| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 2,211,673 | T→G | 5.8% | intergenic (+666/+147) | USA300HOU_RS11275 → / ← yidC | hypothetical protein/Membrane protein insertase YidC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 2,211,673 | 0 | T | G | 5.8% | 767.4 / 33.4 | 225 | intergenic (+666/+147) | USA300HOU_RS11275/yidC | hypothetical protein/Membrane protein insertase YidC |
| Reads supporting (aligned to +/- strand): ref base T (106/106); new base G (11/2); total (117/108) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.01e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01 | |||||||||||
Only 100 of 241 total aligned reads displayed.
AATGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCT > USA300TCH1516_ALE/2211583‑2211763 | aaTGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACAtt > 1:795904/1‑91 (MQ=255)aaTGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACAtt < 1:2274532/91‑1 (MQ=255) aTGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACAttt < 2:2918438/91‑1 (MQ=255) aTGACAACTCTGTTATTAACCACTTTTTACAAACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACAttt < 2:262976/91‑1 (MQ=255) tGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTg < 2:3462213/91‑1 (MQ=255) tGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTg > 1:48403/1‑91 (MQ=255) tGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTg > 1:675911/1‑91 (MQ=255) gACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGa > 2:609083/1‑91 (MQ=255) gACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGa < 2:1070280/91‑1 (MQ=255) gACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGa < 2:1070819/91‑1 (MQ=255) aCAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGaa < 1:1493203/91‑1 (MQ=255) cAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGaaa > 1:2811034/1‑91 (MQ=255) aaCTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAa‑t < 2:3431964/91‑1 (MQ=255) aCTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAa‑tc < 1:172054/91‑1 (MQ=255) ctctGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAa‑tca > 1:1368809/1‑91 (MQ=255) ctctGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAa‑tca > 1:3313986/1‑91 (MQ=255) gTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCtt < 1:901195/91‑1 (MQ=255) ttattaACTACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTa > 2:188402/1‑91 (MQ=255) ttattaACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACAtt > 1:1856659/1‑78 (MQ=255) ttattaACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACAtt < 2:1856659/78‑1 (MQ=255) ttattaACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTa > 2:1168306/1‑91 (MQ=255) ttattaACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTa > 2:1549981/1‑91 (MQ=255) ttattaACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTa < 2:2842016/91‑1 (MQ=255) tatttACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTAc < 1:3224853/91‑1 (MQ=255) attaACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTAct > 2:922140/1‑91 (MQ=255) taACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTActgc < 2:2934057/91‑1 (MQ=255) taACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTActgc > 1:131560/1‑91 (MQ=255) taACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTActgc < 2:1136553/91‑1 (MQ=255) aaCCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTActgct > 2:1801311/1‑91 (MQ=255) aCCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTActgctg < 2:3215517/91‑1 (MQ=255) aCCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTActgctg > 2:2561877/1‑91 (MQ=255) aCCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAAGATGA‑TTTCAAATGTGAAAGTCATCCTActgctg < 2:2888759/91‑1 (MQ=17) cACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGtt > 2:3119835/1‑91 (MQ=255) aCTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGttt < 1:1146779/91‑1 (MQ=255) aCTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGttt > 1:769527/1‑91 (MQ=255) aCTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGttt > 1:2103126/1‑91 (MQ=255) tttttACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCt < 1:3631466/91‑1 (MQ=255) aCATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTa < 1:2502989/91‑1 (MQ=255) aCATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTa > 1:2949276/1‑91 (MQ=255) aTACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATc < 2:2681417/91‑1 (MQ=255) aTACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATc > 2:916466/1‑91 (MQ=255) gAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACata > 1:1309593/1‑91 (MQ=255) aaaaCTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACatat < 1:3686026/91‑1 (MQ=255) aaaCTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATAtt < 2:2095322/91‑1 (MQ=255) aaCTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTg > 1:2049441/1‑91 (MQ=255) aCTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGt > 2:2544281/1‑91 (MQ=255) cTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCCCATATTGta > 1:3710387/1‑91 (MQ=255) cTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGta > 1:2688641/1‑91 (MQ=255) tCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGtat > 1:2674967/1‑91 (MQ=255) cGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGTCTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGtata < 2:1700718/91‑1 (MQ=255) cGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGtata < 2:2389856/91‑1 (MQ=255) tAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAAtg > 2:2342349/1‑91 (MQ=255) tAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAAtg > 2:277981/1‑91 (MQ=255) aaTATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAAtgt < 1:3633235/91‑1 (MQ=255) tattTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGacac < 2:585769/91‑1 (MQ=255) tattTCAAATAAAAACAGCAGTAGGATGACTCTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGacac > 2:2947812/1‑91 (MQ=255) attTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACt > 2:1302670/1‑91 (MQ=255) attTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACt > 1:3828715/1‑91 (MQ=255) attTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACt > 1:2302550/1‑91 (MQ=255) tttCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTa > 2:2804710/1‑91 (MQ=255) cAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGt < 2:1622575/91‑1 (MQ=255) cAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGt > 2:1651573/1‑91 (MQ=255) aaTAAAAACAGCAGTAGGATGCCTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGttt > 1:1418809/1‑91 (MQ=255) aaTAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGttt > 2:1759980/1‑91 (MQ=255) aTAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTc > 1:3490394/1‑91 (MQ=255) tAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCg < 1:1405335/91‑1 (MQ=255) agaaaCAGCAGTAAGATGA‑TTTCAAATGTGAAAGTCATCCTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGc > 1:1456074/3‑91 (MQ=14) aaCAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTAt < 2:1368809/91‑1 (MQ=255) aCAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCttca < 1:1262119/91‑4 (MQ=255) aCAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTAtt > 1:3712635/1‑91 (MQ=255) cagcagTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCttcaa < 1:1019678/91‑5 (MQ=255) cagcagTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTg > 1:719758/1‑91 (MQ=255) cagcagTAAGATGA‑TTTCAAATGTGAAAGTCATCCTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTg > 1:541050/1‑91 (MQ=17) agcagTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGa > 2:1534553/1‑91 (MQ=255) gcagTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGaa > 2:3635406/1‑91 (MQ=255) gcagTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGaa > 2:1991060/1‑91 (MQ=255) gcagTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGaa > 1:821075/1‑91 (MQ=255) cagTAAGATGA‑TTTCAAATGTGAAAGTCATCCTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAg > 1:969863/1‑91 (MQ=17) agTAAGATGA‑TTTCAAATGTGAAAGTCATCCTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGc > 1:193415/1‑91 (MQ=17) gTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCg < 1:623218/91‑1 (MQ=255) tAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGa > 1:3142535/1‑91 (MQ=255) aGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGaa < 1:930295/91‑1 (MQ=255) aGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGaa < 1:1614827/91‑1 (MQ=255) ggATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAA < 2:74347‑M1/91‑1 (MQ=255) gATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAA < 2:769527‑M1/91‑1 (MQ=255) gATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAA > 2:3310093‑M1/1‑91 (MQ=255) gATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAA > 2:374672‑M1/1‑91 (MQ=255) gATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAA > 1:2832324‑M1/1‑91 (MQ=255) aTGACTTCCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAA > 2:644147‑M1/1‑91 (MQ=255) tgatttcaaATGTGAAAGTCATCCTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAAT > 2:1067793‑M1/4‑91 (MQ=255) gACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATA < 2:3239185‑M1/91‑1 (MQ=255) cTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAAT < 2:2502546‑M1/91‑1 (MQ=255) cTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAAT < 1:187822‑M1/91‑1 (MQ=255) tCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTG > 2:1294429‑M1/1‑91 (MQ=255) cacaTTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGC > 2:3328595‑M1/1‑91 (MQ=255) acaTTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTgcg > 1:2454445/1‑91 (MQ=255) acaTTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTgcg > 2:2481947/1‑91 (MQ=255) aTTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGcc < 2:3516785/91‑1 (MQ=255) aTTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGcc > 1:1684599/1‑91 (MQ=255) ttGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCt > 1:2352478/1‑91 (MQ=255) | AATGACAACTCTGTTATTAACCACTTTTTACATACTGAAAACTCGTTAATATTATTTCAAATAAAAACAGCAGTAGGATGACTTTCACATTTGAAA‑TCATCTTACTGCTGTTTCTATTTATCACATATTGTATAATGTGACACTAAGTTTCGCTATTGAAGCGAAAAATAATGTGCGCCCT > USA300TCH1516_ALE/2211583‑2211763 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |