BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	1,513,930	A→T	L413F (TTA→TTT)	USA300HOU_RS07375 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,513,930	0	A	T	100.0%	331.4 / NA	103	L413F (TTA→TTT)	USA300HOU_RS07375	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (41/62); total (41/62)

Only 100 of 103 total aligned reads displayed.

AAAATCATTAATTTATTTGTATGTATCGCTTTATTAGTAGCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTAATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAATAATGCACTTAAAGTTTTGAACTGACGAAATTTACAAAATGATTC  >  USA300TCH1516_ALE/1513784‑1514074

aaaaTCATTAATTTATTTGAATGTATCGCTTTATTAGTAGCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTAt                                                                                                                                                <  2:242876/149‑1 (MQ=255)

aaaaTCATTAATTTATCTGGATGTATCGCTTAATTAGGAGCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTAt                                                                                                                                                <  2:479885/149‑1 (MQ=255)

 aaaTCATTAATTTATTTGTATGTATCGCTTTATTAGTAGCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTAtt                                                                                                                                               >  2:1687093/1‑149 (MQ=255)

 aaaTCATTAATTTATTTGTATGTATCGCTTTATTAGTAGCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATATTTTAtt                                                                                                                                               >  2:177406/1‑149 (MQ=255)

  attCATTTAATTATTGGTCTGTATCCCTGTACTAGTAGCCATTGGCATGGGGTTTGTCTCTGGGATAAAGCGTACAAGAAATACTGCAATCAAAGTACCACATCGCGTATAAAATCCCGCATCTATATTACGCATAATATGTTTTATTg                                                                                                                                              <  2:768138/147‑1 (MQ=255)

   attatgaaTTTATTTGTATGTATCGCTTTATTAGTAGCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGt                                                                                                                                             <  2:850076/143‑1 (MQ=255)

   aTCCTTAATTTATTTGTCTGTATCGCTTTATTAGTAGCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATGCTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGt                                                                                                                                             <  2:1151964/149‑1 (MQ=255)

   aTCATTAATTTATTTGTATGTTTCGCTTTATTAGTAGCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGt                                                                                                                                             <  2:892315/149‑1 (MQ=255)

   aTCATTAATTTATTTGTATGTATCGCTTTATTAGTAGCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGt                                                                                                                                             <  1:56461/149‑1 (MQ=255)

   aTCATTAATTTATTTGTATGTATCGCTTTATTAGTAGCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGt                                                                                                                                             <  2:513836/149‑1 (MQ=255)

   aTCAATAATTTATTTGTATGTATCGCTTTATTAGTAGCCATTGGCATGGGGTGTGTCTCTTGGAAAAAAAAAAAAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGt                                                                                                                                             <  2:907699/149‑1 (MQ=255)

    tCATTAATTTATTTGTATGTATTGCTTTATTAGTAGCCATTGGAAGGGGGTTTGTCTCTTGGATAAAGCGCACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAACACCAGCATCTATATCACTCATAATATGTTTTATTGTa                                                                                                                                            <  2:667080/149‑1 (MQ=255)

      aTTAATTTATTTGTATGTATCGCTTTATTAGTAGCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTAtt                                                                                                                                          <  1:1308612/149‑1 (MQ=255)

       ttACTTTATTTGTATGTATCGCTTTATTAGTAGCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTa                                                                                                                                         <  1:75417/149‑1 (MQ=255)

       ttAATTTATTTTTATGTATCGCTTTATTAGTAGCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTa                                                                                                                                         <  1:864316/149‑1 (MQ=255)

       ttAATTTATTTGTATGTATCGCTTTATTAGTAGCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTa                                                                                                                                         <  1:542106/149‑1 (MQ=255)

       ttAATTTATGTGTTTGTTTCATTGGAGTAGTAGCCATTGGCATGGGGTTTGTCTCTTGAATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTa                                                                                                                                         <  2:509848/149‑1 (MQ=255)

        tAATTTATTTGTATGTATCGCTTTATTAGTAGCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAAGAAAAGTGCCACATCTCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAg                                                                                                                                        >  2:1288730/1‑149 (MQ=255)

        tAATTTATTTGTATGTATCACTTTGTTAGTAGCCATTGGCATGGGGTTTGTGTCTTGGATAAAGCGTACAGAAAATACTGCAATAAAAGTACCACATCGCGTAGAAAAACCAGAATCTATATCACTCATAATATGTTGTATTGTATTAg                                                                                                                                        >  2:834931/1‑149 (MQ=255)

           tttatttGTATGTATCGCTTTATTAGTAGCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATTGCGTAAAAAAACCAGCAGCTATATCACTCATAATATGTTTTATTGTATTAGGat                                                                                                                                     <  1:1529768/149‑1 (MQ=255)

           tttatttGTATGTACCGCTTTATTAGTAGCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCGTAATATGTTTTATTGTATTAGGat                                                                                                                                     <  1:854846/149‑1 (MQ=255)

                 tgtatgtaTCGCTTTATTAGTAGCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTaa                                                                                                                               >  1:1501230/1‑149 (MQ=255)

                   tatgtacCGCTTTATTAGGAGCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATg                                                                                                                             <  2:1542919/149‑1 (MQ=255)

                      gtatCGCTTTATTAGTAGCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCa                                                                                                                          >  1:1409732/1‑149 (MQ=255)

                            cTTTATTAGTAGCCAGTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTAttt                                                                                                                    <  2:131288/149‑1 (MQ=255)

                            cTTTATTAGGAGCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTAttt                                                                                                                    <  2:141522/149‑1 (MQ=255)

                             tttATTAGTAGCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTg                                                                                                                   >  1:673044/1‑149 (MQ=255)

                              ttattaGTAGCCATTGTAATTGGGTTTGTCTCTTGGACAAAGCGCACAAATAATACTGCAATAAAAGATCCACATCGCGTAAAAAATCCAGCATCTATCTCACTCATAATATGTTTTATTGTAGTAGGATTATTAATGCCATTATTTgg                                                                                                                  <  2:1134045/149‑1 (MQ=255)

                              ttattaGTAGCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTgg                                                                                                                  <  1:1433128/149‑1 (MQ=255)

                                   agtagCCCTTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAATACCAGCATCTATTTCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTAt                                                                                                             <  1:1520971/149‑1 (MQ=255)

                                    gtagCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATCTCATTAATATGAAGAAATATTGTATTAAGATTATTAATGCAATTATTTGGATTATc                                                                                                            >  1:1681115/1‑149 (MQ=255)

                                    gtagCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTAta                                                                                                            >  1:1394795/1‑148 (MQ=255)

                                    gtagCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATc                                                                                                            >  1:1412807/1‑149 (MQ=255)

                                     tagCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATTTCCCTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCa                                                                                                           <  1:909474/149‑1 (MQ=255)

                                      ggCCATTGTCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCAc                                                                                                          <  2:1540138/148‑1 (MQ=255)

                                        ccATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATCCTGCAATAAAAGTACCACATCGCGTAAAAAAACAAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACtt                                                                                                        >  2:119585/1‑149 (MQ=255)

                                         cATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGCTTATTAATGCCATTATTTGGATTATCACTTa                                                                                                       >  1:547296/1‑149 (MQ=255)

                                         cATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTa                                                                                                       >  1:1549009/1‑149 (MQ=255)

                                          aTTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGACATTATTTGGATTATCACtttt                                                                                                      >  2:1170079/1‑147 (MQ=255)

                                           ttGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTTTc                                                                                                     >  1:145065/1‑149 (MQ=255)

                                                aTGGGGTTTGTCTATTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTGAAAAAACAAGCATCTATATCACTCATAATATGATTTATTATATTAGAATTATTAATGCCATTATTTGGATTGTTACTTATCCTTGt                                                                                                >  2:1609970/1‑149 (MQ=255)

                                                     gTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTAATAGGAATATTAATGCCATTTTTTGGATTATCACTTATCCTTGTATTta                                                                                           >  2:90748/1‑149 (MQ=255)

                                                         gTCTATTGGATAAAGCGTACAAAAAATACTGAAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAAt                                                                                       <  2:198917/149‑1 (MQ=255)

                                                           ctctTGGATAAAGCGTAAAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTAGTTGGATTATCACTTATCCTTGTATTTATAATTg                                                                                     <  1:583072/149‑1 (MQ=255)

                                                             ctTGGATAAAGCGTATAAAGAATACTGCAAATAAAGTACTAATTCGCGTAAAAAAACCAGGATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGaa                                                                                   <  2:218618/149‑1 (MQ=255)

                                                             ctTGGATAAAGCATACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGaa                                                                                   <  2:905037/149‑1 (MQ=255)

                                                             cgtGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGaa                                                                                   <  2:1355277/147‑1 (MQ=255)

                                                             cagagATAAAGCGCACAAAACATACTGCAATAAAAGTACCACAACAAAAAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGaa                                                                                   <  2:485976/145‑1 (MQ=255)

                                                               tGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAAtt                                                                                 >  2:931407/1‑149 (MQ=255)

                                                                gaaaaaaaCGTAAAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATCATTAATGCCATTATTTGGATTATCACTTATCCTTGTCTTTATAATTGAATTa                                                                                <  2:1215317/145‑1 (MQ=255)

                                                                       gCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAAtattat                                                                         >  1:1656032/1‑149 (MQ=255)

                                                                       gCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAAtattat                                                                         >  1:441516/1‑149 (MQ=255)

                                                                        cGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATtata                                                                        >  2:217076/1‑149 (MQ=255)

                                                                                  aTACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGAt                                                              <  1:637933/149‑1 (MQ=255)

                                                                                      tGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGAtcgtc                                                          <  1:1403816/149‑1 (MQ=255)

                                                                                      tGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGAtcgtc                                                          <  1:500910/149‑1 (MQ=255)

                                                                                      tGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACCCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGAtcgtc                                                          >  2:861913/1‑149 (MQ=255)

                                                                                         aaTAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTg                                                       <  1:637574/149‑1 (MQ=255)

                                                                                         aaTAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTg                                                       <  2:1500223/149‑1 (MQ=255)

                                                                                          aTAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGc                                                      <  1:1589584/149‑1 (MQ=255)

                                                                                          aTAAAAGGACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGc                                                      <  1:941909/149‑1 (MQ=255)

                                                                                              aaGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTaaa                                                  <  2:712816/149‑1 (MQ=255)

                                                                                              aaGTACCACATAAAAAAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTaaa                                                  <  2:1275012/149‑1 (MQ=255)

                                                                                                 tACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACaa                                               <  2:1682690/149‑1 (MQ=255)

                                                                                                 tACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAAGGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACaa                                               <  2:186235/149‑1 (MQ=255)

                                                                                                  aCCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACaat                                              >  2:1330081/1‑149 (MQ=255)

                                                                                                      caTCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAATAATg                                          >  1:830831/1‑149 (MQ=255)

                                                                                                       aTCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAATAATGc                                         >  1:1646267/1‑149 (MQ=255)

                                                                                                        tCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATCGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAATAATGCa                                        <  1:1069884/149‑1 (MQ=255)

                                                                                                         cgcgTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAATAATGCAc                                       <  1:1409536/149‑1 (MQ=255)

                                                                                                         cgcgTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAATAATGCAc                                       <  2:130457/149‑1 (MQ=255)

                                                                                                          gcgTAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAATAATGCACt                                      <  2:1076249/149‑1 (MQ=255)

                                                                                                             tAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAATAATGCACTTaa                                   >  2:440924/1‑149 (MQ=255)

                                                                                                             tAAAAAAACCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAATAATGCACTTaa                                   >  1:1565632/1‑149 (MQ=255)

                                                                                                                aaaaaCCAGCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAATAATGCACTTAAAGt                                <  1:50905/149‑1 (MQ=255)

                                                                                                                aaaaaCCAGCATCTATATAACTGCTAATATGTTATATTGTAATAGGATTATTAATGCCATTATTTGGATGATCGCTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAATAATGCACTTAAAGt                                >  2:834458/1‑149 (MQ=255)

                                                                                                                   aaCCAGCATCTATATCAATCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCCAAACAATAATGCACTTAAAGtttt                             <  1:857739/149‑1 (MQ=255)

                                                                                                                        tcATGTATATCACTCCTAATATTTATTAATGTAGTAGGATTATTAATGCCATTATTTCGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAATATCGTCTTGCTAAACAATAATGCACTTAAAGTTTTGAACt                        <  1:643635/148‑1 (MQ=255)

                                                                                                                        gCATCTATATCCCTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAATAATGCACTTAAAGTTTTGAACt                        <  1:1467792/149‑1 (MQ=255)

                                                                                                                        gCATCTATATCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAATAATGCACTTAAAGTTTTGAACt                        <  1:457867/149‑1 (MQ=255)

                                                                                                                         catttttatCAATCATAATATGTTTTATTGTATTAGTATTATTAATGCCATTATTTGAATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAATAATGCACTTAAAGTTTTGAACTg                       <  2:152975/143‑1 (MQ=255)

                                                                                                                                 tCACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAATAATGCACTTAAAGTTTTGAACTGACGAAAtt               <  1:650570/149‑1 (MQ=255)

                                                                                                                                  cgcTCATAATTTGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAATAATGCACTTAAAGTTTTGAACTGACGAAAttt              <  2:66735/147‑1 (MQ=255)

                                                                                                                                  cACTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAATAATGCACTTAAAGTTTTGAACTGACGAAAttt              <  1:904627/149‑1 (MQ=255)

                                                                                                                                  cACTCATAATATCTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAATAATGCACTTAAAGTTTTGAACTGACGAAAttt              >  1:1170269/1‑149 (MQ=255)

                                                                                                                                   aCTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTTGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAATAATGCACTTGAAGTTTTGAACTGACGAAGTTTa             >  2:41663/1‑149 (MQ=255)

                                                                                                                                   aCTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAATAATGCACTTAAAGTTTTGAACTGACGAAATTTa             <  1:1668824/149‑1 (MQ=255)

                                                                                                                                   aCTCATAATATGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAATAATGCACTTAAAGTTTTGAACTGACGAAATTTa             >  1:539552/1‑149 (MQ=255)

                                                                                                                                   aCTCATAATATGTTTTATTGTATTAGGATGATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAATAATGCACTTAAAGTTTTGAACTGACGAAATTTa             <  2:671875/149‑1 (MQ=255)

                                                                                                                                     tCATAAGATGTTTTATTGTAGTAGGATTATTAATGCCATTATTTGGATTATCCCTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAATAATTCAATTAAAGTTTTGAACTGACGCAATTTACa           >  2:877142/1‑149 (MQ=255)

                                                                                                                                       ataataTGTTTTATTGTATTAGGATTATTAATGCCTTTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAATAATGCACTTAAAGTTTTGAACTGACGAAATTGACaaa         >  2:371922/1‑149 (MQ=255)

                                                                                                                                       ataataTGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAATAATGCACTTAAAGTTTTGAGCTGACGAAATTTAGaaa         >  2:1154318/1‑149 (MQ=255)

                                                                                                                                       ataataTGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAATAATGCACTTAAAGTTTTGAACTGACGAAATTTACaaa         <  1:51621/149‑1 (MQ=255)

                                                                                                                                         aataTGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAATAATGCACTTAAAGTTTTGAACTGACGAAATGTaaaacaa       >  2:1715274/1‑146 (MQ=255)

                                                                                                                                            atGTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAATAATGCACTTAAAGTTTTGAACTGACGAAATTTACAAAATGAt    >  1:194162/1‑149 (MQ=255)

                                                                                                                                              gTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAATAATGCACTTAAAGTTTTGAACTGACGAACTTTACAAAATGACtc  >  2:112181/1‑149 (MQ=255)

                                                                                                                                              gTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAATAATGCACTTAAAGTTTTGAACTGACGAAATTTACAAAATGATtc  >  1:1455463/1‑149 (MQ=255)

                                                                                                                                              gTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAATAATGCACTTAAAGTTTTGAACTGACGAAATTTACAAAATGATtc  >  1:1591301/1‑149 (MQ=255)

                                                                                                                                              gTTTTATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAATAATGCACTTAAAGTTTTGAACTGACGAAATTTACAAAATGATtc  >  2:28255/1‑149 (MQ=255)

                                                                                                                                              gTTTTATTGTATTAGGATTATTAATGCCACTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACCATAAAACACTTAAAGTTTTGAACTGACGAAATTTACAAAATGATtc  >  1:896855/1‑149 (MQ=255)

AAAATCATTAATTTATTTGTATGTATCGCTTTATTAGTAGCCATTGGCATGGGGTTTGTCTCTTGGATAAAGCGTACAAAAAATACTGCAATAAAAGTACCACATCGCGTAAAAAAACCAGCATCTATATCACTCATAATATGTTTAATTGTATTAGGATTATTAATGCCATTATTTGGATTATCACTTATCCTTGTATTTATAATTGAATTAATATTATATATTAAAGATCGTCGTGCTAAACAATAATGCACTTAAAGTTTTGAACTGACGAAATTTACAAAATGATTC  >  USA300TCH1516_ALE/1513784‑1514074

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`