BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	2,143,110	G→A	V163V (GTG→GTA)	USA300HOU_RS10935 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,143,110	0	G	A	99.3%	458.6 / NA	144	V163V (GTG→GTA)	USA300HOU_RS10935	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (71/72); total (72/72)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 144 total aligned reads displayed.

TACAGAAAAGCTAATTATTATTGCTCGAAAAATACCTATATTTATCTTCTTTGTATCTATTATTGGTCCTTTATTAGAAGAATATGTATTCAGAAAAGTAATCTTTGGAGAATTATTTAATGCGATTAAAGGTAATCGTATCGTGGCATTTATTATTGCTACAACAGTAAGTTCATTAATATTTGCATTAGCACATAATGATTTCAAATTTATTCCAGTTTATTTTGGTATGGGTGTCATTTTTTCATTAGCATATGTTTGGACAAAACGGCTTGCTGTTCCAATT  >  USA300TCH1516_ALE/2142966‑2143251

tACAGAAAAGCTAATTCTTATTGCTCGAAAAATACCTATATATCACCACCTTTCAAAAACTATTTTTTCTTTATTAGAAGAATATGTATTCAGAAAAGTAATCTTTGGAGAATTATTTAATGCGATTAAAGGTAATCGTATCGTAGCAt                                                                                                                                           >  1:1260136/1‑149 (MQ=255)

tACAGAAAAGCTAATTATTATTGCTCGAAAAATACCTATATTTATCTTCTTTGTATCTATTATTGGTCCTTTATTAGAAGAATATGTATTCAGAAAAGTAATCTTTGGAGAATTATTTAATGCGATTAAAGGTAATCGTATCGTAGCAt                                                                                                                                           <  2:601683/149‑1 (MQ=255)

tACAGAAAAGCTAATTATTATTGCTCGAAAAATACCTATATTTATCTTCTTTGTATCTATTATTGGTCCTTTATTAGAAGAATATGTATTCAGAAAAGTAATCTTTGGAGAAATATTTAATGCGATCAAAGGTAATCGTATGGTAGCag                                                                                                                                           <  2:1008093/149‑2 (MQ=255)

 aCAGAAAAGCTAGTTATTATTGCTCGAAAAATACCTATATTTATCTTCTTTGTATCTATTATTGGTCCTTTATTAGAAGAATATGTATTCAGATCATGATTCTTTGGAGAATTATTTAATGCGATTAAAGGTAATCGTATCGTAGCAtt                                                                                                                                          >  1:1172784/1‑149 (MQ=255)

 aCAGAAAAGCTAATTATTATTGCTCGAAAAATACCTATATTTATCTTCTTTGTATCTATTATTGGTCCTTTATTAGAAGAATATGTATTCAGAAAAGTAATCTTTGGAGGATTATTTAATGCGATTAAAGGTAATCGTATCGTAGCAtt                                                                                                                                          >  2:1020837/1‑149 (MQ=255)

  cAGAAAAGCTAATTATTATTGCTCGAAAAATACCTATATTTATCTTCTTTGTATCTATTATTGGTCCTTTATTAGAAGAATATGTATTCATAAAAGTAATCTTTGGAGAATTATTTTATGCGATTAAAGGTAATCGTATCGTAGCAttt                                                                                                                                         <  1:1257370/149‑1 (MQ=255)

  cAGAAAAGCTAATTATTATTGCTCGAAAAATAACTAGATTTATCTTCTTTGTATCTATTATTGGTCCTTTATTAGAAGAATATGTATTCAGAAAAGTAATCTTTGGAGAATTATTTAATGCGCTTAAAGGTAATCGTATCGTGGCAttt                                                                                                                                         >  1:1093931/1‑149 (MQ=255)

   aGAAAAGCTAATTATTATTGCTCGAAAATTACCTTTATTTATCTTCTTTGTATCTATTATTGGTCCTTTATTAGAAGAATATGTATTCAGAAAAGTAATCTTTGGAGAATTATTTAATGCGATTAAAGGTAATCGTATCGTAGCATtta                                                                                                                                        <  2:876495/149‑1 (MQ=255)

   aGAAAAGCTAATTATTATTGCTCGAAAAATACCTATATTTATCTTCTTTGTATCTATTATTGGTCCTTTATTAGAAGAATATGTATTCAGAAAAGTAATCTTTGGAGAATTATTTAATGCGATTAAAGGTAATCGTATCGTAGCATtta                                                                                                                                        <  2:1529114/149‑1 (MQ=255)

   aGAAAAGCTAATTATTATTGCTCGAAAAATACCTATATTTATCTTCTTTGTATCTATTATTGGTCCTTTATTAGAAGAATATGTATTCAGAAAAGTAATCTTTGGAGAATTATTTAATGCGATTAAAGGTAAGCGTATCGTAGCATtta                                                                                                                                        >  1:424657/1‑149 (MQ=255)

   aGAAAAGCTAATTATTATTGCTCGAAAAATACCTATATTTATCTTCTTTGTATCTATTATTGGTCCTTTATTAGAAGAATATGTATTCAGAAAAGTAATCTTTGGAGAATTATTTAATGCGATAAAAGGTAATCGTATCGTAGCATtta                                                                                                                                        >  1:1535958/1‑149 (MQ=255)

     aaaaGCTAATTATTATTGCTCGAAAAATACCTATATTTATCTTCTTTGTATCTATTATTGGTCCTTTATTAGAAGAATATGTATTCAGAAAAGTAATCTTTGGAGAATTATTTAATGCGATTAAAGGTAATCGTATCTTAGCATttatt                                                                                                                                      >  2:191268/1‑149 (MQ=255)

     aaaaGCTAATTATTATTGCTCGAAAAATACCTATATTTATCTTCTTTGTATCTATTATTGGTCCTTTATTAGAAGAATATGTATTCAGAAAAGTAATCTTTGGAGAATTATTTAATGCGATTAAAGGTAATCGTATCGTAGCATttatt                                                                                                                                      >  2:71871/1‑149 (MQ=255)

        aGCTATTTATTATTGCTCGAAAAATACCTTTATTTATCTTCTTTGTATCTATTATTGGTCCTTTATTAGAAGAATATGTATTCAGAAAAGTAATCTTTGGAGAATTATTTAATGCGATTAAAGGTAATCGTATCGTAGCATTTattatt                                                                                                                                   <  2:968815/149‑1 (MQ=255)

        aGCTAATTATTATTGCTCGAAAAATACCTATATTTATCTTCTTTGTATCTATTATTGGTCCTTTATTAGAAGAATATGTATTCAGAAAAGTAATCTTTGGAGAATTATTTAATGCGATTAAAGGTAATCGTATCGTAGCATTTattatt                                                                                                                                   <  2:1540618/149‑1 (MQ=255)

        aGCTAATTATTATTGCTCGAAAAATACCTATATTTATCTTCTTTGTATCTATTATTGGTCCTTTATTAGAAGAATATGTATTCAGAAAAGTAATCTTTGGAGAATTATTTAATGCGATTAAAGGTAATCGTATCGTAGCATTTattatt                                                                                                                                   <  1:520434/149‑1 (MQ=255)

         gCTAATTATTATTGCTCGAAAAATACCTATATTTATCTTCTTTGTATCTATTATTGGTCCTTTATTAGAAGAATATGTATTCAGAAAAGTAATCTTTGGAGAATTATTTAATGCGATTAAAGGTAATCGTATCGTAGCATTTATTATTg                                                                                                                                  >  2:163024/1‑149 (MQ=255)

              ttattattGCTCGAAAAATACCTATATTTATCTTCTTTGTATCTATTATTGGTCCTTTATTAGAAGAATATGTATTCAGAAAAGTAATCTTTGGAGAATTATTTAATGCGATTAAAGGTAATCGTATCGTAGCATTTATTATTGCTaca                                                                                                                             <  2:290167/149‑1 (MQ=255)

              ttattattGCTCGAAAAATACCTATATTTATCTTCTTTGTATCTATTATTGGTCCTTTATTAGAAGAATATGTATTCAGAAAAGTAATCTTTGGAGAATTATTTAATGCGATTAAAGGTAATCGTATCGTAGCATTTATTATTGCTaca                                                                                                                             <  1:53078/149‑1 (MQ=255)

                attattGCTCGAAAAATACCTATATTTATCTTCTTTGTATCTATTATTGGTCCTTTATTAGAAGAATATGTATTCAGAAAAGTAATCTTTGGAGAATTATTTAATGCGATTAAAGGTAATCGTATCGTAGCATTTATTATTGCTacaac                                                                                                                           <  2:1029312/149‑1 (MQ=255)

                attattGCTCGAAAAATACCTATATTTATCTTCTTTGTATCTATTATTGGTCCTTTATTAGAAGAATATGTATTCAGAAAAGTAATCTTTGGAGAATTATTTAATGCGATTAAAGGTAATCGTATCGTAGCATTTATTATTGCTacaac                                                                                                                           <  2:901912/149‑1 (MQ=255)

                       cacGAAAAATACCTCTATTTAACTTCTTTGTATCTATTATTGGTCCTTTATCAAAAGAATATGTATTCAGAAAAGTAATCTTTGGAGAATTATTTAATGCGATTAAAGGTAATCGTATCGTAGCATTTATTATTGCTACAACAGTAAGt                                                                                                                    <  1:420688/147‑1 (MQ=255)

                        tCGATAAATTCCTATATTTATCTTCGTTGTATCTATTATTGTTCCTTTATTAGAAGAATATGTATTCAGAAAAGTAATCTTTGGAGAATTATTTAATGCGATTAAAGGTAATCGTATCGTAGCATTTATTATTGCTACAACAGTAAGtt                                                                                                                   <  2:1341928/149‑1 (MQ=255)

                        tCGAAAAATACCTATATTTATCTTCTTTGTATCTATTATTGGTCCTTTATTAGAAGAATATGTATTCAGAAAAGTAATCTTTGGAGAATTATTTAATGCGATTAAAGGTAATCGTATCGTAGCATTTATTATTTCTACAACCGTAAGtt                                                                                                                   >  1:924790/1‑149 (MQ=255)

                        tCGAAAAATACCTATATTTATCTTCTTTGTATCTATTATTGGTCCTTTATTAGAAGAATATGTATTCAGAAAAGTAATCTTTGGAGAATTATTTAATGCGATTAAAGGTAATCGTATCGTAGCATTTATTATTGCTACAACAGTAAGtt                                                                                                                   >  1:1032558/1‑149 (MQ=255)

                         cGAAAACTACCTATATTTATCTTCTTTGTATCTATTATTGGTCCTTTATTAGAAGAATATGTATTCAGAAAAGTAATCTTTGGAGAATTATTTAATGCGATTAAAGGTAATCGTATCGTAGCATTTATTATTGCTACAACAGTAAGTTc                                                                                                                  <  2:1199779/149‑1 (MQ=255)

                         cGAAAAATACCTATATTTATCTTCTTTGTATCTATTATTGGTCCTTTATTAGAAGAATATGTATTCAGAAAAGTAATCTTTGGAGAATTATTTAATGCGATTAAAGGTAATCGTATCGTAGCATTTATTATTGCTACACCAGTAAGTTc                                                                                                                  >  2:398660/1‑149 (MQ=255)

                         cGAAAAATACCTATATTTATCTGCTTTATATCTATTATTGGTCCTTTATTAGAAGAATATGTATTCAGAAAAGTAATCTTTGGAGAATTATTTAATGCGATTAAAGGTAATCGTATCGTAGCATTTATTATTGCTACAACAGTAAGtgc                                                                                                                  >  1:948784/1‑147 (MQ=255)

                         agagtaaTACATATATTTATCTTCTTTGTATCTATTATTGGTCCTTTATTAGAAGAATATGTATTCAGAAAAGTAATCTTTGGAGAATTATTTAATGCGCTTAAAGACAATCGTATCGTAGCATATATTATTGCTACAACAGTAAGTTc                                                                                                                  >  2:637541/6‑149 (MQ=255)

                           aaaaaTACCTATATTTATCTTCTTTGTATTTATTATTGGTCCTTTATTAGAAGAATATGTATTCAGAAAAGTAATCTTTGGAGAATTATTTAATGCGATTAAAGGTAATCGTATCGTAGCATTTATTATTGCTCCTAAAGTAAAGTCAt                                                                                                                <  2:447932/149‑1 (MQ=255)

                            aaaaTACCTATATTTATCTTCTTTGTATCTATTATTGGTCCTTTATTAGAAGAATATGTATTCAGAAAAGTAATCTTTGGAGAATTATTTAATGCGATTAAAGGTAATCGTATCGTAGCATTTATTATTGCTACAACAGTAAGTTCAtt                                                                                                               >  1:282509/1‑149 (MQ=255)

                             aaaTACCTATATTTATCTTCTTTGTATCTATTATTGGTCCTTTATTAGAAGAATATGTATTCAGAAAAGTAATCTTTGGAGAATTATTTAATGCGATTAAAGGTAATCGTATCGTAGCATTTATTATTGCTACAACAGTAAGTTCATTa                                                                                                              <  1:679996/149‑1 (MQ=255)

                                   cTATATTTATCTTCTTTGTTTCTATTATTGGTCCTTTATTAGAAGAATATGTATTCAGAAAAGTAATCTTTGGAGAATTATTTAATGCGATTAAAGGTAATCGTATCGTAGCATTTATTATTGCTACAACAGTAAGTTCATTAATAttt                                                                                                        <  2:103335/149‑1 (MQ=255)

                                    tataTTTATCTTCTTTGTATCTATTATTGGTCCTTTATTAGAAGAATATGTATTCAGAAAAGTAATCTTTGGAGAATTATTTAATGCGATTAAAGGTAATCGTATCGTAGCATTTATTATTGCTACAACAGTAAGTTCATTAATATTTg                                                                                                       <  2:68592/149‑1 (MQ=255)

                                    tataTTTATCTTCTTTGTATCGATTATTGGTCCTTTATTAGAAGAATATGTATTCAGAAAAGTAATCTTTGGAGAATTATTTAATGCGATTAAAGGTAATCGTATCGTAGCATTTATTATTGCTACAACAGTAAGTTCATTAATATTTg                                                                                                       <  1:1075225/149‑1 (MQ=255)

                                      tatTTATCTTCTTTGTATCTATTATTGGTCCTTTATTAGAAGAATATGTATTCAGAAAAGTAATCTTTGGAGAATTATTTAATGCGATTAAAGGTAATCGCATCGTAGCATTTATTATTGCTACAACAGTAAGTTCATTAATATTTGCa                                                                                                     <  2:210012/149‑1 (MQ=255)

                                       atTTATCTTCTTTGTATCTATTATTGGTCCTTTATTAGAAGAATATGTATTCAGAAAAGTAATCTTTGGAGAATTATTTAATGCGATTAAAGGTAATCGTATCGTAGCATTTATTATTGCTACAACAGTAAGTTCATTAATATTTGCAt                                                                                                    >  2:110927/1‑149 (MQ=255)

                                       atTTATCTTCTTTGTATCTATTATTGGTCCTTTATTAGAAGAATATGTATTCAGAAAAGTAATCTTTGGAGAATTATTTAATGCGATTAAAGGTAATCGTATCGTAGCATTTATTATTGCTACAACAGTAAGTTCATTAATATTTGCAt                                                                                                    >  1:1128916/1‑149 (MQ=255)

                                         ttATCTTCTTTGTATCTATTATTGGTCCTTTATTAGAAGAATATGTATTCAGAATAGTAATCTTTGGAGAATTATTTAATGCGATTAAAGTTAATCGTATCGTAGCATTTATTATTGCTACAACAGTAAGTTCATTAATCTTTGCATTa                                                                                                  <  2:1271420/149‑1 (MQ=255)

                                          tATCTTCTTTGTATCTATTATTGGTCCTTTATTAGAAGAATATGTATTCAGAAAAGTAATCTTTGGAGAATTATTTAATGCGATTAAAGGTAATCGTATCGTAGCATTTATTATTGCTACAACAGTAAGTTCATTAATATTTGCATTAg                                                                                                 <  2:822486/149‑1 (MQ=255)

                                           aTCTTCTTTTTATCTATTATTGGTCCTTTATTAGAAGAATATGTATTCAGAAAAGTAATCTTTGGAGAATTATTTAATGCGATTAAAGGTAATCGTATCGTAGCATTTATTATTGCTACAACAGTAAGTTCATTAATATTTGCATTAGc                                                                                                <  2:348186/149‑1 (MQ=255)

                                           aTCTTCTTTGTATCTATTATTGGTCCTTTATTAGAAGAATATGTATTCAGAAAAGTAATCTTTGGAGAATTATTTAATGCGATTAAAGGTAATCGTATCGTAGCATTTATTATTGCTACAACAGTAAGTTCATTAATATTTGCATTAGc                                                                                                <  1:1558209/149‑1 (MQ=255)

                                           aTCTTCTTGGTATCTATTATTGGTTCTTTATTAGAAGAATATGTATTCAGAAAAGTAATCTTTGGAGAATTATTTAATGCGATTAAAGGTAATCGTATCGTAGCATTTATTATTGCTACAACAGTAAGTTCATTAATATTTGCATTAGc                                                                                                <  2:725549/149‑1 (MQ=255)

                                               tcttTGTATCTATTATTGGTCCTTTATTAGAAGAATATGTATTCAGAAAAGTAATCTTTGGAGAATTATTTAATGCGATTAAAGGTAATCGTATCGTAGCATTTATTATTGCTACAACAGTAAGTTCATTAATATTTGCATTAGCACAt                                                                                            >  2:617374/1‑149 (MQ=255)

                                                 tttGTATCTATTATTGGTCCTTTATTAGAAGAATATGTATTCAGAAAAGTAATCTTTGGAGAATTATTTAATGCGATTAAAGGTAATCGTATCGTAGCATTTATTATTGCTACAACAGTAAGTTCATTAATATTTGCATTAGCACATaa                                                                                          >  1:909717/1‑149 (MQ=255)

                                                 tttGTATCTATTATTGGTCCTTTATTAGAAGAATATGTATTCAGAAAAGTAATCTTTGGAGAATTATTTAATGCGATTAAAGGTAATCGTATCGTAGCATTTATTATTGCTACAACAGTAAGTTCATTAATATTTGCATTAGCACATaa                                                                                          >  1:1370474/1‑149 (MQ=255)

                                                  ttGTATCTATTATTGGTCCTTTATTAGAAGAATATGTATTCAGAAAAGTAATCTTTGGAGAATTATTTAATGCGATTAAAGGTAATCGTATCGTAGCATTTATTATTGCTACAACAGTAAGTTCATTAATATTTGCATTAGCACATAAt                                                                                         >  1:1358388/1‑149 (MQ=255)

                                                   tGTATCTATTATTGGTCCTTTATTAGAAGAATATGTATTCAGAAAAGTAATCTTTGGAGAATTATTTAATGCGATTAAAGGTAATCGTATCGTAGCATTTATTATTGCTAAAACAGTGAGGTAATTAATATTTGCGTTAGCACAtcagg                                                                                        >  1:620279/1‑145 (MQ=255)

                                                    gTATCTATTATTGGTCCTTTATTAGAAGAATATGTATTCAGAAAAGTAATCTTTGGAGAATTATTTAATGCGATTAAAGGTAATCGTATCGTAGCATTTATTATTGCTACAACAGTAAGTTCATTAATATTTGCATTAGCACATAATGa                                                                                       <  2:1409123/149‑1 (MQ=255)

                                                      aTCTATTATTGGTCCTTTATTAGAAGAATATGTATTCAGAAAAGTAATCTTTGGAGAATTATTTAATGCGATTAAAGGTAATCGTATCGTAGCATTTATTATTGCTACAACAGTAAGTTCATTAATATTTGCATTAGCACATAATGAtt                                                                                     <  2:261092/149‑1 (MQ=255)

                                                       tCTATTATTGGTCCTTTATTAGAAGAATATGTATTCAGAAAAGTAATCTTTGGAGAATTATTTAATGCGATTAAAGGTAATCGTATCGTAGCATTTATTATTGCTACAACAGTAAGTTCATTAATATTTGCATTAGCACATAATGAttt                                                                                    <  2:1160938/149‑1 (MQ=255)

                                                          attattGGTCCTTTATTAGAAGAATATGTATTCAGAAAAGTAATCTTTGGAGAATTATTTAGTGCGATTAAAGGTAATCGTATCGTAGCATTTATTATTGCTACAACAGTAAGTTCCTTAATATTTGCATTAGCACATAATGATTTTaa                                                                                 >  1:1154143/1‑149 (MQ=255)

                                                                ggTCCTTTATTAGAAGAATATGTATTCAGAAAAGTAATCTTTGGAGAATTATTTAATGCGATTAAAGGTAATCGTATCGTAGCATTTATTATTGCTACAACAGTAAGTTCATTAATATTTGCATTAGCACATAATGATTTCAAATTTAt                                                                           >  2:1295422/1‑149 (MQ=255)

                                                                ggTCCTTTATTAGAAGAATATGTATTCAGAAAAGTAATCTTTGGAGAATTATTTAATGCGATTAAAGGTAATCGTATCGTAGCATTTATTATTGCTACAACAGTAAGTTCATTAATATTTGCATTAGCACATAATGATTTCAAATTTAt                                                                           >  2:1185924/1‑149 (MQ=255)

                                                                 gTCCTTTATTAGAAGAATATGTATTCAGAAAAGTAATCTTTGGGGAATTATTTAATGCGATTAAAGGTAATCGTATCGTAGCATTTATTATTGCTACAACAGTAAGTTCATTAATATTTGCATTAGCACATAATGATTTGAAATTTAtt                                                                          >  1:724074/1‑149 (MQ=255)

                                                                 gTCCTTTATTAGAAGAATATGTATTCAGAAAAGTAATCTTTGGAGAATTATTTAATGAGAATAAAGATAATCGTATCGTAGCAATTATCATTGCTAAAATAATAAGTTCATTAGTATTTGCATTAGCTCTTATTGATGTCAATTGTAtt                                                                          >  2:1620066/1‑149 (MQ=255)

                                                                   ccTTTATTAGAAGAATATGTATTCAGAAAAGTAATCTTTGGAGAATTATTTAATGCGATTAAAGGTAATCGTATCGTAGCATTTATTATTGCTACAACAGTAAGTTCATTAATATTTGCATTAGGACATAATGATTTCAACTTGATTcc                                                                        >  1:119777/1‑149 (MQ=255)

                                                                   ccTTTATTAGAAGAATATGTATTAAGAAAAGTAATCTTTGGAGAATTATTTAATGCGATTAAAGGTAATCGTATCGTAGCATTTATTATTGCTACAACAGTAAGTTCATTAATATTTGCATTAGCACATGATGATATCAAATTTATTcc                                                                        >  2:504261/1‑149 (MQ=255)

                                                                          taGAAGAATATGTATTCAGAAAAGTAATCTTTGGAGAATTATTCAATGCGATTAAAGGTAATCGTATCGTAGCATTTATTATTGCTACAACAGTAAGTTCATTAATATTTGCATTAGCACATAATGATTTCAAATTTATTACAGTTCAt                                                                 >  2:172316/1‑149 (MQ=255)

                                                                            gaagaaTATGTATTCAGAAAAGTAATCTTTGGAGAATTATTTAATGCGATTAAAGGTAATCGTATCGTAGCATTTATTATTGCTACAACAGTAAGTTCATTAATCTTTGCATTAGCACATAATGATTTCAAATTTATTCCAGTTTAttt                                                               >  2:1323019/1‑149 (MQ=255)

                                                                             aagaaTATGTATTCAGAAAAGTAATCTTTGGGGAATAATTTAATGCGATTAAAGGTAATAGTATAGTAGGAGGTAGGATTGCTACAACAGTAAGTTAATTAATATTTGCATTAGCACATAATGATTTCAAAGTTATTCCAGTATAtttt                                                              >  2:1123367/1‑149 (MQ=255)

                                                                             aagaaTATGTATTCAGAAAAGTAATCTTTGGAGAATTATTTAATGCGATTAAAGGTAATCGTATCGTAGCATTTATTATTGCTACAACAGTAAGTTCATTAATATTTGCATTAGCACATAATGATTTCAAATTTATTCCAGTTTAtttt                                                              >  2:39415/1‑149 (MQ=255)

                                                                               aaaTATGTATTCAGAAAAGTAATCATTGGAGAATTATTTAATGCGATTAAAGGTAATCGTATCGTAGCATTTATTATTGCTACAACAGTAAGTTCATTAATATTTGCATTAGCACATAATGATTTCAAATTTATTCCAGTTTATTTTgg                                                            <  1:311403/148‑1 (MQ=255)

                                                                                aaTATGTATTCAGAAAAGTAATCTTTGGAGAATTATTTAATGCGATTAAAGGTAATCGTATCGTAGCATTTATTATTGCTACAACAGTAAGTTCATTAATATTTGCATTAGCACATAATGATTTCAAATTTATTCCAGTTTATTTTGGt                                                           >  1:1378586/1‑149 (MQ=255)

                                                                                   atGTATTCAGAAAAGTAATCTTTGGAGAATTATTTAATGCGATTAAAGGTAATCGTATCGTAGCATTTATTATTGCTACAACAGTAAGTTCATTAATATTTGCATTAGCTCATAATGGTTTCAAATTTATTCCAGTTTATTTTGGTCTg                                                        <  1:1356638/149‑1 (MQ=255)

                                                                                      tATTCAGAAAAGTAATCTTTGGAGAAATATTTAATGCGATTAAAGGTAATCGTATCGTAGCATTTATTATTGCTACAACAGAAAGTTCAGTAATATTTGCATTAGCACATAATGATTTCAAATTTATTCCAATTTATTTTGGTATGAgt                                                     >  2:1686094/1‑149 (MQ=255)

                                                                                         tgaGAAAAGTAATCTTTGGAGAATTATTTAATGCGATTAAAGGTAATCGTATCGTAGCATTTATTATTGCTACAACAGTAAGTTCATTAATATTTGCCTTAGCACATAATGATTTCAAATTTATTCCAGTTTATTTTGGTATGGGTGTc                                                  >  2:722350/3‑149 (MQ=255)

                                                                                         tccgaccaGTAATCTTTGGAGAATTATGTAATGCGATTAAAGGTAATCGTATCGTAGCATTTATTATTGCTACAACAGTAAGTTCATTAATATTTGCATTAGCACATAATGATTTCAAATTTATTCCAGTTTATTTTGGTATGGGTGTc                                                  >  2:1140945/8‑149 (MQ=255)

                                                                                         tCAGAAAAGTAATCTTTGGAGAATTATTTAATGCGATTAAAGGTAATCGTATCGTAGCATTTATTATTGCTACAACAGTAAGTTCATTAATATTTGCATTAGCACATAATGATTTCAAATTTATTCCAGTTTATTTTGGTATGGGTGTc                                                  >  1:597075/1‑149 (MQ=255)

                                                                                           aGAAAAGTAATCTTTGGAGAATTATTTAATGCGATTAAAGGTAATCGTATCGTAGCATTTATTATTGCTACAACAGTAAGTTCATTAATATTTGCATTAGCACATAATGATTTCAAATTTATTCCAGTTTATTTTGGTATGGGTGTCAt                                                >  1:1639605/1‑149 (MQ=255)

                                                                                            gAAAAGTAATCTTTGGAGAATTATTTAATGCGATTAAAGGTAATCGTATCGTAGCATTTATTATTGCTACAACAGTAAGTTCATTAATATTTGCATTAGCACATAATGATTTAAAATTTATTCCAGTTTATTTTGGTATGGGTGTCAtt                                               >  2:317199/1‑149 (MQ=255)

                                                                                               aaGGAATCTTTGGAGAATTATTTAATGCGATTAAAGGTAATCGTATCGTAGCATTTATTATTGCTACAACAGTAAGTTCATTAATATTTGCATTAGCACATAATGATTTCAAATTTATTCCAGTTTATTTTGGTATGGGTGTCAttttt                                            <  1:379401/149‑1 (MQ=255)

                                                                                                        ttGGAGAATTATTTAATGCGATTAAAGGTAATCGTATCGTAGCATTTATTATTGCTACAACAGTAAGTTCATTAATATTTGCATTAGCACATAATGATTTCAAATTTATTCCAGTTTATTTTGGTATGGGTGTCATTTTTTCCTTAGCa                                   >  1:330921/1‑149 (MQ=255)

                                                                                                             gaATTATTTAATGCGATTAAAGGTAATCGTATCGTAGCATTTATTATTGCTACAACAGTAAGTTCATTAATATTTGCATTAGCACATAATGATTTCAAATTTATTCCAGTTTATTTTGGTATGGGTGTCATTTTTTCATTAGCATATGt                              >  2:507101/1‑149 (MQ=255)

                                                                                                              aaTTATTTAATGCGATTAAAGGTAATCGTATCGTAGCATTTATTATTGCTACAACAGTAAGTTCATTAATATTTGCATTAGCACATAATGATTTCAAATTTATTCCAGTTTATTTTGGTATGGGTGTCATTTTTTCATTAGCATATGtt                             <  1:154595/149‑1 (MQ=255)

                                                                                                              aaTTATTTAATGCGAGTAAAGGTAATCGTATCGTAGCATTTATTATTGCTACAACAGTAAGTTCATTAATATTTGCATTAGCACATAATGATTTCAAATTTATTCCAGTTTATTTTGGTATGGGTGTCATTTTTTCATTAGCATATGtt                             <  2:455908/149‑1 (MQ=255)

                                                                                                                 tattTAATGCGATTAAAGGTAATCGTATCGTAGCATTTATTATTGCTACAACAGTAAGTTCATTAATATTTGCATTAGCACATAATGATTTCAAATTTATTCCAGTTTATTTTGGTATGGGTGTCATTTTTTCATTAGCATATGTTTgg                          <  2:815188/149‑1 (MQ=255)

                                                                                                                  attTAATGCGATTAAAGGTAATCGTATCGTAGCATTTATTATTGCTACAACAGTAAGTTCATTAATATTTGCATTAGCACATAATGATTTCAAATTTATTCCAGTTTATTTTGGTATGGGTGTCATTTTTTCATTAGCATATGTTTGGa                         >  2:980296/1‑149 (MQ=255)

                                                                                                                    ttAATGCGATTAAAGGTAATCGTATCGTAGCATTTATTATTGCTACAACAGTAAGTTCATTAATATTTGCATTAGCACATAATGATTTCAAATTTATTCCAGTTTATTTTGGTATGGGTGTCATTTTTTCATTTGCATATGTTTGGACa                       >  2:1652431/1‑149 (MQ=255)

                                                                                                                         gccaTTAAAGGTAATCGTATCGTAGCATTTATTATTGCTACAACAGTAAGTTCATTAATATTTGCATTAGCACATAATGATTTCAAATTTATTCCAGTTTATTTTGGTATGGGTGTCATTTTTTCATTAGCATATGTTTGGACAAAACg                  <  1:937264/146‑1 (MQ=255)

                                                                                                                         gCGATTAAAGGTAATCGTATCGTAGCATTTATTATTGCTACAACAGTAAGTTCATTAATATTTGCATTAGCACATAATGATTTCAAATTTATTCCAGTTTATTTTGGTATGGGTGTCATTTTTTTATTATCATATGTTTGGACAAAACg                  >  2:1635705/1‑149 (MQ=255)

                                                                                                                         gCGATTAAAGGTAATCGTATCGTAGCATTTATTATTGCTACAACAGTAAGTTCATTAATATTTGCATTAGCACATAATGATTTCAAATTTATTCCAGTTTATTTTGGTATGGGTGTCATTTTTTCATTAGCATATGTTTGGACAAAACg                  <  1:687916/149‑1 (MQ=255)

                                                                                                                         gCGATTAAAGGTAATCGTATCGTAGCATTTATTATTGCTACAACAGTAAGTTCATTAATATTTGCATTAGCACATAATGATTTCAAATTTATTCCAGTTTATTTTGGTATGGGTGTCATTTTTTCATTAGCATATGTTTGGACAAAACg                  >  2:1300670/1‑149 (MQ=255)

                                                                                                                          cGATTACAGGTAATCGTATCGTAGCATTTATTATTGCTACAACAGTAAGTTCATTAATATTTGCATTAGCACATAATGATTTCAAATTTATTCCAGTTTATTTTGGTATGGGTGTCATTTTTTCATTAGCATATGTTTGGACAAAACgg                 <  1:370413/149‑1 (MQ=255)

                                                                                                                          cGATTAAAGGTAATCGTATCGTAGCATTTATTATTGCTCCTCCATTAAGTTCATTAATATTTGCATAAGCACATAATGATTTCAAATTTATTCCAGTTTATTTTGGTATGGGTGTCATTTTTTCATTAGCATATGTTTGGACAAAACgg                 >  2:1213956/1‑149 (MQ=255)

                                                                                                                          cGATTAAAGGTAATCGTATCGTAGCATTTATTATTGCTACAACAGTAAGTTCATTAATATTTGCATTAGCACATAATGATTTCAAATTTATTCCAGTTTATTTTGGTATGGGTGTCATTTTTTCATTAGCATATGTTTGGACAAAACgg                 >  1:85409/1‑149 (MQ=255)

                                                                                                                                aaGGTAATCGTATCGTAGCATTTATTATTGCTACAACAGTAAGTTCATTAATATTTGCATTAGCACATAATGATTTCAAATTTATTCCAGTTTATTTTGGTATGGGTGTCATTTTTTCTTTATCATTTGTTTGGACAAAGCGGCTTTCt           >  2:58003/1‑149 (MQ=255)

                                                                                                                                aaGGTAATCGTATCGTAGCATTTATTATTGCTACAACAGTAAGTTCATTAATATTTGCATTAGCACATAATGATTTCAAATTTATTCCAGTTTATTTTGGTATGGGTGTCATTTTTTCATTAGCATATGTTTTGACAAAACGGCTTGCt           >  2:679183/1‑149 (MQ=255)

                                                                                                                                aaGGTAATCGTATCGTAGCATTTATTATTGCTACAACAGTAAGTTCATTAATATTTGCAGTAGCACATAATGATTTCAAATTTATTCCAGTTTATTTTGGTATGGGTGTCATTTTTTCATTAGCATATGTTTGGACAAAACGGCTTGCt           >  1:909716/1‑149 (MQ=255)

                                                                                                                                   ggaaTCGTTTCGTAGCATTTATTATTGCCACAACAGTAAGGTCGTTAATATTTGCATTAGCACATAATGGTTTCAAATTTATTCCAGTTTAGTTTGGTATGGGTGTCATTTTTTCATTAGCATATGTTTGGACAAAACGGCTTGCTGtt        <  2:1199070/147‑1 (MQ=255)

                                                                                                                                   gTAATCGTATCGTAGCATTTATTATTGCTACAACAGTAAGTTCATTAATATTTGCATTAGCACATAATGATTTCAAATTTATTCCAGTTTATTTTGGTATGGGTGTCATTTTTTCATTAGCATATGTTTGGACAAAACGGCTTGCTGtt        <  2:282509/149‑1 (MQ=255)

                                                                                                                                   gTAAGCTTATCGTAGCATTTATTATTGCTACAACAGTAAGTTCATTAATATTTGCTTTTTCTCATAATGATTTCAAATTTATTCCAGTTTATTTTGGTATGGGTGTCATTTTTTCATTAGCATATGTTTGGACAAAACGGCTTGCTGtt        <  1:397101/149‑1 (MQ=255)

                                                                                                                                    tAATCGTATCGTAGCATTTATTAGTGCTACATCAGTAAGTTCATTAATATTTGCATTAGCACATAATGATTTCAAATTTATTCCAGTTTATTTTGGTATGGGTGTCATTTTTTCATTAGCATATGTTTGGACAAAACGGCTTGCTGTTc       <  1:107820/149‑1 (MQ=255)

                                                                                                                                    tAATAGTATCGTAGAATTTATTATTGCTACATCAGTAAGTTCATTAATATTTGCATTAGCACATAATGATTTCAAATTTATTCCAGATTATTTTGGTATGGGGGTTATCTTTTCATTAGCATATGTTTGGACAAAACGGCTTGCTGTTc       <  1:258053/149‑1 (MQ=255)

                                                                                                                                    tAAGCGTATTGTATCATTGATTATTGCGACAACAGTAAGTTCATTAATATTTGCATTAGCACATAATGATTTCAAATTTATTCCAGTTTATTTTGGTATGGGGGTCATTTTTTCATTAGCATATGTTTGGACAAAACGGCTTGCTGTTc       <  1:163024/149‑1 (MQ=255)

                                                                                                                                      atcgtatcgtAGCATTTATTATTGCTACAACAGTAAGTTCATTAATATTTGCATTAGCACATAATGATTTCAAATTTATTCCAGTTTATTTTGGTATGGGTGTCATTTTTTCATTAGCATATGTTTGGACAAAACGGCTTGCTGTTCCa     <  1:116274/149‑1 (MQ=255)

                                                                                                                                      atcgtatcgtAGCATTTATTATTGCTACAACAGTAAGTTCATTAATATTTGCATTAGCACATAATGATTTCAAATTTATTCCAGTTTATTTTGGTATGGGTGTCATTTTTTCATTAGCATATGTTTGGACAAAACGGCTTGCTGTTCCa     <  1:612127/149‑1 (MQ=255)

                                                                                                                                       ccgtctcgtAGCATTTATTATGGCTAAAACAGTAAGTTACTCACTCTTTGCCTTGAGTCAAAAGAAGGTCAAATTTATTCCAGTCTAGTGGGGCATGGGTGTCATTTTTTCATTAGCATATGTTTGGACAAAACGGCTTGCTGTTCCaa    <  2:1648748/148‑1 (MQ=255)

                                                                                                                                         gtatcgtAGCATTTATTATTGCTACAACAGTAAGTTCATTAATATTTGCATTAGCACATAATGATTTCAAATTTATTCCAGTTTATTTTGGTATGGGTGTCATTTTTTCATTAGCATATGTTTGGACAAAACGGCTTGCTGTTCCAatt  >  1:108365/1‑149 (MQ=255)

                                                                                                                                         gtatcgtAGCATTTATTATTGCTACAACAGTAAGTTCATTAATATTTGCATTAGCACATAATGATTTCAAATTTATTCCAGTTTATTTTGGTATGGGTGTCATTTTTTCATTAGCATATGTTTGGACAAAACGGCTTGCTGTTCAAatt  >  2:520229/1‑149 (MQ=255)

TACAGAAAAGCTAATTATTATTGCTCGAAAAATACCTATATTTATCTTCTTTGTATCTATTATTGGTCCTTTATTAGAAGAATATGTATTCAGAAAAGTAATCTTTGGAGAATTATTTAATGCGATTAAAGGTAATCGTATCGTGGCATTTATTATTGCTACAACAGTAAGTTCATTAATATTTGCATTAGCACATAATGATTTCAAATTTATTCCAGTTTATTTTGGTATGGGTGTCATTTTTTCATTAGCATATGTTTGGACAAAACGGCTTGCTGTTCCAATT  >  USA300TCH1516_ALE/2142966‑2143251

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`