| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 2,833,387 | G→C | 10.3% | intergenic (‑723/+367) | lipA_2 ← / ← hisI | Lipase 1/Phosphoribosyl‑AMP cyclohydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 2,833,387 | 0 | G | C | 10.3% | 702.3 / 56.2 | 214 | intergenic (‑723/+367) | lipA_2/hisI | Lipase 1/Phosphoribosyl‑AMP cyclohydrolase |
| Reads supporting (aligned to +/- strand): ref base G (94/98); new base C (7/15); total (101/113) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.76e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
Only 100 of 217 total aligned reads displayed.
TTCGGGATGCATATCTTAGTTCTAATGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATTTATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTCGCCATTTTTTCCCTT > USA300TCH1516_ALE/2833298‑2833477 | tCGGGATGCATATCTTAGTTCTAATGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATTTATAGta < 2:2309513/91‑1 (MQ=255) cGGGATGCATATCTTAGTTCTAATGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATTTATAGtat > 1:154034/1‑91 (MQ=255) cGGGATGCATATCTGAGTTCTAAGGGAGAGTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATAGATTTGTATTTATACtat < 2:1279766/91‑1 (MQ=255) cGGGATACATATCTTAGTTCTAATGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATTTATAGtat > 1:3018850/1‑91 (MQ=255) gggATGCATATCTTAGTTCTAATGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATTTATAGtata > 1:512825/1‑91 (MQ=255) ggATGCATATCTTAGTTCTAATGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATTTATAGTATaa > 1:372049/1‑91 (MQ=255) ggATGCATATCTTAGTTCTAATGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATTTATAGTATaa > 1:94845/1‑91 (MQ=255) ggATGCATATCTTAGTTCTAATGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATTTATAGTATaa > 2:1814387/1‑91 (MQ=255) gATGCATATCTTAGTTCTAATGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATTTATAGTATaaa < 1:409554/91‑1 (MQ=255) gATGCATATCTTAGTTCTAATGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATTTATAGTATaaa < 1:2710396/91‑1 (MQ=255) aTGCATATCTTAGTTCTAATGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTCCATATATTTGCATTTATAGTATAAAt > 2:3364933/1‑91 (MQ=255) aTGCATATCTTAGTTCTAATGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATTTATAGTATAAAt < 2:813352/91‑1 (MQ=255) aTGCATATCTTAGTTCTAATGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATTTATAGTATAAAt > 2:3810880/1‑91 (MQ=255) aTGCAGATCTTAGTTCTAATGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATTTATAGTATAAAt < 2:3574677/91‑1 (MQ=255) gCATATCTTAGTTCTAATGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATTTATAGTATAAATAc < 1:3585223/91‑1 (MQ=255) gCATATCTTAGTTCTAATGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATTTATACTATAAATAc < 1:1105097/91‑1 (MQ=255) cATATCTTAGTTCTAATGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATTTATACTATAAATACa < 1:2012216/91‑1 (MQ=255) atatCTTAGTTCTAATGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATTTATACTATAAATACaa < 1:3181123/91‑1 (MQ=255) tatCTTAGTTCTAATGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATTTATAGTATAAATACaaa < 2:1598809/91‑1 (MQ=255) tatCTTAGTTCTAATGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATTTATAGTATAAATACaaa > 1:2426532/1‑91 (MQ=255) tatCTTAGTTCTAATGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATTTATAGTATAAATACaaa < 2:536005/91‑1 (MQ=255) tatCTTAGTTCTAATGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATTTATACTATAAATACaaa < 2:752220/91‑1 (MQ=255) atCTTGGTTCTGATGGAGGTTGTCTTTTAAATAGGCTATGACATTACTATTTCAGGCTAGATATATTTGTAGTTATACTATAAATACaaat < 2:3857729/91‑2 (MQ=255) tAGTTCTAATGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATTTATAGTATAAATACAAAGAATg < 1:1425758/91‑1 (MQ=255) gTTCTAATGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATTTATAGTATAAATACAAAGAATGTa < 1:755162/91‑1 (MQ=255) gTTCTAATGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATTTATAGTATAAATACAAAGAATGTa > 2:651014/1‑91 (MQ=255) ttCTAATGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATTTATAGTATAAATACAAAGAATGTaa < 2:875597/91‑1 (MQ=255) cTAATGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATTTATAGTATAAATACAAAGAATGTaaaa > 2:3848000/1‑90 (MQ=255) cTAATGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATTTATAGTATAAATACAAAGAATGTAAAg < 2:2969796/91‑1 (MQ=255) cTAATGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATTTATAGTATAAATACAAAGAATGTAAAg < 1:764952/91‑1 (MQ=255) aTGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATTTATAGTATAAATACAAAGAATGTAAAGACa > 2:986556/1‑91 (MQ=255) gagaTTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATTTATAGTATAAATACAAAGAATGTAAAGACAACt > 1:1222247/1‑91 (MQ=255) gagaTTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATTTATAGTATAAATACAAAGAATGTAAAGACAACt > 1:993526/1‑91 (MQ=255) gagaTTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATTTATAGTATAAATACAAAGAATGTAAAGACAACt < 1:1264871/91‑1 (MQ=255) aTTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATTTATAGTATAAATACAAAGAATGTAAAGACAACTATg > 1:461418/1‑91 (MQ=255) aTTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATTTATAGTATAAATACAAAGAATGTAAAGACAACTATg < 2:2701928/91‑1 (MQ=255) aTTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATTTATAGTATAAATACAAAGAATGTAAAGACAACTATg > 1:238890/1‑91 (MQ=255) ttGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATTTATAGTATAAATACAAAGAATGTAAAGACAACTATGa > 1:1924251/1‑91 (MQ=255) tGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATTTATAGTATAAATACAAAGAATGTAAAGACAACTATGat < 2:1319745/91‑1 (MQ=255) gTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATTTATAGTATAAATACAAAGAATGTAAAGACAACTATGata > 1:2019292/1‑91 (MQ=255) tCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATTTATAGTATAAATACAAAGAATGTAAAGACAACTATGatat > 2:60427/1‑91 (MQ=255) tCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATTTATAGTATAAATACAAAGAATGTAAAGACAACTATGatat > 2:392569/1‑91 (MQ=255) cTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATTTATAGTATAAATACAAAGAATGTAAAGACAACTATGATAtt < 2:238890/91‑1 (MQ=255) ttttAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATTTATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTa > 2:3508210/1‑91 (MQ=255) ttAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATTTATAGTGTAAATACAAAGAATGTAAAGACAACTATGATATTAAt < 2:1479754/91‑1 (MQ=255) aaTAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATTTATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAgg < 2:438936/91‑1 (MQ=255) aaTAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATTTATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAgg < 2:2284501/91‑1 (MQ=255) gCTATGACATTTCTATTTCAGGCTAGATATATTTGTATTTATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGgtagta < 1:3473241/91‑1 (MQ=255) gCTATGACATTTCTATTTCAGGCTAGATATATTTGTATTTATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGgtagta < 1:3771246/91‑1 (MQ=255) cTATGACATTTCTATTTCAGGCTAGATATATTTGTATTTATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTaa > 1:1710213/1‑91 (MQ=255) tGACATTTCTATTTCAGGCTAGATATATTTGTATTTATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGa < 1:1577416/91‑1 (MQ=255) tGACATTTCTATTTCAGGCTAGATATATTTGTATTTATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGa > 1:194685/1‑91 (MQ=255) gACATTTCTATTTCAGGCTAGATATATTTGTATTTATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGAc > 2:91448/1‑91 (MQ=255) aCATTTCTATTTCAGGCTAGATATATTTGTATTTATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACa < 2:1797146/91‑1 (MQ=255) aCATTTCTATTTCAGGCTAGATATATTTGTATTTATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACa < 2:3747689/91‑1 (MQ=255) cATTTCTATTTCAGGCTAGATATATTTGTATTTATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACaa > 1:2239284/1‑91 (MQ=255) tCTATTTCAGGCTAGATATATTTGTATTTATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACAt < 2:1231885/91‑1 (MQ=255) tATTTCAGGCTAGATATATTTGTATTTATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATaa > 1:2440039/1‑91 (MQ=255) tttCAGGCTAGATATATTTGTATTTATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAAct > 1:3042307/1‑91 (MQ=255) ttCAGGCTAGATATATTTGTATTTATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAActc < 2:1935993/91‑1 (MQ=255) ttCAGGCTAGATATATTTGTATTTATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAActc < 2:1936178/91‑1 (MQ=255) ttCAGGCTAGATATATTTGTATTTATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAActc < 2:3771105/91‑1 (MQ=255) cAGGCTAGATATATTTGTATTTATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCtt < 1:3145488/91‑1 (MQ=255) aGGCTAGATATATTTGTATTTATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCttt < 2:1691246/91‑1 (MQ=255) ggCTAGATATATTTGTATTTATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTa > 2:1989472/1‑91 (MQ=255) ggCTAGATATATTTGTATTTATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTa > 2:3234901/1‑91 (MQ=255) cTAGATATATTTGTATTTATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGc > 2:3482802/1‑91 (MQ=255) cTAGATATATTTGTATTTATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGc < 1:3399448/91‑1 (MQ=255) tAGATATATTTGTATTTATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCt < 2:1924251/91‑1 (MQ=255) gATATATTTGTATTTATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCttt > 1:1079899/1‑91 (MQ=255) gATATATTTGTATTTATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCttt < 2:3165403/91‑1 (MQ=255) gATATATTTGTATTTATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCttt > 2:2806859/1‑91 (MQ=255) atatatTTGTATTTATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCtttt > 1:2835492/1‑91 (MQ=255) atatatTTGTATTTATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCtttt < 1:131779/91‑1 (MQ=255) atatatTTGTATTTATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCtttt < 1:23339/91‑1 (MQ=255) tatatTTGTATTTATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCttttt < 2:2838924/91‑1 (MQ=255) tatatTTGTATTTATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCttttt < 2:3284563/91‑1 (MQ=255) tatatTTGTATTTATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCtattt > 1:2344605/1‑91 (MQ=255) tatTTGTATTTATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTCg < 1:3535189/91‑1 (MQ=255) atTTGTATTTATAGTATAAATACAAAGAATGTAGAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTCGc < 1:120606/91‑1 (MQ=255) atTTGTATTTATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTCGc > 1:2150046/1‑91 (MQ=255) atTTGTATTTATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTCGc > 1:3850229/1‑91 (MQ=255) tttGTATTTATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTCGcc < 2:2298398/91‑1 (MQ=255) tttGTATTTATACTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTCGcc > 2:2353498/1‑91 (MQ=255) ttGTATTTATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTCGCCa < 1:3633526/91‑1 (MQ=255) tGTATTTATACTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTCGCCAt > 2:1411219/1‑91 (MQ=255) gTATTTATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTCGCCAtt < 2:223248/91‑1 (MQ=255) gTATTTATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTCGCCAtt > 2:1653999/1‑91 (MQ=255) tttATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTCGCCAttttt > 1:686702/1‑91 (MQ=255) tttATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTCGCCAttttt < 2:920282/91‑1 (MQ=255) tttATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTCGCCAttttt > 1:805388/1‑91 (MQ=255) tttATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTCGCCAttttt > 2:2010453/1‑91 (MQ=255) tttATACTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTCGCCAttttt > 1:3342536/1‑91 (MQ=255) ttATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTCGCCAtttttt > 2:2977384/1‑91 (MQ=255) ataGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTCGCCATTTTTTcc > 2:563331/1‑91 (MQ=255) ataGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTCGCCATTTTTTcc > 1:1653215/1‑91 (MQ=255) ataGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTCGCCATTTTTTcc > 1:185036/1‑91 (MQ=255) aGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTCGCCATTTTTTCCCt < 1:2352105/91‑1 (MQ=255) gTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTCGCCATTTTTTCCCtt < 2:1542832/91‑1 (MQ=255) gTAAAAACACAAAGAAAGTAAAGACAACTTTGATCTTAATAAGTCATAACGACAACCATGACTCTTTAGCTTTTTCGCCATTTTTGCCCtt < 2:2617070/91‑1 (MQ=255) | TTCGGGATGCATATCTTAGTTCTAATGGAGATTGTCTTTTAAATAGGCTATGACATTTCTATTTCAGGCTAGATATATTTGTATTTATAGTATAAATACAAAGAATGTAAAGACAACTATGATATTAATAGGTAGTAACGACAAACATAACTCTTTAGCTTTTTCGCCATTTTTTCCCTT > USA300TCH1516_ALE/2833298‑2833477 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |