| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 954,787 | T→C | 5.3% | intergenic (+441/+62) | gluD → / ← glpQ_1 | NAD‑specific glutamate dehydrogenase/Glycerophosphodiester phosphodiesterase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 954,787 | 0 | T | C | 5.3% | 752.3 / 21.8 | 227 | intergenic (+441/+62) | gluD/glpQ_1 | NAD‑specific glutamate dehydrogenase/Glycerophosphodiester phosphodiesterase |
| Reads supporting (aligned to +/- strand): ref base T (93/122); new base C (3/9); total (96/131) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.46e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.88e-01 | |||||||||||
Only 100 of 232 total aligned reads displayed.
GGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTAATGACTTCTTTATATTTATCAG > USA300TCH1516_ALE/954697‑954877 | ggTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCt < 1:1042262/91‑1 (MQ=255) gTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCtt < 1:695138/91‑1 (MQ=255) gTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCtt > 1:246989/1‑91 (MQ=255) gggACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTAc < 2:3359682/91‑1 (MQ=255) gggACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTAc < 1:1602072/91‑1 (MQ=255) acgacgAAATAAATTTTGCGAAGATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTActgc < 2:3809838/91‑1 (MQ=255) acgacgAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTActgc < 2:3428295/91‑1 (MQ=255) acgacgAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAAGATATTTCCAAATTGAAATTATCCTActgc < 2:1248178/91‑1 (MQ=17) acgacgAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAAGATATTTCCAAATTGAAATTATCCTActgc < 1:1406997/91‑1 (MQ=17) cgacgaAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTActgct < 2:830512/91‑1 (MQ=255) cgacgaAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAAGATATTTCCAAATTGAAATTATCCTActgct < 1:3233157/91‑1 (MQ=17) gacgaAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAAACAGCAGTAAGATATTTCCAAATTGAAATTATCCTActgctg < 1:1116217/91‑1 (MQ=14) acgaAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGt < 2:612840/91‑1 (MQ=255) acgaAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGt < 2:1386820/91‑1 (MQ=255) acgaAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGt < 1:3562936/91‑1 (MQ=255) acgaAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGt < 2:3645055/91‑1 (MQ=255) cgaAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGtt > 2:39414/1‑91 (MQ=255) gaAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGttt > 1:1567309/1‑91 (MQ=255) aataaatTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGttttt < 1:1616603/91‑1 (MQ=255) aataaatTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGttttt < 2:3356542/91‑1 (MQ=255) ataaatTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGtttttt > 1:2543958/1‑91 (MQ=255) ataaatTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGtttttt > 1:2541472/1‑91 (MQ=255) aaatTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTtat < 2:2208651/91‑1 (MQ=255) aatTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTtata > 2:2165894/1‑91 (MQ=255) aatTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTtata < 1:1202988/91‑1 (MQ=255) tGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAAt < 1:796148/91‑1 (MQ=255) gCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATa > 1:2776896/1‑91 (MQ=255) gCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGAAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATa < 2:641521/91‑1 (MQ=255) gCGAAAATATCATTTCTGGCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATa < 2:3632779/91‑1 (MQ=255) gAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGc < 2:317614/91‑1 (MQ=255) gAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGc > 1:3394345/1‑91 (MQ=255) aaaTATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCta < 2:2064840/91‑1 (MQ=255) atatCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCtata < 1:2456668/91‑1 (MQ=255) tatCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTatat < 2:1602776/91‑1 (MQ=255) aTTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATAttttt > 2:2374176/1‑91 (MQ=255) aTTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATAttttt > 2:2425043/1‑91 (MQ=255) ttCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATCTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGt < 1:3868577/91‑1 (MQ=255) tGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTAtt < 2:1007042/91‑1 (MQ=255) tCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTa < 1:3018947/91‑1 (MQ=255) tCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTa > 1:1969373/1‑91 (MQ=255) ccACTCCCAAAAAGACTGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATg < 2:3727908/91‑1 (MQ=255) cACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGt > 1:1168468/1‑91 (MQ=255) cccAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTc > 2:1790864/1‑91 (MQ=255) cccAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTc < 1:942276/91‑1 (MQ=255) ccAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCt > 1:1701717/1‑91 (MQ=255) ccAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCt < 2:1980982/91‑1 (MQ=255) cAAAAAGACAGCAGTAGGATACTTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTa > 2:1546322/1‑91 (MQ=255) cAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTa > 2:3101893/1‑91 (MQ=255) cAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTa > 1:2220698/1‑91 (MQ=255) ataaaaaaCAGCAGTAAGATATTTCCAAATTGAAATTATCCTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTa > 1:2937791/3‑91 (MQ=2) aaaaaGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAg > 1:3039689/1‑91 (MQ=255) aaaaGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGt < 2:1567419/91‑1 (MQ=255) aaaGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGtt > 1:2071612/1‑91 (MQ=255) aaGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGttt < 2:2830173/91‑1 (MQ=255) aaGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGttt < 2:2249663/91‑1 (MQ=255) aGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTa < 1:239192/91‑1 (MQ=255) gACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTaa < 2:1859851/91‑1 (MQ=255) aCAGCAGTAGGCTAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAAc < 2:2964001/91‑1 (MQ=255) cagcagTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACa > 1:1657947/1‑91 (MQ=255) agcagTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACAt > 2:714914/1‑91 (MQ=255) agcagTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACAt > 2:2670256/1‑91 (MQ=255) agcagTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACAt < 2:927728/91‑1 (MQ=255) gcagTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACAtt > 2:1825992/1‑91 (MQ=255) gcagTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACAtt > 2:1917310/1‑91 (MQ=255) cagTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATta < 1:969902/91‑1 (MQ=255) cagTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATta > 1:634501/1‑91 (MQ=255) agTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATtac > 1:256231/1‑91 (MQ=255) ggATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTactact < 1:1123536/91‑1 (MQ=255) ggATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTactact > 2:2942857/1‑91 (MQ=255) gATAATTTCAATTTGGAAATATGTTACTGCTGTTTTTTATAGTAATAGCGATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACtt < 2:2952050/91‑1 (MQ=255) aTAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTa < 2:480333/91‑1 (MQ=255) aTAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTa > 1:1585043/1‑91 (MQ=255) aaTTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTAAt > 1:563744/1‑91 (MQ=255) aaTTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTAAt < 1:2165894/91‑1 (MQ=255) aaTTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTAAt < 2:256211/91‑1 (MQ=255) aTTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTAATg < 2:1331681/91‑1 (MQ=255) tttCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTAATGa > 2:2636501/1‑91 (MQ=255) ttCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTAATGAc > 1:2898895/1‑91 (MQ=255) ttCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTAATGAc < 2:3203689/91‑1 (MQ=255) cAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTAATGActt < 1:2067133/91‑1 (MQ=255) aaTTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTAATGActtc > 1:1808469/1‑91 (MQ=255) aaTTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTAATGActtc > 1:3076747/1‑91 (MQ=255) aaTTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTAATGActtc > 1:2660817/1‑91 (MQ=255) aTTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTAATGActtct > 1:3503792/1‑91 (MQ=255) tttGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTAATGActtctt < 2:1302633/91‑1 (MQ=255) ttGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTTGTTTAACATTACTACTTAATGACTTCttt < 2:3381746/91‑1 (MQ=255) ttGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTAATGACTTCttt > 1:3765888/1‑91 (MQ=255) ttGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTAATGACTTCttt > 1:1242340/1‑91 (MQ=255) tGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTAATGACTTCTTta < 2:789940/91‑1 (MQ=255) ggAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTAATGACTTCTTtat > 2:3394144/1‑91 (MQ=255) ggAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTAATGACTTCTTtat > 2:2742868/1‑91 (MQ=255) ggAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTAATGACTTCTTtat < 1:3835510/91‑1 (MQ=255) ggAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTAATGACTTCTTtat < 2:2028801/91‑1 (MQ=255) gAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTAATGACTTCTTtata > 1:1177380/1‑91 (MQ=255) aaaTATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTAATGACTTCTTTatat > 1:1716724/1‑91 (MQ=255) aaTATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTAATGACTTCTTTATAtt > 1:2730709/1‑91 (MQ=255) atatCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTAATGACTTCTTTATAttt > 1:2114572/1‑91 (MQ=255) tatCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTAATGACTTCTTTATATTTa > 1:1214972/1‑91 (MQ=255) cTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTAATGACTTCTTTATATTTATCa > 2:552516/1‑91 (MQ=255) ttACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTAATGACTTCTTTATATTTATCAg > 1:650586/1‑91 (MQ=255) | GGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTAATGACTTCTTTATATTTATCAG > USA300TCH1516_ALE/954697‑954877 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |