BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	1,416,458	A→T	I216N (ATT→AAT)	oppD_4 ←	Oligopeptide transport ATP‑binding protein OppD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,416,458	0	A	T	100.0%	372.6 / NA	111	I216N (ATT→AAT)	oppD_4	Oligopeptide transport ATP‑binding protein OppD
Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (61/50); total (61/50)

Only 100 of 111 total aligned reads displayed.

AGTCACATGTTTTAACTCAATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTG‑AAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAAATGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACTGGTTCGTCTGC  >  USA300TCH1516_ALE/1416312‑1416600

aGTCACATGTTTTAACTCAATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTG‑AAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTg                                                                                                                                              <  1:1715494/149‑1 (MQ=255)

           ttAACTCAATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTG‑AAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAata                                                                                                                                   <  1:1398444/149‑1 (MQ=255)

             aaCTCATTCATAGTAATCCCTCTTTAATTGTGTCCTATATTTAATTAGTCGTTCAGTATACGGATGCAAATGCTCAGACTTG‑AGATGACTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATAtt                                                                                                                                 <  2:1410499/149‑1 (MQ=255)

             aaCTCAATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGCCTGTCGGCGATCTGACGTGCTCACGA‑GATTATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATAtt                                                                                                                                 <  1:828609/149‑1 (MQ=255)

              aCTCAAGCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTG‑AAATGATTAATGTTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATAttt                                                                                                                                <  1:258601/149‑1 (MQ=255)

                 cAATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTG‑AAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCaa                                                                                                                             >  2:645473/1‑149 (MQ=255)

                 cAATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTG‑AAATAGTTGATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCaa                                                                                                                             >  2:1039051/1‑149 (MQ=255)

                       tAGTAATCCCTCTTTAATTGTGTTCTATATTTGATTGGACGTTCCGTCAACATGTGGAAATGCTCATACTTG‑AAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTTACAATATTTCAATACATg                                                                                                                       <  1:1245150/149‑1 (MQ=255)

                       tAGTAATCCCGCTTTAATTGTGTTCTATATTTAATTAGCCGTTCAGTATACGGATGCAAATGCTCATACTTG‑AAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATg                                                                                                                       <  2:1375532/149‑1 (MQ=255)

                          tAACCCCTCTTTAATTGTTTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTG‑AAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACt                                                                                                                    <  1:204133/149‑1 (MQ=255)

                            aTCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTCCCAGGTCGCTCATGTTCGTGCGTT‑AAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTa                                                                                                                  <  2:1212670/149‑1 (MQ=255)

                            aTCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTG‑AAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTa                                                                                                                  <  2:839905/149‑1 (MQ=255)

                            aTCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTG‑AAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTa                                                                                                                  <  2:21819/149‑1 (MQ=255)

                            aTCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTG‑AAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTa                                                                                                                  <  1:511269/149‑1 (MQ=255)

                             tCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTG‑AAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTAAATCCATGACTTaa                                                                                                                 >  1:428014/1‑149 (MQ=255)

                              cccTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTG‑AAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAg                                                                                                                >  2:53760/1‑149 (MQ=255)

                               ccTCTTTAATTGTGTTGTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCAAACTTG‑AAATGATTAATATTACCTCGTTCAATGATTGGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGt                                                                                                               >  2:1270257/1‑149 (MQ=255)

                               ccTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTG‑AAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATATATGACTTAAGt                                                                                                               >  2:1209012/1‑149 (MQ=255)

                               ccTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTG‑AAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGt                                                                                                               >  2:382352/1‑149 (MQ=255)

                                  ctTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTG‑AAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTAt                                                                                                            <  1:298799/149‑1 (MQ=255)

                                  ctTTAATTGTGTGCTATATTTAATTAGACGTTCAGTATATGGATGCAAATGCTCATACTTG‑AAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTAt                                                                                                            <  1:1354069/149‑1 (MQ=255)

                                       aTTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTG‑AAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTAtgtgtg                                                                                                       <  1:1416722/149‑1 (MQ=255)

                                       aTTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTG‑AAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTAtgtgtg                                                                                                       <  1:365607/149‑1 (MQ=255)

                                        ttGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTG‑AAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGa                                                                                                      <  2:111974/149‑1 (MQ=255)

                                        ttGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTG‑AAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGa                                                                                                      <  1:199089/149‑1 (MQ=255)

                                        ttGTGTTCTAGATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTG‑AAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGa                                                                                                      <  1:1142625/149‑1 (MQ=255)

                                           tgtTTTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTG‑AAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGataa                                                                                                   <  2:466417/149‑1 (MQ=255)

                                           tgtTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTG‑AAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACACTATTTCAATACATGACTTAAGTTATGTGTGataa                                                                                                   >  2:403086/1‑149 (MQ=255)

                                            gtTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTG‑AAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGataat                                                                                                  >  1:457072/1‑149 (MQ=255)

                                              tCTATATTTAATTAGACGTTCAGTATACGGATTCAAATGTTCATACTTG‑AAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGAtaataa                                                                                                <  1:744148/149‑1 (MQ=255)

                                              tCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTG‑AAATGATTAATATAAGCTCGTGCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGAtaataa                                                                                                <  1:1689513/149‑1 (MQ=255)

                                               cTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTG‑AAATGATTAATATTACCTCGTTCAATGAGTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATaataaa                                                                                               <  2:233810/149‑1 (MQ=255)

                                                tataTTTAATTAGACGTTCAGTATACGGATGCACCTGCTCATACTTG‑AAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATaataaat                                                                                              >  2:871061/1‑149 (MQ=255)

                                                tataTTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTG‑AAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATaataaat                                                                                              >  1:1017495/1‑149 (MQ=255)

                                                 atatTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTG‑AAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATaataaata                                                                                             <  1:805778/149‑1 (MQ=255)

                                                    tttAATTAGACGTTCAGTATACGGATGCACATGCTCATACTTG‑GAATGATTAATATTACCTCGTTCACTGATTTGACCTTCTTTTACAACATAATAGTACTGACACTATTTCAATACATGACGTAAGTTATGTGTGATaataaaatatg                                                                                          >  2:1467984/1‑144 (MQ=255)

                                                     ttAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTG‑AAATGGTTAATATTAACTCGTTCAATGATTTGACCTTCTTTTAAAACATAGTTGTAATGAAAAAATTCCAATACATGAATTAAATTAGGTTTGATAATAaataatat                                                                                         >  2:361488/1‑147 (MQ=255)

                                                     ttAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTG‑AAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGt                                                                                         >  2:533679/1‑149 (MQ=255)

                                                     ttAATTAGACGGTCAGTATACGGATGCAAATACTCATACTTG‑AAATGATTAATATTACCTCGTTCAATGATTTGTCCTTCTTTTAAAACATAATTGTACTGACAATATTTCCATACATGACTTAAGTTTTAGGTGATaataaatatcat                                                                                         >  2:1716636/1‑145 (MQ=255)

                                                        aTTAGACGTTCAGTATACGGATGCAAATGCTCATACTTG‑AAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTg                                                                                      <  1:469541/149‑1 (MQ=255)

                                                          tAGACGTTCAGTATACGGATGCAAATGCTCATACTTG‑AAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGAc                                                                                    <  1:75057/149‑1 (MQ=255)

                                                          tAGACGTTCAGAATACGGATTCAAATGCTCATACTTG‑AAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGAc                                                                                    <  2:970656/149‑1 (MQ=255)

                                                            gACGTTCAGTATACGGATGCAAATGCTCATACTTG‑AAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCa                                                                                  >  1:1499354/1‑149 (MQ=255)

                                                             aCGTTCAGTATACGGATGCAAATGCTCATACTTG‑AAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCAt                                                                                 >  2:221651/1‑149 (MQ=255)

                                                                tttaGTATACGGATGCTACTGCTCATACTTG‑AAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGtt                                                                              <  2:1617696/146‑1 (MQ=255)

                                                                ttCAGTATACGGATGCAAATGCTCATACTTG‑AAATGATTAATATTACCTCGTTCAATGCTTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGtt                                                                              >  1:1389829/1‑149 (MQ=255)

                                                                ttCAGTATACGGATGCAAATGCTCATACTTG‑AAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGtt                                                                              <  2:1564882/149‑1 (MQ=255)

                                                                 tCAGTATACGGATGCAAATGCTCATACTTG‑AAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTc                                                                             >  1:1518515/1‑149 (MQ=255)

                                                                   aGTATACGGATGCAAATGCTCATACTTG‑AAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAATTTATGTGTGATAATAAATAATGTTTGACCATGTTCTa                                                                           <  2:480420/149‑1 (MQ=255)

                                                                   aGTATACGGATGCAAATGCTCATACTTG‑AAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTa                                                                           <  2:215793/149‑1 (MQ=255)

                                                                     tataCGGATGCAAATGCTCATACTTG‑AAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAAt                                                                         >  2:192578/1‑149 (MQ=255)

                                                                         cGGATGCAAATGCTCATACTTG‑AAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACaa                                                                     <  2:605353/149‑1 (MQ=255)

                                                                         cGGATGCAAATGCTCATACTTG‑AAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACaa                                                                     >  1:589358/1‑149 (MQ=255)

                                                                         aggATGCAAATGCTCATTCTTG‑AAATGATTAATAATGCCTAGTTCGACGCTTTCCGCGTCGTGGAGAAGATAATTGTACTGACAATATGTCAATACAGGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACaa                                                                     <  2:1213282/148‑1 (MQ=255)

                                                                          ggATGCAAATGCTCATACTTG‑AAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATTATAAATAATGTTTGACCATGTTCTCATACAat                                                                    >  1:63637/1‑149 (MQ=255)

                                                                          ggATGCAAATGCTCATACTTG‑AAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAat                                                                    >  1:1101124/1‑149 (MQ=255)

                                                                          ggATGCAAATGCTCATACTTG‑AAATGATTAATATTACCTCGTTCAATGATTTAACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAat                                                                    >  1:943547/1‑149 (MQ=255)

                                                                          ggATGCAAATGCGCATACTTG‑AAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTTACAATGTTCTAATACAat                                                                    >  2:1193554/1‑149 (MQ=255)

                                                                            aTGCAAATGCTCATACTTG‑AAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAatat                                                                  >  1:913715/1‑149 (MQ=255)

                                                                                 aaTGCTCATACTTG‑AAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGCCCATGTTCTAATACAATATGCTGt                                                             >  1:114932/1‑149 (MQ=255)

                                                                                 aaTGCTCATACTTG‑AAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGt                                                             >  1:1100850/1‑149 (MQ=255)

                                                                                 aaTGCTCATACTTG‑AAATGATTAATATTACCTCGTTCAATGAATTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGt                                                             >  1:913115/1‑149 (MQ=255)

                                                                                       cATACTTG‑AAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGGAATaaa                                                       >  1:1148750/1‑149 (MQ=255)

                                                                                              g‑AAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCa                                                >  1:1197938/1‑149 (MQ=255)

                                                                                                 aaTGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACt                                              >  1:1307251/1‑149 (MQ=255)

                                                                                                 aaTGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACt                                              <  1:266386/149‑1 (MQ=255)

                                                                                                       tAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTa                                        >  2:1697322/1‑149 (MQ=255)

                                                                                                         atatTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATACCTTGATTAcc                                      >  2:500442/1‑149 (MQ=255)

                                                                                                         atatTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAAAATGCTGTAATAAATCAATCACTTGATTAcc                                      >  2:1389257/1‑149 (MQ=255)

                                                                                                         atatTACCTCGTTAAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTAAATACATATGTTAAGTTAGGTGAGATAATAAATGATGTTTGACAATGGTCTAATAAAATATACTGTAATAAATACATCATTTGATTTcc                                      >  2:563973/1‑149 (MQ=255)

                                                                                                           atTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGt                                    <  1:1312868/149‑1 (MQ=255)

                                                                                                             tACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTGAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTAcgtttt                                  >  2:43304/1‑144 (MQ=255)

                                                                                                             tACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTc                                  >  2:1691577/1‑149 (MQ=255)

                                                                                                               ccTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAGGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCCCTTGTTTACCGTTCaa                                >  1:188446/1‑149 (MQ=255)

                                                                                                               ccTCGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCTTTCaa                                >  1:573268/1‑149 (MQ=255)

                                                                                                                  cGTTCAATGATTTGACCTTCTTTTTAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGc                             >  1:858523/1‑149 (MQ=255)

                                                                                                                  cGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACACTATTTCAATACCTGACTTAAGTTATGTGTGATAATAAATAATGATTGACCATGTGCTAATACAATATACTGTCATAAATCCATCACTTGATAACAGTTAAAAGc                             >  2:201209/1‑149 (MQ=255)

                                                                                                                  cGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCACTACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATAACTTGATTACCGTTCaacac                             >  1:304369/1‑146 (MQ=255)

                                                                                                                  cGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTAAGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGTTTACCGTTCAAAGc                             >  2:441559/1‑149 (MQ=255)

                                                                                                                  cGTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGAATTAAGTTATGTGTGATAATAAATAATGTTTAACCATGTTCTAATACGATATGCTGTAATAAATCTATCACTTGATTACCATTCaataa                             >  2:289943/1‑146 (MQ=255)

                                                                                                                  agTTCAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGc                             >  2:1142002/2‑149 (MQ=255)

                                                                                                                      cAATGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATcc                         <  2:1693464/149‑1 (MQ=255)

                                                                                                                      cAATGATTTGACCTTCTTTGAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATcc                         <  2:1322830/149‑1 (MQ=255)

                                                                                                                        aTGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCaa                       >  2:587876/1‑149 (MQ=255)

                                                                                                                        aTGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCaa                       <  1:1141081/149‑1 (MQ=255)

                                                                                                                        aTGATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCaa                       >  1:187262/1‑149 (MQ=255)

                                                                                                                          gATTTGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTTATTACCGTTCAAAGCATCCAatg                     <  1:871061/149‑1 (MQ=255)

                                                                                                                            tttGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGTCCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAatgat                   <  1:1039051/149‑1 (MQ=255)

                                                                                                                            tttGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAatgat                   <  1:645473/149‑1 (MQ=255)

                                                                                                                            tttGACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAatgat                   <  1:1617698/149‑1 (MQ=255)

                                                                                                                               gACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCCCTTGATTACCGTTCAAAGCATCCAATGATGCa                >  2:582548/1‑149 (MQ=255)

                                                                                                                               gACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCa                >  1:1015634/1‑149 (MQ=255)

                                                                                                                               gACCTTCTTTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCa                >  2:1146715/1‑149 (MQ=255)

                                                                                                                                 ccTTCTTTTAAAACATTATTGGCCTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAAc              <  2:1515837/149‑1 (MQ=255)

                                                                                                                                    tcttTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACTgg           >  1:1149385/1‑149 (MQ=255)

                                                                                                                                     cttTTAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCAATTGCTTACGGTTCAAAGCATCCAATGATGCACCTGGt          >  1:130386/1‑149 (MQ=255)

                                                                                                                                       tttAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACTGGTTc        >  2:1505236/1‑149 (MQ=255)

                                                                                                                                       tttAAAACATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACTGAttt        >  2:598838/1‑148 (MQ=255)

                                                                                                                                            aaCATAATTGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAGTGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCACCTGGTTCGTCTg   >  1:334323/1‑149 (MQ=255)

                                                                                                                                             aCATAATTGGACTGACAATATTTCAATACATGACTTAAGTTATGTGTTATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACTGGTTCGTCTGc  <  2:1599280/149‑1 (MQ=255)

AGTCACATGTTTTAACTCAATCATAGTAATCCCTCTTTAATTGTGTTCTATATTTAATTAGACGTTCAGTATACGGATGCAAATGCTCATACTTG‑AAATGATTAATATTACCTCGTTCAATGATTTGACCTTCTTTTAAAACATAAATGTACTGACAATATTTCAATACATGACTTAAGTTATGTGTGATAATAAATAATGTTTGACCATGTTCTAATACAATATGCTGTAATAAATCCATCACTTGATTACCGTTCAAAGCATCCAATGATGCAACTGGTTCGTCTGC  >  USA300TCH1516_ALE/1416312‑1416600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`