Predicted mutation
evidence seq id position mutation annotation gene description
RA USA300TCH1516_ALE 479,553 G→A A194T (GCA→ACA)  USA300HOU_RS02280 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE479,5530GA100.0% 705.2 / NA 215A194T (GCA→ACA) USA300HOU_RS02280hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (96/119);  total (96/119)

Only 100 of 218 total aligned reads displayed.

CCGATGAGTGATGCAATAATAGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATGCATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAGATCAGGAAGACTACCAAGCTTATGT  >  USA300TCH1516_ALE/479409‑479698
                                                                                                                                                 |                                                                                                                                                 
ccGATGAGTGATGCAATAATAGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACcatacatg                                                                                                                                               >  1:1470097/1‑149 (MQ=255)
  gATGAGTGATGCAATAATAGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGt                                                                                                                                             >  2:601710/1‑149 (MQ=255)
    tGAGTGATGCAATAATAGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTg                                                                                                                                           <  2:1027480/149‑1 (MQ=255)
    tGAGTGATGCAATAATAGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTg                                                                                                                                           >  1:305732/1‑149 (MQ=255)
     gAGTGATGCAATAATAGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCCAGTTGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGc                                                                                                                                          <  2:1047682/149‑1 (MQ=255)
       gggATGCAATAATAGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCAt                                                                                                                                        <  2:36854/147‑1 (MQ=255)
              aataatAGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAgcgc                                                                                                                                 <  1:1004063/149‑1 (MQ=255)
               ataataGGTGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCa                                                                                                                                <  2:229599/149‑1 (MQ=255)
               ataataGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCa                                                                                                                                >  1:380461/1‑149 (MQ=255)
               ataataGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGG‑CGCTGTTGCAAGCGTGAGCAAAGTTTTTTCCATACATGGTTGCATTTAGCGCa                                                                                                                                >  1:1680475/1‑149 (MQ=255)
                  ataGATGAACAAGGTGATCCACTTAGCGAACAAGTGAATCGTCAAATGAGTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATGTAGCCGAACa                                                                                                                             >  1:1261189/1‑149 (MQ=255)
                  ataGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACa                                                                                                                             >  1:809779/1‑149 (MQ=255)
                  ataGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACATTATATCGATCAATTTCTATCGAGTTGGAAACAACAT‑AAGCGGGAGCACAGTTTTTACCATACATGGTTGCATTTAGCGCAACa                                                                                                                             >  1:392276/1‑149 (MQ=255)
                     gATGAACATGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGa                                                                                                                          <  1:1718085/149‑1 (MQ=255)
                     gATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGa                                                                                                                          >  2:190310/1‑149 (MQ=255)
                      aTGAACAAGGTGAGCCACTTAGCGAACACGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGAc                                                                                                                         <  1:1196983/149‑1 (MQ=255)
                      aTGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACCTGGTTGCATTTAGCGCAACATGAc                                                                                                                         >  1:76772/1‑149 (MQ=255)
                      aTGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGAc                                                                                                                         >  1:1093073/1‑149 (MQ=255)
                      aTGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGAc                                                                                                                         <  2:1513526/149‑1 (MQ=255)
                      aTGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATAAATTTCTATCGAGTTGGACAATGCCG‑AAGCGTGAGCAAANTTTTTACCATACATGGTTGCATTTACCGCAACACGAc                                                                                                                         >  2:133457/1‑149 (MQ=255)
                       tcaaCAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTAGATCGATCAATTTCTATCGAGTTGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGAcc                                                                                                                        <  2:1556545/147‑1 (MQ=255)
                       tGAACAAGGTGAGCCACTTAGCGAACAAGTGAACCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGAcc                                                                                                                        <  2:111470/149‑1 (MQ=255)
                       ggAACGAGGCGAGCCACTTAGCGAACAAGTGACTCGTCGAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGAcc                                                                                                                        <  2:1194987/148‑1 (MQ=255)
                         aaCAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAGCATGACCAt                                                                                                                      <  2:631561/149‑1 (MQ=255)
                          aCAAGGGGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATa                                                                                                                     <  1:856694/149‑1 (MQ=255)
                             aGGTGAGCCACTTAGCGAACAAGTGAATCGTTAAATGATAAACTGGCAGAATGTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTACCGCAACATGACCATAGtt                                                                                                                  <  1:1244664/149‑1 (MQ=255)
                                ggAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTa                                                                                                               <  2:1381677/148‑1 (MQ=255)
                                  ggCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATCGTTGCATTTAGCGCAACATGACCATAGTTTTACt                                                                                                             <  2:1068526/148‑1 (MQ=255)
                                  aGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACt                                                                                                             >  1:1351088/1‑149 (MQ=255)
                                   tccACTTAGCGAACAAGTGAAGCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCG‑AATCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTa                                                                                                            <  2:1050460/148‑1 (MQ=255)
                                   gCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTa                                                                                                            >  1:180729/1‑149 (MQ=255)
                                   gCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTa                                                                                                            >  2:457284/1‑149 (MQ=255)
                                    ccACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTCAGCGCAACATGACCATAGTTTTACTaa                                                                                                           <  1:1120002/149‑1 (MQ=255)
                                    caaCTTAGCGAACAAGTCATTCCTCAAATTCTTAAATGGACGAAAATTTACATAGATCAATTTCTATCGAGTTGGTCACTGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTaa                                                                                                           <  1:104258/147‑1 (MQ=255)
                                           gCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAg                                                                                                    <  1:971936/149‑1 (MQ=255)
                                            cGAACAAGTGAATCGTCAAATGATTAAATGGACGAAGCTTTATATCGATCAATTGCTATCGAGTGGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACAGGACCATAGCATTACTGAAGaacaaa                                                                                                   >  2:1611600/1‑147 (MQ=255)
                                            cGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAgc                                                                                                   >  2:937696/1‑149 (MQ=255)
                                            cGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAgc                                                                                                   >  1:231484/1‑149 (MQ=255)
                                            cGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAgc                                                                                                   >  2:1016738/1‑149 (MQ=255)
                                               aCAAGTGAATCGTCAAATGATTAAATGTACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGcc                                                                                                >  1:1007022/1‑149 (MQ=255)
                                               aCAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGcc                                                                                                >  1:466660/1‑149 (MQ=255)
                                                cAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCa                                                                                               <  1:1318546/149‑1 (MQ=255)
                                                  aGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAg                                                                                             >  1:286653/1‑149 (MQ=255)
                                                    tGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTg                                                                                           >  2:1275061/1‑149 (MQ=255)
                                                    tGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTCTTACTAAAGCACAGCGCCAAGTg                                                                                           <  1:866261/149‑1 (MQ=255)
                                                    gaaaTCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTg                                                                                           <  1:602082/147‑1 (MQ=255)
                                                     gAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGa                                                                                          <  2:1093588/149‑1 (MQ=255)
                                                     gAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATAAATTTCTATCGAGTTGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAgggc                                                                                          >  2:1457074/1‑146 (MQ=255)
                                                     gAATCGTCAAATGACTAAATGGACGAAACTGTATATCGATCAATTGCTATCGAGTTGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGa                                                                                          <  2:1360218/149‑1 (MQ=255)
                                                      aaTCGTCAAATGATTAAATGGACGAAAGTTTATATCGATCAAGTCCTATCGAGTTGGACAATGCCG‑AAGCGTGAGCTAAGTTTTTTCCATACATCGTTGCATGTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGAt                                                                                         <  2:830652/149‑1 (MQ=255)
                                                      aaTCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAAGATGACCATAGTTTGACTAAAGCACAGCGCCAAGTGAt                                                                                         <  2:65926/149‑1 (MQ=255)
                                                      aaTCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGAt                                                                                         <  2:263635/149‑1 (MQ=255)
                                                          ctCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTaaa                                                                                     <  1:1523057/148‑1 (MQ=255)
                                                           tCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAg                                                                                    <  1:1398973/149‑1 (MQ=255)
                                                           tCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACGAAAGCACAGCGCCAAGTGATTAAAg                                                                                    >  1:32613/1‑149 (MQ=255)
                                                            cAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCAGACAGGGTTGCATTTAGCGCAACATGACCATAGGTGTACTCAAGCGCAGCGCCAAGTGATAAAAgg                                                                                   >  1:681541/1‑149 (MQ=255)
                                                                   ttAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTAccc                                                                            >  1:612608/1‑149 (MQ=255)
                                                                         ggACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGAt                                                                      <  1:190310/149‑1 (MQ=255)
                                                                            cGAAACTTTAAATCGATCAATTTCTATCGAGTTGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCt                                                                   <  2:1144085/149‑1 (MQ=255)
                                                                             gAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCAG‑AAGCGTGAGATGAGTTATTAACATACATGGTTGCATTTAGCGAAGCATGAGCATAGTTTTACGAAATCATGGCGACAAGTGATTTCACGCCTAGCCACTGAGCCta                                                                  >  2:903260/1‑148 (MQ=255)
                                                                                    tataTCGATCAATTTCTATCGAGTTGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGAc                                                           >  1:432709/1‑149 (MQ=255)
                                                                                       atCGATCAATTTCTATCGAGTTGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGAt                                                        <  1:1239558/149‑1 (MQ=255)
                                                                                         cGATCAATTTCTATCGAGTTGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATag                                                      >  2:1232591/1‑149 (MQ=255)
                                                                                         cGATCAATTTCTATCGAGTTGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATACTGAAATGACGATag                                                      >  2:1427534/1‑149 (MQ=255)
                                                                                          gagcAATGTCTATCGAGTTGGACAATGCCG‑AAGCGTGAGCAAAGTTCTAACCATACATGCGTTCATCTAGCGCAACACGACCATAGTGTTACTAAAGCACAGCGCCAAGTGATGAAAGGCTTACCCAATGATCCTGAAATGACGATaga                                                     <  2:529401/146‑1 (MQ=255)
                                                                                          gATCAATTTCTATCGAGTTGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATaga                                                     <  1:315250/149‑1 (MQ=255)
                                                                                           aTCAATTTCTATCGAGTTGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATagag                                                    >  1:1654954/1‑149 (MQ=255)
                                                                                            tCAATTTCTATCGAGTTGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCCTTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGt                                                   <  2:1470097/149‑1 (MQ=255)
                                                                                            tCAAATTCTATCGAGTTGGACAATTCCG‑AAGCCTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTGACCCAATGATCCTGAAATGACGATACAGt                                                   <  2:800263/149‑1 (MQ=255)
                                                                                             cAATTTCTATCGAGTTGGGCAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCATAGCGCCAAGTGATTAAAGGCTTATCCAATGATCCTGAAATGACGATAGAGTc                                                  <  2:942084/149‑1 (MQ=255)
                                                                                             cAATTTCTATCGAGTTGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTc                                                  <  1:934234/149‑1 (MQ=255)
                                                                                             cAATTTCTATCGAGTTGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTc                                                  <  2:1406272/149‑1 (MQ=255)
                                                                                             cAATTTCTATCGAGTTGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGAGAGATTc                                                  <  2:1576824/149‑1 (MQ=255)
                                                                                                 ttCTATCGAGTTGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCGTTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTa                                              >  1:908156/1‑149 (MQ=255)
                                                                                                 ttCTATCGAGTTGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGATTACCCAATGATCCTGAAATGACGATAGAGTCAGTa                                              >  2:339673/1‑149 (MQ=255)
                                                                                                 ttCTATCGAGTAGGACAATGCTG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTACATTTAGCGCAACATGACCATAGTTTTCTTAAAGCACAGCGCCTAGTGATTAAAGGCTTACCCAATAATCCTAGAATGACGATAGAGtaggtc                                              >  2:452728/1‑144 (MQ=255)
                                                                                                 ttCTAGTGAGTTGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTa                                              <  1:336243/149‑1 (MQ=255)
                                                                                                  tCTATCGAGTTGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTAt                                             <  1:744846/149‑1 (MQ=255)
                                                                                                    tATCGGGTTGGACAATGCCG‑AAGCGTTTGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGGTTAAAGGTTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTa                                           <  2:1638458/149‑1 (MQ=255)
                                                                                                       cGAGTTGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAAcc                                        >  2:1523122/1‑148 (MQ=255)
                                                                                                        gAGTTGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTGTTAACTc                                       >  1:795653/1‑149 (MQ=255)
                                                                                                         aGTTGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTACTAACTCa                                      >  1:552630/1‑149 (MQ=255)
                                                                                                          gTTGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCAt                                     >  1:637450/1‑149 (MQ=255)
                                                                                                          gTTGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGAGTAAAGGCTTACCCAATGGTCCTGAAAGGACGATAGAGCCAGTATTAACCCAt                                     >  1:500715/1‑149 (MQ=255)
                                                                                                           ttGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCAtt                                    >  1:1282882/1‑149 (MQ=255)
                                                                                                             ggACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACCTGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCAtttt                                  <  1:1405576/149‑1 (MQ=255)
                                                                                                              gACAATGTCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCAttttt                                 <  1:712143/149‑1 (MQ=255)
                                                                                                              gACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCAttttt                                 <  1:898213/149‑1 (MQ=255)
                                                                                                               aCAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTc                                >  1:823904/1‑149 (MQ=255)
                                                                                                               aCAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTc                                <  2:502060/149‑1 (MQ=255)
                                                                                                                cAATGCCT‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCa                               <  2:410244/149‑1 (MQ=255)
                                                                                                                cAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCa                               <  2:616611/149‑1 (MQ=255)
                                                                                                                cAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCa                               <  2:1646058/149‑1 (MQ=255)
                                                                                                                             gTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAGATCAGGaa                   >  1:582041/1‑149 (MQ=255)
                                                                                                                                   aaaGTTTTTACCATACATGGTTGCATTTACCGCATCATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGGTCCTGAACTGACGATAGAGTCAGTATTAACTCATTGTTCAATAGATCAGGAAGATTAc             >  2:1659384/1‑149 (MQ=255)
                                                                                                                                       tttttACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAGATCAGGAAGACTACCAAg         <  2:888564/149‑1 (MQ=255)
                                                                                                                                       tttttACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAGATCAGGAAGACTACCAAg         <  2:1689167/149‑1 (MQ=255)
                                                                                                                                           tACCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAGATCAGAAAGACTACCGAGCTTa     >  2:565521/1‑149 (MQ=255)
                                                                                                                                            aCCATACATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAATGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAGATGAGGAAGACTACCAAGCTTAt    >  1:1272449/1‑149 (MQ=255)
                                                                                                                                              catacatgGTTGCATTTAGCGCAACATGACCATAGTTTTACAAAAGCACAGCGCCAAGTGATTAAAAGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTACGAACTCATTTGTCAATAGATCAGGAAGACAACCAAGCCTATGt  >  2:867062/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
CCGATGAGTGATGCAATAATAGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCG‑AAGCGTGAGCAAAGTTTTTACCATGCATGGTTGCATTTAGCGCAACATGACCATAGTTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAGATCAGGAAGACTACCAAGCTTATGT  >  USA300TCH1516_ALE/479409‑479698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑