BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	2,669,457	G→A	5.4%	A119A (GCG→GCA)	yicL →	putative inner membrane transporter YicL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,669,457	0	G	A	5.4%	651.0 / 14.3	197	A119A (GCG→GCA)	yicL	putative inner membrane transporter YicL
Reads supporting (aligned to +/- strand): ref base G (97/89); new base A (4/7); total (101/96)
Fisher's exact test for biased strand distribution p-value = 3.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 200 total aligned reads displayed.

ATGTTAGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTG  >  USA300TCH1516_ALE/2669368‑2669547

     aGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGcg                                                                                      >  2:3849830/1‑90 (MQ=255)

     aGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCa                                                                                      <  1:2153318/91‑1 (MQ=255)

     aGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCa                                                                                      >  1:1216335/1‑91 (MQ=255)

     aGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCa                                                                                      <  1:1357850/91‑1 (MQ=255)

        cAAATTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACa                                                                                   <  1:2448416/91‑1 (MQ=255)

             tACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGTTGCAATCGCAGCATtacc                                                                              <  1:2305383/91‑2 (MQ=38)

              aCTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTACTGCTGCGATTGCAACATtacta                                                                             >  2:2948877/1‑91 (MQ=255)

              aCTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGTTGCAATCGCAGCATtacct                                                                             <  2:642546/91‑3 (MQ=38)

              aCTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATtacta                                                                             >  1:3425824/1‑91 (MQ=255)

              aCTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATtacta                                                                             >  2:3545559/1‑91 (MQ=255)

               cTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATtactac                                                                            <  1:2192093/91‑1 (MQ=255)

                ttATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGTTGCAATCGCAGCATtacctgt                                                                           <  1:2550276/91‑5 (MQ=37)

                ttATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACa                                                                           >  2:707888/1‑91 (MQ=255)

                ttATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACa                                                                           >  2:3708784/1‑91 (MQ=255)

                 tATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACaa                                                                          <  1:1443423/91‑1 (MQ=255)

                  aTCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAAt                                                                         >  1:3647041/1‑91 (MQ=255)

                    cTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATAc                                                                       <  2:1623944/91‑1 (MQ=255)

                    cTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATAc                                                                       <  1:3045577/91‑1 (MQ=255)

                     ttCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACa                                                                      <  2:3309371/91‑1 (MQ=255)

                      tCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACAt                                                                     <  1:1071530/91‑1 (MQ=255)

                         gTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTgc                                                                  <  2:941139/91‑1 (MQ=255)

                         gTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACACTgc                                                                  >  1:3122436/1‑91 (MQ=255)

                          tataCTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTgcg                                                                 <  1:271523/91‑1 (MQ=255)

                           ataCTGAGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTacgc                                                                >  1:3011507/1‑91 (MQ=255)

                           ataCTGAGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTacgc                                                                >  1:3012532/1‑91 (MQ=255)

                            taCTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGcc                                                               >  1:2100382/1‑91 (MQ=255)

                            taCTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGcc                                                               <  1:3287752/91‑1 (MQ=255)

                            taCTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGcc                                                               >  2:2611230/1‑91 (MQ=255)

                            taCTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGcc                                                               >  2:2500270/1‑91 (MQ=255)

                            taCTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGcc                                                               >  1:1698023/1‑91 (MQ=255)

                              cTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAg                                                             >  2:241570/1‑91 (MQ=255)

                                gggCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGtt                                                           <  1:1605752/91‑1 (MQ=255)

                                gggCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCAGCGATTGCAACATTACTACAATACATTGCGCCAGtt                                                           >  2:3506436/1‑91 (MQ=255)

                                   cATGTTCGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGCTTGCAACATTACTACAATACATCGCGCCAGTTtat                                                        >  2:1333746/1‑91 (MQ=255)

                                   cATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTtat                                                        >  2:2061020/1‑91 (MQ=255)

                                       ttAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTAtatta                                                    <  1:948130/91‑1 (MQ=255)

                                        tagtagTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTAtattat                                                   >  1:357451/1‑91 (MQ=255)

                                        tagtagTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACACTACTACAATACATTGCGCCAGTTTAtattat                                                   <  1:3273467/91‑1 (MQ=255)

                                         agtagtACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTAtattatt                                                  >  1:3349043/1‑91 (MQ=255)

                                         agtagtACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTAtattatt                                                  <  2:740255/91‑1 (MQ=255)

                                          gtagtaCAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTAtattatta                                                 >  1:2880468/1‑91 (MQ=255)

                                           tagtaCAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTAtattattat                                                >  2:41544/1‑91 (MQ=255)

                                           tagtaCAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTAtattattat                                                <  2:2154010/91‑1 (MQ=255)

                                            agtaCAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATattattatt                                               <  2:1177325/91‑1 (MQ=255)

                                             gtaCAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTAttt                                              <  2:1789511/91‑1 (MQ=255)

                                             gtaCAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTAttt                                              <  2:2440748/91‑1 (MQ=255)

                                             gtaCAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTAttt                                              >  1:398671/1‑91 (MQ=255)

                                              taCAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTg                                             >  1:1063981/1‑91 (MQ=255)

                                              taCAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTg                                             <  2:2166090/91‑1 (MQ=255)

                                              taCAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTg                                             <  2:814067/91‑1 (MQ=255)

                                                  atatGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGttt                                         >  1:3783418/1‑91 (MQ=255)

                                                    atGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGt                                       >  1:2192670/1‑91 (MQ=255)

                                                     tGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTc                                      <  1:1889602/91‑1 (MQ=255)

                                                       cTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCAt                                    >  2:2992198/1‑91 (MQ=255)

                                                        ttATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATa                                   <  2:2308274/91‑1 (MQ=255)

                                                        ttATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATa                                   >  1:1155813/1‑91 (MQ=255)

                                                         tataTGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATaa                                  >  2:1599663/1‑91 (MQ=255)

                                                           tatGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAaga                                >  2:3638606/1‑91 (MQ=255)

                                                            atGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAagag                               <  1:3194673/91‑1 (MQ=255)

                                                             tGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAgg                              <  2:3534650/91‑1 (MQ=255)

                                                             tGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAgg                              >  2:2247591/1‑91 (MQ=255)

                                                               gCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCg                            <  2:908218/91‑1 (MQ=255)

                                                                cATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGTGGCGt                           >  2:3148371/1‑91 (MQ=255)

                                                                cATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGt                           <  2:3353109/91‑1 (MQ=255)

                                                                 aTCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGtt                          <  2:237458/91‑1 (MQ=255)

                                                                 aTCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGtt                          >  1:1591094/1‑91 (MQ=255)

                                                                  tCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTg                         >  1:680975/1‑91 (MQ=255)

                                                                     aTTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCaa                      <  1:2359006/91‑1 (MQ=255)

                                                                      ttAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCaaa                     >  1:847431/1‑91 (MQ=255)

                                                                      ttAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCaaa                     >  1:587864/1‑91 (MQ=255)

                                                                       tAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCaaaa                    <  1:2422949/91‑1 (MQ=255)

                                                                       tAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCaaaa                    >  1:3679593/1‑91 (MQ=255)

                                                                       tAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCaaaa                    >  1:883678/1‑91 (MQ=255)

                                                                        aaTACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAAc                   >  2:1971308/1‑91 (MQ=255)

                                                                         aTACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACt                  <  2:586348/91‑1 (MQ=255)

                                                                          tACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTa                 <  1:168224/91‑1 (MQ=255)

                                                                          tACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTa                 <  2:2191689/91‑1 (MQ=255)

                                                                          tACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTa                 >  2:1523496/1‑91 (MQ=255)

                                                                           aCAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTaa                <  2:2877054/91‑1 (MQ=255)

                                                                            gtagTAATGTTGCAATCGCAGCATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAAc               >  1:3633357/4‑91 (MQ=37)

                                                                             tagTAATGTTGCAATCGCAGCATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACa              >  1:750467/3‑91 (MQ=38)

                                                                             aGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACa              <  2:2231079/91‑1 (MQ=255)

                                                                              ggTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACat             <  1:2679632/91‑1 (MQ=255)

                                                                              ggTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACat             <  1:89672/91‑1 (MQ=255)

                                                                              ggTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACat             >  2:1584288/1‑91 (MQ=255)

                                                                               gTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACatt            >  1:279862/1‑91 (MQ=255)

                                                                                 aaTGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACattat          <  2:2933659/91‑1 (MQ=255)

                                                                                 aaTGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACattat          <  2:868550/91‑1 (MQ=255)

                                                                                 aaTGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTAGGTTTGTCATAAGAGGCGTTGCAAAACTAACattag          >  2:3834305/1‑90 (MQ=255)

                                                                                  atgttgcGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACattatt         >  2:2284699/5‑91 (MQ=255)

                                                                                  atgttgcAATCGCAGCATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCACAACTAACactatt         >  2:2724265/5‑91 (MQ=255)

                                                                                  aTGCTGCGATTGCAACATTACTACGATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACattatt         >  2:484276/1‑91 (MQ=255)

                                                                                  aTGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACattatt         >  1:2285078/1‑91 (MQ=255)

                                                                                  aTGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACattatt         >  2:259850/1‑91 (MQ=255)

                                                                                   tgctgcGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTAttt        >  1:1021908/1‑91 (MQ=255)

                                                                                    gctgcGAGTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAGCATTATTTg       <  1:1947594/91‑1 (MQ=255)

                                                                                      tgcGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGAt     <  2:1702171/91‑1 (MQ=255)

                                                                                      tgcGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGAt     <  2:3200328/91‑1 (MQ=255)

                                                                                        cGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGAtgt   >  1:2745054/1‑91 (MQ=255)

                                                                                         gATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGAtgtg  >  2:2323748/1‑91 (MQ=255)

ATGTTAGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTG  >  USA300TCH1516_ALE/2669368‑2669547

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`