| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 1,845,295 | A→G | 8.1% | D733D (GAT→GAC) | isdH ← | Iron‑regulated surface determinant protein H |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 1,845,295 | 0 | A | G | 8.1% | 588.1 / 31.5 | 197 | D733D (GAT→GAC) | isdH | Iron‑regulated surface determinant protein H |
| Reads supporting (aligned to +/- strand): ref base A (101/80); new base G (3/13); total (104/93) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.21e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.02e-01 | |||||||||||
Only 100 of 214 total aligned reads displayed.
ATCTTTATCCACATTTCTATCAGTATCTTTGGCAATTTGAGTATCCATTTCTTTTAAATCATATTTATCGAAGTGATTA‑TTATCCGACATATCGGATAAATGATCAACATCATGTTGCACATCTTTATCAATATTATTATCAGCATCTTTAACCACGTCAGACTCTGGTTCTATCACATCT > USA300TCH1516_ALE/1845205‑1845385 | aTCTTTATCCACTTTTCTATCAGTATCTTTGGCAATTTGAGTATCCATTTCTTTTAGATCATATTTATCGAAGTGATTA‑TTATCCGACata < 2:1078889/91‑1 (MQ=255)aTCTTTATCCACATTTCTATCAGTATCTTTGGCAATTTGAGTATCCATTTCTTTTAAATCATATTTATCGAAGTGATTA‑TTATCCGACata < 2:2897180/91‑1 (MQ=255) tttATCCACATTTCTATCAGTATCTTTGGCAATTTGAGTATCCATTTCTTTTAAATCATATTTATCGAAGTGATTA‑TTATCCGACATATCg > 1:2928681/1‑91 (MQ=255) ttATCCACATTTCTATCAGTATCTTTGGCAATTTGAGTATCGATTTCTTTTAAATCATATTTATCGAAGTGATTA‑TTATCCGACATATCgg < 2:3412925/91‑1 (MQ=255) ttATCCACATTTCTATCAGTATCTTTGGCAATTTGAGTATCCATTTCTTTTAAATCATATTTATCGAAGTGATTA‑TTATCCGATATGTCgg < 1:2992126/91‑1 (MQ=255) ttATCCACATTTCTATCAGTATCTTTGGCAATTTGAGTATCCATTTCTTTTAAATCATATTTATCGAAGTGATTA‑TTATCCGACATATCgg > 1:1144377/1‑91 (MQ=255) tATCCACATTTCTATCAGTATCTTTGGCAATTTGAGTATCCATTTCTTTTAAATCATATTTATCGAAGTGATTA‑TTATCCGACATATCGGa < 2:851480/91‑1 (MQ=255) aTCCACATTTCTATCAGTATCTTTGGCAATTTGAGTATCCATTTCTTTTAAATCATATTTATCGAAGTGATTA‑TTATCCGATATGTCGGAt < 2:95060/91‑1 (MQ=255) aTCCACATTTCTATCAGTATCTTTGGCAATTTGAGTATCCATTTCTTTTAAATCATATTTATCGAAGTGATTA‑TTATCCGACATATCGGAt > 1:1628329/1‑91 (MQ=255) aTCCACATTTCTATCAGTATCTTTGGCAATTTGAGTATCCATTTCTTTTAAATCATATTTATCGAAGTGATTA‑TTATCCGACATATCGGAt > 1:3227335/1‑91 (MQ=255) tCCACATTTCTATCAGTATCTTTGGCAATTTGAGTATCCATTTCTTTTAAATCATATTTATCGAAGTGATTA‑TTATCCGATATGTCGGATa < 2:2136528/91‑1 (MQ=255) tCCACATTTCTATCAGTATCTTTGGCAATTTGAGTATCCATTTCTTTTAAATCATATTTATCGAAGTGATTA‑TTATCCGACATATCGGATa < 2:2859144/91‑1 (MQ=255) ccACATTTCTATCAGTATCTTTGGCAATTTGAGTATCCATTTCTTTTAAATCATATTTATCGAAGTGATTA‑TTATCCGATATGTCGGATaa < 1:3701737/91‑1 (MQ=255) ccACATTTCTATCAGTATCTTTGGCAATTTGAGTATCCATTTCTTTTAAATCATATTTATCGAAGTGATTA‑TTATCCGATATGTCGGATaa < 1:182914/91‑1 (MQ=255) cacaTTTCTATCAGTATCTTTGGCAATTTGAGTATCCATTTCTTTTAAATCATATTTATCGAAGTGATTA‑TTATCCGATATGTCGGATaat < 1:3138712/91‑2 (MQ=255) cacaTTTCTATCAGTATCTTTGGCAATTTGAGTATCCATTTCTTTTAAATCATATTTATCGAAGTGATTA‑TTATCCGATATGTCGGATaat < 1:703909/91‑2 (MQ=255) cacaTTTCTATCAGTATCTTTGGCAATTTGAGTATCCATTTCTTTTAAATCATATTTATCGAAGTGATCATTTATCCGATATGTCGGATaa < 1:1497401/91‑1 (MQ=37) aTTTCTATCAGTATCTTTGGCAATTTGAGTATCCATTTCTTTTAAATCATATTTATCGAAGTGATTA‑TTATCCGACATATCGGATAAATGa > 2:3114786/1‑91 (MQ=255) aTTTCTATCAGTATCTTTGGCAATTTGAGTAACCATTTCTTTTAAATCATATTTATCGAAGTGATTA‑TTATCCGACATATCGGATAAATGa > 1:3211208/1‑91 (MQ=255) ttCTATCAGTATCTTTGGCAATTTGAGTATCCATTTCTTTTAAATCATATTTATCGAAGTGATTA‑TTATCCGACATATCGGATAAATGATc < 1:2250012/91‑1 (MQ=255) tCTATCAGTATCTTTGGCAATTTGAGTATCCATTTCTTTTAAATCATATTTATCGAAGTGATTA‑TTATCCGACATATCGGATAAATGATCa > 1:847552/1‑91 (MQ=255) tCTATCAGTATCTTTGGCAATTTGAGTATCCATTTCTTTTAAATCATATTTATCGAAGTGATTA‑TTATCCGACATATCGGATAAATGATCa < 1:1430972/91‑1 (MQ=255) cTATCAGTATCTTTGGCAATTTGAGTATCCATTTCTTTTAAATCATATTTATCGAGGTGATTA‑TTATCCGACATATCGGATAAATGATCaa < 2:1902458/91‑1 (MQ=255) cTATCAGTATCTTTGGCAATTTGAGTATCCATTTCTTTTAAATCATATTTATCGAAGTGATTA‑TTATCCGACATATCGGATAAATGATCaa > 1:2978940/1‑91 (MQ=255) tATCAGTATCTTTGGCAATTTGAGTATCCATTTCTTTTAAATCATATTTATCGAAGTGATTA‑TTATCCGACATATCGGATAAATGATCAAc < 2:3670635/91‑1 (MQ=255) aTCAGTATCTTTGGCAATTTGAGTATCCATTTCTTTTAAATCATATTTATCGAAGTGATTA‑TTATCCGACATATCGGATAAATGATCAAca > 2:199130/1‑91 (MQ=255) aTCAGTATCTTTGGCAATTTGAGTATCCATTTCTTTTAAATCATATTTATCGAAGTGATTA‑TTATCCGACATATCGGATAAATGATCAAca < 2:3152447/91‑1 (MQ=255) tCAGTATCTTTGGCAATTTTAGTATCCATTTCTTTTAAATCATATTTATCGAAGTGATTA‑TTATCCGACATATCGGATAAATGATCAAcat < 2:826154/91‑1 (MQ=255) tCAGTATCTTTGGCAATTTGAGTATCCATTTCTTTTAAATCATATTTATCGAAGTGATTA‑TTATCCGACATATCGGATAAATGATCAAcat > 2:1487614/1‑91 (MQ=255) tCAGTATCTTTGGCAATTTGAGTATCCATTTCTTTTAAATCATATTTATCGAAGTGATTA‑TTATCCGACATATCGGATAAATGATCAAcat > 1:3168640/1‑91 (MQ=255) cAGTATCTTTGGCAATTTGAGTATCCATTTCTTTTAAATCATATTTATCGAAGTGATTA‑TTATCCGACATATCGGATAAATGATCAAcatc < 1:1719556/91‑1 (MQ=255) aGTATCTTTGGCAATTTGAGTATCCATTTCTTTTAAATCATATTTATCGAAGTGATTA‑TTATCCGACATATCGGATAAATGATCAAcatca < 1:916808/91‑1 (MQ=255) tATCTTTGGCAATTTGAGTATCCATTTCTTTTAAATCATATTTATCGAAGTGATTA‑TTATCCGACATATCGGATAAATGATCAACATCATg < 2:158818/91‑1 (MQ=255) aTCTTTGGCAATTTGAGTATCCATTTCTTTTAAATCATATTTATCGAAGTGATTA‑TTATCCGACATATCGGATAAATGATCAACATCATGt < 2:573917/91‑1 (MQ=255) aTCTTTGGCAATTAGAGTATCCATTTCTTTTAAATCATATTTAGCGAAGTGATTA‑TTATCCGACATATCGGATAAATGATCAACATCATGt < 1:3882201/91‑1 (MQ=255) tCTTTGGCAATTTGAGTATCCATTTCTTTTAAATCATATTTATTGAAGTGATTA‑TTATCCGACATATCGGATAAATGATCAACATCATGtt < 2:2442302/91‑1 (MQ=255) tCTTTGGCAATTTGAGTATCCATTTCTTTTAAATCATATTTATCGAAGTGATTA‑TTATCCGACATATCGGATAAATGATCAACATCATGtt > 1:2188720/1‑91 (MQ=255) cTTTGGCAATTTGAGTATCCATTTCTTTTAAATCATATTTATCGAAGTGATTA‑TTATCCGACATATCGGATAAATGATCAACATCATGTTg > 2:2120520/1‑91 (MQ=255) tttGGCAATTTGAGTATCCATTTCTTTTAAATCATATTTATCGAAGTGATTA‑TTATCCGACATATCGGATAAATGATCAACATCATGTTGc > 2:448956/1‑91 (MQ=255) ttGGCAATTTGAGTATCCATTTCTTTTAAATCATATTTATCGAAGTGATTA‑TTATCCGACATATCGGATAAATGATCAACATCATGTTGca < 1:2835480/91‑1 (MQ=255) gCAATTTGAGTATCCATTTCTTTTAAATCATATTTATCGAAGTGATTA‑TTATCCGACATATCGGATAAATGATCAACATCATGTTGCACAt < 1:1644238/91‑1 (MQ=255) aTTTGAGTATCCATTTCTTTTAAATCATATTTATCGAAGTGATTA‑TTATCCGACATATCGGATAAATGATCAACATCATGTTGCACATCtt > 2:256578/1‑91 (MQ=255) tttGAGTATCCATTTCTTTTAAATCATATTTATCGAAGTGATTA‑TTATCCGACATATCGGATAAATGATCAACATCATGTTGCACATCttt > 1:2238195/1‑91 (MQ=255) tttGAGTATCCATTTCTTTTAAATCATATTTATCGAAGTGATTA‑TTATCCGACATATCGGATAAATGATCAACATCATGTTGCACATCttt < 2:3174971/91‑1 (MQ=255) ttGAGTATCCATTTCTTTTAAATCATATTTATCGAAGTGATTA‑TTATCCGACATATCGGATAAATGATCAACATCATGTTGCACATCTTTa < 2:3694505/91‑1 (MQ=255) ttGAGTATCCATTTCTTTTAAATCATATTTATCGAAGTGATTA‑TTATCCGACATATCGGATAAATGATCAACATCATGTTGCACATCTTTa < 2:2910725/91‑1 (MQ=255) tGAGTATCCATTTCTTTTAAATCATATTTATCGAAGTGATTA‑TTATCCGACATATCGGATAAATGATCAACATCATGTTGCACATCTTTAt > 2:1400266/1‑91 (MQ=255) aGTATCCATTTCTTTTAAATCATATTTATCGAAGTGATTA‑TTATCCGACATATCGGATAAATGATCAACATCATGTTGCACATCTTTATCa > 1:2669484/1‑91 (MQ=255) gTATCCATTTCTTTTAAATCATATTTATCGAAGTGATTA‑TTCTCCGACATATCGGATAAATGATCAACATCATGTTGCACATCTTTATCaa > 2:2199418/1‑91 (MQ=255) ccATTTCTTTTAAATCATATTTATCGAAGTGATTA‑TTATCCGACATATCGGATAAATGATCAACATCATGTTGCACATCTTTATCAAtatt < 2:3284827/91‑1 (MQ=255) cATTTCTTTTAAATCATATTTATCGAAGTGATTA‑TTATCCGACATATCGGATAAATGATCAACATCATGTTGCACATCTTTATCAAtatta > 1:1384452/1‑91 (MQ=255) aTTTCTTTTAAATCATATTTATCGAAGTGATTA‑TTATCCGACATATCGGATAAATGATCAACATCATGTTGCACATCTTTATCAAtattat < 1:1024338/91‑1 (MQ=255) tttCTTTTAAATCATATTTATCGAAGTGATTA‑TTATCCGACATATCGGATAAATGATCAACATCATGTTGCACATCTTTATCAAtattatt > 1:861143/1‑91 (MQ=255) tttCTTTTAAATCATATTTATCGAAGTGATTA‑TTATCCGACATATCGGATAAATGATCAACATCATGTTGCACATCTTTATCAAtattatt > 1:1282590/1‑91 (MQ=255) ttCTTTTAAATCATATTTATCGAAGTGATTA‑TTATCCGACATATCGGATAAATGATCAACATCATGTTGCACATCTTTATCAAtattatta > 1:3690109/1‑91 (MQ=255) ttCTTTTAAATCATATTTATCGAAGTGATTA‑TTATCCGACATATCGGATAAATGATCAACATCATGTTGCACATCTTTATCAAtattatta > 2:807191/1‑91 (MQ=255) ttCTTTTAAATCATATTTATCGAAGTGATTA‑TTATCCGACATATCGGATAAATGATCAACATCATGTTGCACATCTTTATCAAtattatta < 1:676978/91‑1 (MQ=255) cTTTTAAATCATATTTATCGAAGTGATTA‑TTATCCGACATATCGGATAAATGATCAACATCATGTTGCACATCTTTATCAATATTATTATc < 1:346067/91‑1 (MQ=255) tttAAATCATATTTATCGAAGTGATTA‑TTATCCGACATATCGGATAAATGATCAACATCATGTTGCACATCTTTATCAATATTATTATCat > 1:1867544/1‑90 (MQ=255) tttAAATCATATTTATCGAAGTGATTA‑TTATCCGACATATCGGATAAATGATCAACATCATGTTGCACATCTTTATCAATATTATTATCAg > 2:166497/1‑91 (MQ=255) tttAAATCATATTTATCGAAGTGATTA‑TTATCCGACATATCGGATAAATGATCAACATCATGTTGCACATCTTTATCAATATTATTATCAg > 2:2763554/1‑91 (MQ=255) tttAAATCATATTTATCGAAGTGATTA‑TTATCCGACATATCGGATAAATGATCAACATCATGTTGCACATCTTTATCAATATTATTATCAg < 1:2192500/91‑1 (MQ=255) tttAAATCATATTTATCGAAGTGATTA‑TTATCCGACATATCGGATAAATGATCAACATCATGTTGCACATCTTTATCAATATTATTATCAg > 2:3387936/1‑91 (MQ=255) tAAATCATATTTATCGAAGTGATTA‑TTATCCGACATATCGGATAAATGATCAACATCATGTTGCACATCTTTATCAATATTATTATCAGCa > 1:883267/1‑91 (MQ=255) aaTCATATTTATCGAAGTGATTA‑TTATCCGACATATCGGATAAATGATCAACATCATGTTGCACATCTTTATCAATATTATTATCAGCATc < 1:971581/91‑1 (MQ=255) cATATTTATCGAAGTGATTA‑TTATCCGACATATCGGATAAATGATCAACATCATGTTGCACATCTTTATCAATATTATTATCAGCATCttt < 2:2040816/91‑1 (MQ=255) atatTTATCGAAGTGATTA‑TTATCCGACATATCGGATAAATGATCAACATCATGTTGCACATCTTTATCAATATTATTATCAGCATCTTTa > 2:3131925/1‑91 (MQ=255) tatTTATCGAAGTGATTA‑TTATCCGACATATCGGATAAATGATCAACATCATGTTGCACATCTTTATCAATATTATTATCAGCATCTTTaa > 1:645503/1‑91 (MQ=255) tttATCGAAGTGATTA‑TTATCCGACATATCGGATAAATGATCAACATCATGTTGCACATCTTTATCAATATTATTATCAGCATCTTTAAcc < 2:747121/91‑1 (MQ=255) tttATCGAAGTGATTA‑TTATCCGACATATCGGATAAATGATCAACATCATGTTGCACATCTTTATCAATATTATTATCAGCATCTTTAAcc > 1:3104450/1‑91 (MQ=255) tATCGAAGTGATTA‑TTATCCGACATATCGGATAAATGATCAACATCATGTTGCACATCTTTATCAATATTATTATCAGCATCTTTAACCAc > 2:48457/1‑91 (MQ=255) tATCGAAGTGATTA‑TTATCCGACATATCGGATAAATGATCAACATCATGTTGCACATCTTTATCAATATTATTATCAGCATCTTTAACCAc < 2:2651400/91‑1 (MQ=255) tCGAAGTGATTA‑TTATCCGACATATCGGATAAATGATCAACATCATGTTGCACATCTTTATCAATATTATTATCAGCATCTTTAACCACGt > 2:230813/1‑91 (MQ=255) cGAAGTGATTA‑TTATCCGACATATCGGATAAATGATCAACATCATGTTGCACATCTTTATCAATATTATTATCAGCATCTTTAACCACGTc < 1:2150372/91‑1 (MQ=255) aaGTGATTA‑TTATCCGACATATCGGATAAATGATCAACATCATGTTGCACATCTTTATCAATATTATTATCAGCATCTTTAACCACGTCAg < 1:1171933/91‑1 (MQ=255) aGTGATTA‑TTATCCGACATATCGGATAAATGATCAACATCATGTTGCACATCTTTATCAATATTATTATCAGCATCTTTAACCACGTCAGa > 1:1333400/1‑91 (MQ=255) tGATTA‑TTATCCGACATATCGGATAAATGATCAACATCATGTTGCACATCTTTATCAATATTATTATCAGCATCTTTAACCACGTCAGAct < 2:676709/91‑1 (MQ=255) gATTA‑TTATCCGACATATCGGATAAATGATCAACATCATGTTGCACATCTTTATCAATATTATTGTCAGCATCTTTAACCACGTCAGActc < 2:2215073/91‑1 (MQ=255) gATTA‑TTATCCGACATATCGGATAAATGATCAACATCATGTTGCACATCTTTATCAATATTATTATCAGCATCTTTAACCACGTCAGActc < 1:384839/91‑1 (MQ=255) tta‑ttaTCCGACATATCGGATAAATGATCAACATCATGTTGCACATCTTTATCAATATTATTATCAGCATCTTTAACCACGTCAGACTCTg > 1:697752/1‑91 (MQ=255) ta‑ttatCCGACATATCGGATAAATGATCAACATCATGTTGCACATCTTTATCAATATTATTATCAGCATCTTTAACCACGTCAGACTCTgg < 1:330050/91‑1 (MQ=255) a‑ttatCCGACATATCGGATAAATGATCAACATCATGTTGCACATCTTTATCAATATTATTATCAGCATCTTTAACCACGTCAGACTCTGGt > 1:724369/1‑91 (MQ=255) ttatCCGACATATCGGATAAATGATCAACATCATGTTGCACATCTTTATCAATATTATTATCAGCATCTTTAACCACGTCAGACTCTGGtt < 2:1824977/91‑1 (MQ=255) tatCCGACATATCGGATAAATGATCAACATCATGTTGCACATCTTTATCAATATTATTATCAGCATCTTTAACCACGTCAGACTCTGGTTc < 1:2120520/91‑1 (MQ=255) atCCGACATATCGGATAAATGATCACCATCATGTTGCACATCTTTATCAATATTATTATCAGCATCTTTAACCACGTCAGACTCTGGTTCt < 1:3609793/91‑1 (MQ=255) cGACATATCGGATAAATGATCAACATCATGTTGCACATCTTTATCAATATTATTATCAGCATCTTTAACCACGTCAGACTCTGGTTCTATc < 1:2732964/91‑1 (MQ=255) cGACATATCGGATAAATGATCAACATCATGTTGCACATCTTTATCAATATTATTATCAGCATCTTTAACCACGTCAGACTCTGGTTCTATc > 1:566343/1‑91 (MQ=255) cGACATATCGGATAAATGATCAACATCATGTTGCACATCTTTATCAATATTATTATCAGCATCTTTAACCACGTCAGACTCCGGTTCTATc > 1:3715556/1‑91 (MQ=255) gACATATCGGATAAATGATCAACATCATGTTGCACATCTTTATCAATATTATTATCAGCATCTTTAACCACGTCAGACTCTGGTTCTATca < 2:3651989/91‑1 (MQ=255) gACATATCGGATAAATGATCAACATCATGTTGCACATCTTTATCAATATTATTATCAGCATCTTTAACCACGGCAGACTCTGGTTCTATca < 2:3739339/91‑1 (MQ=255) gACATATCGGACAAATGATCAACATCATGTTGCACATCTTTATCAATATTATTATCAGCATCTTTAACCACGTCAGACTCTGGTTCTATca < 1:3521370/91‑1 (MQ=255) aCATATCGGATAAATGATCAACATCATGTTGCACATCTTTATCAATATTATTATCAGCATCTTTAACCACGTCAGACTCTGGTTCTATcac > 2:2540543/1‑91 (MQ=255) atatCGGATAAATGATCAACATCATGTTGCACATCTTTATCAATATTATTATCAGCATCTTTAACCACTTCAGACTCTGGTTCTATCACAt < 1:523103/91‑1 (MQ=255) atatCGGATAAATGATCAACATCATGTTGCACATCTTTATCAATATTATTATCAGCATCTTTAACCACGTCAGACTCTGGTTCTATCACAt < 1:1145419/91‑1 (MQ=255) tatCGGATAAATGGTCAACATCATGTTGCACATCTTTATCAATATTATTATCAGCATCTTTAACCACGTCAGACTCTGGTTCTATCACATc < 2:2015379/91‑1 (MQ=255) tatCGGATAAATGATCAACATCATGTTGCACATCTTTATCAATATTATTATCAGCATCTTTAACCACGTCAGACTCTGGTTCTATCACATc < 2:2015934/91‑1 (MQ=255) tatCGGATAAATGATCAACATCATGTTGCACATCTTTATCAATATTATTATCAGCATCTTTAACCACGTCAGACTCTGGTTCTATCACATc < 1:1711678/91‑1 (MQ=255) tatCGGATAAATGATCAACATCATGTTGCACATCTTTATCAATATTATTATCAGCATCTTTAACCACGTCAGACTCTGGTTCTATCACATc < 1:1711410/91‑1 (MQ=255) tatCGGATAAATGATCAACATCATGTTGCACATCTTTATCAATATTATTATCAGCATCTTTAACCACGTCAGACTCTGGTTCTAGCACATc < 1:3023140/91‑1 (MQ=255) atCGGATAAATGATCAACATCATGTTGCACATCTTTATCAATATTATTATCAGCATCTTTAACCACGTCAGACTCTGGTTCTATCACATCt < 2:3486868/91‑1 (MQ=255) | ATCTTTATCCACATTTCTATCAGTATCTTTGGCAATTTGAGTATCCATTTCTTTTAAATCATATTTATCGAAGTGATTA‑TTATCCGACATATCGGATAAATGATCAACATCATGTTGCACATCTTTATCAATATTATTATCAGCATCTTTAACCACGTCAGACTCTGGTTCTATCACATCT > USA300TCH1516_ALE/1845205‑1845385 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |