| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 2,566,052 | A→G | 7.6% | intergenic (+101/+39) | nhaK_2 → / ← plaP | Sodium, potassium, lithium and rubidium/H(+) antiporter/Low‑affinity putrescine importer PlaP |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 2,566,052 | 0 | A | G | 7.6% | 788.1 / 38.3 | 253 | intergenic (+101/+39) | nhaK_2/plaP | Sodium, potassium, lithium and rubidium/H(+) antiporter/Low‑affinity putrescine importer PlaP |
| Reads supporting (aligned to +/- strand): ref base A (131/100); new base G (8/11); total (141/112) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.38e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01 | |||||||||||
Only 100 of 265 total aligned reads displayed.
ACAATACCCCCGTACATATGAGACAAAGTCATTATCATCTCATATGTACGGGGTTTTTATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGTTAACATTCTTTTGATA > USA300TCH1516_ALE/2565962‑2566141 | aCAATACCCCCGTACATATGAGACAAAGTCATTATCATCTCATATGTACGGGGTTTTTATATTCAACATCAAAAAATCAGATTGATGaaaa > 2:1339266/1‑91 (MQ=255)aCAATACCCCCGTACATATGAGACAAAGTCATTATCATCTCATATGTACGGGGTTTTTATATTCAACATCAAAAAATCAGATTGATGaaaa > 2:11928/1‑91 (MQ=255) cAATACCCCCGTACATATGAGACAAAGTCATTATCATCTCATATGTACGGGGTTTTTATATTCAACATCAAAAAATCAGATTGATGAAAAg > 1:1620911/1‑91 (MQ=255) aTACCCCCGTACATATGAGACAAAGTCATTATCATCTCATATGTACGGGGTTTTTATATTCAACATCAAAAAATCAGATTGATGAAAAGTa < 1:1073423/91‑1 (MQ=255) tACCCCCGTACATATGAGACAAAGTCATTATCATCTCATATGTACGGGGTTTTTATATTCAACATCAAAAAATCAGATTGATGAAAAGTaa < 2:657521/91‑1 (MQ=255) tACCCCCGTACATATGAGACAAAGTCATTATCATCTCATATGTACGGGGTTTTTATATTCAACATCAAAAAATCAGATTGATGAAAAGTaa < 1:209679/91‑1 (MQ=255) cccccGTACATATGAGACAAAGTCATTATCATCTCATATGTACGGGGTTTTTATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAAt > 2:2292992/1‑91 (MQ=255) cccccGTACATATGAGACAAAGTCATTATCATCTCATATGTACGGGGTTTTTATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAAt < 2:2485515/91‑1 (MQ=255) ccccGTACATATGATACAAAGTCATTATCATCTCATATGTACGGGGTTTTTATAATCAGCAACAAAAAATCAGATTGATGAAAAGTAAATa < 2:3803850/91‑1 (MQ=255) ccccGTACATATGAGACAAAGTCATTATCATCTCATATGTACGGGGTTTTTATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAATa < 1:1138223/91‑1 (MQ=255) ccccGTACATATGAGACAAAGTCATTATCATCTCATATGTACGGGGTTTTTATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAATa < 1:832065/91‑1 (MQ=255) cccGTACATATGAGACAAAGTCATTATCATCTCATATGTACGGGGTTTTTATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAATaa > 1:183837/1‑91 (MQ=255) gTACATATGAGACAAAGTCATTATCATCTCATATGTACGGGGTTTTTATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCt < 2:3642380/91‑1 (MQ=255) tatGAGACAAAGTCATTATCATCTCATATGTACGGGGTTTTTATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTtcat > 1:2588005/1‑91 (MQ=255) atGAGACAAAGTCATTATCATCTCATATGTACGGGGTTTTTATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTtcatc < 1:3792032/91‑1 (MQ=255) tGAGACAAAGTCATTATCATCTCATATGTACGGGGTTTTTATATACAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTtcatca < 2:1546796/91‑1 (MQ=255) gagaCAAAGTCATTATCATCTCATATGTACGGGGTTTTTATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCaa > 2:2215128/1‑91 (MQ=255) agaCAAAGTCATTATCATCTCATATGTACGGGGTTTTTATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAAt < 1:2077304/91‑1 (MQ=255) agaCAAAGTCATTATCATCTCATATGTACGGGGTTTTTATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAAt < 1:86814/91‑1 (MQ=255) cAAAGTCATTATCATCTCATATGTACGGGGTTTTTATATTCAACATCAAAAAATCAGATTGATGAAAGGTTATTTACTTTTCATCAATctg < 1:1464697/91‑3 (MQ=21) aaaGTCATTATCATCTCATATGTACGGGGTTTTTATATTCAACATCAAAAAATCGGATTGATGAAAGGTTATTTACTTTTCATCAATctga < 2:1716861/91‑4 (MQ=18) aGTCATTATCATCTCATATGTACGGGGTTTTTATATTCAACATCAAAAAATCGGATTGATGAAAGGTTATTTACTTTTCATCAATCTGAtt < 1:2708078/91‑1 (MQ=17) aGTCATTATCATCTCATATGTACGGGGTTTTTATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGAtt > 1:3594731/1‑91 (MQ=255) catgatcatcTCATATGTACGGGGTTTTTATATTCAACATCAAAAAATCGGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGa < 2:1065076/87‑1 (MQ=255) cATTATCATCTCATATGTACGGGGTTTTTATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGa > 2:2316204/1‑91 (MQ=255) cATTATCATCTCATATGTACGGGGTTTTTATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGa > 2:2082184/1‑91 (MQ=255) aTTATCATCTCATATGTACGGGGTTTTTATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGAt > 1:1593245/1‑91 (MQ=255) aTTATCATCTCATATGTACGGGGTTTTTATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGAt > 2:3292026/1‑91 (MQ=255) ttATCATCTCATATGTACGGGGTTTTTATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGAtt > 1:2711322/1‑91 (MQ=255) tttgtctcATATGTACGGGGTTTTTATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATtat > 1:3019547/5‑91 (MQ=255) tttgtctcATATGTACGGGGTTTTTATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATtat > 2:2596962/5‑91 (MQ=255) atcatcTCATATGTACGGGGTTTTTATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATtat > 2:2819111/1‑91 (MQ=255) atcatcTCATATGTACGGGGTTTTTATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATtat > 2:2755801/1‑91 (MQ=255) tcatcTCATATGTACGGGGTTTTTATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATtata < 2:3303636/91‑1 (MQ=255) tcatcTCATATGTACGGGGTTTTTATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATtata < 2:1679935/91‑1 (MQ=255) catcTCATATGTACGGGGTTTTTATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAg < 2:3123153/91‑1 (MQ=255) atcTCATATGTACGGGGTTTTTATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGa > 1:614641/1‑91 (MQ=255) ctcATATGTACGGGGTTTTTATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAAt < 2:1409453/91‑1 (MQ=255) ctcATATGTACGGGGTTTTTATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAAt > 2:3494955/1‑91 (MQ=255) tcATATGTACGGGGTTTTTATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCATTCCGATTTGATTATAGAATc < 1:1518361/91‑1 (MQ=255) tcATATGTACGGGGTTTTTATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATc < 2:3346369/91‑1 (MQ=255) cATATGTACGGGGTTTTTATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCt < 1:2916765/91‑1 (MQ=255) atGTACGGGGTTTTTATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTAtt < 2:2130823/91‑1 (MQ=255) atGTACGGGGTTTTTATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTAtt < 2:3555645/91‑1 (MQ=255) atGTACGGGGTTTTTATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTAtt > 1:2695288/1‑91 (MQ=255) tACGGGGTTTTTATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTAttttt > 2:1359881/1‑91 (MQ=255) aCGGGGTTTTTATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTAtttttt < 2:1420836/91‑1 (MQ=255) gggTTTTTATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAg > 1:2171769/1‑91 (MQ=255) gggTTTTTATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAg > 2:3167152/1‑91 (MQ=255) ggTTTTTATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTCTTTAAGt > 1:1948520/1‑91 (MQ=255) gTTTTTATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGtt < 2:2637433/91‑1 (MQ=255) gTTTTTATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGtt > 1:3794740/1‑91 (MQ=255) ttttATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTa > 1:2658824/1‑91 (MQ=255) ttttATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTa < 1:3716826/91‑1 (MQ=255) tttATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTaa > 1:2349645/1‑91 (MQ=255) ttATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTaaa > 2:3850965/1‑91 (MQ=255) ttATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTaaa > 1:1699073/1‑91 (MQ=255) tataTTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAAt > 2:1768591/1‑91 (MQ=255) tataTTCAACATCAAAAAATCAGAGTGATGAAAAGTAAATAACCTTTCATCAATCCGACTTGATTACAGAATCTATTTTTTAAGTTTAAAt > 2:1769151/1‑91 (MQ=255) atatTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATg < 1:1376793/91‑1 (MQ=255) tatTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATgg < 2:1856376/91‑1 (MQ=255) ttCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAATTGGaa > 2:1075931/1‑91 (MQ=255) ttCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGaa > 2:2433843/1‑91 (MQ=255) tCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAAt < 2:1909663/91‑1 (MQ=255) cAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAAtt > 1:2600149/1‑91 (MQ=255) aCATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGt < 1:3420835/91‑1 (MQ=255) cATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTa > 1:2481538/1‑91 (MQ=255) tCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACa > 1:1173179/1‑91 (MQ=255) tCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACa > 1:1911245/1‑91 (MQ=255) atcaaaTCGGATTGATGAAAGGTTATTTACTTTTCATCAATCTGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATa > 2:3790133/4‑91 (MQ=17) aaaaaaTCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGCTTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATa > 1:2791423/1‑91 (MQ=255) aaaaaaTCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATa > 1:2137365/1‑91 (MQ=255) aaaTCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGt > 2:1728474/1‑91 (MQ=255) aaTCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGtt < 2:2695288/91‑1 (MQ=255) aaTCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGtt < 1:2936436/91‑1 (MQ=255) aTCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGTTa < 1:3293735/91‑1 (MQ=255) tCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGTTaa > 1:2276642/1‑91 (MQ=255) tCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGTTaa > 2:874909/1‑91 (MQ=255) tCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGTTaa < 1:503077/91‑1 (MQ=255) cAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGTTAAc < 2:502732/91‑1 (MQ=255) ggATTGATGAAAGGTTATTTACTTTTCATCAATCTGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGTTAACa > 2:241101/2‑91 (MQ=18) aGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTg < 1:3010962/77‑1 (MQ=255) aGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGTTAACa < 1:417257/91‑1 (MQ=255) gATTGATGAAAGGTTATTTACTTTTCATCAATCTGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGTTAACAt > 1:757460/1‑91 (MQ=21) gATTGATGAAAGGTTATTTACTTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTTAGTTTATTTGGAATTGTACATACGTTAACAt > 2:322679/1‑91 (MQ=18) gATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGTTAACAt < 2:2831828/91‑1 (MQ=255) gATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATGTTTTAAGTTTAAATGGAATTGTACATACGTTAACAt > 2:3060321/1‑91 (MQ=255) gATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATACAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGTTAACAt > 1:3076767/1‑91 (MQ=255) aTTGATGAAAGGTTATTTACTTTTCATCAATCTGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGTTAACAtt > 2:2026564/1‑91 (MQ=25) aTTGATGAAAGGTTATTTACTTTTCATCAATCTGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGTTAACAtt > 2:1281082/1‑91 (MQ=21) aTTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGTTAACAtt > 2:3741048/1‑91 (MQ=255) aTTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGTTAACAtt > 2:972544/1‑91 (MQ=255) ttGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGTTAACATTc > 1:1006692/1‑91 (MQ=255) ttGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGTTAACATTc < 2:2973885/91‑1 (MQ=255) tgatgaAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGTTAACATTCt > 1:3214511/1‑91 (MQ=255) gatgaAACGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGTTAACATTCtt < 2:3451189/91‑1 (MQ=255) gatgaAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGTTAACATTCtt < 2:366732/91‑1 (MQ=255) tgaAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGTTAACATTCtttt < 1:261072/91‑1 (MQ=255) aaaaGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGTTAACATTCTTTTGa < 1:988306/91‑1 (MQ=255) aaGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGTTAACATTCTTTTGAta < 1:1424781/91‑1 (MQ=255) | ACAATACCCCCGTACATATGAGACAAAGTCATTATCATCTCATATGTACGGGGTTTTTATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGTTAACATTCTTTTGATA > USA300TCH1516_ALE/2565962‑2566141 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |