| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 740,322 | G→C | 12.4% | intergenic (+549/+39) | pitA_1 → / ← sle1_2 | Low‑affinity inorganic phosphate transporter 1/N‑acetylmuramoyl‑L‑alanine amidase sle1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 740,322 | 0 | G | C | 12.4% | 768.6 / 84.8 | 249 | intergenic (+549/+39) | pitA_1/sle1_2 | Low‑affinity inorganic phosphate transporter 1/N‑acetylmuramoyl‑L‑alanine amidase sle1 |
| Reads supporting (aligned to +/- strand): ref base G (107/111); new base C (11/20); total (118/131) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.81e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01 | |||||||||||
Only 100 of 270 total aligned reads displayed.
TATAAATATGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAATTGTACGGTAG > USA300TCH1516_ALE/740233‑740412 | tataaatatGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACatga < 1:48391/91‑1 (MQ=255)tataaatatGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACatga > 2:2021244/1‑91 (MQ=255) ataaatatGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACatgat < 1:153118/91‑1 (MQ=255) ataaatatGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACatgat < 2:350996/91‑1 (MQ=255) ataaataaGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACatgat < 1:2753343/91‑1 (MQ=255) taaatatGTATGATAGGGAAGGGTGAGATAATTTTTTTTATTATAATTAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACatgatg > 2:3870248/1‑91 (MQ=255) taaatatGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAAAAAAAAAAAAAAATAAAAAACTAGCAATCTCACatgatg < 2:921067/91‑1 (MQ=255) aaatatGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGAtgt < 1:1408163/91‑1 (MQ=255) aatatGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAAT‑TCACATCATGTGa < 2:812587/91‑1 (MQ=255) atatGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGa > 1:1760047/1‑91 (MQ=255) atatGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGa < 1:2050990/91‑1 (MQ=255) tatgtatgATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGa‑a < 2:418244/91‑1 (MQ=255) atgtatgATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑At < 1:1297042/91‑1 (MQ=255) atgtatgATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑At < 1:2687409/91‑1 (MQ=255) tgtatgATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAAT‑TCACATCATGTGagatt < 1:2564452/91‑5 (MQ=38) gtatgATAGGGAAGGGTGAGATAAATTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTg < 2:2481152/91‑1 (MQ=255) tatgATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGc > 2:2472698/1‑91 (MQ=255) atgATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCt < 1:1310746/91‑1 (MQ=255) atgATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCt < 2:219560/91‑1 (MQ=255) atgATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCt < 2:502416/91‑1 (MQ=255) tgATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAATAATATAAAACTAGCAATCTCACACGATGTGA‑ATTGCTa > 2:1086614/1‑91 (MQ=255) tgATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAAT‑TCACATCATGTGAGATTGCTa < 1:3531372/91‑1 (MQ=37) gATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAg < 2:560575/91‑1 (MQ=255) gATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAAT‑TCACATCATGTGAGATTGCTAg < 1:3250587/91‑1 (MQ=37) atatGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGt < 1:3057052/91‑1 (MQ=255) aTAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAAT‑TCACATCATGTGAGATTGCTAGt < 1:654878/91‑1 (MQ=37) aGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGtat > 1:402713/1‑91 (MQ=255) aGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAAT‑TCACATCATGTGAGATTGCTAGttt < 1:2004877/91‑3 (MQ=25) aGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAAT‑TCACATCATGTGAGATTGCTAGttt < 1:113524/91‑3 (MQ=25) gggAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGtata > 1:2047377/1‑91 (MQ=255) gggAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAAT‑TCACATCATGTGAGATTGCTAGtttt < 1:3863674/91‑4 (MQ=25) gggAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAAT‑TCACATCATGTGAGATTGCTAGtttt < 1:577671/91‑4 (MQ=25) gAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGtatata > 2:1713711/1‑91 (MQ=255) aaGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATTTAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTatatat > 2:2563616/1‑91 (MQ=255) aaGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTatatat < 2:1767990/91‑1 (MQ=255) aGGGTGAGATAATTTTTTGTATCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATc > 2:1877862/1‑91 (MQ=255) aGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATc > 1:3259029/1‑91 (MQ=255) gTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGt < 2:1988455/91‑1 (MQ=255) gagaTAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTAc > 1:1832055/1‑91 (MQ=255) agaTAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACa > 2:1786989/1‑91 (MQ=255) aaTTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAattt > 1:2540473/1‑91 (MQ=255) aTTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAattta > 1:3511419/1‑91 (MQ=255) ttttttGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAatttat > 1:2829220/1‑91 (MQ=255) ttttttGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAatttat < 2:3770660/91‑1 (MQ=255) tttttGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAatttatt > 2:1177127/1‑91 (MQ=255) tttttGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAatttatt < 1:2727889/91‑1 (MQ=255) tttttGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAatttatt > 2:1241379/1‑91 (MQ=255) tttttGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAatttatt < 1:258684/91‑1 (MQ=255) ttttGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAatttattt > 1:3567931/1‑91 (MQ=255) tttGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAAtttattta > 2:2300579/1‑91 (MQ=255) ttcgTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAAtttattta > 2:2961047/4‑91 (MQ=255) ttGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATttaa > 2:3190031/1‑91 (MQ=255) ttGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTACttaa > 2:1643488/1‑91 (MQ=255) ttGTACCAAAATGAAAATATTTTTAAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATttaa < 2:1737631/91‑1 (MQ=255) tGTACCAAAATGAAAATTTTTTTCAAAAATTAACAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTACttaat > 2:3145839/1‑91 (MQ=255) tGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATttaat > 1:374143/1‑91 (MQ=255) tGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATttaat > 2:2832030/1‑91 (MQ=255) tACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATttaatta > 2:2341526/1‑91 (MQ=255) tACCAAAATGAAAATTTAAAAAAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATttaatta < 2:766301/91‑1 (MQ=255) aCCAAAATGAAAATTTTTTTCAAAAATAAAAAGCTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATttaattaa < 1:1043044/91‑1 (MQ=255) aCCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATttaattaa > 2:1370848/1‑91 (MQ=255) ccAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTtaattaat > 1:2677545/1‑91 (MQ=255) ccAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTtaattaat > 2:3867774/1‑91 (MQ=255) aaaaTGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATgg < 1:1284652/91‑1 (MQ=255) aTGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATg > 1:2204592/1‑91 (MQ=255) gAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGaa < 2:367244/91‑1 (MQ=255) gAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGaa < 1:76967/91‑1 (MQ=255) aaaaTTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAAt > 1:2480514/1‑91 (MQ=255) aaTTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGc < 1:1878627/91‑1 (MQ=255) aTTTTTTTCAAAAATAAAAAACTAGCAATCTCACATCATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCa < 2:3055291/91‑1 (MQ=255) tttttttCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGtat > 1:3618057/1‑53 (MQ=255) tttttttCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGtat < 2:3618057/53‑1 (MQ=255) tttttttCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCAt < 1:1422688/91‑1 (MQ=255) tttttttCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCAt < 2:3217178/91‑1 (MQ=255) ttttCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGc < 1:2961085/91‑1 (MQ=255) ttCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTa > 1:2935161/1‑91 (MQ=255) tCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAg < 1:1103359/91‑1 (MQ=255) tCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAg < 2:3347991/91‑1 (MQ=255) ttttaaaaCTAGCAATCTCACATGATGTTA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGATTGCATAGCTAGAAACtt > 2:1253179/5‑91 (MQ=255) tAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTc < 2:728359/91‑1 (MQ=255) aaaaaaCTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCt < 2:1473577/91‑1 (MQ=255) aaaaaCTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTg < 1:806979/91‑1 (MQ=255) aaaaaCTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTg > 1:1642269/1‑91 (MQ=255) aaaCTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGaa < 2:3828094/91‑1 (MQ=255) aaaCTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGaa > 1:981240/1‑91 (MQ=255) aaaCTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGaa > 2:2789589/1‑91 (MQ=255) aaaCTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGaa > 1:1177883/1‑91 (MQ=255) aaCTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAg < 2:3259928/91‑1 (MQ=255) aaCTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAg > 2:215381/1‑91 (MQ=255) aCTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGc < 2:383311/91‑1 (MQ=255) cTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCt < 1:92859/91‑1 (MQ=255) tAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTg < 1:2267215/91‑1 (MQ=255) aGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTgg < 2:1681655/91‑1 (MQ=255) cAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGaa > 2:2264495/1‑91 (MQ=255) aaTCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAAt < 2:654415/91‑1 (MQ=255) cacaTGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAATTGTAc < 1:1256149/91‑1 (MQ=255) cacaTCATGTGAGATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAATTGTa > 2:2922697/1‑91 (MQ=255) caTGATGTGA‑ATTGCTGGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAATTGTACgg > 1:2632000/1‑91 (MQ=255) tgatgTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAATTGTACGGTa < 2:3457055/91‑1 (MQ=255) gatgTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAATTGTACGGTAg < 2:106136/91‑1 (MQ=255) | TATAAATATGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAATTGTACGGTAG > USA300TCH1516_ALE/740233‑740412 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |