Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 1,929,565 C→T 5.6% D34N (GAT→AAT)  USA300TCH1516_01793 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,929,5650CT 5.6% 898.7 / 24.7 267D34N (GAT→AAT) USA300TCH1516_01793hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (121/131);  new base T (6/9);  total (127/140)
Fisher's exact test for biased strand distribution p-value = 6.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

Only 100 of 275 total aligned reads displayed.

GGTGATTGAATGTACCCGTGTTTTGATGTTGCTTTATCCATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACTTAAACTCTGAACTAAATGATCTTCTTTC  >  USA300TCH1516_ALE/1929476‑1929655
                                                                                         |                                                                                          
 gTGATTGAATGTACCCGTGTTTTGATGTTGCTTTATCCATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCAt                                                                                          >  1:2957903/1‑91 (MQ=255)
 gTGATTGAATGTACCCGTGTTTTGATGTTGCGTTATCCATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCAt                                                                                          <  1:1046424/91‑1 (MQ=255)
       gAATGTACCCGTGTTTTGATGTTGCTTTATCCATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTcgc                                                                                    >  2:2848238/1‑91 (MQ=255)
        aaTGTACCCGTGTTTTGATGTTGCTTTATCCATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTcgcg                                                                                   >  2:2368654/1‑91 (MQ=255)
         aTGTACCCGTGTTTTGATGTTGCTTTATCCATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGt                                                                                  <  1:1950791/91‑1 (MQ=255)
          tGTACCCGTGTTTTGATGTTGCTTTATCCATTATCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGta                                                                                 >  2:3804991/1‑91 (MQ=255)
            tACCCGTGTTTTGATGTTGCTTTATCCATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGtata                                                                               <  1:2204413/91‑1 (MQ=255)
            tACCCGTGTTTTGATGTTGCTTTATCCATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGtata                                                                               >  2:2919518/1‑91 (MQ=255)
             aCCCGTGTTTTGATGTTGCTTTATCCATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATaa                                                                              >  2:2020831/1‑91 (MQ=255)
             aCCCGTGTTTTGATGTTGCTTTATCCATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATaa                                                                              <  1:49397/91‑1 (MQ=255)
              cccGTGTTTTGATGTTGCTTTATCCATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATTATACGCGAGtatgat                                                                             <  2:3582579/91‑4 (MQ=255)
              cccGTGTTTTGATGTTGCTTTATCCATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAAt                                                                             >  1:3652106/1‑91 (MQ=255)
               ccGTGTTTTGATGTTGCTTTATCCATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAAtt                                                                            <  1:1052311/91‑1 (MQ=255)
                cGTGTTTTGATGTTGCTTTATCCATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAAttt                                                                           >  2:3296122/1‑91 (MQ=255)
                cGTGTTTTGATGTTGCTTTATCCATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAAttt                                                                           <  1:1551952/91‑1 (MQ=255)
                cGTGTTTTGATGTTGCTTTATCCATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAAttt                                                                           >  1:968001/1‑91 (MQ=255)
                 gtgtTTTGATGTTGCTTTATCCATTTTCCTACATTTTATACCCGCCATTTACAAACGTCGAAGGGGTGAAATCATACTCGCGTATAATTTc                                                                          <  2:1011517/91‑1 (MQ=255)
                 gtgtTTTGATGTTGCTTTATCCATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTc                                                                          <  1:2762201/91‑1 (MQ=255)
                 gtgtTTTGATGTTGCTTTATCCATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTc                                                                          <  1:838933/91‑1 (MQ=255)
                 gtgtTTTGATGTTGCTTTATCCATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTc                                                                          >  1:1340113/1‑91 (MQ=255)
                 gtgtTTTGATGTTGCTTTATCCATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTc                                                                          <  2:1734129/91‑1 (MQ=255)
                 gtgtTTTGATGTTGCTTTATCCATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTc                                                                          >  2:2239210/1‑91 (MQ=255)
                    ttttGATGTTGCTTTATCCATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGta                                                                                 >  1:609965/1‑81 (MQ=255)
                     tttGATGTTGCTTTATCCATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTc                                                                      >  1:3343462/1‑91 (MQ=255)
                       tGATGTTGCTTTATCCATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCt                                                                    >  2:53898/1‑91 (MQ=255)
                        gATGTTGCTTTATCCATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTa                                                                   >  1:369824/1‑91 (MQ=255)
                        gATGTTGCTTTATCCATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTa                                                                   >  1:914509/1‑91 (MQ=255)
                        gATGTTGCTTTATCCATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTa                                                                   >  2:2086819/1‑91 (MQ=255)
                         aTGTTGCTTTATCCATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAg                                                                  >  1:1299975/1‑91 (MQ=255)
                          tGTTGCTTTATCCATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAgg                                                                 >  1:2162342/1‑91 (MQ=255)
                          tGTTGCTTTATCCATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAgg                                                                 >  2:3715806/1‑91 (MQ=255)
                            ttGCTTTATCCATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCt                                                               >  2:2716136/1‑91 (MQ=255)
                            ttGCTTTATCCATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCt                                                               >  2:2487827/1‑91 (MQ=255)
                            ttGCTTTATCCATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCt                                                               <  1:3290304/91‑1 (MQ=255)
                             tGCTTTATCCATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCtt                                                              <  2:1362486/91‑1 (MQ=255)
                             tGCTTTATCCATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCtt                                                              <  1:1103211/91‑1 (MQ=255)
                               cTTTATCCATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTAc                                                            <  2:3125412/91‑1 (MQ=255)
                               cTTTATCCATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTAc                                                            <  1:3773839/91‑1 (MQ=255)
                                tttATCCATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTAcc                                                           <  1:1004179/91‑1 (MQ=255)
                                 ttATCCATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCa                                                          >  1:1897777/1‑91 (MQ=255)
                                  tATCCATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCAt                                                         >  1:3196896/1‑91 (MQ=255)
                                    tCCATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCAttt                                                       >  2:3015815/1‑91 (MQ=255)
                                    tCCATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCAttt                                                       >  2:3032788/1‑91 (MQ=255)
                                     ccATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCAtttt                                                      <  1:1675842/91‑1 (MQ=255)
                                      cATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCAttttt                                                     <  2:1102165/91‑1 (MQ=255)
                                        ttttCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTtat                                                   >  1:2902388/1‑91 (MQ=255)
                                        ttttCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTtat                                                   >  1:2195272/1‑91 (MQ=255)
                                            ccTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATaa                                               <  2:2548746/91‑1 (MQ=255)
                                             cTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAAt                                              <  1:1442593/91‑1 (MQ=255)
                                               aCATTTTGTAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCt                                            <  2:3453818/91‑1 (MQ=255)
                                               aCATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCt                                            >  1:1917667/1‑91 (MQ=255)
                                               aCATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCt                                            >  2:1817294/1‑91 (MQ=255)
                                               aCATTTTATAACCGCCATTTACAAACGTCGAAGCTGTGAAATCATACTCGCGTATAATTACCTTCCTAGGCTTACCATTTTTATATAATCt                                            >  1:3105140/1‑91 (MQ=255)
                                                cATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTa                                           >  2:452773/1‑91 (MQ=255)
                                                  ttttATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAAc                                         <  1:2692626/91‑1 (MQ=255)
                                                     tataACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCAt                                      >  1:2363639/1‑91 (MQ=255)
                                                      ataACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCAtt                                     >  1:3220346/1‑91 (MQ=255)
                                                      ataACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCAtt                                     >  1:3557111/1‑91 (MQ=255)
                                                       taACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCAttt                                    >  1:1862151/1‑91 (MQ=255)
                                                       taACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCAttt                                    >  1:940463/1‑91 (MQ=255)
                                                        aaCCGCCATTTACAAACGTCGAAGGTGTGACATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCAGTTg                                   >  1:2370878/1‑91 (MQ=255)
                                                        aaCCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTg                                   <  1:3037180/91‑1 (MQ=255)
                                                         aCCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGt                                  <  1:3848303/91‑1 (MQ=255)
                                                           cGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTaa                                <  2:3507543/91‑1 (MQ=255)
                                                            gCCATTTACAAACGTCGCAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAAc                               <  1:3329109/91‑1 (MQ=255)
                                                             ccATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACt                              <  2:185227/91‑1 (MQ=255)
                                                              cATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACtt                             >  1:1383709/1‑91 (MQ=255)
                                                               aTTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACTTa                            >  2:1447007/1‑91 (MQ=255)
                                                               aTTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACTTa                            <  1:1635907/91‑1 (MQ=255)
                                                               aTTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACTTa                            <  1:1258096/91‑1 (MQ=255)
                                                                 ttACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACTTaaa                          >  1:42843/1‑91 (MQ=255)
                                                                  tACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACTTAAAc                         >  1:2377504/1‑91 (MQ=255)
                                                                  tACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACTTAAAc                         >  2:2669754/1‑91 (MQ=255)
                                                                  tACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACTTAAAc                         >  1:2275716/1‑91 (MQ=255)
                                                                   aCAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACTTAAAct                        >  2:615560/1‑91 (MQ=255)
                                                                      aaCGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTACCTTAAACTCTg                     >  1:3518180/1‑91 (MQ=255)
                                                                      aaCGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACTTAAACTCTg                     <  2:1313769/91‑1 (MQ=255)
                                                                       aCGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACTTAAACTCTGa                    >  2:2293538/1‑91 (MQ=255)
                                                                        cGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACTTAAACTCTGaa                   >  1:833717/1‑91 (MQ=255)
                                                                         gTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACTTAAACTCTGAAc                  <  2:961944/91‑1 (MQ=255)
                                                                           ctaaGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACTTAAACTCTGAACTa                <  2:1655640/89‑1 (MQ=255)
                                                                           cGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACTTAAACTCTGAACTa                <  2:3873051/91‑1 (MQ=255)
                                                                            gAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACTTAAACTCTGAACTaa               >  1:2850180/1‑91 (MQ=255)
                                                                             aaGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACTTAAACTCTGAACTaaa              <  2:3549139/91‑1 (MQ=255)
                                                                             aaGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACTTAAACTCTGAACTaaa              <  1:1687054/91‑1 (MQ=255)
                                                                              aGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACTTAAACTCTGAACTAAAt             >  2:578152/1‑91 (MQ=255)
                                                                               ggTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACTTAAACTCTGAACTAAATg            <  2:3153009/91‑1 (MQ=255)
                                                                                  gtgAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACTTAAACTCTGAACTAAATGAtc         <  1:1985163/91‑1 (MQ=255)
                                                                                    gAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTATCCATTTGTACCTTAAACTCTGAACTAAATGAtctt       <  2:256948/91‑1 (MQ=255)
                                                                                    gAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAAc                               <  2:955859/67‑1 (MQ=255)
                                                                                    gAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAAc                               >  1:957629/1‑67 (MQ=255)
                                                                                    gAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACTTAAACTCTGAACTAAATGAtctt       <  2:2421191/91‑1 (MQ=255)
                                                                                    gAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACTTAAACTCTGAACTAAATGAtctt       <  1:3627729/91‑1 (MQ=255)
                                                                                     aaaTTATACGCGAGTATGATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACTTAAACTCTGAACTAAATGAtcttc      >  2:2839773/1‑91 (MQ=14)
                                                                                     aaaTCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACTTAAACTCTGAACTAAATGAtcttc      <  2:902940/91‑1 (MQ=255)
                                                                                     aaaTCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACTTAAACTCTGAACTAAATGAtcttc      <  1:1479528/91‑1 (MQ=255)
                                                                                      aaTCATACTCGCGTATAATTTCATTTATTGGCTTACCATTTTCATATAATCTAACCATTTATAACTTAAACTCTGAACGAAATGTtcttct     >  2:3220157/1‑91 (MQ=14)
                                                                                      aaTCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACTTAAACTCTGAACTAAATGAtcttct     >  2:834929/1‑91 (MQ=255)
                                                                                         cATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACTTAAACTCTGAACTAAATGATCttctttc  >  2:2119723/1‑91 (MQ=255)
                                                                                         cATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACTTAAACTCTGAACTAAATGATCttctttc  <  2:2461336/91‑1 (MQ=255)
                                                                                         |                                                                                          
GGTGATTGAATGTACCCGTGTTTTGATGTTGCTTTATCCATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACTTAAACTCTGAACTAAATGATCTTCTTTC  >  USA300TCH1516_ALE/1929476‑1929655

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑