Predicted mutation
evidence seq id position mutation annotation gene description
RA USA300TCH1516_ALE 622,314 C→A S959R (AGC→AGA sdrE → Serine‑aspartate repeat‑containing protein E

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE622,3140CA97.7% 377.6 / NA 132S959R (AGC→AGAsdrESerine‑aspartate repeat‑containing protein E
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base A (81/47);  total (84/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 183 total aligned reads displayed.

TAAAGATGCAGATGGTGGCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGC  >  USA300TCH1516_ALE/622170‑622458
                                                                                                                                                |                                                                                                                                                
tAAAGATGCAGATGGTGGCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTgactcagacagcgactcagactcagacagagact                                                                                                                                              <  1:787230/149‑1 (MQ=255)
     aTGCAGATGGTGGCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACtcagacagcgactcagactcagacagagactccgac                                                                                                                                         >  2:1630208/1‑149 (MQ=255)
     aTGCAGATGGTGGCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACtcagacagcgactcagactcagacagagactcagac                                                                                                                                         <  1:1012697/149‑1 (MQ=255)
        cAGATGGTGGCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCagacagcgactcagactcagacagagactcagcctcc                                                                                                                                      >  1:160185/1‑148 (MQ=255)
        cAGATGGTGGCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAgacagcgactcagactcagacagagactcagactca                                                                                                                                      >  2:131897/1‑149 (MQ=255)
              gtggCGAAGTTGACGTAACAATTAAGGATCAAGATTATTTCACACTTGATAACGGATACATCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGt                                                                                                                                >  2:1222023/1‑149 (MQ=37)
                                cAATTACGGATCATGATGATTTCACACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGCTTCAGACGCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGc                                                                                                              >  2:1471930/1‑149 (MQ=255)
                                cAATTACGGATCATGATGATTTCACACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGCGACTCAGACAGCGACACAGACTCAGACAGAGACTCAGACTCAGACAGTGACTCAGACTCAGACAGc                                                                                                              >  2:697777/1‑149 (MQ=25)
                                 aaTTACGGATCATGATGATTTCACACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCg                                                                                                             >  1:1122925/1‑149 (MQ=255)
                                  atgaCGGATCATGATGATTGCACACTTGAGAACGGATACTTAGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGa                                                                                                            <  2:1193306/146‑1 (MQ=25)
                                  aTTACGGATCATGATGATTTCACACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGa                                                                                                            >  1:266596/1‑149 (MQ=255)
                                   ttACGGATCATGATGATTTCACACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGAc                                                                                                           >  2:749445/1‑149 (MQ=255)
                                    tACGGATCATGATGATTTCACACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACt                                                                                                          <  1:549253/149‑1 (MQ=255)
                                     aCGGATCATGATGATTTCACACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACtc                                                                                                         >  1:796519/1‑149 (MQ=255)
                                     aCGGATCATGATGATTTCACACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACtc                                                                                                         >  1:1391035/1‑149 (MQ=255)
                                      cGGATCATGATGATTTCACACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGCCTCAGACAGAGACTCAGACTCAGACAGGGATTCAGATTCAGACAGCGACtaa                                                                                                        >  1:133352/1‑147 (MQ=255)
                                      cGGATCATGATGATTTCACACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACtca                                                                                                        >  1:1702074/1‑149 (MQ=255)
                                       ggATCATGATGATTTCACACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGCCTCAGACAGTGATTCGAATTCAGAGAGCGACacag                                                                                                       >  2:393539/1‑149 (MQ=37)
                                       ggATCATGATGATTTCACACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACtcag                                                                                                       >  1:1527848/1‑149 (MQ=255)
                                       ggATCATGATGATTTCACACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACtcag                                                                                                       >  1:44869/1‑149 (MQ=255)
                                       ggATCATGATGATTTCACACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGAAAGCGACtcag                                                                                                       >  1:528896/1‑149 (MQ=255)
                                       ggATCATGATGATTTCACACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGAAAGTGATTCGGATTCCGACAGCGACtcag                                                                                                       >  1:676775/1‑149 (MQ=255)
                                         aTCATGATGATTTCACACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACtcagat                                                                                                     >  1:1653353/1‑149 (MQ=255)
                                          ccATGATGATTTCCCACTCGCAAACGGCTATTTCGAAGTAGTTTCAGCTGATCGCGAGTCAGTCTGTGATAGTTACCCAGTCAGCGACTCGGACTCAGACGGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACtcagatt                                                                                                    <  2:49674/148‑1 (MQ=12)
                                             tgatgaTTTCACACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTAAGACCCAGACAGTGATTCAGATTCCGACAGCGGCtcagattcag                                                                                                 >  2:1442092/1‑149 (MQ=255)
                                             tgatgaTTTCACACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACtcagattcag                                                                                                 >  1:1305892/1‑149 (MQ=255)
                                             tgatgaTTTCACACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACtcagagtcag                                                                                                 >  2:519299/1‑149 (MQ=255)
                                             tgatgaGTTCGCACGTGATAACGGGTACTTCGACGAAGATACATCAGACAGCGAGTCAGACTCCGATAGTGTCTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACtcagattcag                                                                                                 <  2:129394/149‑1 (MQ=21)
                                                  atTTCACACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGc                                                                                            >  1:990758/1‑149 (MQ=255)
                                                   tttCCCACCGGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCg                                                                                           <  1:349829/149‑1 (MQ=255)
                                                   tttCACACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCg                                                                                           <  1:954030/149‑1 (MQ=255)
                                                   tgtCACACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCg                                                                                           <  2:1109387/147‑1 (MQ=255)
                                                   tatCACACTTGATAACGGAGACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCg                                                                                           <  2:890167/147‑1 (MQ=255)
                                                     tCACACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAAATAGCGAc                                                                                         >  1:1585488/1‑149 (MQ=255)
                                                      caccCTTGATAACTGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACt                                                                                        <  1:810618/149‑1 (MQ=255)
                                                                 gcGGGTACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGAGAGTGACTCAGACAGCGACTACGACTCAGACAGAGACTCAGAGTCAGACAGGGATTTAGATTAAGACAGCGACTCGGATTCAGATAGCGACTCGGAGTCggcg                                                                             >  2:453883/2‑147 (MQ=25)
                                                                  cGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGGCAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACa                                                                            >  2:1694217/1‑149 (MQ=255)
                                                                  cGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACa                                                                            >  2:42505/1‑149 (MQ=255)
                                                                  cGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACCCAGACTCAGACAGAGACACAGACACAGACAGTGATTCAGACTCAGACCGAGCCACAGAATCCGAGAGAGACTCTGATTCGGAcc                                                                            >  2:1371356/1‑148 (MQ=25)
                                                                   ggATACTTCGAAGAAGACACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAg                                                                           >  1:753429/1‑149 (MQ=255)
                                                                         ttCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTc                                                                     >  1:345082/1‑149 (MQ=255)
                                                                          tCGAAGAAGATACATCAGACATCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCa                                                                    <  1:91186/149‑1 (MQ=255)
                                                                          tCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGGCAGCGATTCa                                                                    >  2:918500/1‑149 (MQ=255)
                                                                          tCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTcc                                                                    >  1:607127/1‑148 (MQ=255)
                                                                          tCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCa                                                                    <  2:1110444/149‑1 (MQ=255)
                                                                          tCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCa                                                                    >  2:218804/1‑149 (MQ=255)
                                                                          tCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCa                                                                    <  2:257909/149‑1 (MQ=255)
                                                                          tCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCa                                                                    >  1:274108/1‑149 (MQ=255)
                                                                          tCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCa                                                                    >  1:1563091/1‑149 (MQ=255)
                                                                           cGAAGAAGATACATCAGACGGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGCGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAg                                                                   <  2:1313085/149‑1 (MQ=255)
                                                                           cGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAg                                                                   >  1:192443/1‑149 (MQ=255)
                                                                           cGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAg                                                                   <  1:1402046/149‑1 (MQ=255)
                                                                           cGAAGAAGATACATCAGACAGCAATTCTGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAg                                                                   >  1:949773/1‑149 (MQ=255)
                                                                             aagaagATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACACAGATTCAGACAGCGAGTCAGAc                                                                 >  2:1004547/1‑149 (MQ=255)
                                                                              agaagtTACACCAGCCCCCGATACAGACACCAATAGTGACTCAGACAGCGACTCAGACTCCGACAGAGACACAGACTCAGACAGTGATTCAGAGTCAGACAGCGACTCAGATTCAGATAGCGACGCAGATTCGGACTGCGATTCAGACt                                                                <  2:803305/149‑1 (MQ=17)
                                                                              agaagaTACATCAGACAGCGATGCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACt                                                                <  2:647885/149‑1 (MQ=255)
                                                                               gaagaTACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTc                                                               <  1:1488242/149‑1 (MQ=255)
                                                                                aagaTACAGCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACGCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCa                                                              <  2:316700/149‑1 (MQ=255)
                                                                                 agaTACATCAGACAGCGATTCAGGCTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTGGGACAGCGATTCAGACTCAg                                                             <  2:836438/149‑1 (MQ=255)
                                                                                 agaTACATCAGACAGCGATTCAGCCTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACATTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAg                                                             <  2:1608376/149‑1 (MQ=255)
                                                                                 agaTAAACCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAg                                                             <  2:1514401/149‑1 (MQ=255)
                                                                                  gaTACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGa                                                            <  2:1163529/149‑1 (MQ=255)
                                                                                     aCATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAg                                                         >  1:1638303/1‑149 (MQ=255)
                                                                                       aTCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCg                                                       >  1:63936/1‑149 (MQ=255)
                                                                                       aTCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCg                                                       >  2:1538022/1‑149 (MQ=255)
                                                                                         cAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGAGTCGGACAGCTAACCAGACTCAGATAGCGAc                                                     >  1:309842/1‑149 (MQ=18)
                                                                                               gCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtcagat                                               >  1:799509/1‑149 (MQ=255)
                                                                                               gCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtcagat                                               >  1:1020413/1‑149 (MQ=255)
                                                                                               gCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtcagat                                               >  1:1176704/1‑149 (MQ=255)
                                                                                                cGATTCAGACTCAGATAGTGACTCAGGCAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGGTTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGAGTCAGATAGCGACtcagatt                                              >  1:426235/1‑149 (MQ=255)
                                                                                                cGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtcagatt                                              <  1:457623/149‑1 (MQ=255)
                                                                                                  actaaGACTCAGATAGTGACTCAGAAAGCGACTCAGACACAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtcagattca                                            <  2:1208892/145‑1 (MQ=255)
                                                                                                  aTTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtcagattca                                            <  1:438293/149‑1 (MQ=255)
                                                                                                      aGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATa                                        >  1:1546025/1‑149 (MQ=255)
                                                                                                        aCTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGAATCAGGTAGCGACTCAGATTCGGACAGCGACGCAGGCTCAGATAGCGACTCAGAGTCAGATaaa                                      >  2:1715392/1‑147 (MQ=17)
                                                                                                         cTCAGATAGTGACTCAGACAGCGACTCAGCCTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCg                                     <  1:24376/149‑1 (MQ=255)
                                                                                                         cTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACCCAGACTGGGACAGCTATTCAGACTCAGATAGCGAGTCAGATTCAGATAGCg                                     >  2:1462018/1‑149 (MQ=17)
                                                                                                              aTAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATtcg                                >  1:602353/1‑148 (MQ=255)
                                                                                                                  tGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTAAGATAGCGATtcagact                            >  1:1190683/1‑149 (MQ=255)
                                                                                                                  tGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGAATCAGAAAGCGAAtcagaat                            >  2:1581346/1‑147 (MQ=255)
                                                                                                                  tGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCCACTCAGATTCAGATAGCGACTCAGAGTCGGACAGCGATTCAGACTCACATAGCGACTCAGCGTCAGATAGCGAAtcagact                            >  2:961128/1‑149 (MQ=17)
                                                                                                                  tGACTCAGACAGCGAATCAGACTCAGACGGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATtcagact                            >  1:1079812/1‑149 (MQ=255)
                                                                                                                    actcagacagcgactcagactcagacagagactcagACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGAGTCAGATAGCGACTCAGATTCAGATAGAGATacagagtca                          >  2:345814/1‑149 (MQ=17)
                                                                                                                      tcagacagcgactcagactcagacagagactcagccTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGCTCGCGACCCAGATTCGGACAGCGATTCAGACTCCGATGCCGACACAGAATCAGACGGCGATgcagactcagg                        >  2:49495/1‑148 (MQ=14)
                                                                                                                      tcagacagcgactcagactcagacagagactcagacTCAGACAGTGATTCAGATTCAGACAGCGACTCATATTCAGATAGCGACGCAGAAGCGGACAGCGAAGCAGACTCAGATAGCCACTCAGATGCAGACAGCGAcacagacacaga                        >  1:357367/1‑149 (MQ=14)
                                                                                                                      tcagacagcgactcagactcagacagagactcagacTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGCTTCAGACTCCGATAGCGACTCAGATTCAGATAGCGATtcagactccga                        >  1:484694/1‑149 (MQ=255)
                                                                                                                      tcagacagcgactcagactcagacagagactcagacTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGAGTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATtcagactcaga                        >  1:376972/1‑149 (MQ=255)
                                                                                                                       cagacagcgactcagactcagacagagactcagactCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGAAtcagactcagac                       >  1:508188/1‑149 (MQ=255)
                                                                                                                        agacagcgactcagactcagacagcgactcagactcAGACAGTGATTCAGATTCCGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACa                      >  1:855209/1‑149 (MQ=255)
                                                                                                                        agacagcgactcagactcagacagcgactcagactcAGACAGTGATTCAGATTCAGACAGCgactcagactca                                                                                                  <  2:546241/73‑1 (MQ=19)
                                                                                                                        agacagcgactcagactcagacagagactcagactcAGACAGTGATTCAGATTCAGACAGCGACtcagattca                                                                                                  >  1:546241/1‑73 (MQ=255)
                                                                                                                        agacagcgactcagactcagacagagactcagactcAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCCGACTCAGATAGCGACTCAGATTCGGATAGCGCTTCGGACTCAGACa                      >  1:1370252/1‑149 (MQ=14)
                                                                                                                           cagcgactcagactcagacagagactcagactcagaCAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCAGATAGCGACTCGGACCCAGATAGCGATTCAGACCCAGACAGCg                   >  2:1524910/1‑149 (MQ=14)
                                                                                                                            cgcgactcagactcagacagagacccagactcagacaGCGATTCAGATTCAGACATCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGAGTCAGACAGCGa                  <  2:433445/148‑1 (MQ=14)
                                                                                                                               gactcagactcagacagagactcagactcagacagTGATTCAGATTCAGACAg                                                                                                               >  2:1146165/1‑53 (MQ=255)
                                                                                                                               gactcagactcagacagagactcagactcagacagTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACtc               >  1:1380506/1‑149 (MQ=255)
                                                                                                                                       ctcagacagagactcagactcagacagTGATTCAGATTCAGACAGCGACTCAGATTCAGATAACGACTCAGATTCGGACCGCGATTCAGACTCAGATAGCGACTGAGAATCAGAAAGCGATTCACACACAGCCGGCGACtctgatacgg       >  2:1051003/1‑145 (MQ=14)
                                                                                                                                          agacagagactcggactcagacagTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACtcagatgcaggtg    >  1:25570/1‑146 (MQ=255)
                                                                                                                                            acagagacttagactcagacagTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGc  <  2:1023939/149‑1 (MQ=255)
                                                                                                                                            acagagactcagactcagacagTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGc  <  1:1003408/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
TAAAGATGCAGATGGTGGCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGC  >  USA300TCH1516_ALE/622170‑622458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: