| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 77,971:1 | +C | 7.1% | intergenic (+80/‑347) | USA300HOU_RS00350 → / → USA300HOU_RS00355 | Monoacylglycerol lipase/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 77,971 | 1 | . | C | 7.1% | 1030.8 / 71.4 | 238 | intergenic (+80/‑347) | USA300HOU_RS00350/USA300HOU_RS00355 | Monoacylglycerol lipase/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (116/105); new base C (5/12); total (121/117) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.03e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.13e-01 | |||||||||||
Only 100 of 244 total aligned reads displayed.
CTTATAACTAAAACAGTATAGTTCCGTGTATTTGATTATAAGAAATTATGAGGATATTAAACATACTAAGATTAGCTATGAAGAAATCTAT‑GACGATAGATTTTTTCATAGCTATTTTTTATAGTTATAGAGAGGAGTAGACTGTCCAGACTCTTGGATTTTAAATCCGTAAAAAAAACAA > USA300TCH1516_ALE/77881‑78061 | cTTATAACTAAAACAGTATAGTTCCGTGTATTTGATTATAAGAAATTATGAGGATATTAAACATACTAAGATTAGCTATGAAGAAATCTAt < 1:1660616/91‑1 (MQ=255) ttATAACTAAAACAGTATAGTTCCGTGTATTTGATTATAAGAAATTATGAGGATATTAAACATACTAAGATTAGCTATGAAGAAATCTAT‑g > 2:580569/1‑91 (MQ=255) taACTAAAACAGTATAGTTCCGTGTATTTGATTATAAGAAATTCTGAGGATATTAAACATACTAAGATTAGCTATGAAGAAATCTAT‑GACg < 1:3869715/91‑1 (MQ=255) aaCTAAAACAGTATAGTTCCGTGTATTTGATTATAAGAAATTATGAGGATATTAAACATACTAAGATTAGCTATGAAGAAATCTAT‑GACGa < 2:1483364/91‑1 (MQ=255) aaCTAAAACAGTATAGTTCCGTGTATTTGATTATAAGAAATTATGAGGATATTAAACATACTAAGATTAGCTATGAAGAAATCTAT‑GACGa < 2:2938833/91‑1 (MQ=255) aCTAAAACAGTATAGTTCCGTGTATTTTATTATAAGAAATTATGAGGATATTAAACATACTAAGATTAGCTATGAAGAAATCTAT‑GACGAt < 2:421185/91‑1 (MQ=255) aCTAAAACAGTATAGTTCCGTGTATTTGATTATAAGAAATTATGAGGATATTAAACATACTAAGATTAGCTATGAAGAAATCTAT‑GACGAt < 2:251091/91‑1 (MQ=255) cTAAAACAGTATAGTTCCGTGTATTTGATTATAAGAAATTATGAGGATATTAAACATACTAAGATTAGCTATGAAGAAATCTAT‑GACGATa > 1:731158/1‑91 (MQ=255) tAAAACAGTATAGTTCCGTGTATTTGATTATAAGAAATTATGAGGATATTAAACATACTAAGATTAGCTATGAAGAAATCTAT‑GACGATAg < 2:1584933/91‑1 (MQ=255) tAAAACAGTATAGTTCCGTGTATTTGATTATAAGAAATTATGAGGATATTAAACATACTAAGATTAGCTATGAAGAAATCTAT‑GACGATAg < 1:856913/91‑1 (MQ=255) tAAAACAGTATAGTTCCGTGTATTTGATTATAAGAAATTATGAGGATATTAAACATACTAAGATTAGCTATGAAGAAATCTAT‑GACGATAg > 1:541582/1‑91 (MQ=255) aaaaCAGTATAGTTCCGTGTATTTGATTATAAGAAATTATGAGGATATTAAACATACTTAGATTAGCTATGAAGAAATCTAT‑GACGATAGa > 1:3297932/1‑91 (MQ=255) aaaCAGTATAGTTCCGTGTATTTGATTATAAGAAATTATGAGGATATTAAACATACTAAGATTAGCTATGAAGAAATCTAT‑GACGATAGAt > 2:668411/1‑91 (MQ=255) aaaCAGTATAGTTCCGTGTATTTGATTATAAGAAATTATGAGGATATTAAACATACTAAGATTAGCTATGAAGAAATCTAT‑GACGATAGAt < 1:76142/91‑1 (MQ=255) aaCAGTATAGTTCCGTGTATTTGATTATAAGAAATTATGAGGATATTAAACATACTAAGATTAGCTATGAAGAAATCTAT‑GACGATAGAtt > 1:596746/1‑91 (MQ=255) aCAGTATAGTTCCGTGTATTTGATTATAAGAAATTATGAGGATATTAAACATACTAAGATTAGCTATGAAGAAATCTAT‑GACGATAGAttt > 1:1272250/1‑91 (MQ=255) cAGTATAGTTCCGTGTATTTGATTATAAGAAATTATGAGGATATTAAACATACTAAGATTAGCTATGAAGAAATCTAT‑GACGATAGAtttt > 1:2108949/1‑91 (MQ=255) gTATAGTTCCGTGTATTTGATTATAAGAAATTATGAGGATATTAAACATACTAAGATTAGCTATGAAGAAATCTAT‑GACGATAGAtttttt > 1:2300456/1‑91 (MQ=255) tataGTTCCGTGTATTTGATTATAAGAAATTATGAGGATATTAAACATACTAAGATTAGCTATGAAGAAATCTAT‑GACGATAGATTTTTTc < 2:3180001/91‑1 (MQ=255) ataGTTCCGTGTATTTGATTATAAGAAATTATGAGGATATTAAACATACTAAGATTAGCTATGAAGAAATCTAT‑GACGATAGAtttttttc > 2:2156943/1‑89 (MQ=255) ataGTTCCGTGTATTTGATTATAAGAAATTATGAGGATATTAAACATACTAAGATTAGCTATGAAGAAATCTAT‑GACGATAGATTTTTTCa > 2:3787068/1‑91 (MQ=255) ataGTTCCGTGTATTTGATTATAAGAAATTATGAGGATATTAAACATACTAAGATTAGCTATGAAGAAATCTAT‑GACGATAGATTTTTTCa > 2:32792/1‑91 (MQ=255) aGTTCCGTGTATTTGATTATAAGAAATTATGAGGATATTAAACATACTAAGATTAGCTATGAAAAAATCTATCGTC‑ATAGATTTCTTCATa < 1:3036191/91‑1 (MQ=21) gTTCCGTGTATTTGATTATAAGAAATTATGAGGATATTAAACATACTAAGATTAGCTATGAAAAAATCTATCGTC‑ATAGATTTCTTCATAg < 1:1991842/91‑1 (MQ=21) gTTCCGTGTATTTGATTATAAGAAATTATGAGGATATTAAACATACTAAGATTAGCTATGAAAAAATCTATCGTC‑ATAGATTTCTTCATAg < 1:3378065/91‑1 (MQ=21) ttCCGTGTATTTGATTATAAGAAATTATGAGGATATTAAACATACTAAGATTAGCTATGAAGAAATCTAT‑GACGATAGATTTTTTCATAGc < 1:1752749/91‑1 (MQ=255) tCCGTGTATTTGATTATAAGAAATTATGAGGATATTAAACATACTAAGATTAGCTATGAAGAAATCTAT‑GACGATAGATTTTTTCATAGCt > 1:3312864/1‑91 (MQ=255) tCCGTGTATTTGATTATAAGAAATTATGAGGATATTAAACATACTAAGATTAGCTATGAAGAAATCTAT‑GACGATAGATTTTTTCATAGCt > 2:1372561/1‑91 (MQ=255) tCCGTGTATTTGATTATAAGAAATTATGAGGATATTAAACATACTAAGATTAGCTATGAAGAAATCTAT‑GACGATAGATTTTTTCATAGCt > 1:3439076/1‑91 (MQ=255) tCCGTGTATTTGATTATAAGAAATTATGAGGATATTAAACATACTAAGATTAGCTATGAAAAAATCTATCGTC‑ATAGATTTCTTCATAGCt < 1:2292167/91‑1 (MQ=21) cGTGTATTTGATTATAAGACATTATGAGGATATTAAAGATACTAAGATTAGCTATGAAGAAATCTAT‑GACGATAGATTTTTTCATAGCTAt > 2:3520393/1‑91 (MQ=255) cGTGTATTTGATTATAAGAAATTATGAGGATATTAAACATACTAAGATTAGCTATGAAGAAATCTAT‑GACGATAGATTTTTTCATAGCTAt < 2:122189/91‑1 (MQ=255) cGTGTATTTGATTATAAGAAATTATGAGGATATTAAACATACTAAGATTAGCTATGAAGAAATCTAT‑GACGATAGATTTTTTCATAGCTAt > 2:3476304/1‑91 (MQ=255) gtgtATTTGATTATAAGAAATTATGAGGATATTAAACATACTAAGATTAGCTATGAAGAAATCTAT‑GACGATAGATTTTTTCATAg < 2:439466/86‑1 (MQ=255) gtgtATTTGATTATAAGAAATTATGAGGATATTAAACATACTAAGATTAGCTATGAAGAAATCTAT‑GACGATAGATTTTTTCATAGCTAtt > 1:238495/1‑91 (MQ=255) gtgtATTTGATTATAAGAAATTATGAGGATATTAAACATACTAAGATTAGCTATGAAAAAATCTATCGTC‑ATAGATTTCTTCATAGCTaat < 2:2819344/91‑3 (MQ=18) tgtATTTGATTATAAGAAATTATGAGGATATTAAACATACTAAGATTAGCTATGAAGAAATCTAT‑GACGATAGATTTTTTCATAGCTAttt < 1:3657874/91‑1 (MQ=255) aTTTGATTATAAGAAATTATGAGGATATTAAACATACTAAGATTAGCTATGAAGAAATCTAT‑GACGATAGATTTTTTCATAGCTAtttttt > 1:1183007/1‑91 (MQ=255) tttGATTATAAGAAATTATGAGGATATTAAACATACTAAGATTAGCTATGAAGAAATCTAT‑GACGATAGATTTTTTCATAGCTATTTTtta > 2:3588362/1‑91 (MQ=255) tGATTATAAGAAATTATGAGGATATTAAACATACTAAGATTAGCTATGAAGAAATCTAT‑GACGATAGATTTTTTCATAGCTATTTTttatt > 2:426604/1‑90 (MQ=255) aTTATAAGAAATTATGAGGATATTAAACATACTAAGATTAGCTATGAAGAAATCTAT‑GACGATAGATTTTTTCATAGCTATTTTttatagt < 1:3757625/91‑1 (MQ=255) aTTATAAGAAATTATGAGGATATTAAACATACTAAGATTAGCTATGAAGAAATCTAT‑GACGATAGATTTTTTCATAGCTATTTTttatagt < 2:3435126/91‑1 (MQ=255) tataAGAAATTATGAGGATATTAAACATACTAAGATTAGCTATGAAGAAGTCTAT‑GACGATAGATTTTTTAATAGCTATTTTttatagtta < 2:101412/91‑1 (MQ=255) ataAGAAATTATGAGGATATTAAACATACTAAGATTAGCTATGAAGAAATCTAT‑GACGATAGATTTTTTCATAGCTATTTTttatagttat < 2:1944184/91‑1 (MQ=255) taAGAAATTATGAGGATATTAAACATACTAAGATTAGCTATGAAGAAATCTAT‑GACGATAGATTTTTTCAt > 2:1190687/1‑71 (MQ=255) taAGAAATTATGAGGATATTAAACATACTAAGATTAGCTATGAAGAAATCTAT‑GACGATAGATTTTTTCATAGCTATTTTttatagttata > 1:1753012/1‑91 (MQ=255) taAGAAATTATGAGGATATTAAACATACTAAGATTAGCTATGAAGAAATCTAT‑GACGATAGATTTTTTCATAGCTATTTTttatagttata < 2:1832985/91‑1 (MQ=255) gAAATTATGAGGATATTAAACATACTAAGATTAGCTATGAAGAAATCTAT‑GACGATAGATTTTTTCATAGCTATTTTTTATAGTTATagag < 2:1024883/91‑1 (MQ=255) aaaTTATGAGGATATTAAACATACTAAGATTAGCTATGAAGAAATCTAT‑GACGATAGATTTTTTCATAGCTATTTTTTATAGTTATagaga > 2:1955472/1‑91 (MQ=255) aaTTATGAGGATATTAAACATACTAAGATTAGCTATGAAGAAATCTAT‑GACGATAGATTTTTTCATAGCTATTTTTTATAGTTATagagag < 2:1964577/91‑1 (MQ=255) ttATGAGGATATTAAACATACTAAGATTAGCTATGAAGAAATCTAT‑GTCGGTAGATTTTTTCATAGCTATTTTTTATAGTTATAGAgagga < 2:2518665/91‑1 (MQ=255) ttATGAGGATATTAAACATACTAAGATTAGCTATGAAGAAATCTAT‑GACGATAGATTTTTTCATTGCTATTTTTTATAGTTATAGAgagga < 2:2217044/91‑1 (MQ=255) ttATGAGGATATAAAACATACTAAGATTAGCTATGAAGAAATCTAT‑GACGATAGATTTTTTCATAGCTATTTTTTATAGTTATAGAgaggt > 1:2980298/1‑90 (MQ=255) tATGAGGATATTAAACATACTAAGATTAGCTATGAAGAAATCTAT‑GACGATAGATTTTTTCATAGCTATTTTTTATAGTTATAGAgaggag < 1:2229015/91‑1 (MQ=255) aTGAGGATATTAAACATACTAAGATTAGCTATGAAGAAATCTAT‑GACGATAGATTTTTTCATAGCTATTTTTTATAGTTATAGAGAGGAGt > 2:3320720/1‑91 (MQ=255) gAGGATATTAAACATACTAAGATTAGCTATGAAGAAATCTAT‑GACGATAGATTTTTTCATAGCTATTTTTTATAGTTATAGAGAGGAGTAg > 1:3395310/1‑91 (MQ=255) aGGATATTAAACATACTAAGATTAGCTATGAAGAAATCTAT‑GACGATAGATTTTTTTATAGCTATTTTTTATAGTTATAGAGAGGAGTAGa > 2:2678982/1‑91 (MQ=255) ggATATTAAACATACTAAGATTAGCTATGAAGAAATCTAT‑GACGATAGATTTTTTCATAGCTATTTTTTATAGTTATAGAGAGGAGTAGAc > 2:833680/1‑91 (MQ=255) atTAAACATACTAAGATTAGCTATGAAGAAATCTAT‑GACGATAGATTTTTTCATAGCTATTTTTTATAGTTATAGAGAGGAGTAGACTGTc < 2:751199/91‑1 (MQ=255) ttAAACATACTAAGATTAGCTATGAAGAAATCTAT‑GACGATAGATTTTTTCATAGCTATTTTTTATAGTTATAGAGAGGAGTAGACTGTcc < 1:1830812/91‑1 (MQ=255) cATACTAAGATTAGCTATGAAGAAATCTAT‑GACGATAGATTTTTTCATAGCTATTTTTTATAGTTATAGAGAGGAGTAGACTGTCCAGAct > 2:1856627/1‑91 (MQ=255) cATACTAAGATTAGCTATGAAGAAATCTAT‑GACGATAGATTTTTTCATAGCTATTTTTTATAGTTATAGAGAGGAGTAGACTGTCCAGAct > 1:2607304/1‑91 (MQ=255) tACTAAGATTAGCTATGAAGAAATCTAT‑GACGATAGATTTTTTCATGGCTATTTTTTATAGTTATAGAGAGGAGTAGACTGTCCAGActct < 2:2078207/91‑1 (MQ=255) tACTAAGATTAGCTATGAAGAAATCTAT‑GACGATAGATTTTTTCATAGCTATTTTTTATAGTTATAGAGAGGAGTAGACTGTCCAGActct > 1:2623302/1‑91 (MQ=255) cTAAGATTAGCTATGAAGAAATCTAT‑GACGATAGATTTTTTCATAGCTATTTTTTATAGTTATAGAGAGGAGTAGACTGTCCAGACTCTTg > 2:3045412/1‑91 (MQ=255) tAAGATTAGCTTTGAAGAAATCTAT‑GACGATAGATTTTTTCATAGCTATTTTTTATAGTTATAGAGAGGAGTAGACTGTCCAGACTCTTgg < 1:3519033/91‑1 (MQ=255) tAAGAGTAGCTATGAAGAAATCTAT‑GACGATAGATTTTTTCATAGCTATTTTTTATAGTTATAGAGAGGAGTAGACTGTCCAGACTCTTgg < 2:3569031/91‑1 (MQ=255) aaGATTAGCTATGAAGAAATCTAT‑GACGATAGATTTTTTCATAGCTATTTTTTATAGTTATAGAGAGGAGTAGACTGTCCAGACTCTTGGa < 2:931320/91‑1 (MQ=255) aGATTAGCTATGAAGAAATCTAT‑GACGATAGATTTTTTCATAGCTATTTTTTATAGTTATAGAGAGGAGTAGACTGTCCAGACTCTTGGAt < 2:3543353/91‑1 (MQ=255) aGATTAGCTATGAAGAAATCTAT‑GACGATAGATTTTTTCATAGCTATTTTTTATAGTTATAGAGAGGAGTAGACTGTCCAGACTCTTGGAt < 1:3484368/91‑1 (MQ=255) aGATTAGCTATGAAGAAATCTAT‑GACGATAGATTTTTTCATAGCTATTTTTTATAGTTATAGAGAGGAGTAGACTGTCCAGACTCTTGGAt > 1:1486946/1‑91 (MQ=255) aGATTAGCTATGAAGAAATCTAT‑GACGATAGATTTTTTCATAGCTATTTTTTATAGTTATAGAGAGGAGTAGACTGTCCAGACTCTTGGAt > 1:1112896/1‑91 (MQ=255) gATTAGCTATGAAGAAATCTAT‑GACGATAGATTTTTTCATAGCTATTTTTTATAGTTATAGAGAGGAGTAGACTGTCCAGACTCTTGGAtt < 1:2421542/91‑1 (MQ=255) aatAGCTATGAAAAAATCTATCGTC‑ATAGATTTCTTCATAGCTATTTTTTATAGTTATAGAGAGGAGTAGACTGTCCAGACTCTTGGAttt > 2:2673880/3‑91 (MQ=17) aTTAGCTATGAAGAAATCTAT‑GACGATAGATTTTTTCATAGCTATTTTTTATAGTTATAGAGAGGAGTAGACTGTCCAGACTCTTGGAttt < 1:493453/91‑1 (MQ=255) ttAGCTATGAAGAAATCTAT‑GACGATAGATTTTTTCATAGCTATTTTTTATAGTTATAGAGAGGAGTAGACTGTCCAGACTCTTGGAtttt < 2:2300456/91‑1 (MQ=255) cTATGAAGAAATCTAT‑GACGATAGATTTTTTCATAGCTATTTTTTATAGTTATAGAGAGGAGTAGACTGTCCAGACTCTTGGATTTTAAAt > 1:2470617/1‑91 (MQ=255) cTATGAAGAAATCTAT‑GACGATAGATTTTTTCATAGCTATTTTTTATAGTTATAGAGAGGAGTAGACTGTCCAGACTCTTGGATTTGAAAt > 1:2512827/1‑91 (MQ=255) tATGAAGAAATCTAT‑GACGATAGATTTTTTCATAGCTATTTTTTATAGTTATAGAGAGGAGTAGACTGTCCAGACTCTTGGATTTTAAATc < 1:1072397/91‑1 (MQ=255) aTGAAGAAATCTAT‑GACGATAGATTTTTTCATTGCTATTTTTTATAGTTATAGAGAGGAGTAGACTGTCCAGACTCTTGGATTTTAAATcc > 2:2386706/1‑91 (MQ=255) aTGAAGAAATCTAT‑GACGATAGATTTTTTCATAGCTATTTTTTATAGTTATAGAGAGGAGTAGACTGTCCAGACTCTTGGATTTTAAATcc > 2:907138/1‑91 (MQ=255) aTGAAGAAATCTAT‑GACGATAGATTTTTTCATAGCTATTTTTTATAGTTATAGAGAGGAGTAGACTGTCCAGACTCTTGGATTTTAAATcc > 2:3697193/1‑91 (MQ=255) tGAAGAAATCTAT‑GACGATAGATTTTTTCATAGCTATTTTTTATAGTTATAGAGAGGAGTAGACTGTCCAGACTCTTGGATTTTAAATCCg < 1:838269/91‑1 (MQ=255) tGAAGAAATCTAT‑GACGATAGATTTTTTCATAGCTATTTTTTATAGTTATAGAGAGGAGTAGACTGTCCAGACTCTTGGATTTTAAATCCg < 1:838507/91‑1 (MQ=255) gaagaaATCTAT‑GACGATAGATTTTTTCATAGCTATTTTTTATAGTTATAGAGAGGAGTAGACTGTCCAGACTCTTGGATTTTAAATCCGt > 1:100186/1‑91 (MQ=255) gaagaaATCTAT‑GACGATAGATTTTTTCATAGCTATTTTTTATAGTTATAGAGAGGAGTAGACTGTCCAGACTCTTGGATTTTAAATCCGt > 1:95056/1‑91 (MQ=255) aagaaATCTAT‑GACGATAGATTTTTTCATAGCTATTTTTTATAGTTATAGAGAGGAGTAGACTGTCCAGACTCTTGGATTTTAAATCCGTa > 1:2702281/1‑91 (MQ=255) aagaaATCTAT‑GACGAGAGATTTTTGCATAGCTATTTTTTATAGTTATAGAGAGGAGTAGACTGTCCAGACTCTTGGATTTTAAATCCGTa < 2:1101084/91‑1 (MQ=255) agaaATCTAT‑GACGATAGATTTTTTCATAGCTATTTTTTATAGTTATAGAGAGGAGTAGACTGTCCAGACTCTTGGATTTTAAATCCGTaa < 2:3629105/91‑1 (MQ=255) agaaATCTAT‑GACGATAGATTTTTCCATAGCTATTTTTTATAGCTATAGAAAGGAGTAGACTGTCCAGACTCTTGGATTTTAAATCCGTaa < 2:3611959/91‑1 (MQ=255) agaaATCTAA‑GACGATAGATTTTTTCATAGCTATTTTTTATAGTTATAGAGAGGAGTAGACTGTCCAGACTCTTGGATTTTAAATCCATaa < 2:1346070/91‑1 (MQ=255) gaaATCTAT‑GACGATAGATTTTTTCATAGCTATTTTTTATAGTTATAGAGAGGAGTAGACTGTCCAGACTCTTGGATTTTAAATCCGTaaa < 1:3728070/91‑1 (MQ=255) aaTCTAT‑GACGATAGATTTTTTCATAGCTATTTTTTATAGTTATAGAGAGGAGTAGACTGTCCAGACTCTTGGATTTTAAATCCGTaaaaa > 2:2690268/1‑91 (MQ=255) aTCTAT‑GACGATAGATTTTTTCATAGCTATTTTTTATTGTTATAGAGAGGAGTAGACTGTCCAGACTCTTGGATTTTAAATCCGTaaaaaa > 2:2739711/1‑91 (MQ=255) tCTAT‑GACGATAGATTTTTTCATAGCTATTTTTTATAGTTATAGAGAGGAGTAGACTGTCCAGACTCTTGGATTTTAAATCCGTaaaaaaa > 1:3485394/1‑91 (MQ=255) tAT‑GACGATAGATTTTTTCATAGCTATTTTTTATAGTTATAGAGAGGAGTAGACTGTCCAGACTCTTGGATTTTAAATCCGTaaaaaaaaa < 1:2859455/91‑2 (MQ=255) aT‑GACGATAGATTTTTTCATAGCTATTTTTTATAGTTATAGAGAGGAGTAGACTGTCCAGAct < 2:3857081/63‑1 (MQ=255) aT‑GACGATAGATTTTTTCATAGCTATTTTTTATAGTTATAGAGAGGAGTAGACTGTCCAGAct > 1:3857081/1‑63 (MQ=255) aT‑GACGATAGATTTTTTCATAGCTATTTTTTATAGTTATAGAGAGGAGTAGACTGTCCAGACTCTTGGATTTTAAATCCGTAAAAAAAACa > 1:3201488/1‑91 (MQ=255) aT‑GACGATAGATTTTTTCATAGCTATTTTTTATAGTTATAGAGAGGAGTAGACTGTCCAGACTCTTGGATTTTAAATCCGTAAAAAAAACa > 2:3050457/1‑91 (MQ=255) | CTTATAACTAAAACAGTATAGTTCCGTGTATTTGATTATAAGAAATTATGAGGATATTAAACATACTAAGATTAGCTATGAAGAAATCTAT‑GACGATAGATTTTTTCATAGCTATTTTTTATAGTTATAGAGAGGAGTAGACTGTCCAGACTCTTGGATTTTAAATCCGTAAAAAAAACAA > USA300TCH1516_ALE/77881‑78061 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |