Predicted mutation
evidence seq id position mutation annotation gene description
RA USA300TCH1516_ALE 1,481,355 G→T A6890E (GCA→GAA)  ebh_1 ← Extracellular matrix‑binding protein ebh

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,481,3550GT98.6% 431.9 / ‑2.7 140A6890E (GCA→GAA) ebh_1Extracellular matrix‑binding protein ebh
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base T (66/70);  total (69/71)
Fisher's exact test for biased strand distribution p-value = 2.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.25e-01

Only 100 of 146 total aligned reads displayed.

AAGTTACCCATCGCTGTATTCAGTTCCTGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTGCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATTAATAATATTTTCAGCAGCAGTTACCGC  >  USA300TCH1516_ALE/1481211‑1481500
                                                                                                                                                 |                                                                                                                                                 
aaGTTGCCCATGGCTGTATTCAGTTCCTGGGCGGTTTGAATGGCTTGCGTTACTTCAGCCACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCAtt                                                                                                                                               <  2:460631/149‑1 (MQ=255)
aaGTTACCCATCGCTGTATTCAGTTCCTGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCAtt                                                                                                                                               <  2:354966/149‑1 (MQ=255)
   ttACCCATCGCTGTATTCAGTTCCTGAGCGGATTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGAAGTTACCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGtt                                                                                                                                            >  2:224478/1‑149 (MQ=255)
     aCCCATCGCTGTATTCAGTTCCTGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTCTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGtttt                                                                                                                                          <  2:864215/149‑1 (MQ=255)
        cATCGCTGTATTCAGTTCCTGAGCGGTTAGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGCGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTGTTac                                                                                                                                       >  1:1042999/1‑148 (MQ=255)
         aTCGCTGTATTCAGTTCCTGAGAGGTTTGAATGGCTTGCGTTACTTCAGCTAGAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGc                                                                                                                                      >  1:942812/1‑149 (MQ=255)
            gCTGTATTCAGTTCCTGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGGTTTTAGCATc                                                                                                                                   >  1:1663942/1‑149 (MQ=255)
            gCTGTATGCAGTTCCTGAGCGGTTTTAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTACCAGCACCTTTTTGTTCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATc                                                                                                                                   <  2:1360219/149‑1 (MQ=255)
            ccTGTATGCAGTTCCTTAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATc                                                                                                                                   <  2:783729/148‑1 (MQ=255)
              tGTTTTCAGTTCTTTAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTg                                                                                                                                 <  2:1547753/149‑1 (MQ=255)
              tGTATTCAGTTCCTGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCCTTGTTTTTAGCATATg                                                                                                                                 >  2:201714/1‑149 (MQ=255)
                tcttCAGTTCCTGGGCGGTTTGAATGGCTTGCGTCACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGtt                                                                                                                               <  2:1372491/147‑1 (MQ=255)
                tATTCAGTTCCCGGGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGtt                                                                                                                               <  1:597632/149‑1 (MQ=255)
                   tCAGTTCCTGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACt                                                                                                                            >  2:483525/1‑149 (MQ=255)
                      gTTCCTGGGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACtttt                                                                                                                         <  2:1356304/149‑1 (MQ=255)
                      gTTCCTGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACtttt                                                                                                                         <  1:273651/149‑1 (MQ=255)
                      gTTCCTGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACtttt                                                                                                                         >  1:613732/1‑149 (MQ=255)
                       ttCCTGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTc                                                                                                                        <  2:81738/149‑1 (MQ=255)
                                ggTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTTTCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCAt                                                                                                               <  2:276550/149‑1 (MQ=255)
                                ggTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCAt                                                                                                               <  1:1697230/149‑1 (MQ=255)
                                ggTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCAt                                                                                                               <  2:463426/149‑1 (MQ=255)
                                ggTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCAt                                                                                                               <  1:664239/149‑1 (MQ=255)
                                  tttGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTc                                                                                                             >  1:312766/1‑149 (MQ=255)
                                  tttGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTc                                                                                                             >  1:1358804/1‑149 (MQ=255)
                                  tttGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATGGATGTTTCCAGTAACAGCACCGTTTTGTGCATTGTTTAAGTTATCTAACCCAGATAAGGTTTGGTTTTCATTGTTTTTAGCATCTGTTTCTTTTCTATGACCAttg                                                                                                             >  1:722829/1‑148 (MQ=255)
                                   ttGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCa                                                                                                            >  1:699905/1‑149 (MQ=255)
                                      actGGCTTGCGTTACTTCAGCTACAGTGTGCGCTGTACTGATTTTTCCAGTAACAGCACCTTGTTGTGCATTGGTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACg                                                                                                         <  2:1183098/147‑1 (MQ=255)
                                        tGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCt                                                                                                       >  2:1531126/1‑149 (MQ=255)
                                             tGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGttt                                                                                                  >  1:1596881/1‑149 (MQ=255)
                                              tcGTTACTTCAGCTACAGTGTGCGCTTTATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTc                                                                                                 <  2:339905/148‑1 (MQ=255)
                                              gCGTTCCTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTc                                                                                                 <  1:549302/149‑1 (MQ=255)
                                              gCGTTCCTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTc                                                                                                 <  2:140842/149‑1 (MQ=255)
                                               ccttccTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCCCCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCg                                                                                                <  2:611974/144‑1 (MQ=255)
                                               cGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTTTTTTTTTTTTCTTTTCTATCACCATTCAACGCTTGTTTCg                                                                                                >  2:126225/1‑149 (MQ=255)
                                                  tACTTCAGTTACAGTGTGCGCTTGATTGATGTTTCCAGTACCTGCACCTTTTTGTGCATTTTTTTAGTTTATTCACTTAGGTACTCGCGCCGTGTCTTGGTGTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAg                                                                                             <  1:405708/149‑1 (MQ=255)
                                                  tACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAg                                                                                             <  1:1530973/149‑1 (MQ=255)
                                                     ttCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTGCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAgttg                                                                                          >  1:268854/1‑149 (MQ=255)
                                                     ttCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTGCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAgttg                                                                                          >  1:1543341/1‑149 (MQ=255)
                                                      tCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAgttgt                                                                                         >  1:759309/1‑149 (MQ=255)
                                                      tCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGAAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAgttgt                                                                                         >  2:687937/1‑149 (MQ=255)
                                                         gCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTAc                                                                                      >  1:1366976/1‑149 (MQ=255)
                                                             cAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGtt                                                                                  >  2:1519636/1‑149 (MQ=255)
                                                              aGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTg                                                                                 >  2:889443/1‑149 (MQ=255)
                                                                     gcTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCGTTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTGTTTTCGTATTTGTTACAGTTGAAAGtgt                                                                          >  2:1352881/1‑148 (MQ=255)
                                                                     gcTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTAGTAGTTGTTACAGTGTAAAGtac                                                                          >  2:303919/1‑147 (MQ=255)
                                                                       ttgattgaTGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGtgctg                                                                        <  1:1062889/149‑1 (MQ=255)
                                                                       ttgattgaTGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGtgctg                                                                        <  2:1216927/149‑1 (MQ=255)
                                                                         gattgatGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTgctgct                                                                      >  1:437122/1‑149 (MQ=255)
                                                                            tgatGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCa                                                                   <  1:1713788/149‑1 (MQ=255)
                                                                              atGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGCTAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCCCCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGACAGTGCTGCTTCAAc                                                                 >  1:1426816/1‑149 (MQ=255)
                                                                               tGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACt                                                                >  1:1223490/1‑149 (MQ=255)
                                                                               tGTTTCCAGTAAAAGCACCTTTTTGTGCATTGTTTAAGTTCTCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACt                                                                >  1:1232471/1‑149 (MQ=255)
                                                                                gTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATTTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACtt                                                               <  2:291739/149‑1 (MQ=255)
                                                                                     cAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGAt                                                          >  1:1648050/1‑149 (MQ=255)
                                                                                      aGTAACAGCGCCTTTTCGTTCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCCTTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGAtt                                                         <  1:850117/149‑1 (MQ=255)
                                                                                          aCAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTTCACTTTTGATTGGtt                                                     >  2:1663136/1‑149 (MQ=255)
                                                                                          aCAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGTTTGGtt                                                     >  1:330742/1‑149 (MQ=255)
                                                                                             gCATCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGc                                                  <  1:782427/149‑1 (MQ=255)
                                                                                             gCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCCTTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGc                                                  <  2:1433107/149‑1 (MQ=255)
                                                                                             gCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGc                                                  <  1:1160022/149‑1 (MQ=255)
                                                                                                 cTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCAttt                                              >  1:630179/1‑149 (MQ=255)
                                                                                                 cTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCAttt                                              >  1:225544/1‑149 (MQ=255)
                                                                                                  tttttGTGCATTGTTTAAGTTATCTAACGTCGGTTGATATTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTg                                             >  1:1703892/1‑149 (MQ=255)
                                                                                                  tttttGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTg                                             <  2:1015074/149‑1 (MQ=255)
                                                                                                  tttttGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTg                                             >  2:1562774/1‑149 (MQ=255)
                                                                                                    tttGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTa                                           <  2:263169/149‑1 (MQ=255)
                                                                                                      tgtgCATTTTTTAAGTTTTCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCCCCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTAcc                                         <  1:680263/149‑1 (MQ=255)
                                                                                                      tgtgCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTAcc                                         <  1:100883/149‑1 (MQ=255)
                                                                                                      tgtgCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTAcc                                         <  1:551090/149‑1 (MQ=255)
                                                                                                      tgtgCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGCTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTAcc                                         <  1:486134/149‑1 (MQ=255)
                                                                                                          cATTGTTTAAGTTATCTAACGTAGTTAAGTGTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCAttt                                     <  2:1466607/149‑1 (MQ=255)
                                                                                                          cATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACTGTTGAAAGTGCTGCTTCAACTTCTGATTGGTTCGCATTTGTACCAttt                                     >  2:1054622/1‑149 (MQ=255)
                                                                                                              gTTTAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCtt                                 <  1:1383844/149‑1 (MQ=255)
                                                                                                                ttACGTTACCTAACGTAGATAATGTTTTGTTTTCATTGTTTTTAGCATCGGTTACTTTTCTATCACCATTCAACGCTTTTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGa                               <  2:235217/149‑1 (MQ=255)
                                                                                                                ttAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGa                               <  1:1088718/149‑1 (MQ=255)
                                                                                                                ttAAGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGa                               <  1:224478/149‑1 (MQ=255)
                                                                                                                 tAAGTTATCTAGCGTATATATTGTTTGGTTTTCATTGTTTTTTGCATCTGTTACTTTTCTATCACCATTCAATGCTTGTTTCGTTGTTGTTACAGTTGAAAGTGTTGCTGCAGGTTGGGGTTGGTTCGCTTTTGTACCATTTGCTTGAt                              <  2:717573/149‑1 (MQ=255)
                                                                                                                   atttAGCTAACGTAGTTAAGTTTTGGTTCTCATTGTGTTTTGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATta                            <  2:36455/147‑1 (MQ=255)
                                                                                                                   aGTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATta                            <  1:483525/149‑1 (MQ=255)
                                                                                                                    gTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATtaa                           >  2:323094/1‑149 (MQ=255)
                                                                                                                    gTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATtaa                           >  1:1525837/1‑149 (MQ=255)
                                                                                                                    gTTATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATtaa                           >  2:322785/1‑149 (MQ=255)
                                                                                                                      tATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATtaata                         >  2:43644/1‑149 (MQ=255)
                                                                                                                      tATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATtaata                         <  1:1450000/149‑1 (MQ=255)
                                                                                                                      tATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATtaata                         <  1:1449965/149‑1 (MQ=255)
                                                                                                                      tATCTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATtaata                         <  1:1012413/149‑1 (MQ=255)
                                                                                                                         cTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATTAataata                      <  1:989083/149‑1 (MQ=255)
                                                                                                                         cTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATTAataata                      >  1:1548249/1‑149 (MQ=255)
                                                                                                                         cTAACGTAGATAATGTTTGGTTTTCATTGTTTTTAGAAACTTTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATTAataata                      >  1:1264572/1‑149 (MQ=255)
                                                                                                                             cGTAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATTAATAATAtttt                  <  1:13360/149‑1 (MQ=255)
                                                                                                                               tAGTTAATGTTTTGTTTTCCTTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATTAATAATATTTTca                <  2:722829/149‑1 (MQ=255)
                                                                                                                               tAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGGTTGGTTCGCATTTGTACCGTTGGCTTTACTAAtattatatccc                >  2:423624/1‑144 (MQ=255)
                                                                                                                               tAGATAATGTTTGGTTTTCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATTAATAATATTTTca                >  1:547612/1‑149 (MQ=255)
                                                                                                                                  aTAATGTTTGGTTTTCATTGTTTTTTTCTTTTTTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCTACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATTAATAATATTTTcagca             >  2:1603435/1‑149 (MQ=255)
                                                                                                                                          ttggttttCATCGTTTGAAGACTCGGTGACTTTTCTATCACCATTCAAAGCTTGTTTCGTAGTTGTTCCAGTTGAACGTGCTGCTCCAATTTGTAATTGGTTCGCATTTGTACCATTTGCTTGTTTAATAATATTTTCACCAGAAGttt      >  2:542836/1‑148 (MQ=255)
                                                                                                                                           tggtttcATTTTTTTTGGCATCTGTTACTTTTCTACCACCCTTCATCGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGTTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATTAATAATATTTTCAGCAGCAGTTAcc    <  2:146900/149‑1 (MQ=255)
                                                                                                                                            tgttttCTTTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATTAATAATATTTTCAGCAGCAGTTAcc    <  1:565847/148‑1 (MQ=255)
                                                                                                                                             gttttCATTGTTTTTAGCATCTGTTACTTTTCTATCCCCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATTAATAATATTTTCAGCAGCAGTTACCg   <  2:1367134/149‑1 (MQ=255)
                                                                                                                                             gttttCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATTAATAATATTTTCAGCAGCAGTTACCg   <  2:1159914/149‑1 (MQ=255)
                                                                                                                                              ttttCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATTAATAATATTTTCAGCAGCAGTTACCGc  >  1:1616683/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
AAGTTACCCATCGCTGTATTCAGTTCCTGAGCGGTTTGAATGGCTTGCGTTACTTCAGCTACAGTGTGCGCTTGATTGATGTTTCCAGTAACAGCACCTTTTTGTGCATTGTTTAAGTTATCTAACGTAGATAATGTTTGGTTTGCATTGTTTTTAGCATCTGTTACTTTTCTATCACCATTCAACGCTTGTTTCGTAGTTGTTACAGTTGAAAGTGCTGCTTCAACTTGTGATTGGTTCGCATTTGTACCATTTGCTTGATTAATAATATTTTCAGCAGCAGTTACCGC  >  USA300TCH1516_ALE/1481211‑1481500

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: