| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 740,328:1 | +G | 10.5% | intergenic (+555/+33) | pitA_1 → / ← sle1_2 | Low‑affinity inorganic phosphate transporter 1/N‑acetylmuramoyl‑L‑alanine amidase sle1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 740,328 | 1 | . | G | 10.5% | 971.6 / 116.7 | 247 | intergenic (+555/+33) | pitA_1/sle1_2 | Low‑affinity inorganic phosphate transporter 1/N‑acetylmuramoyl‑L‑alanine amidase sle1 |
| Reads supporting (aligned to +/- strand): ref base . (102/119); new base G (12/14); total (114/133) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.36e-01 | |||||||||||
Only 100 of 260 total aligned reads displayed.
AATATGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAATTGTACGGTAGTTCATA > USA300TCH1516_ALE/740237‑740418 | atatGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGa > 1:1760224/1‑91 (MQ=255) tatgtatgATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGa‑a < 2:418244/91‑1 (MQ=255) tatgtatgATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGa‑a > 2:1703154/1‑91 (MQ=255) atgtatgATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑At < 1:1296216/91‑1 (MQ=255) atgtatgATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑At < 1:2687324/91‑1 (MQ=255) tatgATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGc > 1:3136304/1‑91 (MQ=255) tatgATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGc > 2:2472698/1‑91 (MQ=255) atgATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCt > 2:2074575/1‑91 (MQ=255) atgATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCt < 2:502416/91‑1 (MQ=255) atgATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCt < 1:1310746/91‑1 (MQ=255) atgATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCt < 2:219560/91‑1 (MQ=255) atgATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCt < 1:2561224/91‑1 (MQ=255) tgATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTa > 2:1902410/1‑91 (MQ=255) tgATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAAT‑TCACATCATGTGAGATTGCTa < 1:2702856/91‑1 (MQ=37) aTAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAAT‑TCACATCATGTGAGATTGCTAGt < 1:654878/91‑1 (MQ=37) aTAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAAT‑TCACATCATGTGAGATTGCTAGt < 2:1197998/91‑1 (MQ=37) aTACGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGt < 1:3056890/91‑1 (MQ=255) tAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGta > 1:2738335/1‑91 (MQ=255) aGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGtat < 1:2615841/91‑1 (MQ=255) aGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAAT‑TCACATCATGTGAGATTGCTAGttt < 1:2004877/91‑3 (MQ=25) aGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAAT‑TCACATCATGTGAGATTGCTAGttt < 1:113680/91‑3 (MQ=25) gggAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGtata > 1:2047377/1‑91 (MQ=255) gggAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAAT‑TCACATCATGTGAGATTGCTAGtttt < 1:3513597/91‑4 (MQ=25) ggAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGtatat > 1:3575911/1‑91 (MQ=255) ggAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAAT‑TCACATCATGTGAGATTGCTAGttttt < 2:2461802/91‑5 (MQ=25) aGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATc < 2:1395245/91‑1 (MQ=255) ggTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAg > 2:3429115/1‑91 (MQ=255) ggTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAg > 1:1851423/1‑91 (MQ=255) gTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGt < 1:10966/91‑1 (MQ=255) tGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTa < 2:1333119/91‑1 (MQ=255) agaTAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACa > 2:2640949/1‑91 (MQ=255) agaTAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACa > 1:1415631/1‑91 (MQ=255) aTAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAat < 1:2781794/91‑1 (MQ=255) aTTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAattta < 2:1168535/91‑1 (MQ=255) aTTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAattta > 1:569514/1‑91 (MQ=255) ttttttGTACCAAAATGAAAATTTTTTTTAAAAATTAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAatttat > 2:1768674/1‑91 (MQ=255) ttttttGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAatttat < 2:3770660/91‑1 (MQ=255) ttttttGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAatttat < 2:3841715/91‑1 (MQ=255) ttttttGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAatttat < 2:569514/91‑1 (MQ=255) tttttGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAatttatt < 1:2667789/91‑1 (MQ=255) tttttGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAatttatt > 2:1241380/1‑91 (MQ=255) ttttGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAatttattt > 1:3564662/1‑91 (MQ=255) tttGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAAtttattta > 2:2300579/1‑91 (MQ=255) ttcgTACCAAAATGAAAATTTTTTTCAAAAACAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAAtttattta > 2:2961085/4‑91 (MQ=255) ttGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATttaa > 2:3190031/1‑91 (MQ=255) ttGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTACttaa > 2:1643488/1‑91 (MQ=255) ttGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAACCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATttaa < 2:3487617/91‑1 (MQ=255) tGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATttaat > 2:2832030/1‑91 (MQ=255) tGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATttaat > 2:872105/1‑91 (MQ=255) tGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATttaat > 1:374143/1‑91 (MQ=255) tGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATttaat < 1:3636832/91‑1 (MQ=255) tGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTACttaat > 2:3145950/1‑91 (MQ=255) ccAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTtaattaat < 2:75601/91‑1 (MQ=255) aaaaTGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATgg < 1:1284652/91‑1 (MQ=255) gAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGaa < 2:367244/91‑1 (MQ=255) gAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGaa < 1:76967/91‑1 (MQ=255) aaaTTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATg < 2:2929924/91‑1 (MQ=255) tttttttCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGtat < 2:3618057/53‑1 (MQ=255) tttttttCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCAt < 2:3217178/91‑1 (MQ=255) ttttCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGc < 1:1121010/91‑1 (MQ=255) ttttCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGc < 1:2961047/91‑1 (MQ=255) tCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAg > 1:2107543/1‑91 (MQ=255) cAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGa > 1:2327107/1‑91 (MQ=255) ttaaaTAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGaa > 2:1552297/3‑91 (MQ=255) aaaaaTAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGaa < 1:3254142/91‑1 (MQ=255) aaaaaTAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATACCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGaa > 2:1726051/1‑91 (MQ=255) aaaaTAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGaaa < 1:2900414/91‑1 (MQ=255) aaaTAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAAc > 1:2489681/1‑91 (MQ=255) aaaaaaCTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCt > 1:438622/1‑91 (MQ=255) aaaaaCTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTg < 1:806979/91‑1 (MQ=255) aaaaaCTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTg > 1:1642269/1‑91 (MQ=255) aaaaaCTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTg < 1:2568846/91‑1 (MQ=255) aaaaaCTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTg > 2:2542461/1‑91 (MQ=255) tataCTAGCAAT‑TCACATCATGTGAGATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGa > 1:3481874/4‑91 (MQ=25) aaaCTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGaa < 2:3828094/91‑1 (MQ=255) aaCTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAg > 2:78063/1‑91 (MQ=255) aaCTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAg > 2:215381/1‑91 (MQ=255) aCTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGc < 2:686093/91‑1 (MQ=255) aCTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGc < 2:383311/91‑1 (MQ=255) aCTAGCAAT‑TCACATCATGTGAGATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCGTAGCTAGAAACTTCTGAAGc > 1:1217072/1‑91 (MQ=25) aCTAGCAAT‑TCACATCATGTGAGATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGc > 2:3883249/1‑91 (MQ=37) cTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCt < 1:92859/91‑1 (MQ=255) tAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTg < 1:2267200/91‑1 (MQ=255) gCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGa < 2:1075321/91‑1 (MQ=255) cAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGaa < 2:3077907/91‑1 (MQ=255) cAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGaa > 2:2264495/1‑91 (MQ=255) aaTCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAAt > 1:3862193/1‑91 (MQ=255) ctcACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAATTGt < 2:3276194/91‑1 (MQ=255) tcACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAATTGTa < 1:751275/91‑1 (MQ=255) cacaTGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAATTGTAc < 2:1745078/91‑1 (MQ=255) tgatgTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAATTGTACGGTa > 2:1798359/1‑91 (MQ=255) atgTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAATTGTACGGTAGt < 2:2918287/91‑1 (MQ=255) tgtgA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAATTGTACGGTAGtt < 1:1703154/91‑1 (MQ=255) tgtgA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAATTGTACGGTAGtt < 1:2179020/91‑1 (MQ=255) gtgA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAATTGTACGGTAGTTc < 1:974005/91‑1 (MQ=255) tgA‑ATTGCTAGTATATATCAGTGCAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAATTGTACGGTAGTTCa < 2:3132795/91‑1 (MQ=255) tgA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAATTGTACGGTAGTTCa > 1:2023270/1‑91 (MQ=255) gA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAATTGTACGGTAGTTCat < 1:1003414/91‑1 (MQ=255) gA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAATTGTACGGTAGTTCat < 1:1447766/91‑1 (MQ=255) a‑aTTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAATTGTACGGTAGTTCata < 1:99505/91‑1 (MQ=255) | AATATGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAATTGTACGGTAGTTCATA > USA300TCH1516_ALE/740237‑740418 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |