| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 2,582,146:1 | +A | 10.6% | coding (1174/1353 nt) | pnbA → | Para‑nitrobenzyl esterase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 2,582,146 | 1 | . | A | 10.6% | 686.2 / 80.7 | 189 | coding (1174/1353 nt) | pnbA | Para‑nitrobenzyl esterase |
| Reads supporting (aligned to +/- strand): ref base . (89/80); new base A (18/2); total (107/82) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.39e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.18e-01 | |||||||||||
Only 100 of 215 total aligned reads displayed.
ATACAAGATTCAAACGCACAACTATGGCTTGCTGAATTTGCATGGCATGATACTTCTAGCGCACACTATCGCAGTGCTTACCATATTCTAG‑ATATGGTATTTTGGTTCGGAAACTTACAAATATTAGCAGCACATCAATATCCAACAACTGCTCACCTTAAATTTTTAAGTCGACAAATG > USA300TCH1516_ALE/2582056‑2582235 | ataCAAGATTCAAACGCACAACTATGGCTTGCTGAATTTGCATGGCATGATACTTCTAGCGCACACTATCGCAGTGCTTACCATATTCTAg < 1:372816/91‑1 (MQ=255) taCAAGATTCAAACGCACAACTATGGCTTGCTGAATTTGCATGGCATGATACTTCTAGCGCACACTATCGCAGTGCTTACCATATTCTAG‑a > 1:1305982/1‑91 (MQ=255) taCAAGATTCAAACGCACAACTATGGCTTGCTGAATTTGCATGGCATGATACTTCTAGCGCACACTATCGCAGTGCTTACCATATTCTAG‑a < 2:2310406/91‑1 (MQ=255) taCAAGATTCAAACGCACAACTATGGCTTGCTGAATTTGCATGGCATGATACTTCTAGCGCACACTATCGCAGTGCTTACCATA‑TCTAG‑aa < 2:3107517/91‑2 (MQ=255) taCAAGATTCAAACGCACAACTATGGCTTGCTGAATTTGCATGGCATGATACTTCTAGCGCACACTATCGCAGTGCTTACCATA‑TCTAG‑aa < 2:1644554/91‑2 (MQ=255) gaCAAGATTCAAACGCACAACTATGGCTTGCTGAATGTGCATGGCATGATACTTCTAGCGCACACTATCGCAGTGCTTACCATA‑TCTAG‑aa < 2:3837186/90‑2 (MQ=255) aCAAGATTCAAACGCACAACTATGGCTTACTGAATTTGCATGGCATGATACTTCTAGCGCACACTATCGCAGTGCTTACCATATTCTAG‑at < 1:742524/91‑1 (MQ=255) aCAAGATGCAAACGCACAACCATGGCTTGCTGAAGTTGCATGGCATGATACTTCTAGCGCACACTATCGCAGTGCTTACCATA‑TCTAG‑aat < 2:1713595/91‑3 (MQ=255) cAAGATTCAAACGCACAACTATGGCTTGCTGAATTTGCATGGCATGATACTTCTAGCGCACACTATCGCAGTGCTTACCATATTCTAG‑ata > 1:1431233/1‑91 (MQ=255) cAAGATTCAAACGCACAACTATGGCTTGCTGAATTTGCATGGCATGATACTTCTAGCGCACACTATCGCAGTGCTTACCATATTCTAG‑ata > 2:2735306/1‑91 (MQ=255) aaGATTCAAACGCACAACTATGGCTTGCTGAATTTGCATGGCATGATACTTCTAGCGCACACTATCGCAGTGCTTACCATATTCTAG‑atat < 1:759800/91‑1 (MQ=255) aaGATTCAAACGCACAACTATGGCTTGCTGAATTTGCATGGCATGATACTTCTAGCGCACACTATCGCAGTGCTTACCATATTCTAG‑atat > 2:1984935/1‑91 (MQ=255) gATTCAAACGCACAACTATGGCTTGCTGAATTTGCATGGCATGATACTTCTAGCGCACACTATCGCAGTGCTTACCATATTCTAG‑ATATgg > 1:1419767/1‑91 (MQ=255) gATTCAAACGCACAACTATGGCTTGCTGAATTTGCATGGCATGATACTTCTAGCGCACACTATCGCAGTGCTTACCATA‑TCTAGAATATgg < 1:861698/91‑1 (MQ=255) aTTCAAACGCACAACTATGGCTTGCTGAATTTACATGGCATGATACTTCTAGCGCACACTATCGCAGTGCTTACCATATTCTAG‑ATATGGt < 2:2393319/91‑1 (MQ=255) ttCAAACGCACAACTATGGCTTGCTGAATTTGCATGGCATGATACTTCTAGCGCACACTATCGCAGTGCTTACCATATTCTAG‑ATATGGTa < 1:711823/91‑1 (MQ=255) ttCAAACGCACAACTATGGCTTGCTGAATTTGCATGGCATGATACTTCTAGCGCACACTATCGCAGTGCTTACCATATTCTAG‑ATATGGTa > 1:574784/1‑91 (MQ=255) gtCAAACGCACTACTATGGCTTGCTGAATTTGCATGGCATGATACTTCTAGCGCACACTATCGCAGTGCTTACCATA‑TCTAGAATATGGTa < 2:927039/90‑1 (MQ=255) tCAAACGCACAACTATGGCTTGCTGAATTTGCATGGCATGATACTTCTAGCGCACACTATCGCAGTGCTTACCATATTCTAG‑ATATGGTAt < 2:324028/91‑1 (MQ=255) tCAAACGCACAACTATGGCTTGCTGAATTTGCATGGCATGATACTTCTAGCGCACACTATCGCAGTGCTTACCATATTCTAG‑ATATGGTAt < 1:2901238/91‑1 (MQ=255) cAAACGCACAACTATGGCTTGCTGAATTTGCATGGCATGATACTTCTAGCGCACACTATCGCAGTGCTTACCATATTCTAG‑ATATGGTAtt > 1:3537465/1‑91 (MQ=255) cAAACGCACAACTATGGCTTGCTGAATTTGCATGGCATGATACTTCTAGCGCACACTATCGCAGTGCTTACCATATTCTAG‑ATATGGTAtt < 2:1203652/91‑1 (MQ=255) aaaCGCACAACTATGGCTTGCTGAATTTGCATGGCATGATACTTCTAGCGCACACTATCGCAGTGCTTACCATATTCTAG‑ATATGGTAttt > 1:3856949/1‑91 (MQ=255) aaCGCACAACTATGGCTTGCTGAATTTGCATGGCATGATACTTCTAGCGCACACTATCGCAGTGCTTACCATATTCTAG‑ATATGGTAtttt > 1:2544565/1‑91 (MQ=255) aaCGCACAACTATGGCTTGCTGAATTTGCATGGCATGATACTTCTAGCGCACACTATCGCAGTGCTTACCATATTCTAG‑ATATGGTAtttt > 1:3584477/1‑91 (MQ=255) aaCGCACAACTAGGGCTTGCTGAATTTGCATGGCATGATACTTCTAGCGCACACTATCGCAGTGCTTACCATATTCTAG‑ATATGGTAtttt > 1:2976289/1‑91 (MQ=255) aCGCACAACTATGGCTTGCTGAATTTGCATGGCATGATACTTCTAGCGCACACTATCGCAGTGCTTACCATATTCTAG‑ATATGGTATTTTg > 1:1849654/1‑91 (MQ=255) gCACAACTATGGCTTGCTGAATTTGCATGGCATGATACTTCTAGCGCACACTATCGCAGTGCTTACCATATTCTAG‑ATATGGTATTTTGGt < 2:518056/91‑1 (MQ=255) gCACAACTATGGCTTGCTGAATTTGCATGGCATGATACTTCTAGCGCACACTATCGCAGTGCTTACCATATTCTAG‑ATATGGTATTTTGGt < 2:1279749/91‑1 (MQ=255) gCACAACTATGGCTTGCTGAATTTGCATGGCATGATACTTCTAGCGCACACTATCGCAGTGCTTACCATATTCTAG‑ATATGGTATTTTGGt < 1:1349194/91‑1 (MQ=255) cacaACTATGGCTTGCTGAATTTGCATGGCATGATACTTCTAGCGCACACTATCGCAGTGCTTACCATATTCTAG‑ATATGGTATTTTGGtt > 2:3067855/1‑91 (MQ=255) acaACTATGGCTTGCTGAATTTGCATGGCATGATACTTCTAGCGCACACTATCGCAGTGCTTACCATATTCTAG‑ATATGGTATTTTGGTTc > 1:2870225/1‑91 (MQ=255) caACTATGGCTTGCTGAATTTGCATGGCATGATACTTCTAGCGCACACTATCGCAGTGCTTACCATATTCTAG‑ATATGGTATTTTGGTTCg > 1:255679/1‑91 (MQ=255) caACTATGGCTTGCTGAATTTGCATGGCATGATACTTCTAGCGCACACTATCGCAGTGCTTACCATATTCTAG‑ATATGGTATTTTGGTTCg < 1:3500271/91‑1 (MQ=255) caACTATGGCTTGCTGAATTTGCATGGCATGATACTTCTAGCGCACACTATCGCAGTGCTTACCATATTCTAG‑ATATGGTATTTTGGTTCg < 1:3347883/91‑1 (MQ=255) aCTATGGCTTGCTGAATTTGCATGGCATGATACTTCTAGCGCACACTATCGCAGTGCTTACCATATTCTAG‑ATATGGTATTTTGGTTCGGa > 2:1099197/1‑91 (MQ=255) aCTATGGCTTGCTGAATTTGCATGGCATGATACTTCTAGCGCACACTATCGCAGTGCTTACCATATTCTAG‑ATATGGTATTTTGGTTCGGa > 2:3327527/1‑91 (MQ=255) tATGGCTTGCTGAATTTGCATGGCATGATACTTCTAGCGCACACTATCGCAGTGCTTACCATATTCTAG‑ATATGGTATTTTGGTTCGGaaa < 1:1634631/91‑1 (MQ=255) tGGCTTGCTGAATTTGCATGGCATGATACTTCTAGCGCACACTATCGCAGTGCTTACCATATTCTAG‑ATATGGTATTTTGGTTCGGAAACt > 1:24356/1‑91 (MQ=255) gCTTGCTGAATTTGCATGGCATGATACTTCTAGCGCACACTATCGCAGTGCTTACCATATTCTAG‑ATATGGTATTTTGGTTCGGAAACTTa > 1:2034192/1‑91 (MQ=255) gCTTGCTGAATTTGCATGGCATGATACTTCTAGCGCACACTATCGCAGTGCTTACCATATTCTAG‑ATATGGTATTTTGGTTCGGAAACTTa < 1:1207027/91‑1 (MQ=255) gCTTGCTGAATTTGCATGGCATGATACTTCTAGCGCACACTATCGCAGTGCTTACCATATTCTAG‑ATATGGTATTTTGGTTCGGAAACTTa < 2:1738592/91‑1 (MQ=255) cTTGCTGAATTTGCATGGCATGATACTTCTAGCGCACACTATCGCAGTGCTTACCATATTCTAG‑ATATGGTATTTTGGTTCGGAAACTTAc < 2:1451682/91‑1 (MQ=255) ttGCTGAATTTGCATGGCATGATACTTCTAGCGCACACTATCGCAGTGCTTACCATATTCTAG‑ATATGGTATTTTGGTTCGGAAACTTACa < 1:395470/91‑1 (MQ=255) ttGCTGAATTTGCATGGCATGATACTTCTAGCGCACACTATCGCAGTGCTTACCATATTCTAG‑ATATGGTATTTTGGTTCGGAAACTTACa < 1:206943/91‑1 (MQ=255) gCTGAATTTGCATGGCATGATACTTCTAGCGCACACTATCGCAGTGCTTTCCATATTCTAG‑ATATGGTATTTTGGTTCGGAAACTTACaaa < 1:3358162/91‑1 (MQ=255) gCTGAATTTGCATGGCATGATACTTCTAGCGCACACTATCGCAGTGCTTACCATATTCTAG‑ATATGGTATTTTGGTTCGGAAACTTACaaa < 1:3238585/91‑1 (MQ=255) cTGAATTTGCATGGCATGATACTTCTAGCGCACACTATCGCAGTGCTTACCATATTCTAG‑ATATGGTATTTTGGTTCGGAAACTTACAAat < 2:3184802/91‑1 (MQ=255) gAATTTGCATGGCATGATACTTCTAGCGCACACTATCGCAGTGCTTACCATATTCTAG‑ATATGGTATTTTGGTTCGGAAACTTACAAatat < 2:902405/91‑1 (MQ=255) aTTTGCATGGCATGATACTTCTAGCGCACACTATCGCAGTGCTTACCATATTCTAG‑ATATGGTATTTTGGTTCGGAAACTTACAAGTATTa > 2:3588400/1‑91 (MQ=255) aTTTGCATGGCATGATACTTCTAGCGCACACTATCGCAGTGCTTACCATATTCTAG‑ATATGGTATTTTGGTTCGGAAACTTACAAATATTa > 1:1603939/1‑91 (MQ=255) tttGCATGGCATGATACTTCTAGCGCACACTATCGCAGTGCTTACCATATTCTAG‑ATATGGTATTTTGGTTCGGAAACTTACAAATATTag > 2:1836225/1‑91 (MQ=255) gcatggcatgATACTTCTAGCGCACACTATCGCAGTGCTTACCATATTCTAG‑ATATGGTATTTTGGTTCGGAAACTTACAAATATTagcag > 1:1042305/1‑91 (MQ=255) catggcatgATACTTCTAGCGCACACTATCGCCGTGCTTACCATATTCT‑G‑ATATGGTATTTTGGTTCGGAAACTTACAAATATTAgcagca > 2:3804108/1‑91 (MQ=255) catggcatgATACTTCTAGCGCACACTATCGCAGTGCTTACCATATCCTAG‑ATATGGTATTTTgg > 1:3082546/1‑65 (MQ=255) atggcatgATACTTCTAGCGCACACTATCGCAGTGCTTACCATATTCTAG‑ATATGGTATTTTGGTTCGGAAACTTACAAATATTAgcagca > 1:2774816/1‑91 (MQ=255) ggcatgATACTTCTAGCGCACACTATCGCAGTGCTTACCATATTCTAG‑ATATGGTATTTTGGTTCGGAAACTTACAAATATTAGCAGcaca < 1:2741196/91‑1 (MQ=255) gcatgATACTTCTAGCGCACACTATCGCAGTGCTTACCATATTCTAG‑ATATGGTATTTTGGTTCGGAAACTTACAAATATTAGCAGCACAt > 2:585786/1‑91 (MQ=255) atgATACTTCTAGCGCACACTATCGCAGTGCTTACCATATTCTAG‑ATATGGTATTTTGGTTCGGAAACTTACAAATATTAGCAGCACATCa > 1:529513/1‑91 (MQ=255) tgATACTTCTAGCGCACACTATCGCAGTGCTTACCATATTCTAG‑ATATGGTATTTTGGTTCGGAAACTTACAAATATTAGCAGCACATCaa > 2:719206/1‑91 (MQ=255) gATACTTCTAGCGCACACTATCGCAGTGCTTACCATATTCTAG‑ATATGGTATTTTGGTTCGGAAACTTACAAATATTAGCAGCACATCAat > 1:68542/1‑91 (MQ=255) gATACTTCTAGCGCACACTATCGCAGTGCTTACCATATTCTAG‑ATATGGTATTTTGGTTCGGAAACTTACAAATATTAGCAGCACATCAat < 2:2086868/91‑1 (MQ=255) aTACTTCTAGCGCACACTATCGCAGTGCTTACCATATTCTAG‑ATATGGTATTTTGGTTCGGAAACTTACAAATATTAGCAGCACATCAata > 2:3760381/1‑91 (MQ=255) aTACTTCTAGCGCACACTATCGCAGTGCTTACCATATTCTAG‑ATATGGTATTTTGGTTCGGAAACTTACAAATATTAGCAGCACATCAata < 2:1272243/91‑1 (MQ=255) tACTTCTAGCGCACACTATCGCAGTGCTTACCATATTCTAG‑ATATGGTATTTTGGTTCGGAAACTTACAAATATTAGCAGCACATCAatat > 2:102897/1‑91 (MQ=255) tACTTCTAGCGCACACTATCGCAGTGCTTACCATATTCTAG‑ATATGGTATTTTGGTTCGGAAACTTACAAATATTAGCAGCACATCAatat > 1:3071996/1‑91 (MQ=255) caCTTCTAGCGCACACTATCGCAGTGCTTACCATATTCTAG‑ATATGGTATTTTGGTTCGGAAACTTACAAATATTAGCAGCACATCAatat < 2:1766083/90‑1 (MQ=255) cTTCTAGCGCACACTATCGCAGTGCTTACCATATTCTAG‑ATATGGTATTTTGGTTCGGAAACTTACAAATATTAGCAGCACATCAATATcc < 2:664913/91‑1 (MQ=255) ttCTAGCGCACACTATCGCAGTGCTTACCATATTCTAG‑ATATGGTATTTTGGTTCGGAAACTTACAAATATTAGCAGCACATCAATATCca > 1:2842473/1‑91 (MQ=255) ttCTAGCGCACACTATCGCAGTGCTTACCATATTCTAG‑ATATGGTATTTTGGTTCGGAAACTTACAAATATTAGCAGCACATCAATATCca < 1:409961/91‑1 (MQ=255) tAGCGCACACTATCGCAGTGCTTACCATATTCTAG‑ATATGGTATTTTGGTTCGGAAACTTACAAATATTAGCAGCACATCAATATCcaaca < 1:2500812/91‑1 (MQ=255) aGCGCACACTATCGCAGTGCTTACCATATTCTAG‑ATATGGTATTTTGGTTCGGAAACTTACAAATATTAGCAGCACATCAATATCcaacaa > 2:3574795/1‑91 (MQ=255) cgcACACTATCGCAGTGCTTACCATATTCTAG‑ATATGGTATTTTGGTTCGGAAACTTACAAATATTAGCAGCACATCAATATCCAACAACt > 1:1447191/1‑91 (MQ=255) cacaCTATCGCAGTGCTTACCATATTCTAG‑ATATGGTATTTTGGTTCGGAAACTTACAAATATTAGCAGCACATCAATATCCAACAACTGc < 1:192218/91‑1 (MQ=255) acacTATCGCAGTGCTTACCATATTCTAG‑ATATGGTATTTTGGTTCGGAAACTTACAAATATTAGCAGCACATCAATATCCAACAACTGCt < 2:2349497/91‑1 (MQ=255) acacTATCGCAGTGCTTACCATATTCTAG‑ATATGGTATTTTGGTTCGGAAACTTACAAATATTAGCAGCACATCAATATCCAACAACTGCt > 1:1548252/1‑91 (MQ=255) acacTATCGCAGTGCTTACCATATTCTAG‑ATATGGTATTTTGGTTCGGAAACTTACAAATATTAGCAGCACATCAATATCCAACAACTGCt > 1:1970079/1‑91 (MQ=255) cacTATCGCAGTGCTTACCATATTCTAG‑ATATGGTATTTTGGTTCGGAAACTTACAAATATTAGCAGCACATCAATATCCAACAACTGCTc > 1:2535281/1‑91 (MQ=255) cTATCGCAGTGCTTACCATATTCTAG‑ATATGGTATTTTGGTTCGGAAACTTACAAATATTAGCAGCACATCAATATCCAACAACTGCTCAc < 1:981577/91‑1 (MQ=255) tATCGCAGTGCTTACCATATTCTAG‑ATATGGTATTTTGGTTCGGAAACTTACAAATATTAGCAGCACATCAATATCCAACAACTGCTCAcc < 2:2774816/91‑1 (MQ=255) aTCGCAGTGCTTACCATATTCTAG‑ATATGGTATTTTGGTTCGGAAACTTACAAATATTAGCAGCACATCAATATCCAACAACTGCTCACCt < 1:1712705/91‑1 (MQ=255) aTCGCAGTGCTTACCATATTCTAG‑ATATGGTATTTTGGTTCGGAAACTTACAAATATTAGCAGCACATCAATATCCAACAACTGCTCACCt > 2:2876269/1‑91 (MQ=255) tCGCAGTGCTTACCATATTCTAG‑ATATGGTATTTTGGTTCGGACACTTACAAATATTAGCAGCACATCAATATCCAACAACTGCCCAGCtt > 2:875309/1‑91 (MQ=255) gCAGTGCTTACCATATTCTAG‑ATATGGTATTTTGGTTCGGAAACTTACAAATATTAGCAGCACATCAATATCCAACAACTGCTCACCTTaa < 2:2976289/91‑1 (MQ=255) aGTGCTTACCATATTCTAG‑ATATGGTATTTTGGTTCGGAAACTTACAAATATTAGCAGCACATCAATATCCAACAACTGCTCACCTTAAAt < 1:1149196/91‑1 (MQ=255) gCTTACCATATTCTAG‑ATATGGTATTTTGGTTCGGAAACTTACAAATATTAGCAGCACATCAATATCCAACAACTGCTCACCTTAAAtttt < 2:2757598/91‑1 (MQ=255) gCTTACCATATTCTAG‑ATATGGTATTTTGGTTCGGAAACTTACAAATATTAGCAGCACATCAATATCCAACAACTGCTCACCTTAAAtttt > 1:2363746/1‑91 (MQ=255) atACCATA‑TCTAGAATATGGTATTTTGGTTCGGAAACTTACAAATATTAGCAGCACATCAATATCCAACAACTGCTCACCTTAAATTTTTa > 2:938449/2‑91 (MQ=255) tACCATATTCTAG‑ATATGGTATTTTGGTTCGGAAACTTACAAATATTAGCAGCACATCAATATCCAACAACTGCTCACCTTAAATTTTTaa < 1:3282223/91‑1 (MQ=255) tACCATA‑TCTAGAATATGGTATTTTGGTTCGGAAACTTACAAATATTAGAAGCAGATCAATATCCAACAACTGCTCACCTTAAATTTTTat > 2:3865110/1‑90 (MQ=255) ccATA‑TCTAGAATATGGTATTTTGGTTCGGAAACTTACAAATATTAGCAGCACATCAATATCCAACAACTGCTCACCTTAAATTTTTAAGt > 1:1909958/1‑91 (MQ=255) catatCTAGAATATGGTATTTTGGTTCGGAAACTTACAAATATTAGCAGCACATCAATATCCAACAACTGCTCACCTTAAATTTTTAAGTc > 1:1724879/5‑91 (MQ=255) atatTCTAG‑ATATGGTATTTTGGTTCGGAAACTTACAAATATTAGCAGCACATCAATATCCAACAACTGCTCACCTTAAATTTTTAAGTCg < 2:2662790/91‑1 (MQ=255) atatTCTAG‑ATATGGTATTTTGGTTCGGAAACTTACAAATATTAGCAGCACATCAATATCCAACAACTGCTCACCTTAAATTTTTAAGTCg > 1:1497628/1‑91 (MQ=255) atatCTAGAATATGGTATTTTGGTTCGGAAACTTACAAATATTAGCAGCACATCAATATCCAACAACTGCTCACCTTAAATTTTTAAGTCg > 2:1079294/4‑91 (MQ=255) atatCTAGAATATGGTATTTTGGTTCGGAAACTTACAAATATTAGCAGCACATCAATATCCAACAACTGCTCACCTTAAATTTTTAAGTCg > 1:3437389/4‑91 (MQ=255) tCTAGAATATGGTATTTTGGTTCGGAAACTTACAAATATTAGCAGCACATCAATATCCAACAACTGCTCACCTTAAATTTTTAAGTCGACa > 1:2304952/1‑91 (MQ=255) tCTAG‑ATATGGTATTTTGGTTCGGAAACTTACAAATATTAGCAGCACATCAATATCCAACAACTGCTCACCTTAAATTTTTAAGTCGACaa > 2:2489948/1‑91 (MQ=255) tAG‑ATATGGTATTTTGGTTCGGAAACTTACAAATATTAGCAGCACATCAATATCCAACAACTGCTCACCTTAAATTTTTAAGTCGACAAAt < 2:566100/91‑1 (MQ=255) tAG‑ATATGGTATTTTGGTTCGGAAACTTACAAATATTAGCAGCACATCAATATCCAACAACTGCTCACCTTAAATTTTTAAGTCGACAAAt > 1:2191262/1‑91 (MQ=255) | ATACAAGATTCAAACGCACAACTATGGCTTGCTGAATTTGCATGGCATGATACTTCTAGCGCACACTATCGCAGTGCTTACCATATTCTAG‑ATATGGTATTTTGGTTCGGAAACTTACAAATATTAGCAGCACATCAATATCCAACAACTGCTCACCTTAAATTTTTAAGTCGACAAATG > USA300TCH1516_ALE/2582056‑2582235 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |