BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	2,184,888	T→C	6.6%	V19V (GTA→GTG)	rpsA_2 ←	30S ribosomal protein S1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,184,888	0	T	C	6.6%	660.0 / 24.7	212	V19V (GTA→GTG)	rpsA_2	30S ribosomal protein S1
Reads supporting (aligned to +/- strand): ref base T (96/102); new base C (12/2); total (108/104)
Fisher's exact test for biased strand distribution p-value = 1.04e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.59e-01

Only 100 of 224 total aligned reads displayed.

CTTTATTTGAACTTCATCTAGTCCACCAGTTTGCTCTTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAATTGATTGTCCATTTACTTAACCCACACTTTTTCT  >  USA300TCH1516_ALE/2184798‑2184978

cTTTATTTGAACTTCATCTAGTCCACCAGTTTGCTCTTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAAt                                                                                            <  1:2338455/91‑1 (MQ=255)

cTTTATTTGAACTTCATCTAGTCCACCAGTTTGCTCTTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAAt                                                                                            >  2:3682677/1‑91 (MQ=255)

  ttATTTGAACTTCATCTAGTCCACCAGTTTGCTCTTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATAc                                                                                          <  1:55546/91‑1 (MQ=255)

   tATTTGAACTTCATCTAGTCCACCAGTTTGCTCTTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACt                                                                                         <  1:1467077/91‑1 (MQ=255)

    aTTTGAACTTCATCTAGTCCACCAGTTTGCTCTTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTg                                                                                        <  1:423829/91‑1 (MQ=255)

    aTTTGAACTTCATCTAGTCCACCAGTTTGCTCTTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTATTAACACTg                                                                                        <  1:3151368/91‑1 (MQ=255)

     tttGAACTTCATCTAGTCCACCAGTTTGCTCTTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGc                                                                                       >  2:1894204/1‑91 (MQ=255)

      ttGAACTTCATCTAGTCCACCAGTTTGCTCTTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCt                                                                                      <  1:3088151/91‑1 (MQ=255)

      ttGAACTTCATCTAGTCCACCAGTTTGCTCTTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCt                                                                                      <  1:1508207/91‑1 (MQ=255)

      ttGAACTTCATCTAGTCCACCAGTTTGCTCTTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCt                                                                                      >  1:117766/1‑91 (MQ=255)

       tGAACTTCATCTAGTCCACCAGTTTGCTCTTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCtt                                                                                     >  2:959096/1‑91 (MQ=255)

        gAACTTCATCTAGTCCACCAGTTTGCTCTTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTc                                                                                    <  2:2254423/91‑1 (MQ=255)

          aCTTCATCTAGTCCACCAGTTTGCTCTTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCaa                                                                                  >  2:1536162/1‑91 (MQ=255)

              cATCTAGTCCACCAGTTTGCTCTTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTg                                                                              >  2:594655/1‑91 (MQ=255)

                  tAGTCCACCAGTTTGCTCTTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGtttt                                                                          <  1:1758726/91‑1 (MQ=255)

                  tAGTCCACCAGTTTGCTCTTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGtttt                                                                          <  1:3466758/91‑1 (MQ=255)

                  tAGTCCACCAGTTTGCTCTTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGtttt                                                                          <  1:1465993/91‑1 (MQ=255)

                  tAGTCCACCAGTTTGCTCTTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGtttt                                                                          <  1:2694945/91‑1 (MQ=255)

                  tAGTCCACCAGTTTGCTCTTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGtttt                                                                          <  2:665688/91‑1 (MQ=255)

                    gTCCACCAGTTTGCTCTTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGtttttt                                                                        >  2:2490391/1‑91 (MQ=255)

                     tCCACCAGTTTGCTCTTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTa                                                                       <  2:812531/91‑1 (MQ=255)

                         ccaGTTTGCTCTTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTaa                                                                   >  1:2605514/1‑91 (MQ=255)

                          caGTTTGCTCTTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTaaa                                                                  >  2:2128003/1‑91 (MQ=255)

                           aGTTTGCTCTTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAAt                                                                 >  2:2801988/1‑91 (MQ=255)

                            gTTTGCTCTTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTGAAtt                                                                <  2:3681499/91‑1 (MQ=255)

                            gTTTGCTCTTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAAtt                                                                <  2:795259/91‑1 (MQ=255)

                             tttGCTCTTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTg                                                               >  1:1984338/1‑91 (MQ=255)

                             tttGCTCTTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTg                                                               >  1:53756/1‑91 (MQ=255)

                              ttGCTCTTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGa                                                              <  2:2603528/91‑1 (MQ=255)

                              ttGCTCTTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGa                                                              >  2:468495/1‑91 (MQ=255)

                                gCTCTTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGata                                                            <  2:952776/91‑1 (MQ=255)

                                   ctTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATAttt                                                         >  2:2141781/1‑91 (MQ=255)

                                   ctTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATAttt                                                         >  2:2139551/1‑91 (MQ=255)

                                   ctTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATAttt                                                         >  1:3629452/1‑91 (MQ=255)

                                    ttttCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTtc                                                        <  1:468495/91‑1 (MQ=255)

                                    ttttCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTtc                                                        <  2:3877428/91‑1 (MQ=255)

                                    ttttCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTtc                                                        <  2:1835765/91‑1 (MQ=255)

                                       tCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTctct                                                     <  2:3263405/91‑1 (MQ=255)

                                        cGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTa                                                    <  1:1580569/91‑1 (MQ=255)

                                        cGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTa                                                    <  2:1178428/91‑1 (MQ=255)

                                         gATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTAt                                                   >  2:2910045/1‑91 (MQ=255)

                                          aTACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATg                                                  >  1:1068991/1‑91 (MQ=255)

                                          aTACCTCGCAATAAATGCTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATg                                                  <  1:3324074/91‑1 (MQ=255)

                                           tACCTCGCAATAAATGGTACTGTATTTTTTTTTTTTTTTTTTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGa                                                 >  2:774561/1‑91 (MQ=255)

                                           tACCTCGCAATAAATGGTACTGTATTTTTTTCTTTTATCTGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGTTATTTCTCTATgt                                                 >  2:2421453/1‑90 (MQ=255)

                                           tACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGa                                                 <  1:2971276/91‑1 (MQ=255)

                                             ccTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGAtt                                               <  1:137196/91‑1 (MQ=255)

                                                cGCAATAAATGGTACTGTATTTTTTTTTTTTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGaa                                            >  2:2979113/1‑91 (MQ=255)

                                                cGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGaa                                            >  1:1658089/1‑91 (MQ=255)

                                                cGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGaa                                            <  1:3741443/91‑1 (MQ=255)

                                                  cAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAAtt                                          >  1:242312/1‑91 (MQ=255)

                                                  cAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAAtt                                          >  1:242105/1‑91 (MQ=255)

                                                   aaTAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTa                                         <  1:3861723/91‑1 (MQ=255)

                                                       aaTGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCa                                     <  2:38893/91‑1 (MQ=255)

                                                        aTGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCaa                                    <  2:3244672/91‑1 (MQ=255)

                                                          ggTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAatt                                  >  1:126947/1‑91 (MQ=255)

                                                           gTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAattg                                 <  1:2266088/91‑1 (MQ=255)

                                                           gTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAattg                                 <  1:728702/91‑1 (MQ=255)

                                                            tACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAattga                                <  2:2658619/91‑1 (MQ=255)

                                                            tACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAattga                                >  1:2352378/1‑91 (MQ=255)

                                                             aCTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAattgat                               <  1:3820555/91‑1 (MQ=255)

                                                             aCTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAattgat                               <  1:2753047/91‑1 (MQ=255)

                                                              cTGTATTTTTTTTTTCTTTCTGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAattgatt                              >  2:3861284/1‑91 (MQ=255)

                                                              cTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAattcaat                              <  1:1095833/91‑5 (MQ=255)

                                                                 tATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAATTGATTGTc                           >  2:1614842/1‑91 (MQ=255)

                                                                 tATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAATTGATTGTc                           >  2:1250739/1‑91 (MQ=255)

                                                                 tATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAATTGATTGTc                           <  1:877036/91‑1 (MQ=255)

                                                                   tttttttCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAATTGATTGTCCa                         >  1:2138683/1‑91 (MQ=255)

                                                                    ttttttCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAATTGATTGTCCAt                        <  2:615140/91‑1 (MQ=255)

                                                                    ttttttCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAATTGATTGTCCAt                        <  2:1051248/91‑1 (MQ=255)

                                                                     tttttCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGACTGAATTAATCAATTGATTGTCCAtt                       <  1:2204570/91‑1 (MQ=255)

                                                                      ttttCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAATTGATTATCCAttt                      >  1:1933920/1‑91 (MQ=255)

                                                                       tttCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAATTGATTGTCCATTTa                     <  2:3076453/91‑1 (MQ=255)

                                                                       tttCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAATTGATTGTCCATTTa                     <  1:1846075/91‑1 (MQ=255)

                                                                        ttcttcTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAATTGATTGt                            >  1:1560138/1‑83 (MQ=255)

                                                                        ttcttcTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAATTGATTGt                            <  2:1560138/83‑1 (MQ=255)

                                                                        ttcttcTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAATTGATTGTCCATTTAc                    >  1:1913580/1‑91 (MQ=255)

                                                                         tcttctCGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAATTGATTGTCCATTTACt                   >  1:3018484/1‑91 (MQ=255)

                                                                         tcttctAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAATTGATTGTCCATTTACt                   >  2:134594/1‑91 (MQ=255)

                                                                            tctAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAATTGATTGTCCATTTACTTaa                <  2:2587144/91‑1 (MQ=255)

                                                                             gaaGCAGTATTAACACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAATTGATTGTCCATTTACTTAAc               >  2:3651320/3‑91 (MQ=255)

                                                                              tAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAATTGATTGTCCATTTACTTAAcc              <  1:3573915/91‑1 (MQ=255)

                                                                               aGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAATTGATTGTCCATTTACTTAAccc             <  2:519319/91‑1 (MQ=255)

                                                                               aGCAGTATTAACACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAATTGATTGTCCATTTACTTAAccc             >  2:947314/1‑91 (MQ=255)

                                                                                gCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAATTGATTGTCCATTTACTTAACCca            <  2:3167573/91‑1 (MQ=255)

                                                                                 cAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAATTGATTGTCCATTTACTTAACCcac           <  2:1633820/91‑1 (MQ=255)

                                                                                   gtattAACACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAATTGATTGTCCATTTACTTAACCCacac         >  1:828046/4‑91 (MQ=255)

                                                                                    tgtTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAATTGATTGTCCATTTACTTAACCCACACt        >  2:1436555/1‑91 (MQ=255)

                                                                                    tgtTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAATTGATTGTCCATTTACTTAACCCACACt        >  1:2284610/1‑91 (MQ=255)

                                                                                    tattAACACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAATTGATTGTCCATTTACTTAACCCACACt        >  1:1520589/3‑91 (MQ=255)

                                                                                     gtTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAATTGATTGTCCATTTACTTAACCCACACtt       >  1:2796114/1‑91 (MQ=255)

                                                                                      ttAACACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAATTGATTGTCCATTTACTTAACCCACACttt      >  2:974195/1‑91 (MQ=255)

                                                                                      ttAACACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAATTGATTGTCCATTTACTTAACCCACACttt      >  2:543703/1‑91 (MQ=255)

                                                                                       tAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAATTGATTGTCCATTTACTTAACCCACACtttt     >  1:546790/1‑91 (MQ=255)

                                                                                       tAATACTGCTTCAATCTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCCATTGATTGTCCATTTACTTAACCCACACtttt     >  2:1896994/1‑91 (MQ=255)

                                                                                        aaTACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAATTGATTGTCTATTTACTTAATCCACACttttt    >  2:1913580/1‑91 (MQ=255)

                                                                                         aTACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAATTGATTGTCCATTTACTTAACCCACACTTTTTc   >  1:2790008/1‑91 (MQ=255)

                                                                                          tACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAATTGATTGTCCATTTACTTAGCCCACACTTTTTCt  <  2:3294343/91‑1 (MQ=255)

                                                                                          tACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAATTGATTGTCCATTTACTTAACCCACACTTTTTCt  <  1:928360/91‑1 (MQ=255)

                                                                                          tACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAATTGATTGTCCATTTACTTAACCCACACTTTTTCt  <  2:1933781/91‑1 (MQ=255)

CTTTATTTGAACTTCATCTAGTCCACCAGTTTGCTCTTTTCGATACCTCGCAATAAATGGTACTGTATTTTTTTCTTCTAGCAGTGTTAATACTGCTTCAATTTGTTTTTTACTAAATTGATATTTCTCTATGATTGAATTAATCAATTGATTGTCCATTTACTTAACCCACACTTTTTCT  >  USA300TCH1516_ALE/2184798‑2184978

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`