Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 1,931,599 A→T 9.1% L1217I (TTA→ATA)  USA300HOU_RS09605 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,931,5990AT 9.1% 973.8 / 65.3 305L1217I (TTA→ATA) USA300HOU_RS09605hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (132/145);  new base T (17/11);  total (149/156)
Fisher's exact test for biased strand distribution p-value = 2.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

Only 100 of 348 total aligned reads displayed.

TGTACCACCATTCTTGTCTATCTCGAAGAGAAATTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAATACATATTGTTTCACCAAATTTAGTTTTCCA  >  USA300TCH1516_ALE/1931509‑1931689
                                                                                          |                                                                                          
tGTACCACCATTCTTGTCTATCTCGAAGAGAAATTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTtaa                                                                                            <  1:1237763/91‑1 (MQ=255)
tGTACCACCATTCTTGTCTATCTCGAAGAGAAATTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTtaa                                                                                            >  1:594304/1‑91 (MQ=255)
 gTACCACCATTCTTGTCTATCTCGAAGAGAAATTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTtaat                                                                                           >  2:913318/1‑91 (MQ=255)
 gTACCACCATTCTTGTCTATCTCGAAGAGAAATTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTtaat                                                                                           >  2:3015008/1‑91 (MQ=255)
 gTACCACCATTCTTGTCTATCTCGAAGAGAAATTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTtaat                                                                                           <  1:2703311/91‑1 (MQ=255)
 gTACCACCATTCTTGTCTATCTCGAAGAGAAATTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTtaat                                                                                           >  2:2337750/1‑91 (MQ=255)
   accaccATTCTTGTCTATCTCGAAGAGAAATTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTtattaa                                                                                         <  1:2463490/91‑1 (MQ=255)
    ccaccaTTCTTGTCTATCTCGAAGAGAAATTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATaaa                                                                                        <  2:1829330/91‑1 (MQ=255)
    ccaccaTTCTTGTCTATCTCGAAGAGAAATTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATaaa                                                                                        <  1:2778778/91‑1 (MQ=255)
     caccaTTCTTGTCTATCTCGAAGAGAAATTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAAc                                                                                       <  2:1651136/91‑1 (MQ=255)
     caccaTTCTTGTCTATCTCGAAGAGAAATTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAAc                                                                                       <  1:3475008/91‑1 (MQ=255)
     caccaTTCTTGTCTATCTCGAAGAGAAATTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAAc                                                                                       <  1:3864662/91‑1 (MQ=255)
     caccaTTCTTGTCTATCTCGAAGAGAAATTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAAc                                                                                       <  1:2002812/91‑1 (MQ=255)
     caccaTTCTTGTCTATCTCGAAGAGAAATTTTTTCTAGATATATTTTCCATCCAATAGACAAGTTTTTAGTATTAAGAAAGTTTAATAAAc                                                                                       <  2:1646765/91‑1 (MQ=255)
      accaTTCTTGTCTATCTCGAAGAGAAATTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTATTAAAct                                                                                      <  1:3690747/91‑1 (MQ=255)
      accaTTCTTGTCTATCTCGAAGAGAAATTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTATTAAAct                                                                                      <  1:3205683/91‑1 (MQ=255)
       ccaTTCTTGTCTATCTCGAAGAGAAATTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAActt                                                                                     >  2:2235641/1‑91 (MQ=255)
        caTTCTTGTCTATCTCGAAGAGAAATTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAActtt                                                                                    <  2:1266518/91‑1 (MQ=255)
           tCTTGTCTATCTCGAAGAGAAATTTTTTCTAGATATACTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAAcgttctt                                                                                 >  1:3608936/1‑91 (MQ=255)
            cTTGTCTATCTCGAAGAGAAATTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTATTAAActttctta                                                                                <  1:1857005/91‑2 (MQ=255)
            cTTGTCTATCTCGAAGAGAAATTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAActttcttt                                                                                >  2:546943/1‑91 (MQ=255)
            cTTGTCTATCTCGAAGAGAAATTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAActttcttt                                                                                >  1:500949/1‑91 (MQ=255)
             ttGTCTATCTCGAAGAGAAATTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCtttt                                                                               >  2:2830804/1‑91 (MQ=255)
             ttGTCTATCTCGAAGAGAAATTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCtttt                                                                               >  2:1555467/1‑91 (MQ=255)
                tCTATCTCGAAGAGAAATTTTTTTGAGATATATTTTCAATTCATTGGAAAATTTTTTAGGATTAAGAAAGTTTCATAAAATTTCTTTTTta                                                                            >  2:347681/1‑91 (MQ=255)
                tCTATCTCGAAGAGAAATTTTTTCTATTTATATTTTCCAATCAACAGCCTACTTTTTATTATTCAGTTTGTTTATTAAACTTTTTCTTTta                                                                            >  2:3574470/1‑91 (MQ=255)
                tCTATCTCGAAGAGAAATTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTta                                                                            >  1:3024867/1‑91 (MQ=255)
                  tATCTCGAAGAGAAATTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTtaat                                                                          <  1:1316416/91‑1 (MQ=255)
                      tcGAAGAGAAATTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGt                                                                      >  2:297665/1‑91 (MQ=255)
                        gAAGAGAAATTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTaa                                                                    >  1:2747307/1‑91 (MQ=255)
                        gAAGAGAAATTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTaa                                                                    >  1:2323715/1‑91 (MQ=255)
                        gAAGAGAAATTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTaa                                                                    <  2:2757343/91‑1 (MQ=255)
                           gagaAATTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAAca                                                                 >  1:528647/1‑91 (MQ=255)
                           gagaAATTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAAca                                                                 >  1:1041547/1‑91 (MQ=255)
                           gagaAATTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAAca                                                                 <  1:252275/91‑1 (MQ=255)
                           gagaAATTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAAca                                                                 <  1:166874/91‑1 (MQ=255)
                             gaAATTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAAcatt                                                               >  1:1626561/1‑91 (MQ=255)
                                aTTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAAcattcat                                                            <  2:430824/91‑1 (MQ=255)
                                aTTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAAcattcat                                                            <  2:1035698/91‑1 (MQ=255)
                                 ttttttCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAAcattcatt                                                           <  2:369403/91‑1 (MQ=255)
                                   ttttCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTAt                                                         <  1:1494667/91‑1 (MQ=255)
                                   ttttCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTAt                                                         <  1:747746/91‑1 (MQ=255)
                                   ttttCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTAt                                                         <  2:1022681/91‑1 (MQ=255)
                                       cTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCaaa                                                     >  1:3262987/1‑91 (MQ=255)
                                       cTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCaaa                                                     >  2:586441/1‑91 (MQ=255)
                                         aGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAAtt                                                   >  1:1723677/1‑91 (MQ=255)
                                         aGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAAtt                                                   <  2:2218861/91‑1 (MQ=255)
                                         aGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAAtt                                                   >  1:660664/1‑91 (MQ=255)
                                          gATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTc                                                  <  2:1470065/91‑1 (MQ=255)
                                           atatatTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCg                                                 <  1:1117207/91‑1 (MQ=255)
                                            tatatTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGa                                                <  1:2257689/91‑1 (MQ=255)
                                             atatTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAg                                               <  1:3002121/91‑1 (MQ=255)
                                               atTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTa                                             <  2:738348/91‑1 (MQ=255)
                                                ttttCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTaa                                            >  1:3035325/1‑91 (MQ=255)
                                                 tttCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAAt                                           >  2:3389364/1‑91 (MQ=255)
                                                  ttCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAAtt                                          >  2:2507360/1‑91 (MQ=255)
                                                  ttCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAAtt                                          <  2:714601/91‑1 (MQ=255)
                                                   tccatccaATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTCAttt                                         >  1:3229718/1‑91 (MQ=255)
                                                   tccatccaATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAAttt                                         >  2:2738770/1‑91 (MQ=255)
                                                   tccaaccaATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAAttt                                         <  1:953925/91‑1 (MQ=255)
                                                    ccatccaATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTa                                        <  2:93690/91‑1 (MQ=255)
                                                        ccaATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAAt                                    >  1:3790839/1‑91 (MQ=255)
                                                        ccaATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAAt                                    >  2:2390073/1‑91 (MQ=255)
                                                            tAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAAt                                >  2:3101684/1‑91 (MQ=255)
                                                               aCAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAATACa                             <  1:2849426/91‑1 (MQ=255)
                                                                cAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAATACat                            <  1:1529857/91‑1 (MQ=255)
                                                                 aaTTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAATACata                           >  2:1088219/1‑91 (MQ=255)
                                                                 aaTTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAATACaca                           <  1:2078001/91‑3 (MQ=255)
                                                                          gTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAATACATATTGTTTCAc                  <  1:3577725/91‑1 (MQ=255)
                                                                          gTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAATACATATTGTTTCAc                  >  1:3434999/1‑91 (MQ=255)
                                                                          gTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAATACATATTGTTTCAc                  >  1:1087918/1‑91 (MQ=255)
                                                                            aTTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAATACATATTGTTTCAccc                >  2:3207552/1‑90 (MQ=255)
                                                                            aTTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAATACATATTGTTTCACCa                >  1:3153671/1‑91 (MQ=255)
                                                                              tAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAATACATATTGTTTCACCaaa              <  1:854443/91‑1 (MQ=255)
                                                                              tAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAATACATATTGTTTCACCaaa              <  2:3818612/91‑1 (MQ=255)
                                                                              tAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAATACATATTGTTTCACCaaa              <  1:1073432/91‑1 (MQ=255)
                                                                              tAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAATACATATTGTTTCACCaaa              <  2:176714/91‑1 (MQ=255)
                                                                               aaGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAATACATATTGTTTCACCAAAt             <  2:2119721/91‑1 (MQ=255)
                                                                               aaGAAAGCTTAATAAACTTTCTTTTTTAATTTGTGAACATCCATTATCAAATTCGATTAATTTAGATTTAATTCTCATTTTTTCACCAAAt             >  2:1576601/1‑91 (MQ=255)
                                                                                aGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATAAAATCCGTGTAATTTAGAATTAATACATATTGTTTCACAAAAta            >  2:3872820/1‑90 (MQ=255)
                                                                                 taaaGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAATACATATTGTTTCACCAAAttt           <  1:1179298/90‑1 (MQ=255)
                                                                                 gAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAATACATATTGTTTCACCAAAttt           <  2:659841/91‑1 (MQ=255)
                                                                                 gAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAATACATATTGTTTCACCAAAttt           <  2:1778289/91‑1 (MQ=255)
                                                                                  aaaGTTTATTAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAATACATATTGTTTCACCAAATTTa          >  1:2244777/1‑91 (MQ=255)
                                                                                  aaaGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAATACATATTGTTTCACCAAATTTa          >  1:2400193/1‑91 (MQ=255)
                                                                                    aGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAATACATATTGTTTCACCAAATTTAGt        <  2:3418205/91‑1 (MQ=255)
                                                                                     gTTTATTAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAATACATATTGTTTCACCAAATTTAGtt       >  2:2025110/1‑91 (MQ=255)
                                                                                     gTTTATTAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAATACATATTGTTTCACCAAATTTAGtt       >  2:1396978/1‑91 (MQ=255)
                                                                                     gTTTATTAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAATACATATTGTTTCACCAAATTTAGtt       >  1:688798/1‑91 (MQ=255)
                                                                                     gTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTATGACATATTGTGTCACCAAATTTAGtt       >  2:3355672/1‑91 (MQ=255)
                                                                                     gTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAATACATATTGTTTCACCAAATTTAGtt       >  1:978337/1‑91 (MQ=255)
                                                                                      tttAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAATACATATTGTTTCACCAAATTTAGttt      <  2:1149992/91‑1 (MQ=255)
                                                                                       ttAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAATACATATTGTTTCACCAAATTTAGtttt     <  2:197697/91‑1 (MQ=255)
                                                                                       ttAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAATACATATTGTTTCACCAAATTTAGtttt     >  2:519869/1‑91 (MQ=255)
                                                                                        taataaACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAATACATATTGTTTCACCAAATTTAGTTTTc    <  2:594304/91‑1 (MQ=255)
                                                                                        taataaACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAATACATATTGTTTCACCAAATTTAGTTTTc    >  1:414409/1‑91 (MQ=255)
                                                                                         aataaACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAATACATTTTGTTTCACCAAATTTAGTTTTcc   >  2:2593348/1‑91 (MQ=255)
                                                                                         aataaACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAATACATATTGTTTCACCAAATTTAGTTTTcc   <  1:869252/91‑1 (MQ=255)
                                                                                         aataaACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAATACATATTGTTTCACCAAATTTAGTTTTcc   <  2:3262987/91‑1 (MQ=255)
                                                                                          ataaACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAATACATATTGTTTCACCAAATTTAGTTTTCCa  <  2:3315964/91‑1 (MQ=255)
                                                                                          |                                                                                          
TGTACCACCATTCTTGTCTATCTCGAAGAGAAATTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAATACATATTGTTTCACCAAATTTAGTTTTCCA  >  USA300TCH1516_ALE/1931509‑1931689

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑