| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 954,771 | T→C | 5.5% | intergenic (+425/+78) | gluD → / ← glpQ_1 | NAD‑specific glutamate dehydrogenase/Glycerophosphodiester phosphodiesterase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 954,771 | 0 | T | C | 5.5% | 718.5 / 19.6 | 217 | intergenic (+425/+78) | gluD/glpQ_1 | NAD‑specific glutamate dehydrogenase/Glycerophosphodiester phosphodiesterase |
| Reads supporting (aligned to +/- strand): ref base T (87/118); new base C (3/9); total (90/127) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.67e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 231 total aligned reads displayed.
ATAGTGTGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTAATGACT > USA300TCH1516_ALE/954681‑954861 | aTAGTGTGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAAttt < 1:1086803/91‑1 (MQ=255) tAGTGTGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTc < 1:3434182/91‑1 (MQ=255) gtgtGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCaa > 1:2064953/1‑91 (MQ=255) gtgtGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCaa < 1:1626603/91‑1 (MQ=255) tgtgCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATACTTTCAAt > 1:1943694/1‑91 (MQ=255) gtgCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAAtt < 2:399124/91‑1 (MQ=255) tgCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAAttt > 2:2883187/1‑91 (MQ=255) tgCAAGTTGGGGTGGGACGACGAAATAAACTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAAttt > 2:3383264/1‑91 (MQ=255) gCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTg < 1:997148/91‑1 (MQ=255) cAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTgg > 1:648553/1‑91 (MQ=255) cAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTgg > 1:3365198/1‑91 (MQ=255) cAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTgg < 1:2849105/91‑1 (MQ=255) gTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGaaa < 1:3177202/91‑1 (MQ=255) ttGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAat < 2:2712444/91‑1 (MQ=255) tGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAata > 1:2443792/1‑91 (MQ=255) gggTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATc > 2:1123536/1‑91 (MQ=255) ggTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCt < 1:1042262/91‑1 (MQ=255) gggACGACGAACTAAATTTTGCTTAAATATCATTTCTGTCCCACTCCCAGAAAGACAGCGGTAGGATAATTTCAATTTGGAAATATCTTAc < 2:2955169/91‑1 (MQ=25) gggACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTAc > 1:37163/1‑91 (MQ=255) gggACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTAc < 1:1602072/91‑1 (MQ=255) gggACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAAGATATTTCCAAATTGAAATTATCCTAc < 1:703619/91‑1 (MQ=17) ggACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTAct < 2:1308582/91‑1 (MQ=255) gacgacGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTActg < 1:476536/91‑1 (MQ=255) gacgacGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTActg < 1:2504114/91‑1 (MQ=255) acgacgAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTActgc < 2:383223/91‑1 (MQ=255) acgacgAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAAGATATTTCCAAATTGAAATTATCCTActgc < 1:1406997/91‑1 (MQ=17) cgacgaAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTActgct < 2:830512/91‑1 (MQ=255) gacgaAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAAACAGCAGTAAGATATTTCCAAATTGAAATTATCCTActgctg < 1:1116217/91‑1 (MQ=14) gacgaAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAAACAGCAGTAAGATATTTCCAAATTGAAATTATCCTActgctg < 2:3416183/91‑1 (MQ=17) gacgaAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAAACAGCAGTAAGATATTTCCAAATTGAAATTATCCTActgctg < 1:1173277/91‑1 (MQ=14) acgaAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGt < 2:612840/91‑1 (MQ=255) acgaAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGt < 1:3032216/91‑1 (MQ=255) acgaAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGt < 2:1386820/91‑1 (MQ=255) cgaAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAAACAGCAGTAAGATATTTCCAAATTGAAATTATCCTACTGCTGtc < 1:578566/91‑2 (MQ=14) gaAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGttt > 1:1567309/1‑91 (MQ=255) aataaatTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGttttt < 1:1616603/91‑1 (MQ=255) aataaatTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGttttt < 2:3356629/91‑1 (MQ=255) ataaatTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGtttttt > 2:3673818/1‑91 (MQ=255) ataaatTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGtttttt > 1:2541517/1‑91 (MQ=255) ataaatTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGtttttt > 1:2197213/1‑91 (MQ=255) taaatTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTta > 2:3248829/1‑91 (MQ=255) taaatTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTta > 1:2364622/1‑91 (MQ=255) aaatTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTtat < 2:2208651/91‑1 (MQ=255) aatTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTtata > 2:2165894/1‑91 (MQ=255) aatTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTtata < 1:1202988/91‑1 (MQ=255) gCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATa > 1:2776896/1‑91 (MQ=255) gCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGAAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATa < 2:641521/91‑1 (MQ=255) cGAAAATATCATTTCTGTCCCACTACCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAg < 2:3165808/91‑1 (MQ=255) aaTATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCtat > 1:664470/1‑91 (MQ=255) atatCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCtata < 2:1263641/91‑1 (MQ=255) atatCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCtata < 1:2457017/91‑1 (MQ=255) tCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATAttt > 1:2899614/1‑91 (MQ=255) tCATTTCTGTCACACTCCCAAAAAGACAGCAGTAGGATAATGTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATAttt < 2:1569757/91‑1 (MQ=255) aTTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATAttttt > 2:3512827/1‑91 (MQ=255) aTTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATAttttt > 2:2425043/1‑91 (MQ=255) aTTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATAttttt > 2:318555/1‑91 (MQ=255) ttCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATCTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGt < 1:3868577/91‑1 (MQ=255) tGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTAtt < 2:1007042/91‑1 (MQ=255) tGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTAtt < 1:1215232/91‑1 (MQ=255) aCTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGtt < 1:1895771/91‑1 (MQ=255) tCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGtttt < 1:354181/91‑1 (MQ=255) cccAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTc > 2:1790864/1‑91 (MQ=255) ccAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCt > 2:2969917/1‑91 (MQ=255) ccAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCt < 2:1980982/91‑1 (MQ=255) ccAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCt > 1:1701717/1‑91 (MQ=255) cAAAAAGACAGCAGTAGGATACTTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTa > 2:1546322/1‑91 (MQ=255) cAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTa < 2:1934941/91‑1 (MQ=255) taaaaaaCAGCAGTAAGATATTTCCAAATTGAAATTATCCTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAg > 1:3622235/2‑91 (MQ=14) aaGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGttt < 2:2249663/91‑1 (MQ=255) aGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTa < 2:3641741/91‑1 (MQ=255) gACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTaa < 2:1859851/91‑1 (MQ=255) cagcagTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTc > 2:1802271/1‑81 (MQ=255) cagcagTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACa > 2:776849/1‑91 (MQ=255) cagcagTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACa > 1:1657947/1‑91 (MQ=255) agcagTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACAt < 2:927728/91‑1 (MQ=255) agcagTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACAt > 2:2670256/1‑91 (MQ=255) agcagTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACAt < 1:2571876/91‑1 (MQ=255) agcagTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACAt < 2:1550731/91‑1 (MQ=255) gcagTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACAtt > 1:3248190/1‑91 (MQ=255) cagTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATta < 1:296111/91‑1 (MQ=255) agTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATtac < 1:1790864/91‑1 (MQ=255) agTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATtac > 1:256211/1‑91 (MQ=255) ggATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTactact < 1:1123536/91‑1 (MQ=255) ggATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTactact > 1:958322/1‑91 (MQ=255) gATAATTTCAATTTGGAAATATTTTACTGCTGTTTTTTATAGTAATAGCGATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACtt < 2:2951800/91‑1 (MQ=255) gATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACtt < 1:2044960/91‑1 (MQ=255) aTAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTa < 2:3759106/91‑1 (MQ=255) aTAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTa < 2:480333/91‑1 (MQ=255) aTAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTa > 1:1584935/1‑91 (MQ=255) aaTTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTAAt < 1:3835332/91‑1 (MQ=255) aaTTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTAAt > 2:2018279/1‑91 (MQ=255) aaTTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTAAt > 1:272457/1‑91 (MQ=255) aaTTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTAAt < 2:256211/91‑1 (MQ=255) aaTTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTAAt < 1:2165894/91‑1 (MQ=255) aTTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTAATg < 2:1331681/91‑1 (MQ=255) aTTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTAATg < 2:69277/91‑1 (MQ=255) tttCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTAATGa < 1:890051/91‑1 (MQ=255) tttCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTAATGa > 2:2636501/1‑91 (MQ=255) tttCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTAATGa < 1:2094717/91‑1 (MQ=255) ttCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTAATGAc > 2:2820825/1‑91 (MQ=255) | ATAGTGTGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTAATGACT > USA300TCH1516_ALE/954681‑954861 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |