BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	469,786	A→G	G118G (GGA→GGG)	lpl2_6 →	putative lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	469,786	0	A	G	94.5%	297.3 / 3.2	91	G118G (GGA→GGG)	lpl2_6	putative lipoprotein
Reads supporting (aligned to +/- strand): ref base A (1/4); new base G (60/26); total (61/30)
Fisher's exact test for biased strand distribution p-value = 3.90e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01

CGAGATGGCGAATTTAAAAAGGGTGATAAAGGGATGTGGACGATATATACAGATTTCGCCAAAAGTAATAAACAAGGTGGATTGAGTAATGAAGGTATGGTCTTATACTTAGATAGAAATACACGGACTGCAAAGGGACATTATTTTGTTAAGACATTCTATAATAAGGGCAAATTCCCAGATAGAAAAAATTATAAAGTTGAAATGAAAAATAATAAAATTATCTTATTAGATAAAGTAGAAGATACAAATCTAAAAAAGAGAATAGAAAACTT  >  USA300TCH1516_ALE/469649‑469923

cGAGATGGCGAATTTAAAAAGGGTGATAAAGGGATGTGGACGATATATACAGATTTCGCCAAAAGTAATAAACAAGGTGGATTGAGTAATGAAGGTATGGTCTTATACTTAGATAGAAATACACGGACTGCAAAGGGGCat                                                                                                                                        >  2:275922/1‑141 (MQ=255)

cGAGATGGCGAATTTAAAAAGGGTGATAAAGGGATGTGGACGATATATACAGATTTCGCCAAAAGTAATAAACAAGGTGGATTGAGTAATGAAGGTATGGTCTTATACTTAGATAGAAATACACGGACTGCAAAGGGGCat                                                                                                                                        >  4:128172/1‑141 (MQ=255)

  agaTGGCGAATTTAAAAAGGGTGATAAAGGGATGTGGACGATATATACAGATTTCGCCAAAAGTAATAAACAAGGTGGATTGAGTAATGAAGGTATGGTCTTATACTTAGATAGAAATACACGGACTGCAACGGGGCatta                                                                                                                                      <  1:766421/141‑1 (MQ=255)

  agaTGGCGAATTTAAAAAGGGTGATAAAGGGATGTGGACGATATATACAGATTTCGCCAAAAGTAATAAACAAGGTGGATTGAGTAATGAAGGTATGGTCTTATACTTAGATAGAAATACACGGACTGCAAAGGGACatta                                                                                                                                      <  4:332025/141‑1 (MQ=255)

       gCGAATTTAAAAAGGGTGATAAAGGGATGTGGACGATATATACAGATTTCGCCAAAAGTAATAAACAAGGTGGATTGAGTAATGAAGGTATGGTCTTATACTTAGATAGAAATACACGGACTGCAAAGGGGCATTATTTTg                                                                                                                                 >  3:282799/1‑141 (MQ=255)

         gAATTTAAAAAGGGTGATAAAGGGATGTGGACGATATATACAGATTTCGCCAAAAGTAATAAACAAGGTGGATTGAGTAATGAAGGTATGGTCTTATACTTAGATAGAAATACACGGACTGCAAAGGGACATTATTTTGtt                                                                                                                               <  2:270000/141‑1 (MQ=255)

           aTTTAAAAAGGGTGATAAAGGGATGTGGACGATATATACAGATTTCGCCAAAAGTAATAAACAAGGTGGATTGAGTAATGAAGGTATGGTCTTATACTTAGATAGAAATACACGGACTGCAAAGGGGCATTATTTTGTTaa                                                                                                                             >  2:109007/1‑141 (MQ=255)

           aTTTAAAAAGGGTGATAAAGGGATGTGGACGATATATACAGATTTCGCCAAAAGTAATAAACAAGGTGGATTGAGTAATGAAGGTATGGTCTTATACTTAGATAGAAATACACGGACTGCAAAGGGGCATTATTTTGTTaa                                                                                                                             >  2:640256/1‑141 (MQ=255)

            tttAAAAAGGGTGATAAAGGGATGTGGACGATATATACAGATTTCGCCAAAAGTAATAAACAAGGTGGATTGAGTAATGAAGGTATGGTCTTATACTTAGATAGAAATACACGGACTGCAAAGGGGCATTATTTTGTTAAg                                                                                                                            <  3:79418/141‑1 (MQ=255)

             ttAAAAAGGGTGATAAAGGGATGTGGACGATATATACAGATTTCGCCAAAAGTAATAAACAAGGTGGATTGAGTAATGAAGGTATGGTCTTATACTTAGATAGAAATACACGGACTGCAAAGGGGCATTATTTTGTTAAGa                                                                                                                           <  4:44011/141‑1 (MQ=255)

                aaaaGGGTGATAAAGGGATGTGGACGATATATACAGATTTCGCCAAAAGTAATAAACAAGGTGGATTGAGTAATGAAGGTATGGTCTTATACTTAGATAGAAATACACGGACTGCAAAGGGGCATTATTTTGTTAAGACAt                                                                                                                        >  4:134887/1‑141 (MQ=255)

                  aaGGGTGATAAAGGGATGTGGACGATATATACAGATTTCGCCAAAAGTAATAAACAAGGTGGATTGAGTAATGAAGGTATGGTCTTATACTTAGATAGAAATACACGGACTGCAAAGGGGCATTATTTTGTTAAGACATTc                                                                                                                      <  1:833354/141‑1 (MQ=255)

                   aGGGTGATAAAGGGATGTGGACGATATATACAGATTTCGCCAAAAGTAATAAACAAGGTGGATTGAGTAATGAAGGTATGGTCTTATACTTAGATAGAAATACACGGACTGCAAAGGGGCATTATTTTGTTAAGACATTCt                                                                                                                     <  1:85279/141‑1 (MQ=255)

                        gATAAAGGGATGTGGACGATATATACAGATTTCGCCAAAAGTAATAAACAAGGTGGATTGAGTAATGAAGGTATGGTCTTATACTTAGATAGAAATACACGGACTGCAAAGGGGCATTATTTTGTTAAGACATTCTataat                                                                                                                >  1:574361/1‑141 (MQ=255)

                          tAAAGGGATGTGGACGATATATACAGATTTCGCCAAAAGTAATAAACAAGGTGGATTGAGTAATGAAGGTATGGTCTTATACTTAGATAGAAATACACGGACTGCAAAGGGGCATTATTTTGTTAAGACATTCTAtaataa                                                                                                              >  1:259444/1‑141 (MQ=255)

                             aGGGATGTGGACGATATATACAGATTTCGCCAAAAGTAATAAACAAGGTGGATTGAGTAATGAAGGTATGGTCTTATACTTAGATAGAAATACACGGACTGCAAAGGGGCATTATTTTGTTAAGACATTCTATAATAAggg                                                                                                           <  2:701121/141‑1 (MQ=255)

                              gggATGTGGACGATATATACAGATTTCGCCAAAAGTAATAAACAAGGTGGATTGAGTAATGAAGGTATGGTCTTATACTTAGATAGAAATACACGGACTGCAAAGGGGCATTATTTTGTTAAGACATTCTATAATAAGGGc                                                                                                          <  2:4493/141‑1 (MQ=255)

                              gggATGTGGACGATATATACAGATTTCGCCAAAAGTAATAAACAAGGTGGATTGAGTAATGAAGGTATGGTCTTATACTTAGATAGAAATACACGGACTGCAAAGGGGCATTATTTTGTTAAGACATTCTATAATAAGGGc                                                                                                          >  4:273482/1‑141 (MQ=255)

                                gATGTGGACGATATATACAGATTTCGCCAAAAGTAATAAACAAGGTGGATTGAGTAATGAAGGTATGGTCTTATACTTAGATAGAAATACACGGACTGCAAAGGGGCATTATTTTGTTAAGACATTCTATAATAAGGGCaa                                                                                                        <  2:398940/141‑1 (MQ=255)

                                    tgGACGATATATACAGATTTCGCCAAAAGTAATAAACAAGGTGGATTGAGTAATGAAGGTATGGTCTTATACTTAGATAGAAATACACGGACTGCAAAGGGGCATTATTTTGTTAAGACATTCTATAATAAGGGCAAATTc                                                                                                    >  3:181312/1‑141 (MQ=255)

                                    tgGACGATATATACAGATTTCGCCAAAAGTAATAAACAAGGTGGATTGAGTAATGAAGGTATGGTCTTATACTTAGATAGAAATACACGGACTGCAAAGGGGCATTATTTTGTTAAGACATTCTATAATAAGGGCAAATTc                                                                                                    >  1:3279/1‑141 (MQ=255)

                                        cGATATATACAGATTTCGCCAAAAGTAATAAACAAGGTGGATTGAGTAATGAAGGTATGGTCTTATACTTAGATAGAAATACACGGACTGCAAAGGGGCATTATTTTGTTAAGACATTCTATAATAAGGGCAAATTCCCAg                                                                                                >  1:330882/1‑141 (MQ=255)

                                         gATATATACAGATTTCGCCAAAAGTAATAAACAAGGTGGATTGAGTAATGAAGGTATGGTCTTATACTTAGATAGAAATACACGGACTGCAAAGGGGCATTATTTTGTTAAGACATTCTATAATAAGGGCAAATTCCCAGa                                                                                               <  4:323103/141‑1 (MQ=255)

                                         gATATATACAGATTTCGCCAAAAGTAATAAACAAGGTGGATTGAGTAATGAAGGTATGGTCTTATACTTAGATAGAAATACACGGACTGCAAAGGGGCATTATTTTGTTAAGACATTCTATAATAAGGGCAAATACCCAGa                                                                                               <  4:115855/141‑1 (MQ=255)

                                           tatataCAGATTTCGCCAAAAGTAATAAACAAGGTGGATTGAGTAATGCAGGTATGGACTTATACATAGATAGAAATACACGGACTGCAAAGGGGCATTATTTTGTTAAGACATTCTATAATAAGGGCAAATTCCCAGATa                                                                                             <  1:171661/141‑1 (MQ=255)

                                             tataCAGATTTCGCCAAAAGTAATAAACAAGGTGGATTGAGTAATGAAGGTATGGTCTTATACTTAGATAGAAATACACGGACTGCAAAGGGGCATTATTTTGTTAAGACATTCTATAATAAGGGCAAATTCCCAGATAGa                                                                                           >  2:305901/1‑141 (MQ=255)

                                                 cAGATTTCGCCAAAAGTAATAAACAAGGTGGATTGAGTAATGAAGGTATGGTCTTATACTTAGATAGAAATACACGGACTGCAAAGGGGCATTATTTTGTTAAGACATTCTATAATAAGGGCAAATTCCCAGATAGaaaaa                                                                                       <  2:421144/141‑1 (MQ=255)

                                                     tttCGCCAAAAGTAATAAACAAGGTGGATTGAGTAATGAAGGTATGGTCTTATACTTAGATAGAAATACACGGACTGCAAAGGGGCATTATTTTGTTAAGACATTCTATAATAAGGGCAAATTCCCAGATAGAAAAAATta                                                                                   >  4:271138/1‑141 (MQ=255)

                                                      ttCGCCAAAAGTAATAAACAAGGTGGATTGAGTAATGAAGGTATGGTCTTATACTTAGATAGAAATACACGGACTGCAAAGGGGCATTATTTTGTTAAGACATTCTATAATAAGGGCAAATTCCCAGATAGAAAAAATtat                                                                                  <  4:169660/141‑1 (MQ=255)

                                                        cGCCAAAAGTAATAAACAAGGTGGATTGAGTAATGAAGGTATGGTCTTATACTTAGATAGAAATACACGGACTGCAAAGGGGCATTATTTTGTTAAGACATTCTATAATAAGGGCAAATTCCCAGATAGAAAAAATTATaa                                                                                <  3:147475/141‑1 (MQ=255)

                                                        cGCCAAAAGTAATAAACAAGGTGGATTGAGTAATGAAGGTATGGTCTTATACTTAGATAGAAATACACGGACTGCAAAGGGGCATTATTTTGTTAAGACATTCTATAATAAGGGCAAATTCCCAGATAGAAAAAATTATaa                                                                                >  2:566745/1‑141 (MQ=255)

                                                         gCCAAAAGTAATAAACAAGGTGGATTGAGTAATGAAGGTATGGTCTTATACTTAGATAGAAATACACGGACTGCAAAGGGGCATTATTTTGTTAAGACATTCTATAATAAGGGCAAATTCCCAGATAGAAAAAATTATaaa                                                                               >  1:381004/1‑141 (MQ=255)

                                                         gCCAAAAGTAATAAACAAGGTGGATTGAGTAATGAAGGTATGGTCTTATACTTAGATAGAAATACACGGACTGCAAAGGGGCATTATTTTGTTAAGACATTCTATAATAAGGGCAAATTCCCAGATAGAAAAAATTATaaa                                                                               >  2:720219/1‑141 (MQ=255)

                                                           cAAAAGTAATAAACAAGGTGGATTGAGTAATGAAGGTATGGTCTTATACTTAGATAGAAATACACGGACTGCAAAGGGGCATTATTTTGTTAAGACATTCTATAATAAGGGCAAATTCCCAGATAGAAAAAATTATAAAGt                                                                             <  3:321355/141‑1 (MQ=255)

                                                              aaGTAATAAACAAGGTGGATTGAGTAATGAAGGTATGGTCTTATACTTAGATAGAAATACACGGACTGCAAAGGGGCATTATTTTGTTAAGACATTCTATAATAAGGGCAAATTCCCAGATAGAAAAAATTATAAAGTtga                                                                          >  1:371465/1‑141 (MQ=255)

                                                                gTAATAAACAAGGTGGATTGAGTAATGAAGGTATGGTCTTATACTTAGATAGAAATACACGGACTGCAAAGGGGCATTATTTTGTTAAGACATTCTATAATAAGGGCAAATTCCCAGATAGAAAAAATTATAAAGTagaaa                                                                        >  2:640234/1‑141 (MQ=255)

                                                                  aatttaCAAGGTGGATTGAGTAATGAAGGTATGGTCTTATACTTAGATAGAAATACACGGACTGCAAAGGGGCATTATTTTGTTAAGACATTCTATAATAAGGGCAAATTCCCAGATAGAAAAAATTATAAAGTtgaaatg                                                                      >  2:530219/6‑141 (MQ=255)

                                                                  aataaACAAGGTGGATTGAGTAATGAAGGTATGGTCTTATACTTAGATAGAAATACACGGACTGCAAAGGGGCATTATTTTGTTAAGACATTCTATAATAAGGGCAAATTCCCAGATAGAAAAAATTATAAAGTtgaaatg                                                                      >  1:12673/1‑141 (MQ=255)

                                                                   ataaACAAGGTGGATTGAGTAATGAAGGTATGGTCTTATACTTAGATAGAAATACACGGACTGCAAAGGGGCATTATTTTGTTAAGACATTCTATAATAAGGGCAAATTCCCAGATAGAAAAAATTATAAAGTtgaaatga                                                                     >  2:381682/1‑141 (MQ=255)

                                                                   ataaACAAGGTGGATTGAGTAATGAAGGTATGGTCTTATACTTAGATAGAAATACACGGACTGCAAAGGGACATTATTTTGTTAAGACATTCTATAATAAGGGCAAATTCCCAGATAGAAAAAATTATAAAGTtgaaatga                                                                     >  1:489112/1‑141 (MQ=255)

                                                                    taaACAAGGTGGATTGAGTAATGAAGGTATGGTCTTATACTTAGATAGAAATACACTGACTGCAAAGGGGCATTATTTTGTTAAGACATTCTATAATAAGGGCAAATTCCCAGATAGAAAAAATTATAAAGTtgaaatgaa                                                                    >  4:127160/1‑141 (MQ=255)

                                                                    taaACAAGGTGGATTGAGTAATGAAGGTATGGTCTTATACTTAGATAGAAATACACGGACTGCAAAGGGGCATTATTTTGTTAAGACATTCTATAATAAGGGCAAATTCCCAGATAGAAAAAATTATAAAGTtgaaatgaa                                                                    >  1:794101/1‑141 (MQ=255)

                                                                    taaACAAGGTGGATTGAGTAATGAAGGTATGGTCTTATACTTAGATAGAAATACACGGACTGCAAAGGGGCATTATTTTGTTAAGACATTCTATAATAAGGGCAAATTCCCAGATAGAAAAAATTATAAAGTtgaaatgaa                                                                    >  1:675137/1‑141 (MQ=255)

                                                                    taaACAAGGTGGATTGAGTAATGAAGGTATGGTCTTATACTTAGATAGAAATACACGGACTGCAAAGGGGCATTATTTTGTTAAGACATTCTATAATAAGGGCAAATTCCCAGATAGAAAAAATTATAAAGTtgaaatgaa                                                                    >  1:415105/1‑141 (MQ=255)

                                                                     aaaCAAGGTGGATTGAGTAATGAAGGTATGGTCTTATACTTAGATAGAAATACGCGGACTGCAAAGGGGCATTATTTTGTTAAGACATTCTATAATAAGGGCAAATTCCCAGATAGAAAAAATTATAAAGTtgaaatgaaa                                                                   >  1:659672/1‑141 (MQ=255)

                                                                     aaaCAAGGTGGATTGAGTAATGAAGGTATGGTCTTATACTTAGATAGAAATACACGGACTGCAAAGGGGCATTATTTTGTTAAGACATTCTATAATAAGGGCAAATTCCCAGATAGAAAAAATTATAAAGTtgaaatgaaa                                                                   >  2:548355/1‑141 (MQ=255)

                                                                     aaaCAAGGTGGATTGAGTAATGAAGGTATGGTCTTATACTTAGATAGAAATACACGGACTGCAAAGGGGCATTATTTTGTTAAGACATTCTATAATAAGGGCAAATTCCCAGATAGAAAAAATTATAAAGTtgaaatgaaa                                                                   >  1:233414/1‑141 (MQ=255)

                                                                        cAAGGTGGATTGAGTAATGAAGGTATGGTCTTATACTTAGATAGAAATACACGGACTGCAAAGGGGCATTATTTTGTTAAGACATTCTATAGTAAGGGCAAATTCCCAGATAGAAAAAATTATAAAGTTGAAATGaaaaaa                                                                >  4:236488/1‑140 (MQ=255)

                                                                        cAAGGTGGATTGAGTAATGAAGGTATGGTCTTATACTTAGATAGAAATACACGGACTGCAAAGGGGCATTATTTTGTTAAGACATTCTATAATAAGGGCAAATTCCCAGATAGAAAAAATTATAAAGTTGAAATGAAAaat                                                                <  1:305157/141‑1 (MQ=255)

                                                                           cgtcgattgattAATGAAGGTATGGTCTTATACTTAGATAGAAATACACGGACTGCAAAGGGGCATTATTTTGTTAAGACATTCTATAATAAGGGCAAATTCCCAGATAGAAAAAATTATAAAGTTGAAATGAAAaataat                                                             <  4:292855/137‑1 (MQ=255)

                                                                              ggATTGAGTAATGAAGGTATGGTCTTATACTTAGATAGAAATACACGGACTGCAAAGGGGCATTATTTTGTTAAGACATTCTATAATAAGGGCAAATTCCCAGATAGAAAAAATTATAAAGTTGAAATGAAAAATAATaaa                                                          >  3:241370/1‑141 (MQ=255)

                                                                               gATTGAGTAATGAAGGTATGGTCTTATACTTAGATAGAAATACACGGACTGCAAAGGGACATTATTTTGTTAAGACATTCTATAATAAGGGCAAATTCCCAGATAGAAAAAATTATAAAGTTGAAATGAAAAATAATaaaa                                                         <  3:10208/141‑1 (MQ=255)

                                                                                     gTAATGAAGGTATGGTCTTATACTTAGATAGAAATACACGGACTGCAAAGGGGCATTATTTTGTTAAGACATTCTATAATAAGGGCAAATTCCCAGATAGAAAAAATTATAAAGTTGAAATGAAAAATAATAAAATTATCt                                                   >  3:13718/1‑141 (MQ=255)

                                                                                       aaTGAAGGTATGGGCTTATACTTAGATAGAAATACACGGACTGCAAAGGGGCATTATTTTGTTAAGACATTCTATAATAAGGGCAAATTCCCAGATAGAAAAAATTATAAAGTTGAAATGAAAAATAATAAAATTATCtta                                                 <  4:45402/141‑1 (MQ=255)

                                                                                        aTGAAGGTATGGTCTTATACTTAGATAGAAATACACGGACTGCAAAGGGGCATTATTTTGTTAAGACATTCTATAATAAGGGCAAATTCCCAGATAGAAAAAATTATAAAGTTGAAATGAAAAATAATAAAATTATCttat                                                >  3:357908/1‑141 (MQ=255)

                                                                                        aTGAAGGTATGGTCTTATACTTAGATAGAAATACACGGACTGCAAAGGGGCATTATTTTGTTAAGACATTCTATAATAAGGGCAAATAAACAGATAGAAAAAATTATAAAGTTGAAATGAAAAATAATAAAATTATCttat                                                <  2:584692/141‑1 (MQ=255)

                                                                                           aaGGTATGGTCTTATACTTAGATAGAAATACACGGACTGCAAAGGGGCATTATTTTGTTAAGACATTCTATAATAAGGGCAAATTCCCAGATAGAAAAAATTATAAAGTTGAAATGAAAAATAATAAAATTATCTTATTAg                                             >  1:666046/1‑141 (MQ=255)

                                                                                             ggTATGGTCTTATACTTAGATAGAAATACACGGACTGCAAAGGGGCATTATTTTGTTAAGACATTCTATAATAAGGGCAAATTCCCAGATAGAAAAAATTATAAAGTTGAAATGAAAAATAATAAAATTATCTTATTAGAt                                           >  2:699537/1‑141 (MQ=255)

                                                                                             ggTATGGTCTTATACTTAGATAGAAATACACGGACTGCAAAGGGGCATTATTTTGTTAAGACATTCTATAATAAGGGCAAATTCCCAGATAGAAAAAATTATAAAGTTGAAATGAAAAATAATAAAATTATCTTATTAGAt                                           >  2:609697/1‑141 (MQ=255)

                                                                                               tATGGTCTTATACTTAGATAGAAATACACGGACTGCAAAGGGGCATTATTTTGTTAAGACATTCTATAATAAGGGCAAATTCCCAGATAGAAAAAATTATAAAGTTGAAATGAAAAATAATAAAATTATCTTATTAGATaa                                         >  3:328892/1‑141 (MQ=255)

                                                                                                 tGGTCTTATACTTAGATAGAAATACACGGACTGCAAAGGGGCATTATTTTGTTAAGACATTCTATAATAAGGGCAAATTCCCAGATAGAAAAAATTATAAAGTTGAAATGAAAAATAATAAAATTATCTTATTAGATAAAg                                       >  4:307366/1‑141 (MQ=255)

                                                                                                 tGGTCTTATACTTAGATAGAAATACACGGACTGCAAAGGGGCATTATTTTGTTAAGACATTCTATAATAAGGGCAAATTCCCAGATAGAAAAAATTATAAAGTTGAAATGAAAAATAATAAAATTATCTTATTAGATAAAg                                       >  2:718043/1‑141 (MQ=255)

                                                                                                  ggTCTTATACTTAGATAGAAATACACGGACTGCAAAGGGGCATTATTTTGTTAAGACATTCTATAATAAGGGCAAATTCCCAGATAGAAAAAATTATAAAGTTGAAATGAAAAATAATAAAATTATCTTATTAGATAAAGt                                      <  2:369721/141‑1 (MQ=255)

                                                                                                   gTCTTATACTTAGATAGAAATACACGGACTGCAAAGGGGCATTATTTTGTTAAGACATTCTATAATAAGGGCAAATTCCCAGATAGAAAAAATTATAAAGTTGAAATGAAAAATAATAAAATTATCTTATTAGATAAAGTa                                     >  2:274374/1‑141 (MQ=255)

                                                                                                     cTTATACTTAGATAGAAATACACGGACTGCAAAGGGGCATTATTTTGTTAAGACATTCTATAATAAGGGCAAATTCCCAGATAGAAAAAATTATAAAGTTGAAATGAAAAATAATAAAATTATCTTATTAGATAAAGTaga                                   >  2:392199/1‑141 (MQ=255)

                                                                                                     cTTATACTTAGATAGAAATACACGGACTGCAAAGGGGCATTATTTTGTTAAGACATTCTATAATAAGGGCAAATTCCCAGATAGAAAAAATTATAAAGTTGAAATGAAAAATAATAAAAAAATCTTATTAGATAAAGTaga                                   >  2:392257/1‑141 (MQ=255)

                                                                                                         taCTTAGATAGAAATACACGGACTGCAAAGGGGCATTATTTTGTTAAGACATTCTATAATAAGGGCAAATTCCCAGATAGAAAAAATTATAAAGTTGAAATGAAAAATAATAAAATGATCTTATTAGATAAAGTAGAAGAt                               >  1:406926/1‑141 (MQ=255)

                                                                                                            ttAGATAGAAATACACGGACTGCAAAGGGGCATTATTTTGTTAAGACATTCTATAATAAGGGCAAATTCCCAGATAGAAAAAATTATAAAGTTGAAATGAAAAATAATAAAATTATCTTATTAGATAAAGTAGAAGATACa                            >  1:725618/1‑141 (MQ=255)

                                                                                                            ttAGATAGAAATACACGGACTGCAAAGGGGCATTATTTTGTTAAGACATTCTATAATAAGGGCAAATT‑CCAGATAGAAAAAATTATAAAGTTGAAATGAAAAATAATAAAATTATCTTATTAGATAAAGTAGAAGATACaa                           >  1:805396/1‑141 (MQ=255)

                                                                                                            ttAGATAGAAATACACGGACTGCAAAGGGGCATTATTTTGTTAAGACATTCTATAATAAGGGCAAATT‑CCAGATAGAAAAAATTATAAAGTTGAAATGAAAAATAATAAAATTATCTTATTAGATAAAGTAGAAGATACaa                           >  1:831274/1‑141 (MQ=255)

                                                                                                             tagatagaAATACACGGACTGCAAAGGGGCATTATTTTGTTAAGACATTCTATAATAAGGGCAAATTCCCAGATAGAAAAAATTATAAAGTTGAAATGAAAAATAATAAAATTATCTTATTAGATAAAGTAGAAGATACaa                           >  1:510800/1‑141 (MQ=255)

                                                                                                              agatagaAATACACGGACTGCAAAGGGGCATTATTTTGTTAAGACATTCTATAATAAGGGCAAATTCCCAGATAGAAAAAATTATAAAGTTGAAATGAAAAATAATAAAATTATCTTATTAGATAAAGTAGAAGATACaaa                          <  2:553742/141‑1 (MQ=255)

                                                                                                               gatagaAATACACGGACTGCAAAGGGGCATTATTTTGTTAAGACATTCTATAATAAGGGCAAATTCCCAGATAGAAAAAATTATAAAGTTGAAATGAAAAATAATAAAATTATCTTATTAGATAAAGTAGAAGATACAAAt                         >  1:203321/1‑141 (MQ=255)

                                                                                                                atagaAATACACGGACTGCAAAGGGGCATTATTTTGTTAAGACATTCTATAATAAGGGCAAATTCCCAGATAGAAAAAATTATAAAGTTGAAATGAAAAATAATAAAATTATCTTATTAGATAAAGTAGAAGATACAAATc                        <  3:322272/141‑1 (MQ=255)

                                                                                                                atagaAAAACACGAAATGAAAAGAGGAATTAAATTGTTAAAACAATAAATAAAAAAGACAAATACCAAGATAGAAAAAATTATAAAATAAAAATAAAAAATAATAAAATTATCTTATTAGATAAAGTAGAAGAAACaaaac                        <  2:487934/141‑3 (MQ=12)

                                                                                                                 tagaAATACACGGACTGCAAAGGGGCATTATTTTGTTAAGACATTCTATAATAAGGGCAAATTCCCAGATAGAAAAAATTATAAAGTTGAAATGAAAAATAATAAAATTATCTTATTAGATAAAGTAGAAGATACAAATCt                       >  2:446341/1‑141 (MQ=255)

                                                                                                                    aaaTACACGGACTGCAAAGGGGCATTATTTTGTTAAGACATTCTATAATAAGGGCAAATTCCCAGATAGAAAAAATTATAAAGTTGAAATGAAAAATAATAAAATTATCTTATTAGATAAAGTAGAAGATACAAATCTaaa                    >  2:601087/1‑141 (MQ=255)

                                                                                                                     aaTACACGGACTGCAAAGGGGCATTATTTTGTTAAGACATTCTATAATAAGGGCAAATTCCCAGATAGAAAAAATTATAAAGTTGAAATGAAAAATAATAAAATTATCTTATTAGATAAAGTAGAAGATACAAATCTaaaa                   >  2:825679/1‑141 (MQ=255)

                                                                                                                          acGGACTGCAAAGGGGCATTATTTTGTTAAGACATTCTATAATAAGGGCAAATTCCCAGATAGAAAAAATTATAAAGTTGAAATGAAAAATAATAAAATTATCTTATTAGATAAAGTAGAAGATACAAATCTAAAAAagag              <  1:766365/141‑1 (MQ=255)

                                                                                                                          acGGACTGCAAAGGGGCATTATTTTGTTAAGACATTCTATAATAAGGGCAAATTCCCAGATAGAAAAAATTATAAAGTTGAAATGAAAAATAATAAAATTATCTTATTAGATAAAGTAGAAGATACAAATCTAAAAAagag              >  1:743908/1‑141 (MQ=255)

                                                                                                                           cGGACTGCAAAGGGGCATTATTTTGTTAAGACATTCTATAATAAGGGCAAATTCCCAGATAGAAAAAATTATAAAGTTGAAATGAAAAATAATAAAATTATCTTATTAGATAAAGTAGAAGATACAAATCTAAAAAAgaga             >  1:857858/1‑141 (MQ=255)

                                                                                                                           cGGACTGCAAAGGGGCATTATTTTGTTAAGACATTCTATAATAAGGGCAAATTCCCAGATAGAAAAAATTATAAAGTTGAAATGAAAAATAATAAAATTATCTTATTAGATAAAGTAGAAGATACAAATCTAAAAAAgaga             >  3:114477/1‑141 (MQ=255)

                                                                                                                             gACTGCAAAGGGGCATTATTTTGTTAAGACATTCTATAATAAGGGCAAATTCCCAGATAGAAAAAATTATAAAGTTGAAATGAAAAATAATAAAATTATCTTATTAGATAAAGTAGAAGATACAAATCTAAAAAAGAGAAt           >  2:840212/1‑141 (MQ=255)

                                                                                                                              aCTGCAAAGGGGCATTATTTTGTTAAGACATTCTATAATAAGGGCAAATTCCCAGATAGAAAAAATTATAAAGTTGAAATGAAAAATAATAAAATTATCTTATTAGATAAAGTAGAAGATACAAATCTAAAAAAGAGAATa          >  2:149482/1‑141 (MQ=255)

                                                                                                                               cTGCAAAGGGGCATTATTTTGTTAAGACATTCTATAATAAGGGCAAATTCCCAGATAGAAAAAATTATAAAGTTGAAATGAAAAATAATAAAATTATCTTATTAGATAAAGTAGAAGATACAAATCTAAAAAAGAGAATAg         >  3:19055/1‑141 (MQ=255)

                                                                                                                               cTGCAAAGGGGCATTATTTTGTTAAGACATTCTATAATAAGGGCAAATTCCCAGATAGAAAAAATTATAAAGTTGAAATGAAAAATAATAAAATTATCTTATTAGATAAAGTAGAAGATACAAATCTAAAAAAGAGAATAg         <  1:110824/141‑1 (MQ=255)

                                                                                                                                   aaaGGGGCATTATTTTGTTAAGACATTCTATAATAAGGGCAAATTCCCAGATAGAAAAAATTATAAAGTTGAAATGAAAAATAATAAAATTATCTTATTAGATAAAGTAGAAGATACAAATCTAAAAAAGAGAATAGaaaa     >  2:613861/1‑141 (MQ=255)

                                                                                                                                    aaGGGGCATTATTTTGTTAAGACATTCTATAATAAGGGCAAATTCCCAGATAGAAAAAATTATAAAGTTGAAATGAAAAATAATAAAATTATCTTATTAGATAAAGTAGAAGATACAAATCTAAAAAAGAGAATAGAAAAc    >  4:261907/1‑141 (MQ=255)

                                                                                                                                     aGGGGCATTATTTTGTTAAGACATTCTATAATAAGGGCAAATTCCCAGATAGAAAAAATTATAAAGTTGAAATGAAAAATAATAAAATTATCTTATTAGATAAAGTAGAAGATACAAATCTAAAAAAGAGAATAGAAAACt   >  4:173257/1‑141 (MQ=255)

                                                                                                                                     aGGGGCATTATTTTGTTAAGACATTCTATAATAAGGGCAAATTCCCAGATAGAAAAAATTATAAAGTTAAAATGAAAAATAATAAAATTATCTTATTAGATAAAGTAGAAGATACAAATCTAAAAAAGAGAATAGAAAACt   <  2:605868/141‑1 (MQ=255)

                                                                                                                                      ggggCATTATTTTGTTAAGACATTCTATAATAAGGGCAAATTCCCAGATAGAAAAAATTATAAAGTTGAAATGAAAAATAATAAAATTATCTTATTAGATAAAGTAGAAGATACAAATCTAAAAAAGAGAATAGAAAACtt  <  1:612814/141‑1 (MQ=255)

                                                                                                                                      gggACATTCTTTTGTTAAGACATTATATAAAAAATGAAAAATACAAGATAAAAAAAATTATAAAATTAAAATAAAAAATAATAAAATTATCTTATTAGATAAAGTAGAAGATACAAATCTAAAAAAGAGAATAGAAAACtt  <  2:735821/141‑1 (MQ=14)

CGAGATGGCGAATTTAAAAAGGGTGATAAAGGGATGTGGACGATATATACAGATTTCGCCAAAAGTAATAAACAAGGTGGATTGAGTAATGAAGGTATGGTCTTATACTTAGATAGAAATACACGGACTGCAAAGGGACATTATTTTGTTAAGACATTCTATAATAAGGGCAAATTCCCAGATAGAAAAAATTATAAAGTTGAAATGAAAAATAATAAAATTATCTTATTAGATAAAGTAGAAGATACAAATCTAAAAAAGAGAATAGAAAACTT  >  USA300TCH1516_ALE/469649‑469923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`