BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	126,542	A→G	5.7%	I119V (ATT→GTT)	lctP_1 →	L‑lactate permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	126,542	0	A	G	5.7%	631.0 / 15.5	196	I119V (ATT→GTT)	lctP_1	L‑lactate permease
Reads supporting (aligned to +/- strand): ref base A (86/98); new base G (8/4); total (94/102)
Fisher's exact test for biased strand distribution p-value = 2.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 205 total aligned reads displayed.

TATAAAGTTTCTATTAAAACAGGACAATTTTCTATTATTCAAGATAGTATTGCAAGTATTTCAGTGGACCAAAGAATCCAACTATTATTAATTGGATTTTGTTTCAACGCATTTTTAGAAGGTGCAGCAGGATTTGGTGTGCCAATTGCGATTTGTGCAGTATTATTAATTCAACTTGGAT  >  USA300TCH1516_ALE/126452‑126632

tataAAGTTTCTATTAAAACAGGACAATTTTCTATTATTCAAGATAGTATTGCAAGTATTTCAGTGGACCAAAGAATCCAACTATTATTaa                                                                                            <  2:1703576/91‑1 (MQ=255)

 ataAAGTTTCTATTAAAACAGGACAATTTTCTATTATTCAAGATAGTATTGCAAGTATTTCAGTGGACCAAAGAATCCAACTATTATTAAt                                                                                           <  2:2903728/91‑1 (MQ=255)

  taAAGTTTCTATTAAAACAGGACAATTTTCTATTATTCAAGATAGTATTGCAAGTATTTCAGTGGACCAAAGAATCCAACTATTATTAAtt                                                                                          <  1:2017571/91‑1 (MQ=255)

    aaGTTGCTATTAAAACAGGACAATTTTCTATTATTCAAGATAGTATTGCAAGTATTTCAGTGGACCAAAGAATCCAATTAATAATAGTTgg                                                                                        <  2:880700/91‑1 (MQ=255)

      gTTTCTATTAAAACAGGACAATTTTCTATTATTCAAGATAGTATTGCAAGTATTTCAGTGGACCGAAGAATCCAACTATTATTAATTGGAt                                                                                      <  2:2210692/91‑1 (MQ=255)

      gTTTCTATTAAAACAGGACAATTTTCTATTATTCAAGATAGTATTGCAAGTATTTCAGTGGACCAAAGAATCCAACTATTATTAATTGGAt                                                                                      >  1:2138994/1‑91 (MQ=255)

        ttCTATTAAAACAGGACAATTTTCTATTATTCAAGATAGTATTGCAAGTATTTCAGTTGACCAAAGAATCCAACTATTATTAATTGGAttt                                                                                    <  2:1204613/91‑1 (MQ=255)

        ttCTATTAAAACAGGACAATTTTCTATTATTCAAGATAGTATTGCAAGTATTTCAGTGGACCAAAGAATCCAACTATTATTAATTGGAttt                                                                                    >  2:1298048/1‑91 (MQ=255)

        ttCTATTAAAACAGGACAATTTTCTATTATTCAAGATAGTATTGCAAGTATTTCAGTGGACCAAAGAATCCAACTATTATTAATTGGAttt                                                                                    <  2:2128289/91‑1 (MQ=255)

        ttCTATTAAAACAGGACAATTTTCTATTATTCAAGATAGTATTGCAAGTATTTCAGTGGACCAAAGAATCCAACTATTATTAATTGGAttt                                                                                    >  1:882797/1‑91 (MQ=255)

        ttCTATTAAAACAGGACAATTTTCTATTATTCAAGATAGTATTGCAAGTATTTCAGTGGACCAAAGAATCCAACTATTATTAATTGGAttt                                                                                    <  1:368868/91‑1 (MQ=255)

         tCTATTAAAACAGGACAATTTTCTATTATTCAAGATAGTATTGCAAGTATTTCAGTGGACCAAAGAATCCAACTATTATTAATTGGAtttt                                                                                   <  2:2074764/91‑1 (MQ=255)

          cTATTAAAACAGGACAATTTTCTATTATTCAAGATAGTATTGCAAGTATTTCAGTGGACCAAAGAATCCAACTATTATTAATTGGATTTTg                                                                                  >  2:1014653/1‑91 (MQ=255)

          cTATTAAAACAGGACAATTTTCTATTATTCAAGATAGTATTGCAAGTATTTCAGTGGACCAAAGAATCCAACTATTATTAATTGGATTTTg                                                                                  <  1:3417171/91‑1 (MQ=255)

          cTATTAAAACAGGACAATTTTCTATTATTCAAGATAGTATTGCAAGTATTTCAGTGGACCAAAGAATCCAACTATTATTAATTGGATTTTg                                                                                  >  2:1887756/1‑91 (MQ=255)

          cTATTAAAACAGGACAATTTTCTATTATTCAAGATAGTATTGCAAGTATTTCAGTGGACCAAAGAATCCAACTATTATTAATTGGATTTTg                                                                                  <  1:1903450/91‑1 (MQ=255)

            aTTAAAACAGGACAATTTTCTATTATTCAAGATAGTATTGCAAGTATTTCAGTGGACCAAAGAATCCAACTATTATTAGTTGGATTTTGtt                                                                                <  1:2984985/91‑1 (MQ=255)

            aTTAAAACAGGACAATTTTCTATTATTCAAGATAGTATTGCAAGTATTTCAGTGGACCAAAGAATCCAACTATTATTAATTGGATTTTGtt                                                                                >  1:2825078/1‑91 (MQ=255)

               aaaaCAGGACAATTTTCTATTATTCAAGATAGTATTGCAGGTATTTCAGTGGACCAAAGAATCCAACTATTATTAATTGGATTTTGTTTCa                                                                             <  1:1412969/91‑1 (MQ=255)

                    aGGACAATTTTCTATTATTCAAGATAGTATTGCAAGTATTTCAGTGGACCAAAGAATCCAACTATTATTAATTGGATTTTGTTTCAACGCa                                                                        >  1:1862797/1‑91 (MQ=255)

                    aGGACAATTTTCTATTATTCAAGATAGTATTGCAAGTATTTCAGTGGACCAAAGAATCCAACTATTATTAATTGGATTTTGTTTCAACGCa                                                                        >  2:1368671/1‑91 (MQ=255)

                      gACAATTTTCTATTATTCAAGATAGTATTGCAAGTATTTCAGTGGACCAAAGAATCCAACTATTATTAATTGGATTTTGTTTCAACGCAtt                                                                      >  1:1444704/1‑91 (MQ=255)

                       aCAATTTTCTATTATTCAAGATAGTATTGCAAGTATTTCAGTGGACCAAAGAATCCAACTATTATTAATTGGATTTTGTTTCAACGCAttt                                                                     >  1:1026592/1‑91 (MQ=255)

                         aaTTTTCTATTATTCAAGATAGTATTGCAAGTATTTCAGTGGACCAAAGAATCCAACTATTATTAATTGGATTTTGTTTCAACGCAttttt                                                                   <  2:265519/91‑1 (MQ=255)

                         aaTTTTCTATTATTCAAGATAGTATTGCAAGTATTTCAGTGGACCAAAGAATCCAACTATTATTAATTGGATTTTGTTTCAACGCAttttt                                                                   >  2:1537470/1‑91 (MQ=255)

                         aaTTTTCTATTATTCAAGATAGTATTGCAAGTATTTCAGTGGACCAAAGAATCCAACTATTATTAATTGGATTTTGTTTCAACGCAttttt                                                                   <  2:617750/91‑1 (MQ=255)

                            tttCTATTATTCAAGATAGTATTGCAAGTATTTCAGTGGACCAAAGAATCCAACTATTATTAATTGGATTTTGTTTCAACGCATTTTTAGa                                                                <  2:1184491/91‑1 (MQ=255)

                             ttCTATTATTCAAGATAGTATTGCAAGTATTTCAGTGGACCAAAGAATCCAACTATTATTAATTGGATTTTGTTTCAACGCATTTTTAGaa                                                               <  1:3400359/91‑1 (MQ=255)

                              tCTATTATTCAAGATAGTATTGCAAGTATTTCAGTGGACCAAAGAATCCAACTATTATTAATTGGATTTTGTTTCAACGCATTTTTAGAAg                                                              <  1:1649476/91‑1 (MQ=255)

                              tCTATTATTCAAGATAGTATTGCAAGTATTTCAGTGGACCAAAGAATCCAACTATTATTAATTGGATTTTGTTTCAACGCATTTTTAGAAg                                                              <  1:2274793/91‑1 (MQ=255)

                               cTATTATTCAAGATAGTATTGCAAGTATTTCAGTGGACCAAAGAATCCAACTATTATTAATTGGATTTTGTTTCAACGCATTTTTAGAAgg                                                             >  2:3778610/1‑91 (MQ=255)

                                 attattCAAGATAGTATTGCAAGTATTTCAGTGGACCAAAGAATCCAACTATTATTAATTGGATTTTGTTTCAACGCATTTTTAGAAGGTg                                                           <  2:3265325/91‑1 (MQ=255)

                                  ttattCAAGATAGTATTGCAAGTATTTCAGTGGACCAAAGAATCCAACTATTATTAATTGGATTTTGTTTCAACGCATTTTTAGAAGGTgc                                                          <  1:2665472/91‑1 (MQ=255)

                                  ttattCAAGATAGTATTGCAAGTATTTCAGTGGACCAAAGAATCCAACTATTATTAATTGGATTTTGTTTCAACGCATTTTTAGAAGGTgc                                                          >  1:2850409/1‑91 (MQ=255)

                                  ttattCAAGATAGTATTGCAAGTATTTCAGTGGACCAAAGAATCCAACTATTATTAATTGGATTTTGTTTCAACGCATTTTTAGAAGGTgc                                                          <  2:3652611/91‑1 (MQ=255)

                                    attCAAGATAGTATTGCAAGTATTTCAGTGGACCAAAGAATCCAACTATTATTAATTGGATTTTGTTTCAACGCATTTTTAGAAGGTgcag                                                        <  1:1745697/91‑1 (MQ=255)

                                        aaGATAGTATTGCAAGTATTTCAGTGGACCAAAGAATCCAACTATTATTAATTGGATTTTGTTTCAACGCATTTTTAGAAGGTGCAGCAgg                                                    <  1:3837666/91‑1 (MQ=255)

                                         aGATACTATTGCAAGTATTTCAGTGGACCAAAGAATCCAACTATTATTAATTGGATTTTGTTTCAACGCATTTTTAGAAGGTGCAGCAGGa                                                   <  2:3137858/91‑1 (MQ=255)

                                          gATAGTATTGCAAGTATTTCAGTGGACCAAAGAATCCAACTATTATTAATTGGATTTTGTTTCAACGCATTTTTAGAAGGTGCAGCAGGAt                                                  <  1:245956/91‑1 (MQ=255)

                                          gATAGTAGTGCAAGTATTTCAGTGGACCAAAGAATCCAACTATTATTAATTGGATTTTGTTTCAACGCATTTTTAGAAGGTGCAGCAGGAt                                                  <  2:3451612/91‑1 (MQ=255)

                                           aTAGTATTGCAAGTATTTCAGTGGACCAAAGAATCCAACTATTATTAATTGGATTTTGTTTCAACGCATTTTTAGAAGGTGCAGCAGGAtt                                                 >  2:906826/1‑91 (MQ=255)

                                            tAGTATTGCAAGTATTTCAGTGGACCAAAGAATCCAACTATTATTAATTGGATTTTGTTTCAACGCATTTTTAGAAGGTGCAGCAGGAttt                                                >  1:484136/1‑91 (MQ=255)

                                             aGTATTGCAAGTATTTCAGTGGACCAAAGAATCCAACTATTATTAATTGGATTTTGTTTCAACGCATTTTTAGAAGGTGCAGCAGGATTTg                                               <  1:1386311/91‑1 (MQ=255)

                                                aTTGCAAGTATTTCAGTGGACCAAAGAATCCAACTATTATTAATTGGATTTTGTTTCAACGCATTTTTAGAAGGTGCAGCAGGATTTGgtg                                            >  1:2563215/1‑91 (MQ=255)

                                                aTTGCAAGTATTTCAGTGGACCAAAGAATCCAACTATTATTAATTGGATTTTGTTTCAACGCATTTTTAGAAGGTGCAGCAGGATTTGgtg                                            <  2:2405491/91‑1 (MQ=255)

                                                 ttGCAAGTATTTCAGTGGACCAAAGAATCCAACTATTATTAATTGGATTTTGTTTCAACGCATTTTTAGAAGGTGCAGCAGGATTTGgtgt                                           >  2:891291/1‑91 (MQ=255)

                                                 ttGCAAGTATTTCAGTGGACCAAAGAATCCAACTATTATTAATTGGATTTTGTTTCAACGCATTTTTAGAAGGTGCAGCAGGATTTGgtgt                                           >  1:3676976/1‑91 (MQ=255)

                                                 ttGCAAGTATTTCAGTGGACCAAAGAATCCAACTATTATTAATTGGATTTTGTTTCAACGCATTTTTAGAAGGTGCAGCAGGATTTGgtgt                                           <  1:3093683/91‑1 (MQ=255)

                                                 ttGCAAGTATTTCAGTGGACCAAAGAATCCAACTATTATTAATTGGATTTTGTTTCAACGCATTTTTAGAAGGTGCAGCAGGATTTGgtgt                                           >  2:239002/1‑91 (MQ=255)

                                                  tGCAAGTATTTCAGTGGACCAAAGAATCCAACTATTATTAATTGGATTTTGTTTCAACGCATTTTTAGAAGGTGCAGCAGGATTTGGtgtg                                          >  2:1276877/1‑91 (MQ=255)

                                                   gCAAGTATTTCAGTGGACCAAAGAATCCAACTATTATTAATTGGATTTTGTTTCAACGCATTTTTAGAAGGTGCAGCAGGATTTGGTGTGc                                         <  2:81899/91‑1 (MQ=255)

                                                     aaGTATTTCAGTGGACCAAAGAATCCAACTATTATTAATTGGATTTTGTTTCAACGCATTTTTAGAAGGTGCAGCAGGATTTGGTGTGCCa                                       <  2:2620751/91‑1 (MQ=255)

                                                     aaGTATTTCAGTGGACCAAAGAATCCAACTATTATTAATTGGATTTTGTTTCAACGCATTTTTAGAAGGTGCAGCAGGATTTGGTGTGCCa                                       <  1:1155560/91‑1 (MQ=255)

                                                     aaGTATTTCAGTGGACCAAAGAATCCAACTATTATTAATTGGATTTTGTTTCAACGCATTTTTAGAAGGTGCAGCAGGATTTGGTGTGCCa                                       >  1:2052905/1‑91 (MQ=255)

                                                     aaGTATTTCAGTGGACCAAAGAATCCAACTATTATTAATTGGATTTTGTTTCAACGCATTTTTAGAAGGTGCAGCAGGATTTGGTGTGCCa                                       >  1:2052577/1‑91 (MQ=255)

                                                      aGTATTTCAGTGGACCAAAGAATCCAACTATTATTAATTGGATTTTGTTTCAACGCATTTTTAGAAGGTGCAGCAGGATTTGGTGTGCCaa                                      >  1:905447/1‑91 (MQ=255)

                                                       gTATTTCAGTGGACCAAAGAATCCAACTATTATTAATTGGATTTTGTTTCAACGCATTTTTAGAAGGTGCAGCAGGATTTGGTGTGCCAAt                                     <  2:731860/91‑1 (MQ=255)

                                                         aTTTCAGTGGACCAAAGAATCCAACTATTATTAATTGGATTTTGTTTCAACGCATTTTTAGAAGGTGCAGCAGGATTTGGTGTGCCAATTg                                   <  2:541796/91‑1 (MQ=255)

                                                          tttCAGTGGACCAAAGAATCCAACTATTATTAATTGGATTTTGTTTCAACGCATTTTTAGAAGGTGCAGCAGGATTTGGTGTGCCAATTGc                                  >  2:1136189/1‑91 (MQ=255)

                                                          tttCAGTGGACCAAAGAATCCAACTATTATTAATTGGATTTTGTTTCAACGCATTTTTAGAAGGTGCAGCAGGATTTGGTGTGCCAATTGc                                  <  2:484136/91‑1 (MQ=255)

                                                          tttCAGTGGACCAAAGAATCCAACTATTATTAATTGGATTTTGTTTCAACGCATTTTTAGAAGGTGCAGCAGGATTTGGTGTGCCAATTGc                                  >  1:2267896/1‑91 (MQ=255)

                                                          tttCAGTGGACCAAAGAATCCAACTATTATTAATTGGATTTTGTTTCAACGCATTTTTAGAAGGTGCAGCAGGATTTGGTGTGCCAATTGc                                  >  2:3441706/1‑91 (MQ=255)

                                                           ttCAGTGGACCAAAGAATCCAGCTATTATTAATTGGATTTTGTTTCAACGCATTTTTAGAAGGTGCAGCAGGATTTGGTGTGCCAATTGCg                                 <  1:713034/91‑1 (MQ=255)

                                                           ttCAGTGGACCAAAGAATCCAACTATTATTAATTGGATTTTGTTTCAACGCATTTTTAGAAGGTGCAGCAGGATTTGGTGTGCCAATTGCg                                 <  2:2550349/91‑1 (MQ=255)

                                                            tCAGTGGACCAAAGAATCCAACTATTATTAATTGGATTTTGTTTCAACGCATTTTTAGAAGGTGCAGCAGGATTTGGTGTGCCAATTGCGa                                >  1:3506665/1‑91 (MQ=255)

                                                            tCAGTGGACCAAAGAATCCAACTATTATTAATTGGATTTTGTTTCAACGCATTTTTAGAAGGTGCAGCAGGATTTGGTGTGCCAATTGCGa                                <  1:3204106/91‑1 (MQ=255)

                                                             cAGTGGACCAAAGAATCCAACTATTATTAATTGGATTTTGTTTCAACGCATTTTTAGAAGGTGCAGCAGGATTTGGTGTGCCAATTGCGAt                               >  1:1593036/1‑91 (MQ=255)

                                                             cAGTGGACCAAAGAATCCAACTATTATTAATTGGATTTTGTTTCAACGCATTTTTAGAAGGTGCAGCAGGATTTGGTGTGCCAATTGCGAt                               >  2:3743545/1‑91 (MQ=255)

                                                              aGTGGACCAAAGAATCCAACTATTATTAATTGGATTTTGTTTCAACGCATTTTTAGAAGGTGCAGCAGGATTTGGTGTGCCAATTGCGAtt                              >  1:2800810/1‑91 (MQ=255)

                                                                tGGACCAAAGAATCCAACTATTATTAATTGGATTTTGTTTCAACGCATTTTTAGAAGGTGCAGCAGGATTTGGTGTGCCAATTGCGATTtg                            <  1:638544/91‑1 (MQ=255)

                                                                tGGACCAAAGAATCCAACTATTATTAATTGGATTTTGTTTCAACGCATTTTTAGAAGGTGCAGCAGGATTTGGTGTGCCAATTGCGATTtg                            >  1:2758967/1‑91 (MQ=255)

                                                                  gACCAAAGAATCCAACTATTATTAATTGGATTTTGTTTCAACGCATTTTTAGAAGGTGCAGCAGGATTTGGTGTGCCAATTGCGATTtgtg                          <  2:915684/91‑1 (MQ=255)

                                                                  gACCAAAGAATCCAACTATTATTAATTGGATTTTGTTTCAACGCATTTTTAGAAGGTGCAGCAGGATTTGGTGTGCCAATTGCGATTtgtg                          <  2:1431582/91‑1 (MQ=255)

                                                                   aCCAAAGAATCCAACTATTATTAATTGGATTTTGTTTCAACGCATTTTTAGAAGGTGCAGCAGGATTTGGTGTGCCAATTGCGATTTGTGc                         <  1:774250/91‑1 (MQ=255)

                                                                   aCCAAAGAATCCAACTATTATTAATTGGATTTTGTTTCAACGCATTTTTAGAAGGTGCAGCAGGATTTGGTGTGCCAATTGCGATTTGTGc                         >  2:1439503/1‑91 (MQ=255)

                                                                     cAAAGAATCCAACTATTATTAATTGGATTTTGTTTCAACGCATTTTTAGAAGGTGCAGCAGGATTTGGTGTGCCAATTGCGATTTGTGCAg                       <  1:3242430/91‑1 (MQ=255)

                                                                        aGAATCCAACTATTATTAATTGGATTTTGTTTCAACGCATTTTTAGAAGGTGCAGCAGGATTTGGTGTGCCAATTGCGATTTGTGCAGtat                    <  2:883238/91‑1 (MQ=255)

                                                                         aaaTCCAATTAATAATAGTTGGATTTTGTTTCAACGCATTTTTAGAAGGTGCAGCAGGATTTGGTGTGCCAATTGCGATTTGTGCAGtatt                   >  2:2851425/2‑91 (MQ=18)

                                                                         aaaTCCAATTAATAATAGTTGGATTTTGTTTCAACGCATTTTTAGAAGGTGCAGCAGGATTTGGTGTGCCAATTGCGATTTGTGCAGtatt                   >  1:57382/2‑91 (MQ=18)

                                                                          aaTCCAATTAATAATAGTTGGATTTTGTTTCAACGCATTTTTAGAAGGTGCAGCAGGATTTGGTGTGCCAATTGCGATTTGTGCAGtatta                  >  1:224891/1‑91 (MQ=18)

                                                                          aaTCCAACTATTATTAATTGGATTTTGTTTCAACGCATTTTTAGAAGGTGCAGCAGGATTTGGTGTGCCAATTGCGATTTGTGCAGtatta                  <  2:413640/91‑1 (MQ=255)

                                                                           aTCCAATTAATATTAGTTGGATTTTGTTTCAACGCATTTTTAGAAGGTGCAGCAGGATTTGGTGTGCCAATTGCGATTTGTGCAGtattat                 >  1:3000820/1‑91 (MQ=21)

                                                                           aTCCAACTGTTATTAATTGGATTTTGTTTCAACGCATTTTTAGAAGGTGCAGCAGGATTTGGTGTGCCAATTGCGATTTGTGCAGtattat                 >  1:2044353/1‑91 (MQ=255)

                                                                           aTCCAACTATTATTAATTGGATTTTGTTTCAACGCATTTTTAGAAGGTGCAGCAGGATTTGGTGTGCCAATTGCGATTTGTGCAGtattat                 <  1:423472/91‑1 (MQ=255)

                                                                            tCCAACTATTATTAATTGGATTTTGTTTCAACGCATTTTTAGAAGGTGCAGCAGGATTTGGTGTGCCAATTGCGATTTGTGCAGTattatt                <  1:1960468/91‑1 (MQ=255)

                                                                              cAACTATTATTAATTGGATTTTGTTTCAACGCATTTTTAGAAGGTGCAGCAGGATTTGGTGTGCCAATTGCGATTTGTGCAGTATTATTaa              <  2:2996426/91‑1 (MQ=255)

                                                                               aaCTATTATTAATTGGATTTTGTTTCAACGCATTTTTAGAAGGTGCAGCAGGATTTGGTGTGCCAATTGCGATTTGTGCAGTATTATTAAt             <  2:2267896/91‑1 (MQ=255)

                                                                                aCTATTATTAATTGGATTTTGTTTCAACGCATTTTTAGAAGGTGCAGCAGGATTTGGTGTGCCAATTGCGATTTGTGCAGTATTATTAAtt            >  2:2746292/1‑91 (MQ=255)

                                                                                aCTATTATTAATTGGATTTTGTTTCAACGCATTTTTAGAAGGTGCAGCAGGATTTGGTGTGCCAATTGCGATTTGTGCAGTATTATTAAtt            >  1:2455272/1‑91 (MQ=255)

                                                                                aCTATTATTAATTGGATTTTGTTTCAACGCATTTTTAGAAGGTGCAGCAGGATTTGGTGTGCCAATTGCGATTTGTGCAGTATTATTAAtt            >  2:2745904/1‑91 (MQ=255)

                                                                                 cTATTATTAATTGGATTTTGTTTCAACGCATTTTTAGAAGGTGCAGCAGGATTTGGTGTGCCAATTGCGATTTGTGCAGTATTATTAATTc           >  1:2082953/1‑91 (MQ=255)

                                                                                  tattatTAATTGGATTTTGTTTCAACGCATTTTTAGAAGGTGCAGCAGGATTTGGTGTGCCAATTGCGATTTGTGCAGTATTATTAATTCa          <  2:3357383/91‑1 (MQ=255)

                                                                                     tattaATTGGATTTTGTTTCAACGCATTTTTAGAAGGTGCAGCAGGATTTGGTGTGCCAATTGCGATTTGTGCAGTATTATTAATTCAACt       >  1:2865850/1‑91 (MQ=255)

                                                                                      attaATTGGATTTTGTTTCAACGCATTTTTAGAAGGTGCAGCAGGATTTGGTGTGCCAATTGCGATTTGTGCAGTATTATTAATTCAACtt      <  2:2634344/91‑1 (MQ=255)

                                                                                      attaATTGGATTTTGTTTCAACGCATTTTTAGAAGGTGCAGCAGGATTTGGTGTGCCAATTGCGATTTGTGCAGTATTATTAATTCAACtt      >  1:782811/1‑91 (MQ=255)

                                                                                       ttaATTGGATTTTGTTTCAACGCATTTTTAGAAGGTGCAGCAGGATTTGGTGTGCCAATTGCGATTTGTGCAGTATTATTAATTCAACTTg     <  1:2600283/91‑1 (MQ=255)

                                                                                         aaTTGGATTTTGTTTCAACGCATTTTTAGAAGGTGCAGCAGGATTTGGTGTGCCAATTGCGATTTGTGCAGTATTATTAATTCAACTTGGa   <  2:3548475/91‑1 (MQ=255)

                                                                                          aTTGGATTTTGTTTCAACGCATTTTTAGAAGGTGCAGCAGGATTTGGTGTGCCAATTGCGATTTGTGCAGTATTATTAATTCAACTTGGAt  <  2:772020/91‑1 (MQ=255)

                                                                                          aTTGGATTTTGTTTCAACGCATTTTTAGAAGGTGCAGCAGGATTTGGTGTGCCAATTGCGATTTGTGCAGTATTATTAATTCAACTTGGAt  <  1:3655801/91‑1 (MQ=255)

                                                                                          aTTGGATTTTGTTTCAACGCATTTTTAGAAGGTGCAGCAGGATTTGGTGTGCCAATTGCGATTTGTGCAGTATTATTAATTCAACTTGGAt  >  1:2575744/1‑91 (MQ=255)

TATAAAGTTTCTATTAAAACAGGACAATTTTCTATTATTCAAGATAGTATTGCAAGTATTTCAGTGGACCAAAGAATCCAACTATTATTAATTGGATTTTGTTTCAACGCATTTTTAGAAGGTGCAGCAGGATTTGGTGTGCCAATTGCGATTTGTGCAGTATTATTAATTCAACTTGGAT  >  USA300TCH1516_ALE/126452‑126632

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`