BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	2,184,883	G→A	8.4%	T21I (ACA→ATA)	rpsA_2 ←	30S ribosomal protein S1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,184,883	0	G	A	8.4%	662.4 / 33.9	215	T21I (ACA→ATA)	rpsA_2	30S ribosomal protein S1
Reads supporting (aligned to +/- strand): ref base G (88/109); new base A (5/13); total (93/122)
Fisher's exact test for biased strand distribution p-value = 2.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.82e-01

Only 100 of 227 total aligned reads displayed.

ATTTGCTTTATTTGAACTTCATCTAGTCCACCAGTTTGCTCTTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAATTGATTGTCCATTTACTTAACCCACACTT  >  USA300TCH1516_ALE/2184793‑2184973

aTTTGCTTTATTTGAACTTCATCTAGTCCACCAGTTTGCTCTTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAgtg                                                                                            <  2:2383876/91‑1 (MQ=255)

aTTTGCTTTATTTGAACTTCATCTAGTCCACCAGTTTGCTCTTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAgtg                                                                                            >  1:2148402/1‑91 (MQ=255)

 tttGCTTTATTTGAACTTCATCTAGTCCACCAGTTTGCTCTTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAgtgt                                                                                           <  1:840469/91‑1 (MQ=255)

  ttGCTTTATTTGAACTTCATCTAGTCCACCAGTTTGCTCTTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGtt                                                                                          <  1:581574/91‑1 (MQ=255)

   tGCTTTATTTGAACTTCATCTAGTCCACCAGTTTGCTCTTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTa                                                                                         >  2:2230900/1‑91 (MQ=255)

    gCTTTATTTGAACTTCATCTAGTCCACCAGTTTGCTCTTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTaa                                                                                        <  1:1462193/91‑1 (MQ=255)

    gCTTTATTTGAACTTCATCTAGTCCACCAGTTTGCTCTTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTaa                                                                                        <  1:2912859/91‑1 (MQ=255)

     cTTTATTTGAACTTCATCTAGTCCACCAGTTTGCTCTTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAAt                                                                                       >  2:3682677/1‑91 (MQ=255)

     cTTTATTTGAACTTCATCTAGTCCACCAGTTTGCTCTTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAAt                                                                                       >  1:1500965/1‑91 (MQ=255)

     cTTTATTTGAACTTCATCTAGTCCACCAGTTTGCTCTTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAAt                                                                                       <  1:2338455/91‑1 (MQ=255)

     cTTTATTTGAACTGCATCTAGTCCACCAGTTTGCTCTTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTATTaac                                                                                       <  1:3880714/91‑2 (MQ=255)

      tttATTTGAACTTCATCTAGTCCACCAGTTTGCTCTTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATa                                                                                      >  1:3692925/1‑91 (MQ=255)

      tttATTTGAACTTCATCTAGTCCACCAGTTTGCTCTTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTATTaaca                                                                                      <  2:3620951/91‑3 (MQ=255)

          tttGAACTTCATCTAGTCCACCAGTTTGCTCTTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGc                                                                                  >  2:1894204/1‑91 (MQ=255)

           ttGAACTTCATCTAGTCCACCAGTTTGCTCTTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCt                                                                                 <  1:1348594/91‑1 (MQ=255)

            tGAACTTCATCTAGTCCACCAGTTTGCTCTTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCtt                                                                                >  2:959096/1‑91 (MQ=255)

               aCTTCATTTAGTCCACCAGTTTGCTCTTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCaa                                                                             >  2:2555322/1‑91 (MQ=255)

               aCTTCATCTAGTCCACCAGTTTGCTCTTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCaa                                                                             >  2:1536162/1‑91 (MQ=255)

                   cATCTAGTCCACCAGTTTGCTCTTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTg                                                                         >  2:594655/1‑91 (MQ=255)

                     tCTAGTCCACCAGTTTGCTCTTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGtt                                                                       >  2:3286004/1‑91 (MQ=255)

                      cTAGTCCACCAGTTTGCTCTTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGttt                                                                      >  2:1897819/1‑91 (MQ=255)

                       tAGTCCACCAGTTTGCTCTTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGtttt                                                                     <  1:1758726/91‑1 (MQ=255)

                       tAGTCCACCAGTTTGCTCTTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGtttt                                                                     <  1:2694945/91‑1 (MQ=255)

                       tAGTCCACCAGTTTGCTCTTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGtttt                                                                     >  2:3331664/1‑91 (MQ=255)

                       tAGTCCACCAGTTTGCTCTTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGtttt                                                                     <  1:1465993/91‑1 (MQ=255)

                        aGTCCACCAGTTTGCTCTTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGttttt                                                                    <  2:3672919/91‑1 (MQ=255)

                          tCCACCAGTTTGCTCTTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTa                                                                  <  2:812531/91‑1 (MQ=255)

                              ccaGTTTGCTCTTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTaa                                                              >  1:2605514/1‑91 (MQ=255)

                               caGTTTGCTCTTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTaaa                                                             >  2:2128003/1‑91 (MQ=255)

                                aGTTTGCTCTTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAAt                                                            >  2:2154544/1‑91 (MQ=255)

                                 gTTTGCTCTTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAAtt                                                           <  2:2018758/91‑1 (MQ=255)

                                 gTTTGCTCTTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAAtt                                                           <  2:795259/91‑1 (MQ=255)

                                  tttGCTCTTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTg                                                          >  1:53756/1‑91 (MQ=255)

                                   ttGCTCTTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGa                                                         >  1:31398/1‑91 (MQ=255)

                                   ttGCTCTTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGa                                                         <  2:2603528/91‑1 (MQ=255)

                                   ttGCTCTTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGa                                                         >  2:466393/1‑91 (MQ=255)

                                     gCTCTTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGata                                                       >  1:1231919/1‑91 (MQ=255)

                                     gCTCTTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGata                                                       <  2:952776/91‑1 (MQ=255)

                                       tctTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATAtt                                                     >  1:436161/1‑91 (MQ=255)

                                        ctTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATAttt                                                    >  2:2141587/1‑91 (MQ=255)

                                         ttttCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTtc                                                   <  2:3877428/91‑1 (MQ=255)

                                         ttttCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTtc                                                   <  1:468495/91‑1 (MQ=255)

                                           ttCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTtctc                                                 <  2:1449290/91‑1 (MQ=255)

                                             cGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTa                                               <  1:1451040/91‑1 (MQ=255)

                                             cGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTa                                               <  1:1170023/91‑1 (MQ=255)

                                             cGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCACTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTa                                               >  2:2991478/1‑91 (MQ=255)

                                               aTACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATg                                             >  1:1068991/1‑91 (MQ=255)

                                               aTACCTCGCAATAAATGCTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATg                                             <  1:3324074/91‑1 (MQ=255)

                                                tACCTCGCAATAAATGGTACTGTATTTTTTTTTTTTTTTTTTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGa                                            >  2:774561/1‑91 (MQ=255)

                                                tACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTTCTAAATTGATTTTTCTCTATGa                                            >  2:627797/1‑91 (MQ=255)

                                                tACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGa                                            <  1:2971276/91‑1 (MQ=255)

                                                tACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGa                                            <  1:2824035/91‑1 (MQ=255)

                                                 aCCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGAt                                           <  1:2067793/91‑1 (MQ=255)

                                                  ccTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGAtt                                          >  1:3542834/1‑91 (MQ=255)

                                                  ccTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGAtt                                          <  2:1022368/91‑1 (MQ=255)

                                                  ccTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGAtt                                          >  2:183321/1‑91 (MQ=255)

                                                     cGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGaa                                       >  1:1658089/1‑91 (MQ=255)

                                                      gCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAAt                                      >  2:27291/1‑91 (MQ=255)

                                                       cAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGTTTGAAtt                                     >  1:242173/1‑91 (MQ=255)

                                                       cAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAAtt                                     >  1:3036227/1‑91 (MQ=255)

                                                            aaTGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCa                                <  1:2531936/91‑1 (MQ=255)

                                                             aTGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCaa                               <  2:3244672/91‑1 (MQ=255)

                                                               ggTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAatt                             >  1:3833073/1‑91 (MQ=255)

                                                                gTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAattg                            >  2:489512/1‑91 (MQ=255)

                                                                gTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAattg                            <  1:2266088/91‑1 (MQ=255)

                                                                gTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAattg                            <  1:728702/91‑1 (MQ=255)

                                                                 tACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAattga                           >  1:2352378/1‑91 (MQ=255)

                                                                  aCTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAattgat                          <  1:2289152/91‑1 (MQ=255)

                                                                  aCTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAattgat                          <  1:1974584/91‑1 (MQ=255)

                                                                  aCTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTACGATTGAATTAATCAattgat                          <  1:3820487/91‑1 (MQ=255)

                                                                   cTGTATTTTTTTTTTCTTTCTGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAattgatt                         >  2:3861284/1‑91 (MQ=255)

                                                                   cTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAattcaat                         <  1:1095833/91‑5 (MQ=255)

                                                                      tATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAATTGATTGTc                      >  2:1614842/1‑91 (MQ=255)

                                                                      tATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAATTGATTGTc                      >  2:1250872/1‑91 (MQ=255)

                                                                      tATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAATTGATTGTc                      <  1:877036/91‑1 (MQ=255)

                                                                        tttttttCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAATTGATTGTCCa                    >  1:2138672/1‑91 (MQ=255)

                                                                         ttttttCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAATTGATTGTCCAt                   <  2:1242026/91‑1 (MQ=255)

                                                                          tttttCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGACTGAATTAATCAATTGATTGTCCAtt                  <  1:2204570/91‑1 (MQ=255)

                                                                           ttttCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAATTGATTATCCAttt                 >  1:1933920/1‑91 (MQ=255)

                                                                            tttCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAATTGATTGTCCATTTa                <  2:3076615/91‑1 (MQ=255)

                                                                            tttCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAATTGATTGTCCATTTa                <  1:1846075/91‑1 (MQ=255)

                                                                            tttCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAATTGATTGTCCATTTa                >  2:2448094/1‑91 (MQ=255)

                                                                             ttcttcTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAATTGATTGt                       <  2:1560138/83‑1 (MQ=255)

                                                                             ttcttcTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAATTGATTGTCCATTTAc               >  1:1913580/1‑91 (MQ=255)

                                                                              tcttctAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAATTGATTGTCCATTTACt              >  1:555030/1‑91 (MQ=255)

                                                                               cttctAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAATTGATTGTCCATTTACtt             <  1:1536397/91‑1 (MQ=255)

                                                                                 tctAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAATTGATTGTCCATTTACTTaa           >  1:1510905/1‑91 (MQ=255)

                                                                                 tctAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAATTGATTGTCCATTTACTTaa           <  2:1071788/91‑1 (MQ=255)

                                                                                   tAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAATTGATTGTCCATTTACTTAAcc         <  1:3573928/91‑1 (MQ=255)

                                                                                   tAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTAAGATTGAATTAATCAATTGATTGTCCATTTACTTAAcc         <  1:3256360/91‑1 (MQ=255)

                                                                                   aaGCAGTATTAACACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAATTGATTGTCCATTTACTTAAcc         >  2:296807/2‑91 (MQ=255)

                                                                                   aaGCAGTATTAACACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAATTGATTGTCCATTTACTTAAcc         >  2:228102/2‑91 (MQ=255)

                                                                                    aGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAATTGATTGTCCATTTACTTAAccc        <  2:519319/91‑1 (MQ=255)

                                                                                    aGCAGTATTAACACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAATTGATTGTCCATTTACTTAAccc        >  2:947314/1‑91 (MQ=255)

                                                                                     gCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAATTGATTGTCCATTTACTTAACCca       <  2:3167573/91‑1 (MQ=255)

                                                                                      cAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAATTGATTGTCCATTTACTTAACCcac      <  1:1567607/91‑1 (MQ=255)

                                                                                      cAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAATTGATTGTCCATTTACTTAACCcac      <  2:1633820/91‑1 (MQ=255)

                                                                                       aGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAATTGATTGTCCATTTACTTAACCcaca     <  2:2888401/91‑1 (MQ=255)

                                                                                         tgtTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAATTGATTGTCCATTTACTTAACCCACACt   >  2:1436555/1‑91 (MQ=255)

                                                                                          gtTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAATTGATTGTCCATTTACTTAACCCACACtt  >  1:2795394/1‑91 (MQ=255)

ATTTGCTTTATTTGAACTTCATCTAGTCCACCAGTTTGCTCTTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAATTGATTGTCCATTTACTTAACCCACACTT  >  USA300TCH1516_ALE/2184793‑2184973

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`