BRESEQ :: Evidence

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	USA300TCH1516_ALE	2085296 =	176 (1.040)	9 (0.060)	6/268	11.9	5.6%	coding (173/201 nt)	hlb_1	Phospholipase C
*	?	USA300TCH1516_ALE	= 2128343	144 (0.940)	9 (0.060)	6/268	11.9	5.6%	coding (1/825 nt)	hlb_2	Phospholipase C
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

Only 100 of 179 total aligned reads displayed.

TTCACTAAAAAAAGTTGCAACACTTGCATTAGCAAATTTATTATTAGTTGGTGCACTTACTGACAATAGTGCCAAAGCCGAATCTAAGAAAGATGATACTGATTTGAAGTTAGTTAGTCATAACGTTTATATGTTATCGACCGTTTTGTATCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCATTCAAAAAGTAAAACAGCATAATATCAAGGTTTATAACTTTATCATTATCAATAATACCTCATATAAAATAAAATTTTAGGGACTTTTTAGGGACTTTAAATTTAAAA  >  USA300TCH1516_ALE/2085150‑2085444

ttCACTAAAAAAAGTTGCAACACTTGCATTAGCAAATTTATTATTAGTTGGTGCACTTACTGACAATAGTGCCAAAGCCGAATCTAAGAAAGATGATACTGATTTGCAGTTAGTTAGTCATAACGTTTATATGTTATCGACCGTTTTGt                                                                                                                                                     <  1:245344/149‑1 (MQ=255)

ttCACTAAAAAAAGTTGCAACACTTGCATTAGCAAATTTATTATTAGTTGGTGCACTTACTGACAATAGTGCCAAAGCCGAATCTAAGAAAGATGATACTGATTTGAAGTTAGTTAGTCATAACGTTTATATGTTATCGACCGTTTTGt                                                                                                                                                     >  2:1082178/1‑149 (MQ=255)

  cACTAAAAAAAGTTGCAACACTTGCATTAGCAAGTTTATTATTAGTTGGTGCACTTACGGACAATAGTGCCAAAGCCGAATCTAAGAAAGATGATACTGATTTGAAGTTAGTTAGTCATAACGTTTATATGTTATCCACCGTTTTGTAt                                                                                                                                                   <  1:1171951/149‑1 (MQ=255)

  cACTAAAAAAAGTTGCAACACTTGCATTAGCAAATTTATTATTAGTTGGTGCACTTACTGACAATAGTGCCAAAGCCGAATCTAAGAAAGATGATACTGATTTGAAGTTAGTTAGTCATAACGTTTATATGTTATCGACCGTTTTGTAt                                                                                                                                                   <  1:522364/149‑1 (MQ=255)

   ccTAAAAAAAGTTGCAACACTTGCATTAGCAAATTTATTATTAGTTGGTGCACTTACTGACAATAGTGCCAAAGCCGAATCTAAGAAAGATGATACTGATTTGAAGTTAGTTAGTCATAACGTTTATATGTTATCGACCGTTTTGTATc                                                                                                                                                  <  1:725105/148‑1 (MQ=255)

   aCTAAAAAAAGTTGCAACACTTGCATTAGCAAATTTATTATTAGTTGGTGCACTTACTGACAATAGTGCCAAAGCCGAATCTAAGAAAGATGATACTGATTTGAAGTTAGTTAGTCATAACGTTTATATGTTATCGACCGTTTTGTATc                                                                                                                                                  <  1:1105075/149‑1 (MQ=255)

   aCTAAAAAAAGTTGCAACACTTGCATTAGCAAATTTATTATTAGTTGGTGCACTTACCGACAATAGTGCCAAAGCCGAATCTAAGAAAGATGATACTGATTTGAAGTTAGTTAGTCATAACGTTTATATGTTATCGACCGTTTTGTATc                                                                                                                                                  <  1:575341/149‑1 (MQ=255)

       aaaaaaGTTGCAACACTTGCATTAGCAAATTTATTATTAGTTGGTGCACTTACTGACAATAGTGCCAAAGCCGAATCTAAGAAAGATGATACTGATTTGAAGTTAGTTAGTCATAACGTTTATATGTTATCGACCGTTTTGTATCCaaa                                                                                                                                              <  2:36047/149‑1 (MQ=255)

       aaaaaaGTTGCAACACTTGCATTAGCAAATTTATTATTAGTTGGTGCACTTACTGACAATAGTGCCAAAGCCGAATCTAAGAAAGATGATACTGATTTGAAGTTAGTTAGTCATAACGTTTATATGTTATCGACCGTTTTGTATCCaaa                                                                                                                                              <  2:1161356/149‑1 (MQ=255)

       aaaaaaGTTGCAACACTTGCATTAGCAAATTTATTATTAGTTGGTGCACTTACTGACAATAGTGCCAAAGCCGAATCTAAGAAAGATGATACTGATTTGAAGTTAGTTAGTCATAACGTTTATATGTTATCGACCGTTTTGTATCCaaa                                                                                                                                              <  1:97954/149‑1 (MQ=255)

           aaGTTGCAACACTTGCATTAGCAAATTTATTATTAGTTGGTGCACTTACTGACAATAGTGCCAAAGCCGAATCTAAGAAAGATGATACTGGTTTGAAGTTAGTTAGTCATAACGTTTATATGTTATCGACCGTTTTGTATCCAAACTgg                                                                                                                                          <  1:1140066/149‑1 (MQ=255)

                  aaCACTTGCATTAGCAAATTTATTATTAGTTGGTGCACTTACTGACAATAGTGCCAAAGCCGAATCTAAGAAAGATGATACTGATTTGAAGTTAGTTAGTCATAACGTGTATATGTTATCGACCGTTTTGTATCCAAACTGGA‑GACttt                                                                                                                                  >  1:471514/1‑149 (MQ=255)

                    cacTTGCATTAGCAAATTTATTATTAGTTGGTGGACTTACTGACAATAGTGCCAAAGCCGAATCTAAGAAAGATGATACTGATTTGAAGTTAGTTAGTCATAACGTTTATATGTTATCGACCGTTTTGTATCCAAACTGGA‑GACTTTTa                                                                                                                                <  1:1168147/149‑1 (MQ=255)

                    cacTTGCATTAGCAAATTTATTATTAGTTGGTGCACTTACTGACAATAGTGCCAAAGCCGAATCTAAGAAAGATGATACTGATTTGAAGTTAGTTAGTCATAACGTTTATATGTTATCGACCGTTTTGTATCCAAACTGGA‑GACTTTTa                                                                                                                                <  1:1583102/149‑1 (MQ=255)

                    cacTTGCATTAGCAAATTTATTATTAGTTGGTGCACTTACTGACAATAGTGCCAAAGCCGAATCTAAGAAAGATGATACTGATTTGAAGTTAGTTAGTCATAACGTTTATATGTTATCGACCGTTTTGTATCCAAACTGGA‑GACTTTTa                                                                                                                                <  1:1547527/149‑1 (MQ=255)

                    cacTTGCATTAGCAAATTTATTATTAGTTGGTGCACTTACTGACAATAGTGCCAAAGCCGAATCTAAGAAAGATGATACTGATTTGAAGTTAGTTAGTCATAACGTTTATATGTTATCGACCGTTTTGTATCCAAACTGGA‑GACTTTTa                                                                                                                                <  1:468446/149‑1 (MQ=255)

                                  aaTTTATTATTAGTTGGTGCACTTACTGACAATAGTGCCAAAGCCGAATCTAAGAAAGATGATACTGATTTGAAGTTAGTTAGTCATAACGTTTATATGTTATCGACCGTTTTGTATCCAAACTGGA‑GACTTTTAACATAAAATTACtt                                                                                                                  >  2:475608/1‑149 (MQ=255)

                                   aTTTATTATTAGTTGGTGCACTTACTGACAATAGTGCCAAAGCCGAATCTAAGAAAGATGATACTGATTTGAAGTTAGTTAGTCATAACGTTTATATGTTATCGACCGTTTTGTATCCAAACTGGA‑GACTTTTAACATAAAATTACTTa                                                                                                                 <  1:673685/149‑1 (MQ=255)

                                   aTTTATTATTAGTTGGTGCACTTACTGACAATAGTGCCAAAGCCGAATCTAAGAAAGATGATACTGATTTGAAGTTAGTTAGTCATAACGTTTATATGTTATCGACCGTTTTGTATCCAAACTGGA‑GACTTTTAACATAAAATTACTTa                                                                                                                 <  2:915403/149‑1 (MQ=255)

                                        ttattaGTTGGTGCACTTACTGACAATAGTGCCAAAGCCGAATCTAAGAAAGATGATACTGATTTGAAGTTAGTTAGTCATAACGTTTATATGTTATCGACCGTTTTGTATCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCAtt                                                                                                            <  1:106314/149‑1 (MQ=255)

                                              gTTGGTGCACTTACTGACAATAGTGCCAAAGCCGAATCTAAGAAAGATGATACTGATTTGAAGTTAGTTAGTCATAACGTTTATATGTTATCGACCGTTTTGTATCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCATTCaaaaa                                                                                                      <  2:854845/149‑1 (MQ=255)

                                              gTTGGTGCACTTACTGACAATAGTGCCAAAGCCGAATCTAAGAAAGATGATACTGATTTGAAGTTAGTTAGTCATAACGTTTATATGTTATCGACCGTTTTGTATCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCATTCaaaaa                                                                                                      <  1:1273381/149‑1 (MQ=255)

                                              gTTGGTGCACTTACTGACAATAGTAAAAAGGCCGAATCTAAGAAAGCTGATACTCAATTCAAGTTAGTTAGTCATAACGTTTATATGTTATCGACCGTTTTGTATCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCATTCaaaaa                                                                                                      <  2:1559056/149‑1 (MQ=255)

                                               ttGGTGCACTTACTGACAATAGTGCCAAAGCCGAATCTAAGAAAGATGATACTGATTTGAAGTTAGTTAGTCATAACGTTTATATGTTATCGACCGTTTTGTATCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCATTCaaaaaa                                                                                                     >  2:147517/1‑148 (MQ=255)

                                               ttGGTGCACTTACTGACAATAGTGCCAAAGCCGAATCTAAGAAAGATGATACTGATTTGAAGTTAGTTAGTCATAACGTTTATATGTTATCGACCGTTTTGTATCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCATTCAAAAAg                                                                                                     <  2:526053/149‑1 (MQ=255)

                                               ttGGTGCACTTACTGACAATAGTGCCAAAGCCGAATCTAAGAAAGATGATACTGATTTGAAGTTAGTTAGTCATAACGTTTATATGTTATCGACCGTTTTGTATCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCATTCAAAAAg                                                                                                     >  1:1516384/1‑149 (MQ=255)

                                               ttGGTGCACTTACTAACAATAGTGCCAAAGCCGAATCTAAGAAAGATGATACTGATTTGAAGTTAGTTAGTCATAAAGTTTATATGTTATCGACCGTTTTGTATCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCAATCAAAAAg                                                                                                     >  2:955129/1‑149 (MQ=255)

                                                tGGTGCACTTACTGACAATAGTGCCACAGCCGAATCTAAGAAAGATGATACTGATTTGAAGTTAGTTAGTCATAACGTTTATATGTTATCGACCGTTTTGTATCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCATTCAAAAAGt                                                                                                    <  2:108168/149‑1 (MQ=255)

                                                tGGTGCACTTACTGACAATAGTGCCAAAGCCGAATCTAAGAAAGATGATACTGATTTGAAGTTAGTTAGTCATAACGTTTATATGTTATCGACCGTTTTGTATCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCATTCAAAAAGt                                                                                                    <  2:1032573/149‑1 (MQ=255)

                                                tGGTGCACTTACTGACAATAGTGCCAAAGCCGAATCTAAGAAAGATGATACTGATTTGAAGTTAGTTAGTCATAACGTTTATATGTTATCGACCGTTTTGTATCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCATTCAAAAAGt                                                                                                    <  1:446719/149‑1 (MQ=255)

                                                     cACTTACTGACAATAGTGCCAAAGCCGAATCTAAGAAAGATGATACTGATTTGAAGTTAGTTAGTCATAACGTTTATATGTTATCGACCGTTTTGTATCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCATTCAAAAAGTAAAAc                                                                                               <  1:1057894/149‑1 (MQ=255)

                                                       cTTACTGACATTAGTCCCAAAGCCGAATCTAAGAAAGATGATACTGATTTGAAGTTAGTTAGTCATAACGTTTATATGTTATCGACCGGTTTGTATCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCATTCAAAAAGTAAAACAg                                                                                             <  1:165165/149‑1 (MQ=255)

                                                       cTTACTGACAATAGTGCCAAAGCCGAATCTAAGAAAGATGATACTGATTTGAAGTTAGTTAGTCATAACGTTTATATGTTATCGACCGTTTTGTATCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCATTCAAAAAGTAAAACAg                                                                                             <  2:925994/149‑1 (MQ=255)

                                                       cTTACTGACAATAGTGCCAAAGCCGAATCTAAGAAAGATGATACTGATTTGAAGTTAGTTAGTCATAACGTTTATATGTTATCGACCGTTTTGTATCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCATTCAAAAAGTAAAACAg                                                                                             <  2:1529063/149‑1 (MQ=255)

                                                         tACTGACAATAGGGCCAAAGCCGAATCTAAGAAAGATGATACTGATTTGAAGTTAGTTAGTCATAACGTTTATATGTTATCGACCGTTTTGTATCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCATTCAAAAAGTAAAACAGCa                                                                                           <  2:243462/149‑1 (MQ=255)

                                                            tGACAATAGTGCCAAAGCCGAATCTAAGAAAGATGATACTGATTTGAAGTTAGTTAGTCATAACGTTTATATGTTATCGACCGTTTTGTATCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCATTCAAAAAGTAAAACAGCataa                                                                                        >  1:311656/1‑149 (MQ=255)

                                                            tGACAATAGTGCCAAAGCCGAATCTAAGAAAGATGATACTGATTTGAAGTTAGTTAGTCATAACGTTTATATGTTATCGACCGTTTTGTATCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCATTCAAAAAGTAAAACAGCataa                                                                                        >  2:453787/1‑149 (MQ=255)

                                                             gACATTAGTGCCAAAGCCGAATCTAAGAAAGATGATACTGATTTGAAGTTAGTTAGTCATAACGTTTATATGTTATCGACCGTTTTGTATCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCATTCAAAAAGTAAAACAGCataat                                                                                       >  2:210800/1‑149 (MQ=255)

                                                             gACAATAGTGCCAAAGCCGAATCTAAGAAAGATGATACTGATTTGAAGTTAGTTAGTCATAACGTGTATATGTTATCGACCGTTTTGTATCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCATTCAAAAAGTAAAACAGCataat                                                                                       >  2:1688506/1‑149 (MQ=255)

                                                             gACAATAGTGCCAAAGACGAATCTAAGAAAGATGATAATGATTTGAAGTTAGTTAGTAATAAAGTTTATATGTTATAGATCGTTTTGTTTCAAAAATGGA‑GACTTTTAATATAAAATTACTTATCATTCAAAAAGGAAAACATaataat                                                                                       >  2:577272/1‑149 (MQ=255)

                                                               gaaTAGTGCCAAAGCCGAATCTAAGAAAGATGATACTGATTTGAAGTTAGTTAGGCATCACGTTTATATGTTATCGACCGTTTTGTATCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCATTCAAAAAGTACAACAGCATAatat                                                                                     >  2:872170/2‑149 (MQ=255)

                                                               cAATAGTGCCAAAGCCGAATCTAAGAAAGATGATACTGATTTGAAGTTAGTTAGTCATAACGTTTATATGTTATCGACCGTTTTGTATCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCATTCAAAAAGTAAAACAGCATAatat                                                                                     >  2:1317017/1‑149 (MQ=255)

                                                                aaTAGTGCCAAAGCCGAATCTAAGAAAGATGATACTGATTTGAAGTTAGTTAGTCATAACGTTTATATGTTATCGACCGTTTTGTATCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCATTCAAAAAGTAAAACAGCATAATATc                                                                                    >  2:1428235/1‑149 (MQ=255)

                                                                    gTGCCAAAGCCGAATCTAAGAAAGATGATACTGATTTGAAGTTAGTTAGTCATAACGTTTATATGTTATCGACCGTTTTGTATCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCATTCAAAAAGTAAAACAGCATAATATCAAgg                                                                                >  2:1327800/1‑149 (MQ=255)

                                                                     tGCCAAAGCCGAATCTAAGAAAGATGCTACTAAATAGAAGTTAGTTAGTCATAACGTTTATATGTTATCGACCGTTTTGTATCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCATTCAAAAAGTAAAACAGCATAATATCAAGGt                                                                               >  1:413700/1‑149 (MQ=255)

                                                                     tGCCAAAGCCGAATCTAAGAAAGATGATACTGATTTGAAGTTAGTTAGTCATAACGTTTATATGTTATCGACCGTTTTGTATCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCATTCAACAAGTACAACAGCATATTATCAAGGt                                                                               >  2:58830/1‑149 (MQ=255)

                                                                     tGCCAAAGCCGAATCTAAGAAAGATGATACAGATTTGAAGTTAGTTAGTCATAACGTTTATATGTTATCGACCGTTTTGTATCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCATTCAAAAAGTAAAACAGCATAATATCAAGGt                                                                               >  2:1254740/1‑149 (MQ=255)

                                                                      tccAAAGCCGAATCTAAGAAAGATGATACTGATTTGAAGTTAGTTAGTCATAACGTTTATATGTTATCGACCGTTTTGTATCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCATTCAAAAAGTAAAACAGCATAATATCAAGGtt                                                                              <  1:917566/148‑1 (MQ=255)

                                                                      gtcAAAGCCGAATCTAAGAAATATGATACTGATTTGAAGTTAGTTAGTAATAACGTTTATATGTTATCGACCGTTTTGTATCCAAACTGGC‑GACTTGTAACATAAAATTACTTATCATTCAAAAAGTAAAACAGCATAATATCAAGGtt                                                                              <  2:526161/147‑1 (MQ=255)

                                                                      gcgataGCTGAATCTAAGAAAGATGATACTGTTTTGAAGTTAGTTAGTCATAACGTTTATATGTTATCGACCGTTTTGTATCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCATTCAAAAAGTAAAACAGCATAATATCAAGGtt                                                                              <  2:1620982/144‑1 (MQ=255)

                                                                      gCCAAAGCCGAATCTAAGAAAGATGCTACTGATTTTAAGTGACTTAGTCATTACGTTTATATGTTATCGACCGTTTTGTATCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCATTCAAAAAGTAAAACAGCATAATATCAAGGtt                                                                              <  1:654387/149‑1 (MQ=255)

                                                                      gCCAAAGCCGAATCTAAGAAAGATGATACTGATTTGAAGTTAGTTAGTCATAACGTTTATATGTTATCGATCGTTTTGTATCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCATTCAAAAAGTAAAACAGCATAATATCAAGGtt                                                                              <  1:606798/149‑1 (MQ=255)

                                                                      gCCAAAGCCGAATCTAAGAAAGATGATACTGATTTGAAGTTAGTTAGTCATAACGTTTATATGTTATCGACCGTTTTGTATCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCATTCAAAAAGTAAAACAGCATAATATCAAGGtt                                                                              <  2:140121/149‑1 (MQ=255)

                                                                      gCCAAAGCCGAATCTAAGAAAGATGATACTGATTTGAAGTTAGTTAGTCATAACGTTTATATGTTATCGACCGTTTTGTATCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCATTCAAAAAGTAAAACAGCATAATATCAAGGtt                                                                              >  2:1508826/1‑149 (MQ=255)

                                                                      gCCAAAGCCGAATCTAAGAAAGATGATACTGATTTGAAGTTAGTTAGTCATAACGTTTATATGTTATCGACCGTTTTGTATCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCATTCAAAAAGTAAAAAAGCATAATATCAAGGtt                                                                              >  2:754056/1‑149 (MQ=255)

                                                                       ccAAAGCCGAATCTAAGAAAGATGATACTGATTTGAAGTTAGTTAGTCATAACGTTTATATGTTATCGACCGTTTTGTATCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCATTCAAAAAGTAAAACAGCATAATATCAAGGttt                                                                             <  2:1437591/149‑1 (MQ=255)

                                                                       ccAAAGCCGAATCTAAGAAACATGATACTGATTTGAAGTTAGTTAGTCATAACGTTTATATGTTATCGACCGTTTTGTATCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCATTCAAAAAGTAAAACAGCATAATATCAAGGttt                                                                             <  1:1274217/149‑1 (MQ=255)

                                                                        cAAAGCCTCATATAAGAAAGATGATACTGATTTGAAGTTAGTTAGTCATACCGTTTATATGTTATCTACCGTTTTGTATCCAAACTGGA‑GACTTTTCACATAAAATTACTTATCATTCAGAAAGTAAAACAGCATAATATCAAGGTTta                                                                            <  2:876063/149‑1 (MQ=255)

                                                                         aaaGCCGAATCTAAGAAAGATGATACTGATTTGAAGTTAGTTAGTCATTACGTTTATATGTTATCGACCGTTTTGTATCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCATTCAAAAAGTAAAACAGCATAATATCAAGGTTtat                                                                           <  1:814967/149‑1 (MQ=255)

                                                                              cGAATCTAAGAAAGATGATACTGATTTGAAGTTAGTTAGTCATAACGTTTATATGTTATCGTCCGTTTTGTATCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCATTCAAAAAGTAAAACAGCATAATATCAAGGTTTATAACtt                                                                      <  2:807541/149‑1 (MQ=255)

                                                                               gAATCTAAGAAAGATGATACTGATTTGAAGTTAGTTAGTCATAACGTTTATATGTTATCGACCGTTTTGTATCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCATTCAAAAAGTAAAACAGCATAATATCAAGGTTTATAACttt                                                                     <  1:19532/149‑1 (MQ=255)

                                                                                 aTCTAAGAAAGATGATACTGATTTGAAGTTAGTTAGTCATAACGTTTATATGTTATCGACCGTTTTGTATCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCATTCAAAAAGTAAAACAGCATAATATCAAGGTTTATAACTttat                                                                   >  1:795767/1‑149 (MQ=255)

                                                                                 aTCTAAGAAAGATGATACTGATTTGAAGTTAGTTAGTCATAACGTTTATATGTTATCGACCGTTTTGTATCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCATTCAAAAAGTAAAACAGCATAATATCAAGGTTTATAACTttat                                                                   >  1:1405937/1‑149 (MQ=255)

                                                                                  tCTAAGAAAGATGATACTGATTTGAAGTTAGTTAGTCATAACGTTTATATGTTATCGACCGTTTTGTATCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCATTCAAAAAGTAAAACAGCATAATATCAAGGTTTATAACTttatc                                                                  >  1:1160959/1‑149 (MQ=255)

                                                                                    tAAGAAAGATGATACTGATTTGAAGTTAGTTAGTCATAACGTTTATATGTTATCGACCGTTTTGTATCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCATTCAAAAAGTAAAACAGCATAATATCAAGGTTTATAACTttatcct                                                                <  2:1309801/149‑3 (MQ=255)

                                                                                      agaaagaTGATACTGATTTGAAGTTAGTTAGTCATAACGTTTATATGTTATAGACCGTTTTGTATCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCATTCAAAAAGTAAAACAGCATAATATCAAGGTTTATAACTttatcatta                                                              >  2:1216509/1‑149 (MQ=255)

                                                                                        aaagaTGATACTGATTTGAAGTTAGTTAGTCATAACGTTTAGATGTTATCGACCGTTTTGTATCCAAACTGGA‑GACTTTTAACATAAAATCACTTATCATTCAAAAAGTAAAACAGCATAATATCAAGGTTTATAACTttatcattatc                                                            >  2:1135384/1‑149 (MQ=255)

                                                                                               atACTGATTTGAAGTTAGTTAGTCATAACGTTTATATGTTATCGACCGTTTTGTATCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCATTCAAAAAGTAAAACAGCATAATATCAAGGTTTATAACTTTATCATTATCAataata                                                     <  2:791288/149‑1 (MQ=255)

                                                                                                tACTTATTTGAAGTTAGTTAGTCATAACGTTTATATGTTATCGACCGTTTTGTATCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCATTCAAAAAGTAAAACAGCATAATATCAAGGTTTATAACTTTATCATTATCAATAATAc                                                    <  2:353474/149‑1 (MQ=255)

                                                                                                tACTGATTTGAAGTTAGTTAGTCATAACGTTTATATGTTATCGACCGTTTTGTATCCAAACTGGA‑GACTTTTAACATAAAAATACTCATGATTCAAAAAGTAAAACAGCATAATTTCAAGGTTTATAACTTTATCATTATCAATAATAc                                                    >  1:400531/1‑149 (MQ=255)

                                                                                                 aagtaTTTGAAGTTAGTTAGTCAGAAGGTTTATATGTTATCCACCGTTTTGGATCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCATTCAAAAAGGAAAACAGCATAATATCAAGGTTTATAACTTTATCATTATCAATAATAcc                                                   <  1:1661180/145‑1 (MQ=255)

                                                                                                  cTGATTTGAAGTTAGTTAGTCATAACGTTTATATGTTATCGACCGTTTTGTATCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCATTCAAAAAGTAAAACAGCATAATATCAAGGTTTATAACTTTATCATTATCAATAATACCt                                                  <  1:1453087/149‑1 (MQ=255)

                                                                                                      tttGAAGTTAGTTAGTCATAACGTTTATATGTTATCGACCGTTTTGTATCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCATTCAAAAAGTAAAACAGCATAATATCAAGGTTTATAACTTTATCATTATCAATAATACCTCata                                              >  2:1595381/1‑149 (MQ=255)

                                                                                                      tttGAAGTTAGTTAGTCATAACGTTTATATGTTATCGACCGTTTTGTATCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCATTCAAAAAGTAAAACAGCATAATATCAAGGTTTATAACTTTATCATTATCAATAATACCTCata                                              >  1:600969/1‑149 (MQ=255)

                                                                                                         gAAGTTAGTTAGTCATAACGTTTATATGTTATCGACCGTTTTGTATCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCATTCAAAAAGTAAAACAGCATAATATCAAGGTTTATAACTTTATCATTATCAATAATACCTCATataa                                           <  1:1391088/149‑1 (MQ=255)

                                                                                                                     tCATAACGTTTATATGTTATCGACCGTTTTGTATCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCATTCAAAAAGTAAAACAGCATAATATCAAGGTTTATAACTTTATCATTATCAATAATACCTCATATAAACTAAAAtttta                               >  2:282247/1‑149 (MQ=255)

                                                                                                                     tCATAACGTTTATATGTTATCGACCGTTTTGTATCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCATTCAAAAAGTAAAACAGCATAATATCAAGGTTTATAACTTTATCATTATCAATAATACCTCATATAAAATAAAAtttta                               >  2:239520/1‑149 (MQ=255)

                                                                                                                      cATAACGTTTATATGTTATCGACCGTTTTGTATCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCATTCAAAAAGTAAAACAGCATAATATCAAGGTTTATAACTTTATCATTATCAATAATACCTCATATAAAATAAAAttttag                              <  1:393442/149‑1 (MQ=255)

                                                                                                                      cATAACGTTTATATGTTATCGACCGTTTTGTATCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCATTCAAAAAGTAAAACAGCATAATATCAAGGTTTATAACTTTATCATTATCAATAATACCTCATATAAAATAAAAttttag                              >  2:1146645/1‑149 (MQ=255)

                                                                                                                      cATAACGTTTATATGTTATCGACCGTTTTGTATCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCATTCAAAAAGTAAAACAGCATAATATCAAGGTTTATAACTTTATCATTATCAATAATACCTCATATAAAATAAAAttttag                              >  1:1698052/1‑149 (MQ=255)

                                                                                                                      cATAACGTTTATATGTTATCGACCGTTTTGTATCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCATTCAAAAAGTAAAACAGCATAATATCAAGGTTTATAACTTTATCATTATCAATAATACCTCATATAAAATAAAAtattag                              >  1:455666/1‑149 (MQ=255)

                                                                                                                            gTTTATATGTTATCGACCGTTTTGTATCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCATTCAAAAAGTAAAACAGCATAATATCAAGGTTTATAACTTTATCATTATCAATAATACCTCATATAAAATAAAAttttagggactt                        >  1:1336696/1‑149 (MQ=255)

                                                                                                                            gTTTATATGTTATCGACCGTTTTATATCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCATTCAAAAAGTAAAACAGCATAATATCAAGGTTTATAACTTTATCATTATCAATAATACCTCATATAAAATAAAAttttagggactt                        >  1:881854/1‑149 (MQ=255)

                                                                                                                               tataTGTTATCGACCGTTTTGTATCCAAATTGGC‑GACTTTTAACATAAAATTACTTATCATTCAAAAAGTAAAACAGCATAATATCAAGGTTTGTAACTTTTTCCTTATCAATAATACCTCATATAAACATAAAttttaggtacgtttt                     >  1:263454/1‑149 (MQ=255)

                                                                                                                               tataTGTTATCGACCGTTTTGTAGCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCATTCAAAAAGTAAAACAGCATAATATCAAGGTTTATAACTTTATAATTATCAATAATACCTCATATAAAATAAAAttttagggacttttt                     <  2:297065/149‑1 (MQ=255)

                                                                                                                                   tGTTATCGACCGTTTTGTATCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCATTCAAAAAGTAAAACAGCATAATATCAAGGTTTATAACTTTATCATTATCAATAATACCTCATATAAAATAAAAttttagggactttttaggg                 >  1:485011/1‑149 (MQ=255)

                                                                                                                                    gTTATCGACCGTTTTGTATCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCATTCAAAAAGTAAAACAGCATAATATCAAGGTTTATAACTTTATCATTATCAATAATACCTCATATAAAATAAAAttttagggactttttaggga                >  1:1425043/1‑149 (MQ=255)

                                                                                                                                    gTTATCGACCGTTTTGTATCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCATTCAAAAAGTAAAACAGCATAATATCAAGGTTTATAACTTTATCATTATCAATAATACCTCATATAAAATAAAAttttagggactttttaggga                >  1:1346122/1‑149 (MQ=255)

                                                                                                                                    gTTATCGACCGTTTTGTATCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCATTCAAAAAGTAAAACAGCATAATATCAAGGTTTATAACTTTATCATTATCAATAATACCTCATATAAAATAAAAttttagggactttttaggga                >  1:1578067/1‑149 (MQ=255)

                                                                                                                                     ttATCGACCGTTTTGTATCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCATTCAAAAAGTAAAACAGCATAATATCAAGGTTTATAACTTTATCATTATCAATAATACCTCATATAAAATAAAAttttagggcctttttagggac               >  1:72699/1‑149 (MQ=255)

                                                                                                                                     ttATCGACCGTTTTGTATCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCATTCAAAAAGTAAAACAGCATAATATCAAGGTTTATAACTTTATCATTATCAATAATACCTCATATAAAATAAAAtttgagggaatttttagggaa               >  1:123074/1‑148 (MQ=255)

                                                                                                                                     ttATCGACCGTGTTGTATCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCATTCAAAAAGTAAAACAGCATAATATCAAGGTTTATAACTTTATCATTATCAATAATACCTCATATAAAATAAAAttttagggactttttagggac               >  2:1228458/1‑149 (MQ=255)

                                                                                                                                      tATCGACCGTTTTGTATCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCATTCAAAAAGTAAAACAGCATAATATCAAGGTTTATAACTTTATCATTATCAATAATACCTCATATAAAATAAAAttttagggactttttagggact              <  1:891905/149‑1 (MQ=255)

                                                                                                                                            ccGTTTTGTATCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCATTCAAAAAGTAAAACAGCATAATATCAAGGTTTATAACTTTATCATTATCAATAATACCTCATATAAAATAAAATTTTAGGGACTTTTTAGGGACtttaaat        >  1:77051/1‑149 (MQ=255)

                                                                                                                                            ccGTTTTATATCCAAACTGGA‑GACTTTTAACACAAAATTACTTATCATTCAAAAAGTAAAACAGCATAATATCAAGGTTTATAACTTTATCATTATCAATAATACCTCATATAAAATAAAATTTTAGGGACTTTTTAGGGACtttaaat        >  1:1254554/1‑149 (MQ=255)

                                                                                                                                             cGTTTTGTATCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCATTCAAAAAGTAAAACAGCATAATATCAAGGTTTATAACTTTATCATTATCAATAATACCTCATATAAAATAAAATTTTAGGGACTTTTTAGGGACtttaaatt       <  2:837234/149‑1 (MQ=255)

                                                                                                                                             cGTTGTGTAGCCAAAGTGGACGACTTTT‑TGATGAAATTACTTATCATGCAAAAAGTAAAACAGCATAATATCGAGGTTTAGAACTTTATCATTATCAATAATACCTCATATAAAATAAAATTTTAGGGACTTTTTATGGACtttaaatt       <  2:334601/149‑1 (MQ=255)

                                                                                                                                              gTTTTGTATCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCATTCAAAAAGTAAAACAGCATAATATCAAGGTTTATAACTTTATCATTATCAATAATACCTCATATAAAATAAAATTTTAGGGACTTTTTAGGGACtttaaattt      <  2:1372425/149‑1 (MQ=255)

                                                                                                                                                 ttGTATCCAAACGGGA‑GACTTTTAACATAAAATTACTTATCACTCAAAAAGTAAAACAGCATAATATCAAGGTTTATAATTTTATCATTATCAATAATACCTCATATAAAATAAAATGTTAGGGACTTTTTAGGGACtttaaatttaaa   <  2:72358/149‑1 (MQ=255)

                                                                                                                                                  tGTATCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCATTCAAAAAGTAAAACAGCATAATATCAAGGTTTATAACTTTATCATTATCAATAATACCTCATATAAAATAAAATCTTAGGGACTTTTTAGTGACTTTAAATTTaaaa  >  2:1663592/1‑149 (MQ=255)

TTCACTAAAAAAAGTTGCAACACTTGCATTAGCAAATTTATTATTAGTTGGTGCACTTACTGACAATAGTGCCAAAGCCGAATCTAAGAAAGATGATACTGATTTGAAGTTAGTTAGTCATAACGTTTATATGTTATCGACCGTTTTGTATCCAAACTGGA‑GACTTTTAACATAAAATTACTTATCATTCAAAAAGTAAAACAGCATAATATCAAGGTTTATAACTTTATCATTATCAATAATACCTCATATAAAATAAAATTTTAGGGACTTTTTAGGGACTTTAAATTTAAAA  >  USA300TCH1516_ALE/2085150‑2085444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.