BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	2,669,453	C→T	5.5%	A118V (GCT→GTT)	yicL →	putative inner membrane transporter YicL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,669,453	0	C	T	5.5%	599.4 / 13.7	180	A118V (GCT→GTT)	yicL	putative inner membrane transporter YicL
Reads supporting (aligned to +/- strand): ref base C (88/82); new base T (3/7); total (91/89)
Fisher's exact test for biased strand distribution p-value = 2.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 196 total aligned reads displayed.

GACGTATGTTAGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTG  >  USA300TCH1516_ALE/2669363‑2669542

gACGTATGTTAGGCAAATTACTTATGTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAAtgc                                                                                           <  1:3599663/91‑1 (MQ=255)

 aCGTATGTTAGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAAtgct                                                                                          >  2:1492645/1‑91 (MQ=255)

  cGTATGTTAGGCAAATTACTTATCTTCAGTATACTGGGCATGTTCGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAAtgctg                                                                                         >  2:890980/1‑91 (MQ=255)

  cGTATGTTAGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAAtgctg                                                                                         >  1:2296698/1‑91 (MQ=255)

  cGTATGTTAGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAAtgctg                                                                                         >  2:3789983/1‑91 (MQ=255)

     aTGTTAGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGa                                                                                      <  1:285067/91‑1 (MQ=255)

          aGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGcg                                                                                 >  2:3849830/1‑90 (MQ=255)

          aGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCa                                                                                 <  1:2153318/91‑1 (MQ=255)

          aGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCa                                                                                 <  1:1357850/91‑1 (MQ=255)

          aGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAATAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCa                                                                                 >  1:3276362/1‑91 (MQ=255)

            gCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAAc                                                                               >  1:1177325/1‑91 (MQ=255)

             cAAATTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGTTGCAATCGCagca                                                                              <  2:3257732/91‑4 (MQ=255)

              aaaTTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAAtgc                                                                                           >  2:1910178/1‑77 (MQ=255)

              aaaTTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACAt                                                                             >  2:746459/1‑91 (MQ=255)

                  tACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGTTGCAATCGCAGCATtacc                                                                         <  1:2305764/91‑2 (MQ=38)

                   aCTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTACTGCTGCGATTGCAACATtacta                                                                        >  2:2948877/1‑91 (MQ=255)

                   aCTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGTTGCAATCGCAGCATtacct                                                                        <  2:642546/91‑3 (MQ=38)

                   aCTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGTTGCAATCGCAGCATtacct                                                                        <  2:1267928/91‑3 (MQ=38)

                   aCTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATtacta                                                                        >  2:3622674/1‑91 (MQ=255)

                   aCTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATtacta                                                                        >  1:1987418/1‑91 (MQ=255)

                    cTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATtactac                                                                       <  1:2192093/91‑1 (MQ=255)

                     ttATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACa                                                                      >  2:3708784/1‑91 (MQ=255)

                      tATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACaa                                                                     <  1:1443423/91‑1 (MQ=255)

                       aTCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAAt                                                                    >  1:3647041/1‑91 (MQ=255)

                         cTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATAc                                                                  <  2:1623944/91‑1 (MQ=255)

                         cTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATAc                                                                  <  1:3045524/91‑1 (MQ=255)

                            cAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACAtt                                                               <  1:2891884/91‑1 (MQ=255)

                              gTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTgc                                                             <  2:941139/91‑1 (MQ=255)

                               tataCTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTgcg                                                            <  1:271523/91‑1 (MQ=255)

                                ataCTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCAATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTgcgc                                                           >  1:3362291/1‑91 (MQ=255)

                                ataCTGAGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTacgc                                                           >  1:3012532/1‑91 (MQ=255)

                                 taCTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGcc                                                          >  2:2500270/1‑91 (MQ=255)

                                 taCTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGcc                                                          >  1:1698023/1‑91 (MQ=255)

                                 taCTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGcc                                                          >  2:2611230/1‑91 (MQ=255)

                                   cTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAg                                                        >  2:241570/1‑91 (MQ=255)

                                    tGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGt                                                       >  1:1060619/1‑91 (MQ=255)

                                     gggCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGtt                                                      >  2:2286344/1‑91 (MQ=255)

                                     gggCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGtt                                                      <  1:1605752/91‑1 (MQ=255)

                                       gCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTta                                                    >  2:354455/1‑91 (MQ=255)

                                        cATGTTCGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGCTTGCAACATTACTACAATACATCGCGCCAGTTtat                                                   >  2:1333746/1‑91 (MQ=255)

                                        cATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTtat                                                   >  2:823702/1‑91 (MQ=255)

                                        cATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTtat                                                   >  2:2061020/1‑91 (MQ=255)

                                           gTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGTCAGTTTAtatt                                                >  1:3517295/1‑91 (MQ=255)

                                             tagtagTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTTCAACATTACTACAATACATTGCGCCAGTTTAtattat                                              <  1:3273384/91‑1 (MQ=255)

                                              agtagtACATTATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATGGCGCCAGTTTAtattatt                                             <  2:723558/91‑1 (MQ=255)

                                              agtagtACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTAtattatt                                             <  2:3318490/91‑1 (MQ=255)

                                                tggtaCTATGTGCTTATTTGGCATCTTTTAATGCGGGTGATGCTGGGTTTGCAACTTTATTAGAATACTTTGCGCCAGTTTAtattatttt                                           <  2:1997113/89‑3 (MQ=255)

                                                tagtaCAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTAtattattat                                           <  2:2154010/91‑1 (MQ=255)

                                                tagtaCAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTAtattattat                                           >  2:41544/1‑91 (MQ=255)

                                                 agtaCAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATattattatt                                          <  1:2529354/91‑1 (MQ=255)

                                                  gtaCAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTAttt                                         >  1:398671/1‑91 (MQ=255)

                                                  gtaCAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTAttt                                         <  2:2440748/91‑1 (MQ=255)

                                                  gtaCAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTAttt                                         <  2:1789511/91‑1 (MQ=255)

                                                   taCAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTg                                        <  2:2166090/91‑1 (MQ=255)

                                                       atatGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGttt                                    >  1:3783418/1‑91 (MQ=255)

                                                         atGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGt                                  >  1:2192670/1‑91 (MQ=255)

                                                          tGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTc                                 <  1:1889602/91‑1 (MQ=255)

                                                            cTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCAt                               >  2:2992198/1‑91 (MQ=255)

                                                             ttATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATa                              <  2:2308275/91‑1 (MQ=255)

                                                              tataTGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATaa                             >  2:1599663/1‑91 (MQ=255)

                                                                  tGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATaa                             >  2:1068750/1‑87 (MQ=255)

                                                                  tGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATaa                             <  1:1068750/87‑1 (MQ=255)

                                                                  tGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAgg                         >  2:2247591/1‑91 (MQ=255)

                                                                   ggCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGc                        <  2:3480233/91‑1 (MQ=255)

                                                                   ggCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGc                        <  1:315580/91‑1 (MQ=255)

                                                                   ggCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGc                        <  2:2714470/91‑1 (MQ=255)

                                                                    gCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCg                       <  2:908218/91‑1 (MQ=255)

                                                                    gCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCg                       <  1:3071449/91‑1 (MQ=255)

                                                                     cATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGTGGCGt                      >  2:3148371/1‑91 (MQ=255)

                                                                      aTCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGtt                     >  1:1591094/1‑91 (MQ=255)

                                                                      aTCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGtt                     <  2:237458/91‑1 (MQ=255)

                                                                       tCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTg                    >  1:680975/1‑91 (MQ=255)

                                                                          aTTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCaa                 <  1:2359079/91‑1 (MQ=255)

                                                                           ttAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCaaa                >  2:3530113/1‑91 (MQ=255)

                                                                           ttAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCaaa                >  1:847431/1‑91 (MQ=255)

                                                                           ttAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCaaa                >  1:587864/1‑91 (MQ=255)

                                                                            tAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCaaaa               <  1:2423030/91‑1 (MQ=255)

                                                                            tAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCaaaa               >  1:3679593/1‑91 (MQ=255)

                                                                            tAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCaaaa               >  1:883678/1‑91 (MQ=255)

                                                                              aTACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACt             <  2:586348/91‑1 (MQ=255)

                                                                               tACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTa            >  2:1523496/1‑91 (MQ=255)

                                                                               tACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTa            <  2:2191689/91‑1 (MQ=255)

                                                                               tACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTa            <  1:168224/91‑1 (MQ=255)

                                                                                aCAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTaa           <  2:2877228/91‑1 (MQ=255)

                                                                                aCAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTaa           <  1:1148278/91‑1 (MQ=255)

                                                                                aCAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTaa           <  2:2877054/91‑1 (MQ=255)

                                                                                 gtagTAATGTTGCAATCGCAGCATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAAc          >  1:3633357/4‑91 (MQ=37)

                                                                                  tagTAATGTTGCAATCGCAGCATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACa         >  1:750467/3‑91 (MQ=38)

                                                                                  tagTAATGTTGCAATCGCAGCATTACTACAATACATTGCGCCAGTTTATATTATTATTGGGTTTGTCATAAGAGGCGTTGCAAAACTAACa         >  1:3295583/3‑91 (MQ=37)

                                                                                  aGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACa         <  2:2231079/91‑1 (MQ=255)

                                                                                   ggTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACat        <  1:2679632/91‑1 (MQ=255)

                                                                                   ggTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACat        >  2:1584288/1‑91 (MQ=255)

                                                                                   ggTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACat        <  1:89672/91‑1 (MQ=255)

                                                                                    gTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACatt       >  1:279862/1‑91 (MQ=255)

                                                                                      aaTGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTAGGTTTGTCATAAGAGGCGTTGCAAAACTAACattag     >  2:3834305/1‑90 (MQ=255)

                                                                                       aTGCTGCGATTGCAACATTACTACGATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACattatt    >  2:484276/1‑91 (MQ=255)

                                                                                       aTGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACattatt    >  2:259850/1‑91 (MQ=255)

                                                                                       aTGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACattatt    >  1:2285078/1‑91 (MQ=255)

                                                                                        tgctgcGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTAttt   >  1:1021908/1‑91 (MQ=255)

                                                                                         gctgcGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTACCATTATTTg  <  1:1947550/91‑1 (MQ=255)

GACGTATGTTAGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTG  >  USA300TCH1516_ALE/2669363‑2669542

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`