169/1‑141 (MQ=255)

                         tatTCTAAGTGAATACCGTCATATGCAAAGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGt                                                                                                            <  1:549304/141‑1 (MQ=255)

                            tCTAAGTGAATACCGTCATATGCAAAGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTAct                                                                                                         <  2:492786/141‑1 (MQ=255)

                             cTAAGTGAATACCGTCATATGCAAAGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTActc                                                                                                        >  1:101919/1‑141 (MQ=255)

                             cTAAGTGAATACCGTCATATGCAAAGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTActc                                                                                                        <  2:318695/141‑1 (MQ=255)

                               aaGTGAATACCGTCATATGCAAAGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTa                                                                                                      >  1:203172/1‑141 (MQ=255)

                                  tGAATACCGTCATATGCAAAGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATa                                                                                                   <  2:488231/141‑1 (MQ=255)

                                   gAATACCGTCATATGCAAAGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAg                                                                                                  >  4:252920/1‑141 (MQ=255)

                                    aaTACCGTCATATGCAAAGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGa                                                                                                 >  2:366257/1‑141 (MQ=255)

                                     aTACCGTCATATGCAAAGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGaa                                                                                                <  2:72835/141‑1 (MQ=255)

                                       aCCGTCATATGCAAAGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAAc                                                                                              <  2:582129/141‑1 (MQ=255)

                                       aCCGTCATATGCAAAGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAAc                                                                                              >  2:825939/1‑141 (MQ=255)

                                       aCCGTCATATGCAAAGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAAc                                                                                              >  1:743847/1‑141 (MQ=255)

                                       aCCGTCATATGCAAAGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAAc                                                                                              <  2:609393/141‑1 (MQ=255)

                                         cctCATATGCAAAGTATTCCGGATGACCTGCAGCAGCTTTCTACCCATCGACTAGACGAAAATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTa                                                                                            <  1:436755/139‑1 (MQ=255)

                                         cGTCATATGCAAAGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTa                                                                                            >  2:522038/1‑141 (MQ=255)

                                          gTCATATGCAAAGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGC‑TTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTaaa                                                                                          <  2:819766/141‑1 (MQ=255)

                                           tCATATGCAAAGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTaaa                                                                                          <  4:298025/141‑1 (MQ=255)

                                             atatGCAAAGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAata                                                                                        <  2:46148/141‑1 (MQ=255)

                                                tGCAAAGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAatatat                                                                                     <  1:468207/141‑1 (MQ=255)

                                                    aaGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCt                                                                                 >  2:540293/1‑141 (MQ=255)

                                                     aGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTg                                                                                <  1:114113/141‑1 (MQ=255)

                                                       tATTCCGGATGACCTGCAGAAGCTTTATACCAGTCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCt                                                                              <  1:493762/141‑1 (MQ=255)

                                                        aTTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCtt                                                                             >  2:573705/1‑141 (MQ=255)

                                                        aTTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCtt                                                                             >  1:4487/1‑141 (MQ=255)

                                                          tCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCtttt                                                                           >  3:172456/1‑141 (MQ=255)

                                                           ccGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTTATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTc                                                                          >  2:149243/1‑141 (MQ=255)

                                                           ccGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTc                                                                          >  1:41191/1‑141 (MQ=255)

                                                            cGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATAAGTCTGCTTTTcc                                                                         >  1:311761/1‑141 (MQ=255)

                                                            cGGATGACCTGCAGAAGCTTTATACCAATCGAATAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTcc                                                                         <  4:59238/141‑1 (MQ=255)

                                                                   ccTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTa                                                                  >  3:69010/1‑141 (MQ=255)

                                                                     tGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATc                                                                >  2:150353/1‑141 (MQ=255)

                                                                      gCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATcc                                                               >  1:54226/1‑141 (MQ=255)

                                                                         gAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAAt                                                            <  2:133055/141‑1 (MQ=255)

                                                                          aaGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATa                                                           <  2:419815/141‑1 (MQ=255)

                                                                            gCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGt                                                         <  2:383149/141‑1 (MQ=255)

                                                                            gCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGt                                                         >  3:260879/1‑141 (MQ=255)

                                                                            gCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGt                                                         >  2:436250/1‑141 (MQ=255)

                                                                            gCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGt                                                         <  2:292617/141‑1 (MQ=255)

                                                                             cTTTATACCAATCGACTAGATGTACAATAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCGTAATCTCTAGGTACTCTAATAGATACTATACATATGTCTGCTTTTCCAAAACTATCCAATAGtt                                                        <  4:109317/141‑1 (MQ=255)

                                                                               ttATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTTATTTATTCTACCGTCGTAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCa                                                      <  1:824728/141‑1 (MQ=255)

                                                                                 ataCCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCAtt                                                    <  2:732141/141‑1 (MQ=255)

                                                                                 ataCCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCAtt                                                    >  2:657585/1‑141 (MQ=255)

                                                                                  taCCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCAttt                                                   >  4:102250/1‑141 (MQ=255)

                                                                                    ccAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTaa                                                 <  2:317046/141‑1 (MQ=255)

                                                                                     cAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAAt                                                <  4:156317/141‑1 (MQ=255)

                                                                                     cAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAAt                                                <  4:167927/141‑1 (MQ=255)

                                                                                      aaTCGACTATATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAAtt                                               <  3:74863/141‑1 (MQ=255)

                                                                                          gACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAATTGATc                                           >  1:148735/1‑141 (MQ=255)

                                                                                           aCTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAATTGATCt                                          >  1:294118/1‑141 (MQ=255)

                                                                                            cTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAATTGATCtt                                         >  2:260957/1‑141 (MQ=255)

                                                                                            cTAGATGTACATTAGAGCGCTTTTAAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGa                                                                                                 >  2:277263/1‑85 (MQ=255)

                                                                                            cTAGATGTACATTAGAGCGCTTTTAAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGa                                                                                                 <  1:277263/85‑1 (MQ=255)

                                                                                              aGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAATTGATCTTTc                                       >  2:399507/1‑141 (MQ=255)

                                                                                               gATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAATTGATCTTTCg                                      >  1:333063/1‑141 (MQ=255)

                                                                                                    aCATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAAATAACGATAGGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAATTGATCTTTCGTAAAt                                 <  1:16135/141‑1 (MQ=255)

                                                                                                     cATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAATTGATCTTTCGTAAATg                                >  1:839620/1‑141 (MQ=255)

                                                                                                      aTTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAATTGATCTTTCGTAAATGc                               >  3:172658/1‑141 (MQ=255)

                                                                                                       ttAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAATTGATCTTTCGTAAATGcc                              <  2:122102/141‑1 (MQ=255)

                                                                                                        tAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAATTGATCTTTCGTAAATGccc                             >  4:346648/1‑141 (MQ=255)

                                                                                                         agagCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAATTGATCTTTCGTAAATGcccc                            <  2:367427/141‑1 (MQ=255)

                                                                                                         agagCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAATTGATCTTTCGTAAATGcccc                            >  3:310869/1‑141 (MQ=255)

                                                                                                           agCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAATTGATCTTTCGTAAATGCCCCAt                          >  1:239292/1‑141 (MQ=255)

                                                                                                            gcgcTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAATTGATCTTTCGTAAATGCCCCAtt                         <  4:286239/141‑1 (MQ=255)

                                                                                                             cgcTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAATTGATCTTTCGTAAATGCCCCAttt                        <  2:325608/141‑1 (MQ=255)

                                                                                                              gcTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAATTGATCTTTCGTAAATGCCCCATTTg                       >  2:385321/1‑141 (MQ=255)

                                                                                                                 tttCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAATTGATCTTTCGTAAATGCCCCATTTGTAc                    <  3:98475/141‑1 (MQ=255)

                                                                                                                 tttCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAATTGATCTTTCGTAAATGCCCCATTTGTAc                    <  3:19346/141‑1 (MQ=255)

                                                                                                                  ttCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAATTGATCTTTCGTAAATGCCCCATTTGTAcc                   <  1:291163/141‑1 (MQ=255)

                                                                                                                  ttCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAATTGATCTTTCGTAAATGCCCCATTTGTAcc                   >  2:631890/1‑141 (MQ=255)

                                                                                                                      gCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAATTGATCTTTCGTAAATGCCCCATTTGTACCAAGc               >  2:131543/1‑141 (MQ=255)

                                                                                                                       cTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAATTGATCTTTCGTAAATGCCCCATTTGTACCAAGct              <  1:647630/141‑1 (MQ=255)

                                                                                                                        tGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAATTGATCTTTCGTAAATGCCCCATTTGTACCAAGctc             >  2:665905/1‑141 (MQ=255)

                                                                                                                           cTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAATTGATCTTTCGTAAATGCCCCATTTGTACCAAGCTctac          >  1:330502/1‑141 (MQ=255)

                                                                                                                             cATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAATTGATCTTTCGTAAATGCCCCATTTGTACCAAGCTctacta        <  1:256390/141‑1 (MQ=255)

                                                                                                                              aTAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAATTGATCTTTCGTAAATGCCCCATTTGTACCAAGCTCtactac       <  2:744377/141‑1 (MQ=255)

                                                                                                                               tAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAATTGATCTTTCGTAAATGCCCCATTTGTACCAAGCTCTACTACa      <  2:525424/141‑1 (MQ=255)

                                                                                                                               tAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAATTGATCTTTCGTAAATGCCCCATTTGTACCAAGCTCTACTACa      <  3:97764/141‑1 (MQ=255)

                                                                                                                                  attaattaATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAATTGATCTTTCGTAAATGCCCCATTTGTACCAAGCTCTACTACAACt   >  1:486664/1‑141 (MQ=255)

                                                                                                                                   ttaattaaTTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAATTGATCTTTCGTAAATGCCCCATTTGTACCAAGCTCTACTACAACtt  <  1:553392/141‑1 (MQ=255)

ATCAGTCAGCGCTTTACTACCTGCATATTCTAAGTGAATACCGTCATATGCAAAGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTTATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAATTGATCTTTCGTAAATGCCCCATTTGTACCAAGCTCTACTACAACTT  >  USA300TCH1516_ALE/2709160‑2709431

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`