breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
read file | reads | bases | passed filters | average | longest | mapped | |
---|---|---|---|---|---|---|---|
errors | SNFR_17_172_1_S191_S1999_L006_R1_001.good.fq | 872,157 | 121,401,233 | 100.0% | 139.2 bases | 141 bases | 96.6% |
errors | SNFR_17_172_1_S191_S1999_L006_R2_001.good.fq | 872,157 | 121,401,233 | 100.0% | 139.2 bases | 141 bases | 95.2% |
errors | SNFR_17_172_1_S191_S1999_L008_R1_001.good.fq | 372,957 | 51,842,638 | 100.0% | 139.0 bases | 141 bases | 96.6% |
errors | SNFR_17_172_1_S191_S1999_L008_R2_001.good.fq | 372,957 | 51,842,638 | 100.0% | 139.0 bases | 141 bases | 93.5% |
total | 2,490,228 | 346,487,742 | 100.0% | 139.1 bases | 141 bases | 95.6% |
seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
---|---|---|---|---|---|---|---|
coverage | distribution | USA300TCH1516_ALE | 2,872,915 | 114.8 | 1.6 | 99.9% | Staphylococcus species strain strain. |
coverage | distribution | CP000731 | 27,041 | 3.5 | 1.0 | 0.0% | Staphylococcus aureus subsp. aureus USA300_TCH1516 plasmid |
coverage | distribution | NC_012417 | 3,125 | 82.4 | 1.1 | 0.1% | Staphylococcus aureus subsp. aureus USA300_TCH1516 plasmid |
total | 2,903,081 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
option | limit | actual |
---|---|---|
Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 52087 |
Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 3 |
Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 352 |
Total length of all junction candidates (factor times the reference genome length) | ≤ 0.1 | 0.035 |
reference sequence | pr(no read start) |
---|---|
USA300TCH1516_ALE | 0.70031 |
CP000731 | 0.98768 |
NC_012417 | 0.78133 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
option | value |
---|---|
Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 3 |
Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 1 |
option | value |
---|---|
Mode | Consensus/Mixed Base |
Ploidy | 1 (haploid) |
Consensus mutation E-value cutoff | 10 |
Consensus frequency cutoff | 0.75 |
Consensus minimum variant coverage each strand | OFF |
Consensus minimum total coverage each strand | OFF |
Consensus minimum variant coverage | OFF |
Consensus minimum total coverage | OFF |
Polymorphism E-value cutoff | 10 |
Polymorphism frequency cutoff | 0.2 |
Polymorphism minimum variant coverage each strand | OFF |
Polymorphism minimum total coverage each strand | OFF |
Polymorphism minimum variant coverage | OFF |
Polymorphism minimum total coverage | OFF |
Polymorphism bias cutoff | OFF |
Predict indel polymorphisms | YES |
Skip indel polymorphisms in homopolymers runs of | OFF |
Skip base substitutions when they create a homopolymer flanked on each side by | OFF |
program | version |
---|---|
bowtie2 | 2.3.4.1 |
R | 3.4.4 |
step | start | end | elapsed |
---|---|---|---|
Read and reference sequence file input | 21:34:16 25 May 2019 | 21:34:51 25 May 2019 | 35 seconds |
Read alignment to reference genome | 21:34:51 25 May 2019 | 21:40:30 25 May 2019 | 5 minutes 39 seconds |
Preprocessing alignments for candidate junction identification | 21:40:30 25 May 2019 | 21:41:04 25 May 2019 | 34 seconds |
Preliminary analysis of coverage distribution | 21:41:04 25 May 2019 | 21:42:16 25 May 2019 | 1 minute 12 seconds |
Identifying junction candidates | 21:42:16 25 May 2019 | 21:42:45 25 May 2019 | 29 seconds |
Re-alignment to junction candidates | 21:42:45 25 May 2019 | 21:44:15 25 May 2019 | 1 minute 30 seconds |
Resolving best read alignments | 21:44:15 25 May 2019 | 21:45:12 25 May 2019 | 57 seconds |
Creating BAM files | 21:45:12 25 May 2019 | 21:46:15 25 May 2019 | 1 minute 3 seconds |
Tabulating error counts | 21:46:15 25 May 2019 | 21:46:41 25 May 2019 | 26 seconds |
Re-calibrating base error rates | 21:46:41 25 May 2019 | 21:46:43 25 May 2019 | 2 seconds |
Examining read alignment evidence | 21:46:43 25 May 2019 | 21:51:47 25 May 2019 | 5 minutes 4 seconds |
Polymorphism statistics | 21:51:47 25 May 2019 | 21:51:47 25 May 2019 | 0 seconds |
Output | 21:51:47 25 May 2019 | 21:52:02 25 May 2019 | 15 seconds |
Total | 17 minutes 46 seconds |