BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	890,015	T→G	5.6%	intergenic (+28/‑130)	mgsR → / → gcvH	Regulatory protein MgsR/Glycine cleavage system H protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	890,015	0	T	G	5.6%	803.0 / 30.0	240	intergenic (+28/‑130)	mgsR/gcvH	Regulatory protein MgsR/Glycine cleavage system H protein
Reads supporting (aligned to +/- strand): ref base T (122/104); new base G (8/6); total (130/110)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 250 total aligned reads displayed.

ATGGGCGATAAGATAACATTAGGATTTAAAGAAGATCAATATAAAGAGACTTGGTTAGCGTAAGTGAAATGTAAGCGTTTACTAAATATCTCGATATTTAGATTCATTACATGTAAAATGAAATAAGCTATACAATTGTTAATTTTTATAAATATAGTTGAATAGCATCTAGCCTTATGGC  >  USA300TCH1516_ALE/889925‑890105

  gggCGATAAGATAACATTAGGATTTAAAGAAGATCAATATAAAGAGACTTGGTTAGCGTAAGTGAAATGTAAGCGTTTACTAAATATCTCg                                                                                          <  1:3627884/91‑1 (MQ=255)

    gCGATAAGATAACATTAGGATTTAAAGAAGATCAATATAAAGAGACTTGGTTAGCGTAAGTGAAATGTAAGCGTTTACTAAATATCTCGat                                                                                        >  1:537040/1‑91 (MQ=255)

    gCGATAAGATAACATTAGGATTTAAAGAAGATCAATATAAAGAGACTTGGTTAGCGTAAGTGAAATGTAAGCGTTTACTAAATATCTCGat                                                                                        >  2:3536604/1‑91 (MQ=255)

     cGATAAGATAACATTGGGATGTAAAGAAGATCAATATAAAGAGACTTGGTTAGCGTAAGTGAAATGTAAGCGTTTACTAAATATCGAGata                                                                                       <  2:2581780/91‑1 (MQ=255)

     cGATAAGATAACATTAGGATTTAAAGAAGATCAATATAAAGAGACTTGGTTAGCGTAAGTGAAATGTAAGCGTTTACTAAATATCTCGata                                                                                       >  2:1050988/1‑91 (MQ=255)

       ataagataaCATTAGGATTTAAAGAAGATCAATATAAAGAGACTTGGTTAGCGTAAGTGAAATGTAAGCGTTTACTAAATATCTCGATAtt                                                                                     <  1:1966552/91‑1 (MQ=255)

        taagataaCATTAGGATTTAAAGAAGATCAATATAAAGAGACTTGGTTAGCGTAAGTGAAATGTAAGCGTTTACTAAATATCTCGATAttt                                                                                    <  1:2554602/91‑1 (MQ=255)

        taagataaCATTAGGATTTAAAGAAGATCAATATAAAGAGACTTGGTTAGCGTAAGTGAAATGTAAGCGTTTACTAAATATCGAGATAttt                                                                                    <  2:2486187/91‑1 (MQ=255)

           gataaCATTAGGATTTAAAGAAGATCAATATAAAGAGACTTGGTTAGCGTAAGTGAAATGTAAGCGTTTACTAAATATCTCGATATTTAGa                                                                                 <  2:3661473/91‑1 (MQ=255)

           gagaaCATTAGGATTTAAAGAAGATCAATATAAAGAGACTTGGTGAGCGTAAGTGAAATGTAAGCGTTTACTAAATATCTCGATATTTAGa                                                                                 <  2:2326393/88‑1 (MQ=255)

            ataaCATTAGGATTTAAAGAAGATCAATATAAAGAGACTTGGTTAGCGTAAGTGAAATGTAAGCGTTTACTAAATATCTCGATATTTAGAt                                                                                >  2:3448949/1‑91 (MQ=255)

               aCATTAGGATTTAAAGAAGATCAATATAAAGAGACTTGGTTAGCGTAAGTGAAATGTAAGCGTTTACTAAATATCTCGATATTTAGATTCa                                                                             <  2:2373721/91‑1 (MQ=255)

               aCATTAGGATTTAAAGAAGATCAATATAAAGAGACTTGGTTAGCGTAAGTGAAATGTAAGCGTTTACTAAATATCTCGATATTTAGATTCa                                                                             >  2:660316/1‑91 (MQ=255)

                  ttAGGATTTAAAGAAGATGAATTGAAAGAGACTTGGTTAGCGTAAGTGAAATGTACGCGTTTACTAAATATCTCGATATTTAGATTCATTa                                                                          <  1:54456/91‑1 (MQ=255)

                    aGGATTTAAAGAAGATCAATATAAAGAGACTTGGTTAGCGTAAGTGAAATGTAAGCGTTTACTAAATATCTCGATATTTAGATTCATTACa                                                                        >  1:1724014/1‑91 (MQ=255)

                     ggATTTAAAGAAGATCAATATAAAGAGACTTGGTTAGCGTAAGTGAAATGTAAGCGTTTACTAAATATCTCGATATTTAGATTCATTACAt                                                                       >  2:3036597/1‑91 (MQ=255)

                       aTTTAAAGAAGATCAATATAAAGAGACTTGGTTAGCGTAAGTGAAATGTAAGCGTTTACTAAATATCTCGATATTTAGATTCATTACATGt                                                                     <  1:3326586/91‑1 (MQ=255)

                       aTTTAAAGAAGATCAATATAAAGAGACTTGGTTAGCGTAAGTGAAATGTAAGCGTTTACTAAATATCTCGATATTTAGATTCATTACATGt                                                                     <  2:2651396/91‑1 (MQ=255)

                       aTTTAAAGAAGATCAATATAAAGAGACTTGGTTAGCGTAAGTGAAATGTAAGCGTTTACTAAATATCTCGATATTTAGATTCATTACATGt                                                                     >  1:2459788/1‑91 (MQ=255)

                         ttAAAGAAGATCAATATAAAGAGACTTGGTTAGCGTAAGTGAAATGTAAGCGTTTACTAAATATCTCGATATTTAGATTCATTACATGTaa                                                                   >  2:2256054/1‑91 (MQ=255)

                           aaaGAAGATCAATATAAAGAGACTTGGTTAGCGTAAGTGAAATGTAAGCGTTTACTAAATATCTCGATATTTAGATTCATTACATGTaaaa                                                                 <  1:1143057/91‑1 (MQ=255)

                               aagaTCAATATAAAGAGACTTGGTTAGCGTAAGTGAAATGTAAGCGTTTACTAAATATCTCGATATTTAGATTCATTACATGTAAAATGaa                                                             >  2:3276481/1‑91 (MQ=255)

                                agaTCAATATAAAGAGACTTGGTTAGCGTAAGTGAAATGTAAGCGTTTACTAAATATCTCGATATTTAGATTCATTACATGTAAAATGaaa                                                            >  1:1602112/1‑91 (MQ=255)

                                 gaTCAATATAAAGAGACTTGGTTAGCGTAAGTGAAATGTAAGCGTTTACTAAATATCTCGATATTTAGATTCATTACATGTAAAATGAAAt                                                           >  2:434495/1‑91 (MQ=255)

                                 gaTCAATATAAAGAGACTTGGTTAGCGTAAGTGAAATGTAAGCGTTTACTAAATATCTCGATATTTAGATTCATTACATGTAAAATGAAAt                                                           >  2:2452492/1‑91 (MQ=255)

                                    cAATATAAAGAGACTTGGTTAGCGTAAGTGAAATGTAAGCGTTTACTAAATATCTCGATATTTAGATTCATTACATGTAAAATGAAATAAg                                                        >  2:149688/1‑91 (MQ=255)

                                     aaTATAAAGAGACTTGGTTAGCGTAAGTGAAATGTAAGCGTTTACTAAATATCTCGATATTTAGATTCATTACATGTAAAATGAAATAAGc                                                       >  1:1793334/1‑91 (MQ=255)

                                      atatAAAGAGACTTGGTTAGCGTAAGTGAAATGTAAGCGTTTACTAAATATCTCGATATTTAGATTCATTACATGTAAAATGAAATAAGCt                                                      <  2:349030/91‑1 (MQ=255)

                                       tataAAGAGACTTGGTTAGCGTAAGTGAAATGTAAGCGTTTACTAAATATCTCGATATTTAGATTCATTACATGTAAAATGAAATAAGCta                                                     >  1:2180367/1‑91 (MQ=255)

                                        ataAAGAGACTTGGTTAGCGTAAGTGAAATGTAAGCGTTTACTAAATATCTCGATATTTAGATTCATTACATGTAAAATGAAATAAGCtat                                                    <  1:3774924/91‑1 (MQ=255)

                                          aaaGAGACTTGGTTAGCGTAAGTGAAATGTAAGCGTTTACTAAATATCTCGATATTTAGATTCATTACATGTAAAATGAAATAAGCTATAc                                                  <  1:1516839/91‑1 (MQ=255)

                                          aaaGAGACTTGGTTAGCGTAAGTGAAATGTAAGCGTTTACTAAATATCTCGATATTTAGATTCATTACATGTAAAATGAAATAAGCTATAc                                                  >  1:71436/1‑91 (MQ=255)

                                             gagaCTTGGTTAGCGTAAGTGAAATGTAAGCGTTTACTAAATATCTCGATATTTAGATTCATTACATGTAAAATGAAATAAGCTATACAAt                                               <  2:3287315/91‑1 (MQ=255)

                                              agaCTTGGTTAGCGTAAGTGAAATGTAAGCGTTTACTAAATATCTCGATATTTAGATTCATTACATGCAAAATGAAATAAGCTATACAAtt                                              <  2:2853104/91‑1 (MQ=255)

                                               gaCTTGGTTAGCGTAAGTGAAATGTAAGCGTTTACTAAATATCTCGATATTTAGATTCATTACATGTAAAATGAAATAAGCTATACAATTg                                             <  1:3216143/91‑1 (MQ=255)

                                               gaCTTGGTTAGCGTAAGTGAAATGTAAGCGTTTACTAAATATCTCGATATTTAGATTCATTACATGTAAAATGAAATAAGCTATACAATTg                                             <  1:472536/91‑1 (MQ=255)

                                                aCTTGGTTAGCGTAAGTGAAATGTAAGCGTTTACTAAATATCTCGATATTTAGATTCATTACATGTAAAATGAAATAAGCTATACAATTGt                                            <  2:478349/91‑1 (MQ=255)

                                                  ttGGTTAGCGTAAGTGAAATGTAAGCGTTTACTAAATATCTCGATATTTAGATTCATTACATGTAAAATGAAATAAGCTATACAATTGTTa                                          >  1:606320/1‑91 (MQ=255)

                                                   tGGTTAGCGTAAGTGAAATGTAAGCGTTTACTAAATATCTCGATATTTAGATTCATTACATGTAAAATGAAATAAGCTATACAATTGTTaa                                         >  1:2518364/1‑91 (MQ=255)

                                                   tGGTTAGCGTAAGTGAAATGTAAGCGTTTACTAAATATCTCGATATTTAGATTCATTACATGTAAAATGAAATAAGCTATACAATTGTTaa                                         >  1:3783375/1‑91 (MQ=255)

                                                    ggTTAGCGTAAGTGAAATGTAAGCGTTTACTAAATATCTCGATATTTAGATTCATTACATGTAAAATGAAATAAGCTATACAATTGTTAAt                                        >  1:2531473/1‑91 (MQ=255)

                                                    ggTTAGCGTAAGTGAAATGTAAGCGTTTACTAAATATCTCGATATTTAGATTCATTACATGTAAAATGAAATAAGCTATACAATTGTTAAt                                        >  2:1983853/1‑91 (MQ=255)

                                                    ggTTAGCGTAAGTGAAATGTAAGCGTTTACTAAATATCTCGATATTTAGATTCATTACATGTAAAATGAAATAAGCTATACAATTGTTAAt                                        >  1:732156/1‑91 (MQ=255)

                                                    ggTTAGCGTAAGTGAAATGTAAGCGTTTACTAAATATCTCGATATTTAGATTCATTACATGTAAAATGAAATAAGCTATACAATTGTTAAt                                        >  2:3729288/1‑91 (MQ=255)

                                                     gTTAGCGTAAGTGAAATGTAAGCGTTTACTAAATATCTCGATATTTAGATTCATTACATGTAAAATGAAATAAGCTATACAATTGTTAAtt                                       >  2:3875056/1‑91 (MQ=255)

                                                     gTTAGCGTAAGTGAAATGTAAGCGTTTACTAAATATCTCGATATGTAGATTCATTACATGTAAAATGAAATAAGCTATACAATTGTTAAtt                                       <  1:3078547/91‑1 (MQ=255)

                                                       tAGCGTAAGTGAAATGTAAGCGTTTACTAAATATCTCGATATTTAGATTCATTACATGTAAAATGAAATAAGCTATACAATTGTTAAtttt                                     <  1:3729850/91‑1 (MQ=255)

                                                       tAGCGTAAGTGAAATGTAAGCGTTTACTAAATATCTCGATATTTAGATTCATTACATGTAAAATGAAATAAGCTATACAATTGTTAAtttt                                     <  1:897704/91‑1 (MQ=255)

                                                        aGCGTAAGTGAAATGTAAGCGTTTCCTAAATATCTCGATATTTAGATTCATTACATGTAAAATGAAATAAGCTATACAATTGTTAAttttt                                    <  2:3597932/91‑1 (MQ=255)

                                                         gCGTAAGTGAAATGTAAGCGTTTACTAAATATCTCGATATTTAGATTCATTACATGTAAAATGAAATAAGCTATACAATTGTTAATTTTta                                   <  1:3875056/91‑1 (MQ=255)

                                                         gCGTAAGTGAAATGTAAGCGTTTACTAAATATCTCGATATTTAGATTCATTACATGTAAAATGAAATAAGCTATACAATTGTTAATTTTta                                   >  1:2708104/1‑91 (MQ=255)

                                                         gCGTAAGTGAAATGTAAGCGTTTACTAAATATCTCGATATTTAGATTCATTACATGTAAAATGAAATAAGCTATACAATTGTTAATTTTta                                   >  2:1173559/1‑91 (MQ=255)

                                                         gCGGAAGGGAAATGTAAGCGTTTACTTAATATCTCGATATTTAGATTCATTACATGTAAAATGAAATAAGCTATACAATTGTTAATTTTta                                   >  2:1313134/1‑91 (MQ=255)

                                                           gTAAGTGAAATGTAAGCGTTTACTAAATATCTCGATATTTAGATTCATTACATGTAAAATGAAATAAGCTATACAATTGTTAATTTTtata                                 >  2:3237210/1‑91 (MQ=255)

                                                           gTAAGTGAAATGTAAGCGTTTACTAAATATCTCGATATTTAGATTCATTACATGTAAAATGAAATAAGCTATACAATTGTTAATTTTtata                                 >  1:2048694/1‑91 (MQ=255)

                                                           gTAAGTGAAATGTAAGCGTTTACTAAATATCTCGATATTTAGATTCATTACATGTAAAATGAAATAAGCTATACAATTGTTAATTTTtata                                 <  2:2167986/91‑1 (MQ=255)

                                                           gTAAGTGAAATGTAAACGTTTACTAAATATCTCGATATTTAGATTCATTACATGTAAAATGAAATAAGCTATACAATTGTTAATTTTtata                                 >  2:1840032/1‑91 (MQ=255)

                                                              aGTGAAATGTAAGCGTTTACTAAATATCTCGATATTTAGATTCATTACATGTAAAATGAAATAAGCTATACAATTGTTAATTTTTATAAat                              >  1:1036395/1‑91 (MQ=255)

                                                              aGTGAAATGTAAGCGTTTACTAAATATCTCGATATTTAGATTCATTACATGTAAAATGAAATAAGCTATACAATTGTTAATTTTTATAAat                              >  2:3332292/1‑91 (MQ=255)

                                                              aGTGAAATGTAAGCGTTTACTAAATATCTCGATATTTAGATTCATTACATGTAAAATGAAATAAGCTATACAATTGTTAATTTTTATAAat                              >  1:2737073/1‑91 (MQ=255)

                                                               gTGAAATGTAAGCGTTTACTAAATATCTCGATATTTAGATTCATTACATGTAAAATGAAATAAGCTATACAATTGTTAATTTTTATAAata                             >  1:2666676/1‑91 (MQ=255)

                                                                tGAAATGTAAGCGTTTACTAAATATCTCGATATTTAGATTCATTACATGTAAAATGAAATAAGCTATACAATTGTTAATTTTTATAAatat                            <  1:1008976/91‑1 (MQ=255)

                                                                  aaaTGTAAGCGTTTACTAAATATCTCGATATTTAGATTCATTACATGTAAAATGAAATAAGCTATACAATTGTTAATTTTTATAAATATAg                          <  1:2211720/91‑1 (MQ=255)

                                                                  aaaTGTAAGCG‑TTACTAAATATCTCGATATTTAGATTCATTACATGTAAAATGAAATAAGCTATACAATTGTTAATTTTTATAAATATAGt                         <  2:533809/91‑1 (MQ=255)

                                                                   aaTGTAAGCGTTTACTAAATATCTCGATATTTAGATTCATTACATGTAAAATGAAATAAGCTATACAATTGTTAATTTTTATAAAtatatt                         >  1:3051602/1‑89 (MQ=255)

                                                                   aaTGTAAGCGTTTACTAAATATCTCGATATTTAGATTCATTACATGTAAAATGAAATAAGCTATACAATTGTTAATTTTTATAAATATAGt                         >  1:1913565/1‑91 (MQ=255)

                                                                    aTGTAAGCGTTTACTAAATATCTCGATATTTAGATTCATTACATGTAAAATGAAATAAGCTATACAATTGTTAATTTTTATAAATATAGtt                        >  1:781707/1‑91 (MQ=255)

                                                                     tGTAAGCGTTTACTAAATATCTCGATATTTAGATTCATTACATGTAAAATGAAATAAGCTATACAATTGTTAATTTTTATAAATATAGTTg                       >  2:2891060/1‑91 (MQ=255)

                                                                     tGTAAGCGTTTACTAAATATCTCGATATTTAGATTCATTACATGTAAAATGAAATAAGCTATACAATTGTTAATTTTTATAAATATAGTTg                       <  2:2159874/91‑1 (MQ=255)

                                                                     tGTAAGCGTTGACTATATATCTCGATATTTAGATTCATTACATGTAAAATGAAATAAGCTATACAATTGTTAATTTTTATAAATATAGTTg                       <  2:1128427/91‑1 (MQ=255)

                                                                      gTAAGCGTTTACTAAATATCTCGATATTTAGATTCATTACATGTAAAATGAAATAAGCTATACAATTGTTAATTTTTATAAATATAGTTGa                      >  1:2870588/1‑91 (MQ=255)

                                                                      gTAAGCGTTTACTAAATATCTCGATATTTAGATTCATTACATGTAAAATGAAATAAGCTATACAATTGTTAATTTTTATAAATATAGTTGa                      >  1:183176/1‑91 (MQ=255)

                                                                       tAAGCGTTTACTAAATATCTCGATATTTAGATTCATTACATGTAAAATGAAATAAGCTATACAATTGTTAATTTTTATAAATATAGTTGaa                     >  2:918619/1‑91 (MQ=255)

                                                                       tAAGCGTTTACTAAATATCTCGATATTTAGATTCATTACATGTAAAATGAAATAAGCTATACAATTGTTAATTTTTATAAATATAGTTGaa                     <  1:1409226/91‑1 (MQ=255)

                                                                        aaGCGTTTACTAAATATCTCGATATTTAGATTCATTACATGTAAAATGAAATAAGCTATACAATTGTTAATTTTTATAAATATAGTTGAAt                    <  2:3404636/91‑1 (MQ=255)

                                                                         aGCGTTTACTAAATATCTCGATATTTAGATTCATTACATGTAAAATGAAATAAGCTATACAATTGTTAATTTTTATAAATATAGTTGAATa                   >  1:2973440/1‑91 (MQ=255)

                                                                         aGCGTTTACTAAATATCTCGATATTTAGATTCATTACATGTAAAATGAAATAAGCTATACAATTGTTAATTTTTATAAATATAGTTGAATa                   <  1:3580215/91‑1 (MQ=255)

                                                                          gCGTTTACTAAATATCTCGATATTTAGATTCATTACATGTAAAATGAAATAAGCTATACAATTGTTAATTTTTATAAATATAGTTGAATAg                  <  2:314135/91‑1 (MQ=255)

                                                                          gCGTTTACTAAATATCTCGATACTTAGATTCATTACATGTAAAATGAAATAAGCTATACAATTGTTAATTTTTATAAATATAGTTGAATAg                  <  2:3449265/91‑1 (MQ=255)

                                                                           cGTTTACTAAATATCTCGATATTTAGATTCATTACATGTAAAATGAAATAAGCTATACAATTGTTAATTTTTATAAATATAGTTGAATAGc                 >  2:1476739/1‑91 (MQ=255)

                                                                            gTTTACTAAATATCTCGATATTTAGATTCATTACATGTAAAATGAAATAAGCTATACAATTGTTAATTTTTATAAATATAGTTGAATAGCa                <  1:3595915/91‑1 (MQ=255)

                                                                              ttACTAAATATCTCGATATTTAGATTCATTACATGTAAAATGAAATAAGCTATACAATTGTTAATTTTTATAAATATAGTTGAATAGCATc              <  2:2196185/91‑1 (MQ=255)

                                                                               tACTAAATATCTCGATATTTAGATTCATTACATGTAAAATGAAATAAGCTATACAATTGTTAATTTTTATAAATATAGTTGAATAGCATCt             >  1:587947/1‑91 (MQ=255)

                                                                               atcTAAATATCGAGATATTTAGATTCATTACATGTAAAATGAAATAAGCTATACAATTGTTAATTTTTATAAATATAGTTGAATAGCATCt             >  2:548634/3‑91 (MQ=255)

                                                                               atcTAAATATCGAGATATTTAGATTCATTACATGTAAAATGAAATAAGCTATACAATTGTTAATTTTTATAAATATAGTTGAATAGCATCt             >  1:1561154/3‑91 (MQ=255)

                                                                                aCTAAATATCTCGATATTTAGATTCATTACATGTAAAATGAAATAAGCTATACAATTGTTAATTTTTATAAATATAGTTGAATATCTTCTa            >  2:1698150/1‑91 (MQ=255)

                                                                                 cTAAATATCTCGATATTTAGATTCATTACATGTAAAATGAAATAAGCTATACAATTGTTAATTTTTATAAATATAGTTGAATAGCATCTAg           >  2:2016392/1‑91 (MQ=255)

                                                                                 cTAAATATCTCGATATTTAGATTCATTACATGTAAAATGAAATAAGCTATACAATTGTTAAGTTTTATAAATATAGTTGAATAGCATCTAg           <  1:124433/91‑1 (MQ=255)

                                                                                   aaaTATCTCGATATTTAGATTCATTACATGTAAAATGAAATAAGCTATACAATTGTTAATTTTTATAAATATAGTTGAATAGCATCTAGcc         >  2:1465879/1‑91 (MQ=255)

                                                                                   aaaTATCTCGATATTTAGATTCATTACATGTAAAATGAAATAAGCTATACAATTGTTAATTATTATAAATATAGTTGAATAGCATCTAGcc         >  1:3110947/1‑91 (MQ=255)

                                                                                   aaaTATCGAGATATTTAGATTCATTACATGTAAAATGAAATAAGCTATACAATTGTTAATTTTTATAAATATAGTTGAATAGCATCTAGcc         >  1:2239566/1‑91 (MQ=255)

                                                                                    aaTATCTCGATATTTAGATTCATTACATGTAAAATGAAATAAGCTATACAATTGTTAATTTTTATAAATATAGTTGAATAGCATCTAGCCt        <  1:1313134/91‑1 (MQ=255)

                                                                                    aaTATCGAGATATTTAGATTCATTACATGTAAAATGAAATAAGCTATACAATTGTTAATTTTTATAAATATAGTTGAATAGCATCTAGCCt        >  2:2706616/1‑91 (MQ=255)

                                                                                     atatCTCGATATTTAGATTCATTACATGTAAAATGAAATAAGCTATACAATTGTTAATTTTTATAAATATAGTTGAATAGCATCTAGCCtt       <  2:2566552/91‑1 (MQ=255)

                                                                                      tatCTCGATATTTAGATTCATTACATGTAAAATGAAATAAGCTATACGATTGTTAATTTTTATAAATATAGTTGAATAGCATCTAGCCTTa      <  2:2997446/91‑1 (MQ=255)

                                                                                      tatCTCGATATTTAGATTCATTACATGTAAAATGAAATAAGCTATACGATTGTTAATTTTTATAAATATAGTTGAATAGCATCTAGCCTTa      <  2:2997007/91‑1 (MQ=255)

                                                                                      tatCTCGATATTTAGATTCATTACATGTAAAATGAAATAAGCTATACAATTGTTAATTTTTATAAATATAGTTGAATAGCATCTAGCCTTa      <  2:3640417/91‑1 (MQ=255)

                                                                                        tctcGATATTTAGATTCATTACATGTAAAATGAAATAAGCTATACAATTGTTAATTTTTATAAATATAGTTGAATAGCATCTAGCCTTATg    <  1:2833719/91‑1 (MQ=255)

                                                                                         ctcGATATTTAGATTCATTACATGTAAAATGAAATAAGCTATACAATTGTTAATTTTTATAAATATAGTTGAATAGCATCTAGCCTTATgg   >  1:2403408/1‑91 (MQ=255)

                                                                                          tcGATATTTAGATTCATTACATGTAAAATGAAATAAGCTATACAATTGTTAATTTTTATAAATATAGTTGAATAGCATCTAGCCTTATGGc  <  2:1002163/91‑1 (MQ=255)

ATGGGCGATAAGATAACATTAGGATTTAAAGAAGATCAATATAAAGAGACTTGGTTAGCGTAAGTGAAATGTAAGCGTTTACTAAATATCTCGATATTTAGATTCATTACATGTAAAATGAAATAAGCTATACAATTGTTAATTTTTATAAATATAGTTGAATAGCATCTAGCCTTATGGC  >  USA300TCH1516_ALE/889925‑890105

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`