| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 1,447,040 | T→A | 6.0% | E65D (GAA→GAT) | cobT ← | Aerobic cobaltochelatase subunit CobT |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 1,447,040 | 0 | T | A | 6.0% | 792.2 / 28.9 | 233 | E65D (GAA→GAT) | cobT | Aerobic cobaltochelatase subunit CobT |
| Reads supporting (aligned to +/- strand): ref base T (110/108); new base A (3/11); total (114/119) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.12e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
Only 100 of 240 total aligned reads displayed.
TTTATCTTGTACTACTTCATTTACAATTTGTACATCCATCATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGATTATAGTAAGGGAATTTTTGGATTTTAACT > USA300TCH1516_ALE/1446950‑1447130 | tttATCTTGTACTACCTCATTTACAATTTGTACATCCATCATATGATAACCGTAAGCAACTAACATAACATCTGTTTTTAAACCAGCCAtt > 1:3309454/1‑91 (MQ=255) ttATCTTGTACTACTTCATTTACAATTTGTACATCCATCATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCAttt > 2:1647846/1‑91 (MQ=255) aTCTTGTACTACTTCATTTACAATTTGTACATCCATCATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCg > 2:3060685/1‑91 (MQ=255) tCTTGTACTACTTCATTTACAATTTGTACATCCATCATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTACACCAGCCATTTCGa > 2:1193443/1‑91 (MQ=255) cTTGTACTACTTCATTTACAATTTGTACATCCATCATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATATCGaa < 2:867323/91‑2 (MQ=255) cTTGTACTACTCCATTTACAATTTGTACATCCATCATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGat < 2:2593164/91‑1 (MQ=255) ttGTACTACTTCATTTACAATTTGTACATCCATCATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGata < 2:1834492/91‑1 (MQ=255) tactacTTCATTTACAATTTGTACATCCATCATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATgg < 1:3540031/91‑1 (MQ=255) actactTCATTTACAATTTGTACATCCATCATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGc < 1:111659/91‑1 (MQ=255) tactTCATTTACAATTTGTACATCCATCATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCtt < 2:1430392/91‑1 (MQ=255) tactTCATTTACAATTTGTACATCCATCATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCtt < 2:2613250/91‑1 (MQ=255) tactTCATTTACAATTTGTACATCCATCATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATATCGAAATGGCtg < 1:2453250/91‑2 (MQ=255) actTCATTTACAATTTGTACATCCATCATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTg > 2:229047/1‑91 (MQ=255) ctTCATTTACAATTTGTACATCCATCATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTgg < 1:405414/91‑1 (MQ=255) ttCATTTACAATTTGTACATCCATCATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGt < 2:204312/91‑1 (MQ=255) tCATTTACAATTTGTACATCCATCATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTc > 2:56112/1‑91 (MQ=255) tCATTTACAATTTGTACATCCATCATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATCTCGATATGGCTTGGTc > 2:2983091/1‑91 (MQ=255) cATTTACAATTTGTACATCCATCATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCg > 1:1133775/1‑91 (MQ=255) aTTTACAATTTGTACATCCATCATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTcac < 2:3720234/91‑3 (MQ=255) aTTTACAATTTGTACATCCATCATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGa < 1:3212613/91‑1 (MQ=255) tttACAATTTGTACATCCATCATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTcaca < 1:792741/91‑4 (MQ=255) tttACAATTTGTACATCCATCATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTcaca < 1:3882100/91‑4 (MQ=255) ttACAATTTGTACATCCATCATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGAtg > 1:281465/1‑91 (MQ=255) tACAATTTGTACATCCATCATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGAtgt < 2:1414782/91‑1 (MQ=255) cAATTTGTACATCCATCATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGa > 2:3694684/1‑91 (MQ=255) cAATTTGTACATCCATCATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGa > 2:493081/1‑91 (MQ=255) aTTTGTACATCCATCATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGAcc > 2:1130185/1‑91 (MQ=255) aTTTGTACATCCATCATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGAcc < 1:306704/91‑1 (MQ=255) ttGTACATCCATCATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCaa < 2:2097129/91‑1 (MQ=255) tGTACATCCATCATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCaaa > 1:417400/1‑91 (MQ=255) tGTACATCCATCATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCaaa > 2:3547475/1‑91 (MQ=255) tGTACATCCATCATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCaaa < 1:2006996/91‑1 (MQ=255) gTACATCCATCATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCaaaa < 1:1607183/91‑1 (MQ=255) gTACATCCATCATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCaaaa < 1:3666518/91‑1 (MQ=255) catccatcATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCa < 1:2983279/91‑1 (MQ=255) catccatcATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCa > 2:2105587/1‑91 (MQ=255) atccatcATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCaa < 2:1826458/91‑1 (MQ=255) tccatcATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCa > 1:2261339/1‑83 (MQ=255) catcatATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTc < 2:1726615/91‑1 (MQ=255) atcatATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCg < 1:1710477/91‑1 (MQ=255) tatGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAAt > 1:379102/1‑91 (MQ=255) tatGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAAt < 1:542750/91‑1 (MQ=255) atGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATc > 2:3782253/1‑91 (MQ=255) tGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCa < 1:3846727/91‑1 (MQ=255) tGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGCAATCa > 2:3147488/1‑91 (MQ=255) gATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCaa > 2:670669/1‑91 (MQ=255) gATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCaa > 2:1081522/1‑91 (MQ=255) aTAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCaaa > 2:3373424/1‑91 (MQ=255) aTAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCaaa > 2:1231390/1‑91 (MQ=255) aaCCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAAc > 2:2065192/1‑91 (MQ=255) ccGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACAt < 2:1957872/91‑1 (MQ=255) ccGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACAt < 1:1102295/91‑1 (MQ=255) gTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACAttt > 1:1226254/1‑91 (MQ=255) gTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACAttt < 1:1454780/91‑1 (MQ=255) aaGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTg < 1:194824/91‑1 (MQ=255) agcagcTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGa > 2:2820252/1‑91 (MQ=255) gcTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAg > 1:2059779/1‑91 (MQ=255) gcTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAg > 1:725768/1‑91 (MQ=255) gcTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAg < 2:2417438/91‑1 (MQ=255) gcTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAg > 2:1985115/1‑91 (MQ=255) cTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAgg > 1:1411612/1‑91 (MQ=255) taacatatcaTCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGa < 1:2445567/91‑1 (MQ=255) taacataacaTCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGa > 1:1640321/1‑91 (MQ=255) taacataacaTCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGa < 1:1923562/91‑1 (MQ=255) taacataacaTCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGa < 2:3282024/91‑1 (MQ=255) ataacatCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACAtt < 1:1556513/77‑1 (MQ=255) ataacatCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGATtat > 2:5354/1‑91 (MQ=255) taacatCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGATtata > 1:2116223/1‑91 (MQ=255) taacatCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGATtata > 2:240648/1‑91 (MQ=255) aacatCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGATTATAg > 1:2502694/1‑91 (MQ=255) acatCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGATTATAGt > 1:1799145/1‑91 (MQ=255) tCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGATTATAGTAAg < 2:2746333/91‑1 (MQ=255) tCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGATTATAGTAAg < 1:766471/91‑1 (MQ=255) cTGTTTTTAAACCAGCCATTTCGATATTGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGATTATAGTAAgg < 2:1618278/91‑1 (MQ=255) cTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGATTATAGTAAgg < 1:3172571/91‑1 (MQ=255) cTGTTTTTAAACCAGCCATTTCGATATGGCCTGGTCGATGTGACCAAAACCAACTCGTAATCAACACATTTTGAACAGGATTATAGTAAgg > 2:387210/1‑91 (MQ=255) tGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGATTATAGTAAggg > 1:1336006/1‑91 (MQ=255) gTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGATTATAGTAAGGGa > 2:3309244/1‑91 (MQ=255) gTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGATTATAGTAAGGGa < 1:3511858/91‑1 (MQ=255) tttttAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGATTATAGTAAGGGaa > 2:582712/1‑91 (MQ=255) tttttAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGATTATAGTAAGGGaa < 2:3507933/91‑1 (MQ=255) ttttAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGATTATAGTAAGGGAAt < 1:837500/91‑1 (MQ=255) tttAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGATTATAGTAAGGGAAtt < 2:3566007/91‑1 (MQ=255) aaCCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGATTATAGTAAGGGAATTTTTg < 2:2822133/91‑1 (MQ=255) aaCCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGATTATAGTAAGGGAATTTTTg < 2:46060/91‑1 (MQ=255) ccaaGCCATATCGAAATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGATTATAGTAAGGGAATTTTTgg > 1:935706/4‑91 (MQ=255) aCCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGATTATAGTAAGGGAATTTTTgg > 1:2747957/1‑91 (MQ=255) ccAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGATTATAGTAAGGGAATTTTTGGa > 2:3168851/1‑91 (MQ=255) cAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAAGCAACTCGTAATCAAAACATTTTGAACAGGATTATAGTAAGGGAATTTCTGGAt > 1:3612747/1‑91 (MQ=255) cAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGATTATAGTAAGGGAATTTTTGGAt > 1:886723/1‑91 (MQ=255) aGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGATTATAGTAAGGGAATTTTTGGAtt > 1:2871244/1‑91 (MQ=255) gCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGATTATAGTAAGGGAATTTTTGGAttt > 2:1460341/1‑91 (MQ=255) gCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGATTATAGTAAGGGAATTTTTGGAttt > 1:3390497/1‑91 (MQ=255) ccATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGATTATAGTAAGGGAATTTTTGGAtttt < 2:3788541/91‑1 (MQ=255) cATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGATTATAGTAAGGGAATTTTTGGATTTTa > 1:2745425/1‑91 (MQ=255) cATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGATTATAGTAAGGGAATTTTTGGATTTTa > 1:1235501/1‑91 (MQ=255) cATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGATTATAGTAAGGGAATTTTTGGATTTTa > 2:2206841/1‑91 (MQ=255) aTTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGATTATAGTAAGGGAATTTTTGGATTTTaa < 2:1513740/91‑1 (MQ=255) tttCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGATTATAGTAAGGGAATTTTTGGATTTTAAc < 1:2215066/91‑1 (MQ=255) ttCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGATTATAGTAAGGGAATTTTTGCATTTTAACt > 1:3720047/1‑91 (MQ=255) | TTTATCTTGTACTACTTCATTTACAATTTGTACATCCATCATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGATTATAGTAAGGGAATTTTTGGATTTTAACT > USA300TCH1516_ALE/1446950‑1447130 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |