BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	1,646,189	G→C	A155G (GCA→GGA)	ypdF ←	Aminopeptidase YpdF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,646,189	0	G	C	98.5%	433.8 / NA	129	A155G (GCA→GGA)	ypdF	Aminopeptidase YpdF
Reads supporting (aligned to +/- strand): ref base G (0/0); major base C (60/67); minor base T (0/2); total (60/69)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 130 total aligned reads displayed.

TTTCAATAATTTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTGCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATACGGCTTTT  >  USA300TCH1516_ALE/1646043‑1646331

tttCAATAATTTTCTCACTTGCAACACCATGTGGTAATGCCCCTCTATGACCAGATGCCACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATGCTTTTTCAGTCATGcctcct                                                                                                                                              <  1:518388/149‑1 (MQ=255)

 ttCAATACTTTTCTCACTTGCAACACCATGTGGTAAGGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCtt                                                                                                                                             <  2:512657/149‑1 (MQ=255)

 ttCAATAATTTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTGATTCTTTTTCAGTCATGCCTCCtt                                                                                                                                             <  1:857484/149‑1 (MQ=255)

 ttCAATAATTTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCtt                                                                                                                                             <  1:548977/149‑1 (MQ=255)

 ttCAATAATTTTATCACCTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCtt                                                                                                                                             <  2:1358229/149‑1 (MQ=255)

  tCAATAATTTTATCACTTGCAACACCATGTTGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCttt                                                                                                                                            <  2:885379/149‑1 (MQ=255)

  tCAATAATTTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCttt                                                                                                                                            <  2:1555472/149‑1 (MQ=255)

   cAATAATTTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCtttt                                                                                                                                           <  1:64775/149‑1 (MQ=255)

    aataatTTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTa                                                                                                                                          <  1:1062096/149‑1 (MQ=255)

    aataatTTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTa                                                                                                                                          <  1:87427/149‑1 (MQ=255)

    aataatTTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTa                                                                                                                                          <  2:1076402/149‑1 (MQ=255)

    aataatTTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTa                                                                                                                                          <  1:167486/149‑1 (MQ=255)

    aataatTTTATCACTCGCAACCCCATGTGGTAATCCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTCCTCCTCATTCTAGCATTTTGCTTTCTAATATTGCCTTCAATTCTTTTTCAGTCATGCCTCCTTTTa                                                                                                                                          <  1:1047415/149‑1 (MQ=255)

    aacaatTTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTa                                                                                                                                          <  1:1482051/146‑1 (MQ=255)

     ataatTTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTac                                                                                                                                         >  2:899721/1‑149 (MQ=255)

       aatTTTATCACTTGCAACCCCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCCAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTacaa                                                                                                                                       <  2:239330/149‑1 (MQ=255)

        atTTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTTCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTtcaac                                                                                                                                      >  2:1463530/1‑149 (MQ=255)

         ttttATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTccaaca                                                                                                                                     >  2:482181/1‑149 (MQ=255)

          tttATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAg                                                                                                                                    <  2:194263/149‑1 (MQ=255)

           tgaTCACTTGCACCACTATGTGGTAATGCTCCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATGCGAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGt                                                                                                                                   <  2:713091/147‑1 (MQ=255)

            tATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCGAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGtt                                                                                                                                  <  2:139225/149‑1 (MQ=255)

              ttaCTTGCAACTCCATGTGGTAATGCACCTCTATGACCCGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTaa                                                                                                                                <  2:765585/147‑1 (MQ=255)

                aCTTGCAACACCATGTGGTAATGCACCTCTATGATCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCTTAATTCTAGCATTTTGCTTTATTATATTGCCTTTAATTTTTTTTCAGTCATGCCTCCTGTTACAACAGTTaaaa                                                                                                                              <  2:1006235/149‑1 (MQ=255)

                 cTTGCAACACCATAGGGTAATGCACCTCTGTGTCCATGTACGACAATCGTATCGAATGATGGTCCATCTGCTCCAAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAat                                                                                                                             >  1:1266913/1‑149 (MQ=255)

                   tGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAatat                                                                                                                           >  2:522173/1‑149 (MQ=255)

                     cAACTCCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTACTATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAatatat                                                                                                                         <  2:1112654/149‑1 (MQ=255)

                     cAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTCTTTAGTCTAATCTTGCCTCTCTGGGTGTTTCAGTCATGCCTCCTTTTACAACAGTTAAAatatat                                                                                                                         <  1:1695934/149‑1 (MQ=255)

                      aaCACCATGTGGTAATGCACCTCTATGACCAGCTGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCCGTCATCCCTCCTTTTGANACAGTTAAAATATAtt                                                                                                                        >  1:1588606/1‑149 (MQ=255)

                      aaCACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTTCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACCGTTAAAATATAtt                                                                                                                        >  2:913509/1‑149 (MQ=255)

                      aaCACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATAtt                                                                                                                        >  1:970985/1‑149 (MQ=255)

                      aaCACCAGGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCAAATGATGGTCCATCCGCTCCCAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTCCTTCAGTCATGCCTCCTTTTTCACCAGTTAACATATAtt                                                                                                                        >  2:852002/1‑149 (MQ=255)

                       acacCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATAttt                                                                                                                       >  1:1066563/1‑148 (MQ=255)

                          ccATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCAGTCGTCCTTTTACAACAGTTAAAATATATTtata                                                                                                                    >  1:126360/1‑149 (MQ=255)

                            aTGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATg                                                                                                                  <  1:588571/149‑1 (MQ=255)

                                 gTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTtca                                                                                                             >  1:1714890/1‑149 (MQ=255)

                                 gTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGACTCCTTTTACAACAGTTACAATATATTCATATGTCtca                                                                                                             >  2:974912/1‑149 (MQ=255)

                                    aTGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCACGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTtcatca                                                                                                          <  1:1526417/149‑1 (MQ=255)

                                     tGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATcaa                                                                                                         <  2:561823/149‑1 (MQ=255)

                                               tGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACCATATTAgc                                                                                               >  2:1324433/1‑149 (MQ=255)

                                                gACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAgct                                                                                              >  2:935262/1‑149 (MQ=255)

                                                gACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATAGTAtct                                                                                              >  2:1635862/1‑149 (MQ=255)

                                                 aCCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAgctg                                                                                             >  1:1575627/1‑149 (MQ=255)

                                                     gATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCCTGCCCCAATATACTACAGTTAAAATATATTCACATGTTTCATCAACAATATTAGCTGCttt                                                                                         <  1:1343399/149‑1 (MQ=255)

                                                      aTGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCtttt                                                                                        >  1:1120030/1‑149 (MQ=255)

                                                      aTGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTATCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCtttt                                                                                        >  2:1102826/1‑149 (MQ=255)

                                                      aTGCTACAATCGTATCGAATGATAGTCGATCTGCTCCTAATTCTAGCATTATGCTTACTAATATTGCCTTTAGTTGTTTTACTGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCtttt                                                                                        >  1:1284725/1‑149 (MQ=255)

                                                        ggtACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTg                                                                                      <  1:1189394/147‑1 (MQ=255)

                                                           aCAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCCACAATATTAGCTGCTTTTTGAAt                                                                                   >  2:839068/1‑149 (MQ=255)

                                                           aCAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAAt                                                                                   >  1:178410/1‑149 (MQ=255)

                                                                  tATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCa                                                                            >  1:290118/1‑149 (MQ=255)

                                                                    tCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACACTAAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAAt                                                                          >  1:820294/1‑149 (MQ=255)

                                                                     cGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAAtt                                                                         >  1:1338289/1‑149 (MQ=255)

                                                                       aaTGATGGTCCATCTGCGCCTGATTCTAGCATTTTGCTTTCTAATATTGCCTTGAATTCTGGTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAAGATTAGCTGCTTTTTGAATTAAAGCAATTTc                                                                       <  1:352077/149‑1 (MQ=255)

                                                                               tCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCAt                                                               >  2:1472768/1‑149 (MQ=255)

                                                                                 cATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCt                                                             <  2:1502779/149‑1 (MQ=255)

                                                                                   tcttcttctAATTCTAGCATTTTGCTTTCTAATCTTGCCTTTAATTCTTTTTTAGTCATGCCTTCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCttt                                                           <  2:1377124/149‑1 (MQ=255)

                                                                                     tGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGa                                                         >  2:1264911/1‑149 (MQ=255)

                                                                                     tGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCATCAGCATCTTTGa                                                         >  2:56262/1‑149 (MQ=255)

                                                                                       cTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCGCTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACg                                                       <  2:1575627/149‑1 (MQ=255)

                                                                                          cTAATTCTGGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACTACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGtct                                                    <  2:1038858/149‑1 (MQ=255)

                                                                                          cTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGtct                                                    <  2:1484092/149‑1 (MQ=255)

                                                                                          cTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGtct                                                    <  2:1709461/149‑1 (MQ=255)

                                                                                          cTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCCTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGtct                                                    <  2:1147316/149‑1 (MQ=255)

                                                                                           tAATTCTAGCATTTTGCTTTCTAACATTGCCTTTAGTTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGtctc                                                   >  1:855359/1‑149 (MQ=255)

                                                                                              ttCTAGCATTTTGCTCTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTaa                                                <  2:1448379/149‑1 (MQ=255)

                                                                                               tCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAAt                                               >  1:1583299/1‑149 (MQ=255)

                                                                                                   gCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAGTATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTACTTTTa                                           >  2:433845/1‑149 (MQ=255)

                                                                                                    cATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTAt                                          <  1:105377/149‑1 (MQ=255)

                                                                                                    cATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTAt                                          >  2:1205504/1‑149 (MQ=255)

                                                                                                         tgttttCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGCTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACa                                     <  1:966522/146‑1 (MQ=255)

                                                                                                         tGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACa                                     <  1:719142/149‑1 (MQ=255)

                                                                                                         tGCGTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACa                                     <  2:1633693/149‑1 (MQ=255)

                                                                                                                   tatTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGaaa                           >  2:887177/1‑149 (MQ=255)

                                                                                                                   tatTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGaaa                           >  1:546678/1‑149 (MQ=255)

                                                                                                                   tatTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTACTCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGaaa                           >  1:1334977/1‑149 (MQ=255)

                                                                                                                    atTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACACTATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGGAAt                          >  1:676435/1‑149 (MQ=255)

                                                                                                                    atTGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAAt                          >  1:258708/1‑149 (MQ=255)

                                                                                                                     ttGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAAtt                         >  2:459790/1‑148 (MQ=255)

                                                                                                                     ttGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATg                         <  1:79769/149‑1 (MQ=255)

                                                                                                                     ttGCCTTTAATTCTTTGTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCGTATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTGTTGGAAATg                         >  2:1541799/1‑149 (MQ=255)

                                                                                                                      tGCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGc                        >  2:571215/1‑149 (MQ=255)

                                                                                                                       gCCTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGTCGTCTCTAATTTTATCTACAGTATTAGAAATGCt                       >  1:177424/1‑149 (MQ=255)

                                                                                                                         cTTTAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCCTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCtta                     <  1:1366411/149‑1 (MQ=255)

                                                                                                                         cTTTAATGCTTTTTCACTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCTTCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCtta                     <  1:891634/149‑1 (MQ=255)

                                                                                                                          tttAATTCTTTTTCAGTCTTGCCTCCTTTTACAACAGTTAATATATATTCATATGTTTCATCTACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGTCGTCTCTAATTTTATCTATAGTATTAGAAATGCttat                    <  2:1689436/149‑1 (MQ=255)

                                                                                                                          tttAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCttat                    <  2:1444491/149‑1 (MQ=255)

                                                                                                                          tttAATTCTTTTTCAGTCATGCCTCCTGTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAATGCAATTTCGTCAGCATCTTTGACGTTTTTCGGGTTATCTACAGTATTAGAAATGCttat                    <  2:1223025/149‑1 (MQ=255)

                                                                                                                            tAATTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCttatta                  <  1:992776/149‑1 (MQ=255)

                                                                                                                              aTTCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATTTTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAGAGCAATTTCGTCAGCATCTTTTACGTCTCTACTTTTATCTAATGTATTAGAAATGCTTATTAAt                >  2:1381437/1‑149 (MQ=255)

                                                                                                                               ttCTTTTTCAGTCATGCCTTCTTTTACAACAGTTAACATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATg               <  1:1536031/149‑1 (MQ=255)

                                                                                                                               ttCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTGGAAATGCTTATTAATg               <  2:470566/149‑1 (MQ=255)

                                                                                                                               ttCTTTTTCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATg               <  2:1518695/149‑1 (MQ=255)

                                                                                                                                   ttttCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGatat           >  1:1554362/1‑149 (MQ=255)

                                                                                                                                   ttttCAGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGatat           >  1:1192938/1‑149 (MQ=255)

                                                                                                                                        aGTCATGCCTCCTTTTACAACCGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTACTGatactcttc      <  2:17734/149‑7 (MQ=255)

                                                                                                                                        aGTCATGCCTCCTTTTACAACAGTTAAAATATATTTATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTCCAGTATTAGAAATGCTTATTAATGATATACggt      >  2:1574444/1‑148 (MQ=255)

                                                                                                                                        aGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATACGGc      <  1:770942/149‑1 (MQ=255)

                                                                                                                                        aGTCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATATTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATACGGc      >  2:1351072/1‑149 (MQ=255)

                                                                                                                                        aGTCATGCCTCCTTTTACAACAGTTAAAATATATACATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATACGGc      >  2:1034009/1‑149 (MQ=255)

                                                                                                                                          tCATGCCTCCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATACGGCtt    >  1:1652924/1‑149 (MQ=255)

TTTCAATAATTTTATCACTTGCAACACCATGTGGTAATGCACCTCTATGACCAGATGCTACAATCGTATCGAATGATGGTCCATCTGCTCCTAATTCTAGCATTTTGCTTTCTAATATTGCCTTTAATTCTTTTTCAGTCATGCCTGCTTTTACAACAGTTAAAATATATTCATATGTTTCATCAACAATATTAGCTGCTTTTTGAATTAAAGCAATTTCGTCAGCATCTTTGACGTCTCTAATTTTATCTACAGTATTAGAAATGCTTATTAATGATATACGGCTTTT  >  USA300TCH1516_ALE/1646043‑1646331

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`