Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 1,929,578 A→G 5.5% I29I (ATT→ATC)  USA300TCH1516_01793 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,929,5780AG 5.5% 886.8 / 25.4 270I29I (ATT→ATC) USA300TCH1516_01793hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (134/121);  new base G (8/7);  total (142/128)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 285 total aligned reads displayed.

TACCCGTGTTTTGATGTTGCTTTATCCATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACTTAAACTCTGAACTAAATGATCTTCTTTCTCTTGTCATAAT  >  USA300TCH1516_ALE/1929488‑1929667
                                                                                          |                                                                                         
tACCCGTGTTTTGATGTTGCTTTATCCATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGtata                                                                                           >  2:2919518/1‑91 (MQ=255)
 aCCCGTGTTTTGATGTTGCTTTATCCATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATaa                                                                                          <  1:49202/91‑1 (MQ=255)
  cccGTGTTTTGATGTTGCTTTATCCATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAAt                                                                                         >  1:2942240/1‑91 (MQ=255)
   ccGTGTTTTGATGTTGCTTTATCCATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAAtt                                                                                        <  1:1052311/91‑1 (MQ=255)
   ccGTGTTTTGATGTTGCTTTATCCATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAAtt                                                                                        <  1:856517/91‑1 (MQ=255)
    cGTGTTTTGATGTTGCTTTATCCATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATTATACGCGAGTATGAttt                                                                                       <  2:629400/91‑1 (MQ=38)
    cGTGTTTTGATGTTGCTTTATCCATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAAttt                                                                                       >  2:2346311/1‑91 (MQ=255)
    cGTGTTTTGATGTTGCTTTATCCATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAAttt                                                                                       >  2:3296121/1‑91 (MQ=255)
    cGTGTTTTGATGTTGCTTTATCCATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAAttt                                                                                       >  1:968001/1‑91 (MQ=255)
     gtgtTTTGATGTTGCTTTATCCATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTc                                                                                      >  1:1340113/1‑91 (MQ=255)
     gtgtTTTGATGTTGCTTTATCCATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTc                                                                                      >  1:1529640/1‑91 (MQ=255)
     gtgtTTTGATGTTGCTTTATCCATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTc                                                                                      <  1:2762201/91‑1 (MQ=255)
         tttGATGTTGCTTTATCCATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATTATACGCGAGTATGATTTCacac                                                                                  <  1:954253/91‑4 (MQ=37)
          ttGATGTTGCTTTATCCATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTcc                                                                                 >  2:2089197/1‑91 (MQ=255)
           tGATGTTGCTTTATCCATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCt                                                                                >  2:53898/1‑91 (MQ=255)
             aTGTTGCTTTATCCATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAg                                                                              >  1:1299975/1‑91 (MQ=255)
              tGTTGCTTTATCCATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAgg                                                                             >  1:3825512/1‑91 (MQ=255)
               gTTGCTTTATCCATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGc                                                                            <  1:2140165/91‑1 (MQ=255)
                ttGCTTTATCCATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCt                                                                           <  1:3290304/91‑1 (MQ=255)
                 tGCTTTATCCATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCtt                                                                          <  1:1103211/91‑1 (MQ=255)
                 tGCTTTATCCATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCtt                                                                          <  1:1419477/91‑1 (MQ=255)
                 tGCTTTATCCATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCtt                                                                          <  1:1883552/91‑1 (MQ=255)
                 tGCTTTATCCATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCtt                                                                          <  1:922739/91‑1 (MQ=255)
                 tGCTTTATCCATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCtt                                                                          <  2:2469584/91‑1 (MQ=255)
                  gCTTTATCCATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTa                                                                         <  2:3144636/91‑1 (MQ=255)
                  gCTTTATCCATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTa                                                                         >  2:2030768/1‑91 (MQ=255)
                    tttATCCATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTAcc                                                                       <  1:1004179/91‑1 (MQ=255)
                    tttATCCATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTAcc                                                                       >  1:3207333/1‑91 (MQ=255)
                          cATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCAttttt                                                                 <  2:1102165/91‑1 (MQ=255)
                            ttttCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTtat                                                               >  1:2902388/1‑91 (MQ=255)
                            ttttCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTtat                                                               >  1:2195575/1‑91 (MQ=255)
                             tttCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTtata                                                              >  1:3687031/1‑91 (MQ=255)
                             tttCCTACATTTTATAACCGCCATTTACAAACGGCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTtata                                                              <  1:3230906/91‑1 (MQ=255)
                               tCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTtatata                                                            <  1:2163263/91‑1 (MQ=255)
                                ccTACATTTTATAACCGCCATTTACAAACTTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATaa                                                           <  2:2548907/91‑1 (MQ=255)
                                ccTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATaa                                                           <  2:1997600/91‑1 (MQ=255)
                                ccTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATaa                                                           <  1:3515184/91‑1 (MQ=255)
                                 cTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAAt                                                          >  1:2316668/1‑91 (MQ=255)
                                   aCATTTTGTAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCt                                                        <  2:3453818/91‑1 (MQ=255)
                                   aCATTTTATAACCGCCATTTACAAACGTCGAAGCTGTGAAATCATACTCGCGTATAATTACCTTCCTAGGCTTACCATTTTTATATAATCt                                                        >  1:3105140/1‑91 (MQ=255)
                                    cATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTa                                                       >  2:1066023/1‑91 (MQ=255)
                                    cATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTa                                                       >  2:452773/1‑91 (MQ=255)
                                      ttttATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAAc                                                     <  1:2692626/91‑1 (MQ=255)
                                       tttATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAAcc                                                    <  1:534799/91‑1 (MQ=255)
                                          ataACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCAtt                                                 >  1:3557111/1‑91 (MQ=255)
                                           taACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCAttt                                                >  2:2841049/1‑91 (MQ=255)
                                            aaCCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTg                                               <  1:1950053/91‑1 (MQ=255)
                                            aaCCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTg                                               <  1:3037180/91‑1 (MQ=255)
                                               cGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTaa                                            <  2:3507543/91‑1 (MQ=255)
                                                gCCATTTACAAACGTCGCAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAAc                                           <  1:3329109/91‑1 (MQ=255)
                                                gCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAAc                                           <  2:1457580/91‑1 (MQ=255)
                                                 ccATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACt                                          <  2:185227/91‑1 (MQ=255)
                                                 ccATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTCGTAACt                                          >  2:2816120/1‑91 (MQ=255)
                                                  cATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACtt                                         >  1:1383709/1‑91 (MQ=255)
                                                   aTTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACTTa                                        >  1:1451781/1‑91 (MQ=255)
                                                   aTTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACTTa                                        <  1:1635869/91‑1 (MQ=255)
                                                   aTTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACTTa                                        <  1:3737089/91‑1 (MQ=255)
                                                      tACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACTTAAAc                                     >  2:2669754/1‑91 (MQ=255)
                                                      tACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACTTAAAc                                     >  1:2377504/1‑91 (MQ=255)
                                                          aaCGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACTTAAACTCTg                                 <  2:1313769/91‑1 (MQ=255)
                                                          aaCGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTATTATATAATCTAACCATTTATACCTTAAACTCTg                                 >  1:3518680/1‑91 (MQ=255)
                                                           atggcGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACTTAAACTCTGa                                >  2:2199890/5‑91 (MQ=255)
                                                            cGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACTTAAACTCTGaa                               >  1:833717/1‑91 (MQ=255)
                                                             gTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACTTAAACTCTGAAc                              <  2:961944/91‑1 (MQ=255)
                                                               ctaaGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACTTAAACTCTGAACTa                            <  2:1655640/89‑1 (MQ=255)
                                                               cGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACTTAAACTCTGAACTa                            <  2:3873051/91‑1 (MQ=255)
                                                                gAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACTTAAACTCTGAACTaa                           >  1:2850180/1‑91 (MQ=255)
                                                                 aaGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACTTAAACTCTGAACTaaa                          <  1:1687054/91‑1 (MQ=255)
                                                                  aGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACTTAAACTCTGAACTAAAt                         >  2:578152/1‑91 (MQ=255)
                                                                   ggTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACTTAAACTCTGAACTAAATg                        <  1:1674069/91‑1 (MQ=255)
                                                                    gtgtGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACTTAAACTCTGAACTCAATGa                       >  2:3575724/1‑91 (MQ=255)
                                                                     tgtgAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACTTAAACTCTGAACTAAATGAt                      <  1:2368654/91‑1 (MQ=255)
                                                                      gtgAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACTTAAACTCTGAACTAAATGAtc                     <  1:1985163/91‑1 (MQ=255)
                                                                      gtgAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACTTAAACTCTGAACTAAATGAtc                     >  1:1897958/1‑91 (MQ=255)
                                                                        gAAATCATACTCGCTTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAGCTTAAACTCTGAACTAAATGAtctt                   <  2:1378225/91‑1 (MQ=255)
                                                                        gAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAAc                                           <  2:955859/67‑1 (MQ=255)
                                                                        gAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACTTAAACTCTGAACTAAATGAtctt                   <  2:2421245/91‑1 (MQ=255)
                                                                        gAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACTTAAACTCTGAACTAAATGAtctt                   <  1:3627978/91‑1 (MQ=255)
                                                                         aaaTCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACTTAAACTCTGAACTAAATGAtcttc                  <  2:902940/91‑1 (MQ=255)
                                                                          aaTCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACTTAAACTCTGAACTAAATGAtcttct                 <  1:678825/91‑1 (MQ=255)
                                                                          aaTCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACTTAAACTCTGAACTAAATGAtcttct                 <  1:3856257/91‑1 (MQ=255)
                                                                           aTCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACTTAAACTCTGAACTAAATGATcttctt                >  2:1736438/1‑91 (MQ=255)
                                                                           aTCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACTTAAACTCTGAACTAAATGATcttctt                >  2:2488497/1‑91 (MQ=255)
                                                                             cATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACTTAAACTCTGAACTAAATGATCttctttc              >  1:2266324/1‑91 (MQ=255)
                                                                             cATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACTTAAACTCTGAACTAAATGATCttctttc              >  2:3742186/1‑91 (MQ=255)
                                                                              aTACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACTTAAACTCTGAACTAAATGATCttctttct             <  2:1792952/91‑1 (MQ=255)
                                                                               tACTCGCGTATGATTTCATTCCTGGGCTTACCATTTTTATATAATCTAAGCATTTGTAAGTTAAACTCTGAACTAAATGATCTTCTTtctc            <  2:2956056/91‑1 (MQ=17)
                                                                               tACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACTTAAACTCTGAACTAAATGATCTTCTTtctc            >  1:1840416/1‑91 (MQ=255)
                                                                                aCTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTCACTTAAACTCTGAACTAAATGATCTTCTTTctct           >  2:3243690/1‑91 (MQ=255)
                                                                                aCTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACTTAAACTCTGAACTAAATGATCTTCTTTctct           >  2:3033231/1‑91 (MQ=255)
                                                                                aCTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACTTAAACTCTGAACTAAATGATCTTCTTTctct           >  2:3032507/1‑91 (MQ=255)
                                                                                 cTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACTTAAACTCTGAACTAAATGATCTTCTTTCTCtt          >  1:3179432/1‑91 (MQ=255)
                                                                                  gcgagTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACTTAAACTCTGAACTAAATGATCTTCTTTCTCTTg         >  1:2039724/5‑91 (MQ=255)
                                                                                  gcgagTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACTTAAACTCTGAACTAAATGATCTTCTTTCTCTTg         >  1:2436337/5‑91 (MQ=255)
                                                                                    gcgTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACTTAAACTCTGAACTAAATGATCTTCTTTCTCTTGTc       <  2:2695713/91‑1 (MQ=255)
                                                                                    gcgTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACTTAAACTCTGAACTAAATGATCTTCTTTCTCTTGTc       <  1:3346579/91‑1 (MQ=255)
                                                                                        ataATTTCATTCCTAGGCTTACCATTTTTATATAATCTACCCATTTGTAACTTAAACTCTGAACTAAATGATCTTCTTTCTCTTGTCataa   <  1:1031446/91‑1 (MQ=255)
                                                                                        ataATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACTTAAACTCTGAACTAAATGATCTTCTTTCTCTTGTCataa   <  2:3007827/91‑1 (MQ=255)
                                                                                         taATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACTTAAACTCTGAACTAAATGATCTTCTTTCTCTTGTCataat  <  2:1529640/91‑1 (MQ=255)
                                                                                         taATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACTTAAACTCTGAACTAAATGATCTTCTTTCTCTTGTCataat  <  2:2283821/91‑1 (MQ=255)
                                                                                          |                                                                                         
TACCCGTGTTTTGATGTTGCTTTATCCATTTTCCTACATTTTATAACCGCCATTTACAAACGTCGAAGGTGTGAAATCATACTCGCGTATAATTTCATTCCTAGGCTTACCATTTTTATATAATCTAACCATTTGTAACTTAAACTCTGAACTAAATGATCTTCTTTCTCTTGTCATAAT  >  USA300TCH1516_ALE/1929488‑1929667

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑