| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 1,421,615 | C→G | 6.3% | intergenic (+51/+89) | pepF1_2 → / ← phoU | Oligoendopeptidase F, plasmid/Phosphate‑specific transport system accessory protein PhoU |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 1,421,615 | 0 | C | G | 6.3% | 814.2 / 28.6 | 232 | intergenic (+51/+89) | pepF1_2/phoU | Oligoendopeptidase F, plasmid/Phosphate‑specific transport system accessory protein PhoU |
| Reads supporting (aligned to +/- strand): ref base C (114/103); new base G (7/8); total (121/111) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.91e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 238 total aligned reads displayed.
TGAAAAATTAACAGATGAAATTGAGCAAGCAAATAACTAAGATGCGAGACAATTTATAGTAGAACAATATAAACCAAGATTTTCAC‑ATTACTAATTGTGAGAATCTTGGTTTTTTTGAGGGTTTATGAATAATAGAAATATATATTTATCGTAAGGGATTTTATAGTAATAACTTTAAGT > USA300TCH1516_ALE/1421525‑1421704 | tGAAAAATTAACAGATGAAATTGAGCAAGCAAATAACTAAGATGCGAGACAATTTATAGTAGAACAATATAAACCAAGATTTTCAC‑ATTAc < 1:2126890/91‑1 (MQ=255) gAAAAATTAACAGATGAAATTGAGCAAGCAAATAACTAAGATGCGAGACAATTTATAGTAGAACAATATAAACCAAGATTTTCAC‑ATTACt > 2:3676975/1‑91 (MQ=255) gAAAAATTAACAGATGAAATTGAGCAAGCAAATAACTAAGATGCGAGACAATTTATAGTAGAACAATATAAACCAAGATTTTCAC‑ATTACt > 1:3733308/1‑91 (MQ=255) gAAAAATTAACAGATGAAATTGAGCAAGCAAATAACTAAGATGCGAGACAATTTATAGTAGAACAATATAAACCAAGATTTTCAC‑ATTACt > 1:2252769/1‑91 (MQ=255) gAAAAATTAACAGATGAAATTGAGCAAGCAAATAACTAAGATGCGAGACAATTTATAGTAGAACAATATAAACCAAGATTTTCAC‑ATTACt > 2:3281496/1‑91 (MQ=255) gAAAAATTAACAGATGAAATTGAGCAAGCAAATAACTAAGATGCGAGACAATTTATAGTAGAACAATATAAACCAAGATTTTCAC‑ATTACt > 2:388111/1‑91 (MQ=255) aaaaaTTAACAGATGAAATTGAGCAAGCAAATAACTAAGATGCGAGACAATTTATAGTAGAACAATATAAACCAAGATTTTCAC‑ATTACTa < 2:3011920/91‑1 (MQ=255) aTTAACAGATGAAATTGAGCAAGCAAATAACTAAGATGCGAGACAATTTATAGTAGAACAATATAAACCAAGATTTTCAC‑ATTACTAATtg > 1:3435496/1‑91 (MQ=255) ttAACAGATGAAATTGAGCAAGCAAATAACTAAGATGCGAGACAATTTATAGTAGAACAATATAAACCAAGATTTTCAC‑ATTACTAATtgt < 1:1927127/91‑1 (MQ=255) tAACAGATGAAATTGAGCAAGCAAATAACTAAGATGCGAGACAATTTATAGTAGAACAATATAAACCAAGATTTTCAC‑ATTACTAATtgtg < 2:2038533/91‑1 (MQ=255) tAACAGATGAAATTGAGCAAGCAAATAACTAAGATGCGAGACAATTTATAGTAGAACAATATAAACCAAGATTTTCAC‑ATTACTAATtgtg < 2:3330544/91‑1 (MQ=255) tAACAGATGAAATTGAGCAAGCAAATAACTAAGATGCGAGACAATTTATAGTAGAACAATATAAACCAAGATTCTCACAATTAGTAAtgtg < 1:2615851/91‑4 (MQ=38) aaCAGATGAAATTGAGCAAGCAAATAACTAAGATGCGAGACAATTTATAGTAGAACAATATAAACCAAGATTTTCAC‑ATTACTAATTGTga > 1:3016502/1‑91 (MQ=255) aCAGATGAAATTGAGCAAGCAAATAACTAAGATGCGAGACAATTTATAGTAGAACAATATAAACCAAGATTTTCAC‑ATTACTAATTGTgag < 2:1313774/91‑1 (MQ=255) cAGATGAAATTGAGCAAGCAAATAACTAAGATGCGAGACAATTTATAGTAGAACAATATAAACCAAGATTTTCAC‑ATTACTAATTGTgaga > 1:1056877/1‑91 (MQ=255) gATGAAATTGAGCAAGCAAATAACTAAGATGCGAGACAATTTATAGTAGAACAATATAAACCAAGATTTTCAC‑ATTAc < 2:628239/78‑1 (MQ=255) aTGAAATTGAGCAAGCAAATAACTAGGATGCGAGACAATTTATAGTAGAACAATATAAACCAAGATTTTCAC‑ATTACTAATTGTGAGAATc < 1:3600656/91‑1 (MQ=255) tGAAATTGAGCAAGCAAATAACTAAGATGCGAGACAATTTATAGTAGAACAATATAAACCAAGATTTTCAC‑ATTACTAATTGTGAGAATCt < 2:1483849/91‑1 (MQ=255) tGAAATTGAGCAAGCAAATAACTAAGATGCGAGACAATTTATAGTAGAACAATATAAACCAAGATTTTCAC‑ATTACTAATTGTGAGAATCt > 2:2486104/1‑91 (MQ=255) tGAAATTGAGCAAGCAAATAACTAAGAGGCGAGACAATTTATAGTAGGACAATATAAACCAAGATTCTCACAATTAGTAA‑TGTGAAAATCt < 2:1112022/91‑1 (MQ=21) gAAATTGAGCAAGCAAATAACTAAGATGCGAGACAATTTATAGTAGAACAATATAAACCAAGATTTTCAC‑ATTACTAATTGTGAGAATCtt < 1:1852807/91‑1 (MQ=255) gAAATTGAGCAAGCAAATAACTAAGATGCGAGACAATTTATAGTAGAACAATATAAACCAAGATTTTCAC‑ATTACTAATTGTGAGAATCtt < 2:1206168/91‑1 (MQ=255) gAAATTGAGCAAGCAAATAACTAAGATGCGAGACAATTTATAGTAGAACAATATAAACCAAGATTCTCACAATTAGTAA‑TGTGAAAATCtt < 2:3791712/91‑1 (MQ=21) aTTGAGCCAGCAAATAACTAAGATGCGAGACAATTTATAGAAGAACACTATAAACCAAGATTTTCAA‑CTTACTACTAGTGAGAATCTTGGt > 2:3609771/1‑91 (MQ=255) aTTGAGCAAGCAAATAACTAAGATGCGAGACAATTTATAGTAGAACAATATAAACCAAGATTTTCAC‑ATTACTAATTGTGAGAATCTTGGt > 1:1585206/1‑91 (MQ=255) aTTGAGCAAGCAAATAACTAAGATGCGAGACAATTTATAGTAGAACAATATAAACCAAGATTTTCAC‑ATTACTAATTGTGAGAATCTTGGt > 1:1585694/1‑91 (MQ=255) aTTGAGCAAGCAAATAACTAAGATGCGAGACAATTTATAGTAGAACAATATAAACCAAGATTCTCACAATTAGTAA‑TGTGAAAATCTTGGt < 1:2001303/91‑1 (MQ=21) tGAGCAAGCAAATAACTAAGATGCGAGACAATTTATAGTAGAACAATATAAACCAAGATTTTCAC‑ATTACTAATTGTGAGAATCTTGGttt > 1:2708730/1‑91 (MQ=255) tGAGCAAGCAAATAACTAAGATGCGAGACAATTTATAGTAGAACAATATAAACCAAGATTTTCAC‑ATTACTAATTGTGAGAATCTTGGttt > 1:2915253/1‑91 (MQ=255) tGAGCAAGCAAATAACTAAGATGCGAGACAATTTATAGTAGAACAATATAAACCAAGATTCTCACAATTAGTAA‑TGTGAAAATCTTGGttt < 2:1230173/91‑1 (MQ=21) gAGCAAGCAAATAACTAAGATGCGAGACAATTTATAGTAGAACAATATAAACCAAGATTTTCAC‑ATTACTAATTGTGAGAATCTTGGtttt < 2:1358494/91‑1 (MQ=255) agcaagcaAATAACTAAGATGCGAGACAATTTATAGTAGAACAATATAAACCAAGATTTTCAC‑ATTACTAATTGTGAGAATCTTGGttttt > 1:2070522/1‑91 (MQ=255) gcaagcaaATAACTAAGATGCGAGACAATTTATAGTAGAACAATATAAACCAAGATTTTCAC‑ATTACTAATTGTGAGAATCTTGGtttttt < 2:742843/91‑1 (MQ=255) gcaagcaaATAACTAAGATGCGAGACAATTTATAGTAGAACAATATAAACCAAGATTTTCAC‑ATTACTAATTGTGAGAATCTTGGtttttt > 2:98825/1‑91 (MQ=255) gcaagcaaATAACTAAGATGCGAGACAATTTATAGTAGAACAATATAAACCAAGATTTTCAC‑ATTACTAATTGTGAGAATCTTGGtttttt < 1:2300568/91‑1 (MQ=255) caagcaaATAACTAAGATGCGAGACAATTTATAGTAGAACAATATAAACCAAGATTTTCAC‑ATTACTAATTGTGAGAATCTTGAttttttt > 2:3447006/1‑91 (MQ=255) aagcaaATAACTAAGATGCGAGACAATTTATAGTAGAACAATATAAACCAAGATTTTCAC‑ATTACTAATTGTGAGAATCTTGGTTTTTTTg < 2:1716847/91‑1 (MQ=255) aagcaaATAACTAAGATGCGAGACAATTTATAGTAGAACAATAGAAACCAAGATTTTCAC‑ATTACTAATTGTGAGAATCTTGGTTTTTTTg < 2:1685493/91‑1 (MQ=255) agcaaATAACTAAGATGCGAGACAATTTATAGTAGAACAATATAAACCAAGATTTTCAC‑ATTACTAATTGTGAGAATCTTGGTTTTTTTGa > 1:3413047/1‑91 (MQ=255) agcaaATAACTAAGATGCGAGACAATTTATAGTAGAACAATATAAACCAAGATTTTCAC‑ATTACTAATTGTGAGAATCTTGGTTTTTTTGa > 1:3538580/1‑91 (MQ=255) agcaaATAACTAAGATGCGAGACAATTTATAGTAGAACAATATAAACCAAGATTTTCAC‑ATTACTAATTGTGAGAATCTTGGTTTTTTTGa > 1:656038/1‑91 (MQ=255) agcaaATAACTAAGATGCGAGACAATTTATAGTAGAACAATATAAACCAAGATTTTCAC‑ATTACTAATTGTGAGAATCTTGGTTTTTTTGa > 1:606343/1‑91 (MQ=255) caaATAACTAAGATGCGAGACAATTTATAGTAGAACAATATAAACCAAGATTTTCAC‑ATTACTAATTGTGAGAATCTTGGTTTTTTTGAgg < 1:755986/91‑1 (MQ=255) aaTAACTAAGATGCGAGACAATTTATAGTAGAACAATATAAACCAAGATTTTCAC‑ATTACTAATTGTGAGAATCtt < 1:1475352/76‑1 (MQ=255) aCTAAGATGCGAGACAATTTATAGTAGAACAATATAAACCAAGATTTTCAC‑ATTACTAATTGTGAGAATCTTGGTTTTTTTGAGGGTTTAt < 2:2854131/91‑1 (MQ=255) aCTAAGATGCGAGACAATTTATAGTAGAACAATATAAACCAAGATTTTCAC‑ATTACTAATTGTGAGAATCTTGGTTTTTTTGAGGGTTTAt > 2:265745/1‑91 (MQ=255) aCTAAGATGCGAGACAATTTATAGTAGAACAATATAAACCAAGATTTTCAC‑ATTACTAATTGTGAGAATCTTGGTTTTTTTGAGGGTTTAt < 1:3018889/91‑1 (MQ=255) cTAAGATGCGAGACAATTTATAGTAGAACAATATAAACCAAGATTTTCAC‑ATTACTAATTGTGAGAATCTTGGTTTTTTTGAGGGTTTATg < 1:286575/91‑1 (MQ=255) cTAAGATGCGAGACAATTTATAGTAGAACAATATAAACCAAGATTTTCAC‑ATTACTAATTGTGAGAATCTTGGTTTTTTTGAGGGTTTATg > 2:1041243/1‑91 (MQ=255) cTAAGATGCGAGACAATTTATAGTAGAACAATATAAACCAAGATTTTCAC‑ATTACTAATTGTGAGAATCTTGGTTTTTTTGAGGGTTTATg > 2:1252698/1‑91 (MQ=255) aaGATGCGAGACAATTTATAGTAGAACAATATAAACCAAGATTTTCAC‑ATTACTAATTGTGAGAATCTTGGTTTTTTTGAGGGTTTATGaa < 2:7832/91‑1 (MQ=255) aaGATGCGAGACAATTTATAGTAGAACAATATAAACCAAGATTTTCAC‑ATTACTAATTGTGAGAATCTTGGTTTTTTTGAGGGTTTATGaa < 2:5679/91‑1 (MQ=255) aGATGCGAGACAATTTATAGTAGAACAATATAAACCAAGATTTTCAC‑ATTACTAATTGTGAGAATCTTGGTTTTTTTGAGGGTTTATGaat < 1:3236930/91‑1 (MQ=255) gATGCGAGACAATTTATAGTAGAACAATATAAACCAAGATTTTCAC‑ATTACTAATTGTGAGAATCTTGGTTTTTTTGAGGGTTTATGaata > 1:1399794/1‑91 (MQ=255) gCGAGACAATTTATAGTAGAACAATATAAACCAAGATTTTCAC‑ATTACTAATTGTGAGAATCTTGGTTTTTTTg < 1:3833080/74‑1 (MQ=255) gaCAATTTATAGTAGAACAATATAAACCAAGATTTTCAC‑ATTACTAATTGTGAGAATCTTGGTTTTTTTGAGGGTTTATGAATAATAGaaa > 1:3695457/1‑91 (MQ=255) gaCAATTTATAGTAGAACAATATAAACCAAGATTTTCAC‑ATTACTAATTGTGAGAATCTTGGTTTTTTTGAGGGTTTATGAATAATAGaaa > 1:2558381/1‑91 (MQ=255) aCAATTTATAGTAGAACAATATAAACCAAGATTTTCAC‑ATTACTAATTGTGAGAATCTTGGTTTTTTTGAGGGTTTATGAATAATAGAAat > 1:279414/1‑91 (MQ=255) aCAATTTATAGTAGAACAATATAAACCAAGATTTTCAC‑ATTACTAATTGTGAGAATCTTGGATTTTTTGAGGGTTTATGAATAATAGAAat > 1:3117928/1‑91 (MQ=255) cAATTTATAGTAGAACAATATAAACCAAGATTTTCAC‑ATTACTAATTGTGAGAATCTTGGTTTTTTTGAGGGTTTATGAATAATAGAAata > 1:2417407/1‑91 (MQ=255) aaTTTATAGTAGAACAGTATAAACCAAGATTTTCAC‑ATTACTAATTGTGAGAATCTTGGTTTTTTTGAGGGTTTATGAATAATAGAAatat < 2:3704203/91‑1 (MQ=255) aTTTATAGTAGAACAATATAAACCAAGATTTTCAC‑ATTACTAATTGTGAGAATCTTGGTTTTTTTGAGGGTTTATGAATAATAGAAatata < 1:444198/91‑1 (MQ=255) tttATAGTAGAACAATATAAACCAAGATTTTCAC‑ATTACTAATTGTGAGAATCTTGGTTTTTTTGAGGGTTTATGAATAATAGAAatatat < 1:968346/91‑1 (MQ=255) tttATAGTAGAACAATATAAACCAAGATTTTCAC‑ATTACTAATTGTGAGAATCTTGGTTTTTTTGAGGGTTTATGAATAATAGAAatatat < 1:3007994/91‑1 (MQ=255) tttATAGTAGAACAATATAAACCAAGATTTTCAC‑ATTACTAATTGTGAGAATCTTGGTTTTTTTGAGGGTTTATGAATAATAGAAatatat < 1:222365/91‑1 (MQ=255) ttATAGTAGAACAATATAAACCAAGATTTTCAC‑ATTACTAATTGTGAGAATCTTGGTTTTTTTGAGGGTTTATGAATAATAGAAatatata > 1:1999158/1‑91 (MQ=255) tataGTAGAACAATATAAACCAAGATTTTCAC‑ATTACTAATTGTGAGAATCTTGGTTTTTTTGAGGGTTTATGAATAATAGAAatatatat > 2:809640/1‑91 (MQ=255) ataGTAGAACAATATAAACCAAGATTTTCAC‑ATTACTAATTGTGAGAATCTTGGTTTTTTTGAGGGTTTATGAATAATAGAAATATATAtt < 2:1413989/91‑1 (MQ=255) tagtagAACAATATAAACCAAGATTTTCAC‑ATTACTAATTGTGAGAATCTTGGTTTTTTTGAGGGTTTATGAATAATAGAAATATATAttt > 2:2255983/1‑91 (MQ=255) tagAACAATATAAACCAAGATTTTCAC‑ATTACTAATTGTGAGAATCTTGGTTTTTTTGAGGGTTTATGAATAATAGAAATATATATTTATc > 1:3480152/1‑91 (MQ=255) tagAACAATATAAACCAAGATTTTCAC‑ATTACTAATTGTGAGAATCTTGGTTTTTTTGAGGGTTTATGAATAATAGAAATATATATTTATc > 1:1268465/1‑91 (MQ=255) tagAACAATATAAACCAAGATTTTCAC‑ATTACTAATTGTGAGAATCTTGGTTTTTTTGAGGGTTTATGAATAATAGAAATATATATTTATc > 1:1970473/1‑91 (MQ=255) gAACAATATAAACCAAGATTTTCAC‑ATTACTAATTGTGAGAATCTTGGTTTTTTTGAGGGTTTATGAATAATAGAAATATATATTTATCGt < 2:10569/91‑1 (MQ=255) aCAATATAAACCAAGATTTTCAC‑ATTACTAATTGTGAGAATCTTGGTTTTTTTGAGGGTTTATGAATAATAGAAATATATATTTATCGTaa < 2:2621117/91‑1 (MQ=255) aCAATATAAACCAAGATTTTCAC‑ATTACTAATTGTGAGAATCTTGGTTTTTTTGAGGGTTTATGAATAATAGAAATATATATTTATCGTaa < 1:3181104/91‑1 (MQ=255) cAATATAAACCAAGATTTTCAC‑ATTACTAATTGTGAGAATCTTGGTTTTTTTGAGGGTTTATGAATAATAGAAATATATATTTATCGTAAg < 2:1171572/91‑1 (MQ=255) aaTATAAACCAAGATTTTCAC‑ATTACTAATTGTGAGAATCTTGGTTTTTTTGAGGGTTTATGAATAATAGAAATATATATTTATCGTAAgg < 1:2244618/91‑1 (MQ=255) tataAACCAAGATTTTCAC‑ATTACTAATTGTGAGAATCTTGGTTTTTTTGAGGGTTTATGAATAATAGAAATATATATTTATCGTAAGGGa < 1:901788/91‑1 (MQ=255) tataAACCAAGATTTTCAC‑ATTACTAATTGTGAGAATCTTGGTTTTTTTGAGGGTTTATGAATAATAGAAATATATATTTATCGTAAGGGa < 2:3695457/91‑1 (MQ=255) aaaaCCAAGATTCTCACAATTAGTAA‑TGTGAAAATCTTGGTTTTTTTGAGGGTTTATGAATAATAGAAATATATATTTATCGTAAGGGAtt > 1:1367695/2‑91 (MQ=18) aaaCCAAGATTTTCAC‑ATTACTAATTGTGAGAATCTTGGTTTTTTTGAGGGTTTATGAATAATAGAAATATATATTTATCGTAAGGGAttt < 2:2177965/91‑1 (MQ=255) aaCCAAGATTTTCAC‑ATTACTAATTGTGAGAATCTTGGTTTTTTTGCGGGTTTATGAATAATAGAAATATATATTTATCGTAAGGGAtttt > 1:3091109/1‑91 (MQ=255) aaCCAAGATTTTCAC‑ATTACTAATTGTGAGAATCTTGGTTTTTTTGAGGGTTTATGAATAATAGAAATATATATTTATCGTAAGGGAtttt > 1:2511447/1‑91 (MQ=255) aaCCAAGATTTTCAC‑ATTACTAATTGTGAGAATCTTGGTTTTTTTGAGGGTTTATGAATAATAGAAATATATATTTATCGTAAGGGAtttt < 1:2560467/91‑1 (MQ=255) aaCCAAGATTCTCACAATTAGTAA‑TGTGAAAATCTTGGTTTTTTTGAGGGTTTATGAATAATAGAAATATATATTTATCGTAAGGGAtttt > 1:770046/1‑91 (MQ=21) aCCAAGATTTTCAC‑ATTACTAATTGTGAGAATCTTGGTTTTTTTGAGGGTTTATGAATAATAGAAATATATATTTATCGTAAGGGATTTta > 2:2087492/1‑91 (MQ=255) ccAAGATTTTCAC‑ATTACTAATTGTGAGAATCTTGGTTTTTTTGAGGGTTTATGAATAATAGAAATATATATTTATCGTAAGGGATTTtat > 1:2676259/1‑91 (MQ=255) ccAAGATTCTCACAATTAGTAA‑TGTGAAAATCTTGGTTTTTTTGAGGGTTTATGAATAATAGAAATATATATTTATCGTAAGGGATTTtat > 1:844317/1‑91 (MQ=21) aaGATTCTCACAATTAGTAA‑TGTGAAAATCTTGGTTTTTTTGAGGGTTTATGAATAATAGAAATATATATTTATCGTAAGGGATTTTATAg > 2:2392057/1‑91 (MQ=21) gATTTTCAC‑ATTACTAATTGTGAGAATCTTGGTTTTTTTGAGGGTTTATGAATAATAGAAATATATATTTATCGTAAGGGATTTTATAGta > 2:949956/1‑91 (MQ=255) aTTTTCAC‑ATTACTAATTGTGAGAATCTTGGTTTTTTTGAGGGTTTATGAATAATAGAAATATATATTTATCGTAAGGGATTTTATAGtaa > 2:2100352/1‑91 (MQ=255) ttttCAC‑ATTACTAATTGTGAGAATCTTGGTTTTTTTGAGGGTTTATGAATAATAGAAATATATATTTATCGTAAGGGATTTTATAGtaat < 2:1911140/91‑1 (MQ=255) ttCAC‑ATTACTAATTGTGAGAATCTTGGTTTTTTTGAGGGTTTATGAATAATAGAAATATATATTTATCGTAAGGGATTTTATAGtaataa > 1:1686565/1‑91 (MQ=255) cac‑aTTACTAATTGTGAGAATCTTGGTTTTTTTGAGGGTTTATGAATAATAGAAATATATATTTATCGTAAGGGATTTTATAGTAATAACt < 2:3518321/91‑1 (MQ=255) gc‑aTTACTAATTGTGAGAATCTTGGTTTTTTTGAGGATTTATGAATAATAGAAATATATATTTATCGTAAGGGATTTTATAGTAATAACtt < 2:3842895/90‑1 (MQ=255) c‑aTTACTAATTGTGAGAATCTTGGTTTTTTTGAGGGTTTATGAATAATAGAAATATATATTTATCGTAAGGGATTTTATAGTAATAACtta < 2:2201414/91‑2 (MQ=255) aTTACTAATTGTGAGAATCTTGGTTTTTTTGAGGGTTTATGAATAATAGAAATATATATTTATCGTAAGGGATTTTATAGTAATAACTTTa < 2:1509418/91‑1 (MQ=255) aTTACTAATTGTGAGAATCTGGGTTTTTTTGAGGGTTTATGAATAATAGAAATATATATTTATCGTAAGGGATTTTATAGTAATAACTTTa < 1:960717/91‑1 (MQ=255) aCTAATTGTGAGAATCTTGGTTTTTTTGAGGGTTTATGAATAATAGAAATATATATTTATCGTAAGGGATTTTATAGTAATAACTTTAAGt < 2:2977271/91‑1 (MQ=255) aCTAATTGTGAGAATCTTGGTTTTTTTGAGGGTTTATGAATAATAGAAATATATATTTATCGTAAGGGATTTTATAGTAATAACTTTAAGt < 1:1252698/91‑1 (MQ=255) | TGAAAAATTAACAGATGAAATTGAGCAAGCAAATAACTAAGATGCGAGACAATTTATAGTAGAACAATATAAACCAAGATTTTCAC‑ATTACTAATTGTGAGAATCTTGGTTTTTTTGAGGGTTTATGAATAATAGAAATATATATTTATCGTAAGGGATTTTATAGTAATAACTTTAAGT > USA300TCH1516_ALE/1421525‑1421704 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |