BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	1,219,579	A→T	6.2%	V297V (GTT→GTA)	USA300HOU_RS06070 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,219,579	0	A	T	6.2%	696.0 / 23.4	209	V297V (GTT→GTA)	USA300HOU_RS06070	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (103/93); new base T (7/6); total (110/99)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

Only 100 of 214 total aligned reads displayed.

ATATAACTGTTCAGTATCTTTATTTTTAGACTGCTCATATTCTTCAATCAACTTCGCTAATTTATTTTGATATTTGTACAACTGCTGTTGAACAAATCGAACTAAATCATTCGCACGTTGTTTAACGCGTTCACGTTCGCCACGCGCATCATAAAAACGATCAAGCAAATCATTTAATGAA  >  USA300TCH1516_ALE/1219489‑1219669

atatAACTGTTCAGTATCTTTATTTTTAGACTGCTCATATTCTTCAATCAACTTCGCTAATTTATTTTGATATTTGTACAACTGCTGTTGa                                                                                            >  2:1614319/1‑91 (MQ=255)

 tataACTGTTCAGTATCTTTATTTTTAGACTGCTCATATTCTTCAATCAACTTCGCTAATTTATTTTGATATTTGTACAACTGCTGTTGaa                                                                                           <  2:1247087/91‑1 (MQ=255)

 tataACTGTTCAGTATCTTTATTTTTAGACTGCTCATATTCTTCAATCAACTTCGCTAATTTATTTTGATATTTGTACAACTGCTGTTGaa                                                                                           <  1:1874616/91‑1 (MQ=255)

  ataACTGTTCAGTATCTTTATTTTTAGACTGCTCATATTCTTCAATCAACTTCGCTAATTTATTTTGATATTTGTACAACTGCTGTTGAAc                                                                                          <  1:3565396/91‑1 (MQ=255)

  ataACTGTTCAGTATCTTTATTTTTAGACTGCTCATATTCTTCAATCAACTTCGCTAATTTATTTTGATATTTGTACAACTGCTGTTGAAc                                                                                          <  1:1276517/91‑1 (MQ=255)

  ataACTGTTCAGTATCTTTATTTTTAGACTGCTCATATTCTTCAATCAACTTCGCTAATTTATTTTGATATTTGTACAACTGCTGTTGAAc                                                                                          <  2:3799783/91‑1 (MQ=255)

   taACTGTTCAGTATCTTTATTTTTAGACTGCTCATATTCTTCAATCAACTTCGCTAATTTATTTTGATATTTGTACAACTGCTGTTGAACa                                                                                         <  1:3484712/91‑1 (MQ=255)

   taACTGTTCAGTATCTTTA‑TTTTAGACTGCTCATATTCTTCAATCAACTTCGCTAATTTATTTTGATATTTGTTCAACAGCAGTTGTACaa                                                                                        <  2:3014097/91‑1 (MQ=37)

    aaCTGTTCAGTATCTTTATTTTTAGACTGCTCATATTCTTCAATCAACTTCGCTAATTTATTTTGATATTTGTACAACTGCTGTTGAACaa                                                                                        >  1:2509596/1‑91 (MQ=255)

     aCTGTTCAGTATCTTTATTTTTAGACTGCTCATATTCTTCAATCAACTTCGCTAATTTATTTTGATATTTGTTCAACAGCAGTTGTACaaa                                                                                       <  1:306551/91‑1 (MQ=38)

     aCTGTTCAGTATCTTTATTTTTAGACTGCTCATATTCTTCAATCAACTTCGCTAATTTATTTTGATATTTGTTCAACAGCAGTTGTACaaa                                                                                       <  1:307002/91‑1 (MQ=38)

     aCTGTTCAGTATCTTTATTTTTAGACTGCTCATATTCTTCAATCAACTTCGCTAATTTATTTTGATATTTGTACAACTGCTGTTGAACaaa                                                                                       >  1:2716200/1‑91 (MQ=255)

      cTGTTCAGTATCTTTATTTTTTTTTTTTTTATATTCTTCAATCAACTTCGCTAATTTATTTTGATATTTGTACAACTGCTGTTGAACAAAt                                                                                      >  2:2157067/1‑91 (MQ=255)

         ttCAGTATCTTTATTTTTAGACTGCTCATATTCTTCAATCAACTTCGCTAATTTATTTTGATATTTGTACAACTGCTGTTGAACAAATCGa                                                                                   >  1:1162286/1‑91 (MQ=255)

         ttCAGGATCTGTATTTTGAGACTGCTCATATTCTTCAAGCAACTTCGCTAATTTATTTTGATATGTGTACAACGGCTGTTGAACAAATCGa                                                                                   <  1:3718486/91‑1 (MQ=255)

          tCAGTATCTTTATTTTTAGACTGCTCATATTCTTCAATCAACTTCGCTAATTTATTTTGATATTTGTACAACTGCTGTTGAACAAATCGaa                                                                                  <  2:1579223/91‑1 (MQ=255)

            aGTATCTTTATTTTTAGACTGCTCATATTCTTCAATCAACTTCGCTAATTTATTTTGATATTTGTACAACTGCTGTTGAACAAATCGAACt                                                                                <  1:1368493/91‑1 (MQ=255)

             gTATCTTTATTTTTAGACTGCTCATATTCTTCAATCAACTTCGCTAATTTATTTTGATATTTGTACAACTGCTGTTGAACAAATCGAACTa                                                                               <  2:2417007/91‑1 (MQ=255)

                tCTTTATTTCTAGACTGCTCATATTCTTCAATCAACTTCGCTAATTTATTTTGATATTTGTACAACTGCTGTTGAACAAATCGAACTAAAt                                                                            >  1:1946357/1‑91 (MQ=255)

                 cTTTATTTTTAGACTGCTCATATTCTTCAATCAACTTCGCTAATTTATTTTGATATTTGTACAACTGCTGTTGAACAAATCGAACTAAATc                                                                           <  1:1095161/91‑1 (MQ=255)

                   ttATTTTTAGACTGCTCATTTTCTTCAATCAACTTCGCTAGTTTATTTTGATATTTGTACAACTGCTGTTGAACAAATCGAACTAAATCAt                                                                         <  2:663657/91‑1 (MQ=255)

                     aTTTTTAGACTGCTCATATTCTTCAATCAACTTCGCTAATTTATTTTGATATTTGTGCAACTGCTGTTGAACAAATCGAACTAAATCATTc                                                                       >  1:529543/1‑91 (MQ=255)

                     aTTTTTAGACTGCTCATATTCTTCAATCAACTTCGCTAATTTATTTTGATATTTGTACAACTGCTGTTGAACAAATCGAACTAAATCATTc                                                                       >  2:2858432/1‑91 (MQ=255)

                      tttttAGACTGCTCATATTCTTCAATCAACTTCGCTAATTTATTTTGATATTTGTACAACTGCTGTTGAACAAATCGAACTAAATCATTCg                                                                      <  2:2061402/91‑1 (MQ=255)

                      tttttAGACTGCTCATATTCTTCAATCAACTTCGCTAATTTATTTTGATATTTGTACAACTGCTGTTGAACAAATCGAACTAAATCATTCg                                                                      <  2:842500/91‑1 (MQ=255)

                       ttttAGACTGCTCATATTCTTCAATCAACTTCGCTAATTTATTTTGATATTTGTACAACTGCTGTTGAACAAATCGAACTAAATCATTCGc                                                                     >  2:1453356/1‑91 (MQ=255)

                       ttttAGACTGCTCATATTCTTCAATCAACTTCGCTAATTTATTTTGATATTTGTACAACTGCTGTTGAACAAATCGAACTAAATCATTCGc                                                                     >  1:1390064/1‑91 (MQ=255)

                        tttAGACTGCTCATATTCTTCAATCAACTTCGCTAATTTATTTTGATATTTGTACAACTGCTGTTGAACAAATCGAACTAAATCATTCGCa                                                                    >  1:3797933/1‑91 (MQ=255)

                        tttAGACTGCTCATATTCTTCAATCAACTTCGCTAATTTATTTTGATATTTGTACAACTGCTGTTGAACAAATCGAACTAAATCATTCGCa                                                                    <  1:3466227/91‑1 (MQ=255)

                          tAGACTGCTCATATTCTTCAATCAACTTCGCTAATTTATTTTGATATTTGTACAACTGCTGTTGAACAAATCGAACTAAATCATTCGCACg                                                                  <  2:1661031/91‑1 (MQ=255)

                          tAGACTGCTCATATTCTTCAATCAACTTCGCTAATTTATTTTGATATTTGTACAACTGCTGTTGAACAAATCGAACTAAATCATTCGCACg                                                                  <  2:2313676/91‑1 (MQ=255)

                           aGACTGCTCATATTCTTCAATCAACTTCGCTAATTTATTTTGATATTTGTACAACTGCTGTTGAACAAATCGAACTAAATCATTCGCACgt                                                                 >  2:12908/1‑91 (MQ=255)

                           aGACTGCTCATATTCTTCAATCAACTTCGCTAATTTATTTTGATATTTGTACAACTGCTGTTGAACAAATCGAACTAAATCATTCGCACgt                                                                 >  2:1039290/1‑91 (MQ=255)

                           aGACTGCTCATATTCTTCAATCAACTTCGCTAATTTATTTTGATATTTGTACAACTGCTGTTGAACAAATCGAACTAAATCATTCGCACgt                                                                 >  1:3345694/1‑91 (MQ=255)

                            gACTGCTCATATTCTTCAATCAACTTCGCTAATTTATTTTGATATTTGTACAACTGCTGTTGAACAAATCGAACTAAATCATTCGCACgtt                                                                <  1:1159561/91‑1 (MQ=255)

                               tGCTCATATTCTTCAATCAACTTCGCTAATTTATTTTGATATTTGTACAACTGCTGTTGAACAAATCGAACTAAATCATTCGCACgttgtt                                                             <  1:3085886/91‑1 (MQ=255)

                                gCTCATATTCTTCAATCAACTTCGCTAATTTATTTTGATATTTGTACAACTGCTGTTTAACAAATCGAACTAAATCATTCGCACGTTGttt                                                            >  2:1338072/1‑91 (MQ=255)

                                 cTCATATTCTTCAATCAACTTCGCTAATTTATTTTGATATTTGTACAACTGCTGTTGAACAAATCGAACTAAATCATTCGCACGTTGTTTa                                                           >  2:3277879/1‑91 (MQ=255)

                                      atTCTTCAATCAACTTCGCTAATTTATTTTGATATTTGTACAACTGCTGTTGAACAAATCGAACTAAATCATTCGCACGTTGTTTAAcgcg                                                      >  1:3207935/1‑91 (MQ=255)

                                      atTCTTCAATCAACTTCGCTAATTTATTTTGATATTTGTACAACTGCTGTTGAACAAATCGAACTAAATCATTCGCACGTTGTTTAAcgcg                                                      <  2:927852/91‑1 (MQ=255)

                                      atTCTTCAATCAACTTCGCTAATTTATTTTGATATTTGTACAACTGCTGTTGAACAAATCGAACTAAATCATTCGCACGTTGTTTAAcgcg                                                      >  1:3858709/1‑91 (MQ=255)

                                       ttcttcAATCAACTTCGCTAATTTATTTTGATATTTGTACAACTGCTGTTGAACAAATCGAACTAAATCATTCGCACGTTGTTTAACGCGt                                                     <  2:2669343/91‑1 (MQ=255)

                                       ttcttcAATCAACTTCGCTAATTTATCTTGATATTTGTACAACTGCTGTTGAACAAATCGAACTAAATCATTCGCACGTTGTTTAACGCGt                                                     >  2:2211878/1‑91 (MQ=255)

                                        tcttcAATCAACTTCGCTAATTTATTTTGATATTTGTACAACTGCTGTTGAACAAATCGAACTAAATCATTCGCACGTTGTTTAACGCGtt                                                    >  1:447437/1‑91 (MQ=255)

                                        tcttcAATCAACTTCGCTAATTTATTTTGATATTTGTACAACTGCTGTTGAACAAATCGAACTAAATCATTCGCACGTTGTTTAACGCGtt                                                    >  1:257354/1‑91 (MQ=255)

                                         cttcAATCAACTTCGCTAATTTATTTTGATATTTGTACAACTGCTGTTGAACAAATCGAACTAAATCATTCGCACGTTGTTTAACGCGTTc                                                   <  1:952231/91‑1 (MQ=255)

                                          ttcAATCAACTTCGCTAATTTATTTTGATATTTGTACAACTGCTGTTGAACAAATCGAACTAAATCATTCGCACGTTGTTTAACGCGTTCa                                                  >  2:2748567/1‑91 (MQ=255)

                                             aatcaaCTTCGCTAATTTATTTTGATATTTGTACAACTGCTGTTGAACAAATCGAACTAAATCATTCGCACGTTGTTTAACGCGTTCACGt                                               >  2:1952122/1‑91 (MQ=255)

                                             aatcaaCTTCGCTAATTTATTTTGATATTTGTACAACTGCTGTTGAACAAATCGAACTAAATCATTCGCACGTTGTTTAACGCGTTCACGt                                               >  2:137715/1‑91 (MQ=255)

                                               tcaaCTTCGCTAATTTATTTTGATATTTGTGCAGCTGCTGTTGAACAAATCGAACTAAATCATTCGCATGTTGTTTAACGCGTTCACGTTc                                             <  2:3425040/91‑1 (MQ=255)

                                                  aCTTCGCTAATTTATTTTGATATTTGTACAACTGCTGTTGAACAAATCGAACTAAATCATTCGCACGTTGTTTAACGCGTTCACGTTCGcc                                          <  1:1330222/91‑1 (MQ=255)

                                                   cTTCGCTAATTTATTTTGATATTTGTACAACTGCTGTTGAACAAATCGAACTAAATCATTCGCACGTTGTTTAACGCGTTCACGTTCGccc                                         >  1:875797/1‑90 (MQ=255)

                                                   cTTCGCTAATTTATTTTGATATTTGTACAACTGCTGTTGAACAAATCGAACTAAATCATTCGCACGTTGTTTAACGCGTTCACGTTCGCCa                                         <  2:897581/91‑1 (MQ=255)

                                                   cTTCGCTAATTTATTTTGATATTTGTACAACTGCTGTTGAACAAATCGAACTAAATCATTCGCACGTTGTTTAACGCGTTCACGTTCGCCa                                         <  2:369259/91‑1 (MQ=255)

                                                   cTTCGCTAATTTATTTTGATATTTGTACAACTGCTGTTGAACAAATCGAACTAAATCATTCGCACGTTGTTTAACGAGTTCACGTTCGTCa                                         >  1:77718/1‑91 (MQ=255)

                                                     tCGCTAATTTATTTTGATATTTGTACAACTGCTGTTGAACAAATCGAACTAAATCATTCGCACGTTGTTTAACGCGTTCACGTTCGCCAcg                                       >  2:257857/1‑91 (MQ=255)

                                                      cGCTAATTTATTTTGATATTTGTACAACTGCTGTTGAACAAATCGAACTAAATCATTCGCACGTTGTTTAACGCGTTCACGTTCGCCAcgc                                      >  2:1098357/1‑91 (MQ=255)

                                                      cGCTAATTTATTTTGATATTTGTACAACTGCTGTTGAACAAATCGAACTAAATCATTCGCACGTTGTTTAACGCGTTCACGTTCGCCAcgc                                      >  1:1524520/1‑91 (MQ=255)

                                                      cGCTAATTTATTTTGATATTTGTACAACTGCTGTTGAACAAATCGAACTAAATCATTCGCACGTTGTTTAACGCGTTCACGTTCGCCAcgc                                      >  1:2595480/1‑91 (MQ=255)

                                                      cGCTAATTTATTTTGATATTTGTACAACTGCTGTTGAACAAATCGAACTAAATCATTCGCACGTTGTTTAACGCGTTCACGTTCGCCAcgc                                      <  1:2139045/91‑1 (MQ=255)

                                                       gCTAATTTATTTTGATATTTGTACAACTGCTGTTGAACAAATCGAACTAAATCATTCGCACGTTGTTTAACGCGTTCACGTTCGCCAcgcg                                     >  2:448965/1‑91 (MQ=255)

                                                        cTAATTTATTTTGATATTTGTACAACTGCTGTTGAACAAATCGAACTAAATCATTCGCACGTTGTTTAACGCGTTCACGTTCGCCACgcgc                                    >  2:3480844/1‑91 (MQ=255)

                                                        cTAATTTATTTTGATATTTGTACAACTGCTGTTGAACAAATCGAACTAAATCATTCGCACGTTGTTTAACGCGTTCACGTTCGCCACgcgc                                    >  2:3558455/1‑91 (MQ=255)

                                                         tAATTTATTTTGATATTTGTACAACTGCTGTTGAACAAATCGAACTAAATCATTCGCACGTTGTTTAACGCGTTCACGTTCGCCACGCGca                                   >  2:3622855/1‑91 (MQ=255)

                                                          aaTTTATTTTGATATTTGTACAACTGCTGTTGAACAAATCGAACTAAATCATTCGCACGTTGTTTAACGCGTTCACGTTCGCCACGCGcat                                  <  1:2090486/91‑1 (MQ=255)

                                                           atttatttTGATATTTGTACAACTGCTGTTGAACAAATCGAACTAAATCATTCGCACGTTGTTTAACGCGTTCACGTTCGCCACGCGcatc                                 >  2:1430066/1‑91 (MQ=255)

                                                           atttatttTGATATTTGTACAACTGCTGTTGAACAAATCGAACTAAATCATTCGCACGTTGTTTAACGCGTTCACGTTCGCCACGCGcatc                                 >  1:3145926/1‑91 (MQ=255)

                                                                ttttGATATTTGTACAACTGCTGTTGAACAAATCGAACTAAATCATTCGCACGTTGTTTAACGCGTTCACGTTCGCCACGCGCATCATaaa                            >  2:3531033/1‑91 (MQ=255)

                                                                ttttGATATTTGTACAACTGCTGTTGAACAAATCGAACTAAATCATTCGCACGTTGTTTAACGCGTTCACGTTCGCCACGCGCATCATaaa                            <  1:2587173/91‑1 (MQ=255)

                                                                ttttGATATTTGTACAACTGCTGTTGAACAAATCGAACTAAATCATTCGCACGTTGTTTAACGCGTTCACGTTCGCCACGCGCATCATaaa                            <  1:1614319/91‑1 (MQ=255)

                                                                 tttGATATTTGTACAACTGCTGTTGAACAAATCGAACTAAATCATTCGCACGTTGTTTAACGCGTTCACGTTCGCCACGCGCATCATaaaa                           >  2:402569/1‑91 (MQ=255)

                                                                  ttGATATTTGTACAACTGCTGTTGAACAAATCGAACTAAATCATTCGCACGTTGTTTAACGCGTTCACGTTCGCCACGCGCATCATaaaaa                          >  2:3746888/1‑91 (MQ=255)

                                                                    gATATTTGTACAACTGCTGTTGAACAAATCGAACTAAATCATTCGCACGTTGTTTAACGCGTTCACGTTCGCCACGCGCCTCATAAAAACg                        >  2:172881/1‑91 (MQ=255)

                                                                        tttGTACAACTGCTGTTGAACAAATCGAACTAAATCATTCGCACGTTGTTTAACGCGTTCACGTTCGCCACGCGCATCATAAAAACGATCa                    <  2:3170058/91‑1 (MQ=255)

                                                                         ttGTACAACTGCTGTTGAACAAATCGAACTAAATCATTCGCACGTTGTTTAACGCGTTCACGTTCGCCACGCGCATCATAAAAACGATCaa                   <  1:225879/91‑1 (MQ=255)

                                                                         ttGTACAACTGCTGTTGAACAAATCGAACTAAATCATTCGCACGTTGTTTAACGCGTTCACGTTCGCCACGCGCATCATAAAAACGATCaa                   >  2:304365/1‑91 (MQ=255)

                                                                          tgttcAACAGCAGTTGTACAAATCGAACTAAATCATTCGCACGGTGTTTACCGCGTTCACGTTCGCCACGCGCATCCTAAAAACGCTCAAg                  >  2:1596980/5‑91 (MQ=255)

                                                                          tGTTCAACAGCAGTTGTACAAATCGAACTAAATCATTCGCACGTTGTTTAACGCGTTCACGTTCGCCACGCGCATCATAAAAACGATCAAg                  >  2:714499/1‑91 (MQ=255)

                                                                           gttcAACAGCAGTTGTACAAATCGAACTAAATCATTCGCACGTTGTTTAACGCGTTCACGTTCGCCACGCGCATCATAAAAACGATCAAGc                 >  1:486446/4‑91 (MQ=255)

                                                                           gTACAACTGCTGTTGAACAAATCGAACTAAATCATTCGCACGTTGTTTAACGCGTTCACGTTCGCCACGCGCATCATAAAAACGATCAAGc                 >  1:2809030/1‑91 (MQ=255)

                                                                           gTACAACTGCTGTTGAACAAATCGAACTAAACCATTCGCACGTTGTTTAACGCGTTCACGTTCGCCATGCGCATCATAATAACGATCAAGc                 >  2:2966118/1‑91 (MQ=255)

                                                                            tACAACTGCTGTTGAACAAATCGAACTAAATCATTCGCACGTTGTTTAACGCGTTCACGTTCGCCACGCGCATCATAAAAACGATCAAGCa                <  2:2127670/91‑1 (MQ=255)

                                                                             tcAACAGCAGTTGTACAAATCGAACTAAATCATTCGCCCGTTGTTTAACGCGTTCACGTTCGCCACGCGCCTCATAAAAACGCCCAAGCaa               >  2:54808/2‑91 (MQ=255)

                                                                              cAACTGCTGTTGAACAAATCGAACTAAATCATTCGCACGTTGTTTAACGCGTTCACGTTCGCCACGCGCATCATAAAAACGATCAAGCaaa              <  1:2191554/91‑1 (MQ=255)

                                                                              cAACTGCTGTTGAACAAATCGAACTAAATCATTCGCACGTTGTTTAACGCGTTCACGTTCGCCACGCGCATCATAAAAACGATCAAGCaaa              <  2:3590057/91‑1 (MQ=255)

                                                                                aCTGCTGTTGAACAAATCGAACTAAATCATTCGCACGTTGTTTAACGCGTTCACGTTCGCCACGCGCATCATAAAAACGATCAAGCAAATc            >  2:1743893/1‑91 (MQ=255)

                                                                                aCTGCTGTTGAACAAATCGAACTAAATCATTCGCACGTTGTTTAACGCGTTCACGTTCGCCACGCGCATCATAAAAACGATCAAGCAAATc            >  1:2642532/1‑91 (MQ=255)

                                                                                 ctgctgTTGAACAAATCGAACTAAATCATTCGCACGTTGTTTAACGCGTTCACGTTCGCCACGCGCATCATAAAAACGATCAAGCAAATCa           >  2:2778558/1‑91 (MQ=255)

                                                                                 ctgctgTTGAACAAATCGAACTAAATCATTCGCACGTTGTTTAACGCGTTCACGTTCGCCACGCGCATCATAAAAACGATCAAGCAAATCa           >  1:2405420/1‑91 (MQ=255)

                                                                                    cagTTGTACAAATCGAACTAAATCATTCGCACGTTGTTTAACGCGTTCACGTTCGCCACGCGCATCATAAAAACGATCAAGCAAATCAttt        >  2:153583/3‑91 (MQ=255)

                                                                                     tgTTGAACAAATCGAACTAAATCATTCGCACGTTGTTTAACGCGTTCACGTTCGCCACGCGCATCATAAAAACGATCAAGCAAATCATTTa       <  1:1502007/91‑1 (MQ=255)

                                                                                     tgTTGAACAAATCGAACTAAATCATTCGCACGTTGTTTAACGCGTTCACGTTCGCCACGCGCATCATAAAAACGATCAAGCAAATCATTTa       <  1:2281175/91‑1 (MQ=255)

                                                                                     tgTTGAACAAATCGAACTAAATCATTCGCACGTTGTTTAACGCGTTCACGTTCGCCACGCGCATCATAAAAACGATCAAGCAAATCATTTa       <  1:768377/91‑1 (MQ=255)

                                                                                     tgTTGAACAAATCGAACTAAATCATACGCACGTTGTTTAACGCGTTCACGTTCGCCACGCGCATCATAAAAACGATCAAGCAAATCATTTa       <  1:3607050/91‑1 (MQ=255)

                                                                                      gTTGTACAAATCGAACTAAATCATTCGCACGTTGTTTAACGCGTTCACGTTCGCCACGCGCATCATAAAAACGATCAAGCAAATCATTTaa      >  1:1833994/1‑91 (MQ=255)

                                                                                      gTTGAACAAATCGAACTAAATCATTCGCACGTTGTTTAACGCGTTCACGTTCGCCACGCGCATCATAAAAACGATCAAGCAAATCATTTaa      <  1:1450484/91‑1 (MQ=255)

                                                                                      gTTGAACAAATCGAACTAAATCATTCGCACGTTGTTTAACGCGTTCACGTTCGCCACGCGCATCATAAAAACGATCAAGCAAATCATTTaa      <  1:3248466/91‑1 (MQ=255)

                                                                                        tGAACAAATCGAACTAAATCATTCGCACGTTGTTTAACGCGTTCACGTTCGCCACGCGCATCATAAAAACGATCAAGCAAATCATTTAATg    >  2:2898429/1‑91 (MQ=255)

                                                                                         gAACAAATCGAACTAAATCATTCGCACGTTGTTTAACGCGTTCACGTTCGCCACGCGCATCATAAAAACGATCAAGCAAATCATTTAATGa   >  1:1003828/1‑91 (MQ=255)

                                                                                          aaCAAATCGAACTAAATCATTCGCACGTTGTTTAACGCGTTCACGTTCGCCACGCGCATCATAAAAACGATCAAGCAAATCATTTAATGaa  >  1:2348912/1‑91 (MQ=255)

ATATAACTGTTCAGTATCTTTATTTTTAGACTGCTCATATTCTTCAATCAACTTCGCTAATTTATTTTGATATTTGTACAACTGCTGTTGAACAAATCGAACTAAATCATTCGCACGTTGTTTAACGCGTTCACGTTCGCCACGCGCATCATAAAAACGATCAAGCAAATCATTTAATGAA  >  USA300TCH1516_ALE/1219489‑1219669

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`