Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 2,566,074 C→T 5.5% intergenic (+123/+17) nhaK_2 → / ← plaP Sodium, potassium, lithium and rubidium/H(+) antiporter/Low‑affinity putrescine importer PlaP

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,566,0740CT 5.5% 887.6 / 24.3 269intergenic (+123/+17)nhaK_2/plaPSodium, potassium, lithium and rubidium/H(+) antiporter/Low‑affinity putrescine importer PlaP
Reads supporting (aligned to +/- strand):  ref base C (158/96);  new base T (8/7);  total (166/103)
Fisher's exact test for biased strand distribution p-value = 5.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.90e-01

Only 100 of 274 total aligned reads displayed.

AAAGTCATTATCATCTCATATGTACGGGGTTTTTATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGTTAACATTCTTTTGATAAATTAAATACAATTTCATACGC  >  USA300TCH1516_ALE/2565986‑2566163
                                                                                        |                                                                                         
  aGTCATTATCATCTCATATGTACGGGGTTTTTATATTCAACATCAAAAAATCGGATTGATGAAAGGTTATTTACTTTTCATCAATCTGAtt                                                                                       <  1:2708077/91‑1 (MQ=17)
   gTCATTATCATCTCATATGTACGGGGTTTTTATATTCAACATCAAAAAATCGGATTGATGAAAGGTTATTTACTTTTCATCAATCTGAttt                                                                                      <  2:2411516/91‑1 (MQ=17)
   gTCATTATCATCTCATATGTACGGGGTTTTTATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGAttt                                                                                      >  2:2937663/1‑91 (MQ=255)
     cATTATCATCTCATATGTACGGGGTTTTTATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGa                                                                                    >  2:2316204/1‑91 (MQ=255)
     cATTATCATCTCATATGTACGGGGTTTTTATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGa                                                                                    >  2:2082184/1‑91 (MQ=255)
     cATTATCATCTCATATGTACGGGGTTTTTATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGa                                                                                    <  2:473513/91‑1 (MQ=255)
     cATTATCATCTCATATGTACGGGGTTTTTATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGa                                                                                    >  2:3664397/1‑91 (MQ=255)
      aTTATCATCTCATATGTACGGGGTTTTTATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGAt                                                                                   >  2:824679/1‑91 (MQ=255)
       ttATCATCTCATATGTACGGGGTTTTTATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGAtt                                                                                  >  1:2711235/1‑91 (MQ=255)
       ttATCATCTCATATGTACGGGGTTTTTATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGAtt                                                                                  <  1:941419/91‑1 (MQ=255)
         tttgtctcATATGTACGGGGTTTTTATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATtat                                                                                >  2:2596962/5‑91 (MQ=255)
         atcatcTCATATGTACGGGGTTTTTATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATtat                                                                                <  2:1365094/91‑1 (MQ=255)
         atcatcTCATATGTACGGGGTTTTTATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATtat                                                                                <  2:1061179/91‑1 (MQ=255)
         atcatcTCATATGTACGGGGTTTTTATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATtat                                                                                <  1:2395041/91‑1 (MQ=255)
          tcatcTCATATGTACGGGGTTTTTATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATtata                                                                               <  2:1679935/91‑1 (MQ=255)
           catcTCATATGTACGGGGTTTTTATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAg                                                                              <  2:356734/91‑1 (MQ=255)
              ctcATATGTACGGGGTTTTTATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAAt                                                                           >  2:3429517/1‑91 (MQ=255)
              ctcATATGTACGGGGTTTTTATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAAt                                                                           >  2:3494955/1‑91 (MQ=255)
              ctcATATGTACGGGGTTTTTATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAAt                                                                           <  1:657526/91‑1 (MQ=255)
               tcATATGTACGGGGTTTTTATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATc                                                                          <  2:3346369/91‑1 (MQ=255)
                cATATGTACGGGGTTTTTATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCt                                                                         <  1:2917588/91‑1 (MQ=255)
                       aCGGGGTTTTTATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTAtttttt                                                                  <  2:1420836/91‑1 (MQ=255)
                          gggTTTTTATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAg                                                               >  1:2171769/1‑91 (MQ=255)
                          gggTTTTTATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAg                                                               <  1:500544/91‑1 (MQ=255)
                            gTTTTTATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGtt                                                             <  2:2637433/91‑1 (MQ=255)
                                ttATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTaaa                                                         >  2:1724446/1‑91 (MQ=255)
                                ttATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTCaaa                                                         >  1:3125846/1‑91 (MQ=255)
                                 tatcTTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAAt                                                        >  2:1768543/1‑91 (MQ=255)
                                 tataTTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACTTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAAt                                                        <  2:1614008/91‑1 (MQ=255)
                                 tataTTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAAt                                                        >  2:1813858/1‑91 (MQ=255)
                                 tataTTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAAt                                                        >  1:1698094/1‑91 (MQ=255)
                                 tataTTCAACATCAAAAAATCAGAGTGATGAAAAGTAAATAACCTTTCATCAATCCGACTTGATTACAGAATCTATTTTTTAAGTTTAAAt                                                        >  2:1769151/1‑91 (MQ=255)
                                  atatTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATg                                                       <  1:1867166/91‑1 (MQ=255)
                                  atatTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATg                                                       >  1:379320/1‑91 (MQ=255)
                                     ttCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAATTGGaa                                                    >  2:1075931/1‑91 (MQ=255)
                                      tCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAAt                                                   <  1:1917080/91‑1 (MQ=255)
                                      tCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAAt                                                   <  2:1909663/91‑1 (MQ=255)
                                        aaCATCAAAAAATCAGATTGATGAAACGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAACTGGAATTg                                                 >  1:808378/1‑91 (MQ=255)
                                        aaCATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTg                                                 >  1:2619365/1‑91 (MQ=255)
                                        aaCATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTg                                                 <  1:160692/91‑1 (MQ=255)
                                         aCATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGt                                                >  2:3293735/1‑91 (MQ=255)
                                         aCATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGt                                                >  1:457400/1‑91 (MQ=255)
                                          cATCAAAAAATCAGATTGATGAAAAGTAAATAACCCTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTa                                               >  1:2481639/1‑91 (MQ=255)
                                           aTCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTAc                                              >  2:1348107/1‑91 (MQ=255)
                                            tCAAAGAATCAGATTGATGAAAAGTAAATAACCTTTCATGAATCCGATTTGATGATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACa                                             <  1:3605731/91‑1 (MQ=255)
                                            tCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACa                                             <  2:3163423/91‑1 (MQ=255)
                                            tCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACa                                             >  1:1173179/1‑91 (MQ=255)
                                             cAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACAt                                            >  1:1370568/1‑91 (MQ=255)
                                              atcaaaTCGGATTGATGAAAGGTTATTTACTTTTCATCAATCTGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATa                                           >  2:3790133/4‑91 (MQ=17)
                                              aaaaaaTCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATa                                           >  1:2137365/1‑91 (MQ=255)
                                                 aaaTCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGt                                        <  1:2294837/91‑1 (MQ=255)
                                                 aaaTCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGt                                        >  2:1205993/1‑91 (MQ=255)
                                                  aaTCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGtt                                       >  1:3250762/1‑91 (MQ=255)
                                                  aaTCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGtt                                       <  1:2937663/91‑1 (MQ=255)
                                                    tCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGTTaa                                     >  1:2668693/1‑91 (MQ=255)
                                                      aGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTg                                                 >  2:3010962/1‑77 (MQ=255)
                                                       gATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGTTAACAt                                  <  2:2831650/91‑1 (MQ=255)
                                                       gATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGTTAACAt                                  >  1:272804/1‑91 (MQ=255)
                                                       gATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGTTAACAt                                  >  2:2714511/1‑91 (MQ=255)
                                                       gATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATGTTTTAAGTTTAAATGGAATTGTACATACGTTAACAt                                  >  2:3060321/1‑91 (MQ=255)
                                                        aTTGATGAAAGGTTATTTACTTTTCATCAATCTGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGTTAACAtt                                 >  2:2026564/1‑91 (MQ=25)
                                                        aTTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGTTAACAtt                                 >  2:1250996/1‑91 (MQ=255)
                                                        aTTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGTTAACAtt                                 >  2:3741048/1‑91 (MQ=255)
                                                        aTTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGTTAACAtt                                 >  2:972544/1‑91 (MQ=255)
                                                        aTTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTATTAAGTTTAAATGGAATTGTACATACGTTAACAtt                                 >  2:3278321/1‑91 (MQ=255)
                                                         ttGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGTTAACATTc                                >  1:1309806/1‑91 (MQ=255)
                                                         ttGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGTTAACATTc                                >  1:1006692/1‑91 (MQ=255)
                                                           gatgaAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGTTAACATTCtt                              >  1:3826744/1‑91 (MQ=255)
                                                            atgaAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGTTAACATTCttt                             <  1:1420018/91‑1 (MQ=255)
                                                             tgaAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGTTAACATTCtttt                            <  1:261072/91‑1 (MQ=255)
                                                             tgaAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGTTAACATTCtttt                            <  2:3214511/91‑1 (MQ=255)
                                                               aaaaGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGTTAACATTCTTTTGa                          <  1:180927/91‑1 (MQ=255)
                                                                 aaGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGTTAACATTCTTTTGAta                        >  1:1069221/1‑91 (MQ=255)
                                                                   gTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGTTAACATTCTTTTGAtaaa                      >  1:1561962/1‑91 (MQ=255)
                                                                    tAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGTTAACATTCTTTTGAtaaat                     >  1:2259049/1‑91 (MQ=255)
                                                                    tAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGTTAACATTCTTTTGAtaaat                     <  1:54328/91‑1 (MQ=255)
                                                                     aaaTAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGTTAACATTCCTTTGAtaaatt                    >  1:3511920/1‑91 (MQ=255)
                                                                      aaTAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGTTAACATTCTTTTGAtaaatta                   <  1:2063954/91‑1 (MQ=255)
                                                                      aaTAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGTTAACATTCTTTTGAtaaatta                   >  1:2454268/1‑91 (MQ=255)
                                                                       aTAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGTTAACATTCTTTTGAtaaattca                  >  2:2778540/1‑89 (MQ=255)
                                                                       aTAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGTTAACATTCTTTTGAtaaattaa                  >  2:2418034/1‑91 (MQ=255)
                                                                       aTAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGTTAACATTCTTTTGAtaaattaa                  >  1:690301/1‑91 (MQ=255)
                                                                        tAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGTTAACATTCTTTTGAtaaattaaa                 >  1:3663500/1‑91 (MQ=255)
                                                                         aaCCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGTTAACATTCTTTTGAtaaattaaat                >  2:533244/1‑91 (MQ=255)
                                                                         aaCCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGTTAACATTCTTTTGAtaaattaaat                >  2:1818189/1‑91 (MQ=255)
                                                                           ccTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGTTAACATTCTTTTGATAAATTAAATAc              >  2:1551656/1‑91 (MQ=255)
                                                                           ccTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGTTAACATTCTTTTGATAAATTAAATAc              >  1:3443882/1‑91 (MQ=255)
                                                                           ccTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGTTAACATTCTTTTGATAAATTAAATAc              >  2:3453592/1‑91 (MQ=255)
                                                                             tttCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGTTAACATTCTTTTGAtaaatta                   >  1:3016336/1‑84 (MQ=255)
                                                                                catcaATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGTTAACATTCttt                             >  1:1729217/1‑71 (MQ=255)
                                                                                catcaATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGTTAACATTCttt                             <  2:1729217/71‑1 (MQ=255)
                                                                                catcaATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGTTAACATTCTTTTGATAAATTAAATACAAttt         <  1:3366702/91‑1 (MQ=255)
                                                                                  tcaATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGTTAACATTCTTTTGATAAATTAAATACAATTTCa       >  1:1489886/1‑91 (MQ=255)
                                                                                  tcaATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGTTAACATTCTTTTGATAAATTAAATACAATTTCa       <  2:204731/91‑1 (MQ=255)
                                                                                  tcaATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGTTAACATTCTTTTGATAAATTAAATACAATTTCa       <  2:3125846/91‑1 (MQ=255)
                                                                                   caATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGTTAACATTCTTTTGATAAATTAAATACAATTTCAt      >  2:2265837/1‑91 (MQ=255)
                                                                                     aTCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGTTAACATTCTTTTGATAAATTAAATACAATTTCATAc    >  2:1010936/1‑91 (MQ=255)
                                                                                      tCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGTTAACATTCTTTTGATAAATTAAATACAATTTCCTACg   <  1:3061054/91‑1 (MQ=255)
                                                                                      tCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGTTAACATTCTTTTGATAAATTAAATACAATTTCATACg   <  1:2819111/91‑1 (MQ=255)
                                                                                       ccGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGTTAACATTCTTTTGATAAATTAAATACAATTTCATACGc  <  1:613711/91‑1 (MQ=255)
                                                                                        |                                                                                         
AAAGTCATTATCATCTCATATGTACGGGGTTTTTATATTCAACATCAAAAAATCAGATTGATGAAAAGTAAATAACCTTTCATCAATCCGATTTGATTATAGAATCTATTTTTTAAGTTTAAATGGAATTGTACATACGTTAACATTCTTTTGATAAATTAAATACAATTTCATACGC  >  USA300TCH1516_ALE/2565986‑2566163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑