| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 581,901 | G→C | 6.9% | V13L (GTG→CTG) | nusG_2 → | Transcription termination/antitermination protein NusG |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 581,901 | 0 | G | C | 6.9% | 655.2 / 26.6 | 188 | V13L (GTG→CTG) | nusG_2 | Transcription termination/antitermination protein NusG |
| Reads supporting (aligned to +/- strand): ref base G (100/75); new base C (10/3); total (110/78) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01 | |||||||||||
Only 100 of 193 total aligned reads displayed.
GATTTAGGAATTACAGCATTGAAAAATTTATTATTTCGTTAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAATCTATGAATATGACTGAACAAATCTTTAGAG > USA300TCH1516_ALE/581811‑581991 | gATTTAGGAATTACAGCATTGAAAAATTTATTATTTCGTTAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAg > 1:1637842/1‑91 (MQ=255)gATTTAGGAATTACAGCATTGAAAAATTTATTATTTCGTTAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAg > 2:887903/1‑91 (MQ=255) tttAGGAATTACAGCATTGAAAAATTTATTATTTCGTTAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTg > 2:718217/1‑91 (MQ=255) ttAGGAATTACAGCATTGAAAAATTTATTATTTCGTTAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCACTGc < 1:464175/91‑1 (MQ=255) aGGAATTACAGCATTGAAAAATTTATTATTTCGTTAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGcat < 1:137298/91‑1 (MQ=255) aGGAATTACAGCATTGAAAAATTTATTATTTCGTTAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGcat < 1:764663/91‑1 (MQ=255) aGGAATTACAGCATTGAAAAATTTATTATTTCGTTAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGcat < 1:764544/91‑1 (MQ=255) ggAATTACAGCATTGAAAAATTTATTATTTCGTTAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGcata < 1:437669/91‑1 (MQ=255) ggAATTACAGCATTGAAAAATTTATTATTTCGTTAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGcata < 1:2143040/91‑1 (MQ=255) ggAATTACAGCATTGAAAAATTTATTATTTCGTTAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGcata < 1:2141935/91‑1 (MQ=255) ttACAGCATTGAAAAATTTATTATTTCGTTAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGcatacata < 2:3477781/91‑1 (MQ=255) tACAGCATTGAAAAATTTATTATTTCGTTAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACatat > 1:3706410/1‑91 (MQ=255) tACAGCATTGAAAAATTTATTATTTCGTTAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACatat > 2:3567680/1‑91 (MQ=255) cAGCATTGAAAAATTTATTATTTCGTTAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTc > 1:2182562/1‑91 (MQ=255) cAGCATTGAAAAATTTATTATTTCGTTAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTc > 1:3290760/1‑91 (MQ=255) gCATTGAAAAATTTATTATTTCGTTAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTg > 2:160359/1‑91 (MQ=255) gAAAAATTTATTATTTCGTTAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGatat < 2:2301233/91‑1 (MQ=255) aaaaaTTTATTATTTCGTTAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATg < 1:2307758/91‑1 (MQ=255) aaaaTTTATTATTTGGTTAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGa < 1:1401888/91‑1 (MQ=255) aaaaTTTATTATTTCGTTAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGa > 2:3584096/1‑91 (MQ=255) aaaaTTTATTATTTCGTTAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGa > 2:2599755/1‑91 (MQ=255) aaaaTTTATTATTTCGTTAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGa > 2:2043394/1‑91 (MQ=255) aaaaTTTATTATTTCGTTAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGa > 2:2535766/1‑91 (MQ=255) aTTTATTATTTCGTTAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGaaaa > 1:2632479/1‑91 (MQ=255) tttATTATTTCGTTAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAAt > 1:3739175/1‑91 (MQ=255) ttattTCGTTAGAGGAGTGAAGACATGTCTGCAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAg > 1:1895001/1‑91 (MQ=255) ttattTCGTTAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAg > 1:2885506/1‑91 (MQ=255) attTCGTTAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGtt < 2:201716/91‑1 (MQ=255) tCGTTAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTaaa < 1:3036615/91‑1 (MQ=255) tCGTTAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTaaa < 2:3204838/91‑1 (MQ=255) tCGTTAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTaaa < 2:1545629/91‑1 (MQ=255) cGTTAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTaaaa < 1:1166737/91‑1 (MQ=255) ttAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAg < 2:1274757/91‑1 (MQ=255) agagGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGaa < 2:3770273/91‑1 (MQ=255) aggagTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAAtt > 1:1996346/1‑91 (MQ=255) aagagTGAAGACATGTCTGAAGAAGTTGTCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAAtt < 2:1273082/89‑1 (MQ=255) gagTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTa < 1:2963878/91‑1 (MQ=255) gagTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTa < 2:1860668/91‑1 (MQ=255) gTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGa > 2:300063/1‑91 (MQ=255) gTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGa < 1:250668/91‑1 (MQ=255) gAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGaaa > 2:47011/1‑91 (MQ=255) aaGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGaaaa > 1:3762461/1‑91 (MQ=255) aaGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGaaaa < 1:1578023/91‑1 (MQ=255) gACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGaaaaaa < 1:1531805/91‑1 (MQ=255) aCATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAag < 2:2923542/91‑1 (MQ=255) aCATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAag < 1:893892/91‑1 (MQ=255) tGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAGGTTAAAAAAAAAAAAAAAAAAAGAGt < 2:3739175/91‑1 (MQ=255) tGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGt < 2:3290760/91‑1 (MQ=255) tGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGt < 1:2535766/91‑1 (MQ=255) gTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTa < 2:3786160/91‑1 (MQ=255) gTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTa > 1:2803647/1‑91 (MQ=255) gTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTa > 2:2501859/1‑91 (MQ=255) tCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAg > 1:2302669/1‑91 (MQ=255) cTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGa > 1:2173170/1‑91 (MQ=255) cTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGa > 2:760798/1‑91 (MQ=255) tGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGaa < 2:3173049/91‑1 (MQ=255) gaagaaGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAAt < 1:2386512/91‑1 (MQ=255) aagaagTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGt > 2:1156981/1‑85 (MQ=255) aagaagTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAATc > 1:679703/1‑91 (MQ=255) aagaagTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAATc > 1:3012936/1‑91 (MQ=255) gaagTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAATCTa < 2:2729985/91‑1 (MQ=255) aagTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAATCTAt > 2:3315849/1‑91 (MQ=255) agTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAATCTATg > 2:3677245/1‑91 (MQ=255) ttGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAATCTATGaa > 2:1985361/1‑91 (MQ=255) ttGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAAAAAAAAAAAAAAAGAGTAGAATCTATGaa < 2:1220489/91‑1 (MQ=255) ggCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAATCTATGAata > 2:900818/1‑91 (MQ=255) gcgcAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAATCTATGAatat > 1:1141897/1‑91 (MQ=255) gcgcAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAATCTATGAatat > 1:2289457/1‑91 (MQ=255) gcgcAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAATCTATGAatat > 1:3582304/1‑91 (MQ=255) gcAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAATCTATGAATATGa > 2:2350613/1‑91 (MQ=255) aaGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAATCTATGAATATGACTg > 1:274134/1‑91 (MQ=255) aGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAATCTATGAATATGACTGa > 1:1013248/1‑91 (MQ=255) aGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAATCTATGAATATGACTGa < 2:2688381/91‑1 (MQ=255) aGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAATCTATGAATATGACTGa > 2:2712621/1‑91 (MQ=255) aGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAATCTATGAATATGACTGa > 2:577055/1‑91 (MQ=255) aGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAATCTATGAATATGACTGa > 2:1232584/1‑91 (MQ=255) cGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAATCTATGAATATGACTGAAc < 2:2182562/91‑1 (MQ=255) cGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAATCTATGAATATGACTGAAc < 1:3186729/91‑1 (MQ=255) cGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAATCTATGAATATGACTGAAc < 2:1831686/91‑1 (MQ=255) cGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAATCTATGAATATGACTGAAc > 2:1542537/1‑91 (MQ=255) gTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAATCTATGAATATGACTGAACa > 2:3113389/1‑91 (MQ=255) atatgTATGCACTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAATCTATGAATATGACTGAACa > 1:2810152/5‑91 (MQ=255) tGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAATCTATGAATATGACTGAACaaa > 2:79619/1‑91 (MQ=255) tGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAATCTATGAATATGACTGAACaaa > 2:1039950/1‑91 (MQ=255) tGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAATCTATGAATATGACTGAACaaa > 1:3479627/1‑91 (MQ=255) atgTATGCACTGCATACCTATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAATCTATGAATATGACTGAACaaa > 1:171529/3‑91 (MQ=255) tgTATGCACTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAATCTATGAATATGACTGAACAAAt > 2:3448648/2‑91 (MQ=255) ggTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAATCTATGTATATGACTGAACAAAt > 2:1970844/1‑91 (MQ=255) ggTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAATCTATGAATATGACTGAACAAAt > 2:3260306/1‑91 (MQ=255) ggTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAATCTATGAATATGACTGAACAAAt > 2:1122075/1‑91 (MQ=255) tATGCACTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAATCTATGAATATGACTGAACAAATCt > 1:3383052/1‑91 (MQ=255) aTGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAATCTATGAATATGACTg < 2:146157/81‑1 (MQ=255) aTGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAATCTATGAATATGACTGAACAAATCtt > 1:2907697/1‑91 (MQ=255) aTGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAATCTATGAATATGACTGAACAAATCtt > 2:1110161/1‑91 (MQ=255) aTGCACTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTCGAAAAAAGAGTAGAATCTATGAATATGACAGAACAAATCtt > 2:3406997/1‑91 (MQ=255) aTGCACTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAATCTATGAATATGACTGAACAAATCtt > 2:322900/1‑91 (MQ=255) tGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGATAAAAGAGTAGAATCTATGAATATGACTGAACAAATCttt > 2:831807/1‑91 (MQ=255) tGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAATCTATGAATATGACTGAACAAATCttt < 2:51347/91‑1 (MQ=255) gCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAATCTATGAATATGACTGAACAAATCTTTa > 2:1715243/1‑91 (MQ=255) gTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAATCTATGAATATGACTGAACAAATCTTTagag < 1:2046530/91‑1 (MQ=255) | GATTTAGGAATTACAGCATTGAAAAATTTATTATTTCGTTAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAATCTATGAATATGACTGAACAAATCTTTAGAG > USA300TCH1516_ALE/581811‑581991 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |