| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 1,055,126 | T→G | 11.2% | T352T (ACA→ACC) | patA_2 ← | Putative N‑acetyl‑LL‑diaminopimelate aminotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 1,055,126 | 0 | T | G | 11.2% | 805.0 / 88.4 | 258 | T352T (ACA→ACC) | patA_2 | Putative N‑acetyl‑LL‑diaminopimelate aminotransferase |
| Reads supporting (aligned to +/- strand): ref base T (127/101); new base G (26/3); total (154/104) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.51e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01 | |||||||||||
Only 100 of 262 total aligned reads displayed.
ATTTAAATATTTAGCAAGTCTCTTCATACCTTCTTTTAAAACATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGAAATCGTCATCGGTGATATGTTTAA > USA300TCH1516_ALE/1055036‑1055214 | ttAAATATTTAGCAAGTCTCTTCATACCTTCTTTTAAAACATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACAGAATTCGGt < 2:1503597/91‑1 (MQ=255) tAAATATTTAGCAAGTCTCTTCATACCTTCTTTTAAAACATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTa > 1:2388280/1‑91 (MQ=255) tAAATATTTAGCAAGTCTCTTCATACCTTCTTTTAAAACATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTa > 1:1619903/1‑91 (MQ=255) tAAATATTTAGCAAGTCTCTTCATACCTTCTTTTAAAACATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTATGTa > 1:3237658/1‑91 (MQ=255) aaTATTTAGCAAGTCTCTTCATACCTTCTTTTAAAACATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTaaa > 2:1680132/1‑91 (MQ=255) atatTTAGCAAGTCTCTTCATACCTTCTTTTAAAACATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAg < 1:1010195/91‑1 (MQ=255) atTTAGCAAGTCTCTTCATACCTTCTTTTAAAACATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGa > 1:1063751/1‑91 (MQ=255) ttAGCAAGTCTCTTCATACCTTCTTTTAAAACATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATg < 1:3384104/91‑1 (MQ=255) ttAGCAAGTCTCTTCATACCTTCTTTTAAAACATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATg < 1:3246327/91‑1 (MQ=255) ttAGCAAGTCTCTTCATACCTTCTTTTAAAACATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATg > 2:2730468/1‑91 (MQ=255) aGCAAGTCTCTTCATACCTTCTTTTAAAACATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAg > 2:321210/1‑91 (MQ=255) cAAGTCTCTTCATACCTTCTTTTAAAACATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAgg > 2:2130043/1‑84 (MQ=255) cAAGTCTCTTCATACCTTCTTTTAAAACATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGcc > 1:1066567/1‑91 (MQ=255) cAAGTCTCTTCATACCTTCTTTTAAAACATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGcc > 2:24911/1‑91 (MQ=255) cAAGTCTCTTCATACCTTCTTTTAAAACATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGcc > 2:24154/1‑91 (MQ=255) ttCATACCTTCTTTTAAAACATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGa < 2:1082648/91‑1 (MQ=255) cATACCTTCTTTTAAAACATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGAtt > 2:3050303/1‑91 (MQ=255) cATACCTTCTTTTAAAACATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGAtt > 2:529576/1‑91 (MQ=255) ccTTCTTTTAAAACATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTa > 2:2964760/1‑91 (MQ=255) cttcttTTAAAACATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTaa < 1:2308568/91‑1 (MQ=255) tcttTTAAAACATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAAt > 2:2860323/1‑91 (MQ=255) cttTTAAAACATCCATTTCATAAGCATAGGAAATCCTTACGAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAAtg < 1:1349436/91‑1 (MQ=255) cttTTAAAACATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAAtg < 2:546082/91‑1 (MQ=255) cttTTAAAACATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAAtg < 2:3359265/91‑1 (MQ=255) cttTTAAAACATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAAtg < 1:115395/91‑1 (MQ=255) tttAAAACATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAAtgtg > 1:2166961/1‑91 (MQ=255) tttAAAACATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAAtgtg < 1:3185825/91‑1 (MQ=255) ttAAAACATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATgtgt < 1:2489549/91‑1 (MQ=255) ttAAAACATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATgtgt < 1:489441/91‑1 (MQ=255) tAAAACATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGtt < 1:2542074/91‑1 (MQ=255) tAAAACATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGtt < 1:2699600/91‑1 (MQ=255) tAAAACATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGtt > 1:732400/1‑91 (MQ=255) aaaCATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGa < 2:3594846/91‑1 (MQ=255) aaaCATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGa > 1:1753874/1‑91 (MQ=255) aaCATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGAt > 2:2373427/1‑91 (MQ=255) cATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTc < 1:1543050/91‑1 (MQ=255) aTCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCa > 1:851520/1‑91 (MQ=255) aTCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCa < 2:13914/91‑1 (MQ=255) aTCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCa > 2:745925/1‑91 (MQ=255) ccATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAg > 2:333017/1‑91 (MQ=255) aTTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTa > 2:3085048/1‑91 (MQ=255) tttCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATgtgt < 1:1421152/79‑1 (MQ=255) tttCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATgtgt > 2:1421152/1‑79 (MQ=255) tttCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTaa > 1:3414686/1‑91 (MQ=255) tttCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATCCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTaa > 2:124899/1‑91 (MQ=255) aTAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCg > 1:2897882/1‑91 (MQ=255) aGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGaca < 1:2121083/91‑1 (MQ=255) gCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGacac < 2:2089465/91‑1 (MQ=255) aTAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACaa < 2:2326852/91‑1 (MQ=255) aaTCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCg > 1:528643/1‑91 (MQ=255) aaTCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCg > 2:328987/1‑91 (MQ=255) aaTCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCg < 2:2778116/91‑1 (MQ=255) tCCTTACGAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTAGaa < 2:3437245/91‑1 (MQ=255) tCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGaa < 1:2865800/91‑1 (MQ=255) tCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGaa < 1:2361388/91‑1 (MQ=255) tCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGaa < 1:329050/91‑1 (MQ=255) ccTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGaaa < 1:1680132/91‑1 (MQ=255) ccTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGaaa < 1:1009551/91‑1 (MQ=255) cTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGAAAt < 1:1079542/91‑1 (MQ=255) ttACAAATCCTTTACCGAATTCTGTAAAGGATGTGCCAGGAACTATTACTCATTGTGTTGAGTCAAGTAAAACGACACAATGTTCGAACTc > 2:2600405/1‑91 (MQ=255) tACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGAAATCg > 2:1664332/1‑91 (MQ=255) tACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGAAATCg < 2:437196/91‑1 (MQ=255) aCAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGAAATCGt > 2:3693456/1‑91 (MQ=255) tcaTCCTTTACAGAATTCGGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGAAATCGTCa > 1:3427192/3‑91 (MQ=38) aaTCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGAAATCGTCAt < 1:3853009/91‑1 (MQ=255) aTCCTTTACAGAATTCGGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGAAATCGTCATc > 2:2691160/1‑91 (MQ=255) tCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGAAATGGTCATCg > 1:1947933/1‑91 (MQ=255) ccTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGAAATCGTCATCgg > 1:2383286/1‑91 (MQ=255) ccTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGAAATCGTCATCgg < 1:3334857/91‑1 (MQ=255) tttACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGAAATCGTCATCGGTg > 2:2268864/1‑91 (MQ=255) tttACAGAATTCGGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGAAATCGTCATCGGTg > 2:2011488/1‑91 (MQ=255) ttACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGAAATCGTCATCGGTGa > 1:1507690/1‑91 (MQ=255) ttACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGAAATCGTCATCGGTGa < 1:3685492/91‑1 (MQ=255) ttACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGAAATCGTCATCGGTGa < 1:2674537/91‑1 (MQ=255) ttACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGAAATCGTCATCGGTGa < 2:3801199/91‑1 (MQ=255) tacagAATTCGGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGAAATCGTCATCGGTGat > 1:792782/5‑91 (MQ=255) tacagAATTCGGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGAAATCGTCATCGGTGat > 1:3220025/5‑91 (MQ=255) tacagAATTCGGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGAAATCGTCATCGGTGat > 2:654850/5‑91 (MQ=255) tACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGAAATCGTCATCGGTg < 1:614501/89‑1 (MQ=255) acagAATTCGGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGAAATCGTCATCGGTGata > 2:3695463/4‑91 (MQ=255) acagAATTCGGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGAAATCGTCATCGGTGata > 2:2051187/4‑91 (MQ=255) acagAATTCGGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGAAATCGTCATCGGTGata > 1:3556713/4‑91 (MQ=255) ccGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGAAATCGTCATCGGTGatat < 2:1575707/91‑1 (MQ=255) ccGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGAAATCGTCATCGGTGatat < 2:1175344/91‑1 (MQ=255) cagAATTCGGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGAAATCGTCATCGGTGatat > 1:3076798/3‑91 (MQ=255) agAATTCGGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGAAATCGTCATCGGTGATATg > 1:2034658/2‑91 (MQ=255) agAATTCGGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGAAATCGTCATCGGTGATATg > 2:1086444/2‑91 (MQ=255) agAATTCGGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGAAATCGTCATCGGTGATATg > 2:1612858/2‑91 (MQ=255) agAATTCGGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGAAATCGTCATCGGTGATATg > 1:2174227/2‑91 (MQ=255) gAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGAAATCGTCATCGGTGATATGt > 1:2329435/1‑91 (MQ=255) gAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGAAATCGTCATCGGTGATATGt > 1:1252129/1‑91 (MQ=255) gAATTCGGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGAAATCGTCATCGGTGATATGt > 2:764836/1‑91 (MQ=255) aaTTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGAAATCGTCATCGGTGATATGtt < 1:28149/91‑1 (MQ=255) aTTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGAAATCGTCATCGGTGATATGttt < 1:3829167/91‑1 (MQ=255) aTTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGAAATCGTCATCGGTGATATGttt < 2:19736/91‑1 (MQ=255) aTTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGAAATCGTCATCGGTGATATGttt > 1:1214548/1‑91 (MQ=255) aTTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGAAATCGTCATCGGTGATATGttt > 1:386500/1‑91 (MQ=255) aTTCGGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGAAATCGTCATCGGTGATATGttt < 1:654850/91‑1 (MQ=255) ttCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGAAATCGTCATCGGTGATATGTTTa < 2:3769343/91‑1 (MQ=255) ttCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGAAATCGTCATCGGTGATATGTTTa < 2:80421/91‑1 (MQ=255) | ATTTAAATATTTAGCAAGTCTCTTCATACCTTCTTTTAAAACATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGAAATCGTCATCGGTGATATGTTTAA > USA300TCH1516_ALE/1055036‑1055214 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |