| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 253,991 | A→C | 7.4% | intergenic (+204/+158) | asbF → / ← USA300HOU_RS01125 | 3‑dehydroshikimate dehydratase/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 253,991 | 0 | A | C | 7.4% | 742.8 / 37.5 | 227 | intergenic (+204/+158) | asbF/USA300HOU_RS01125 | 3‑dehydroshikimate dehydratase/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (126/84); new base C (9/8); total (135/92) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.13e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 231 total aligned reads displayed.
GTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATAT > USA300TCH1516_ALE/253902‑254080 | tttgtAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATAACCATTCACAGTAACAATCCTCACCATTTAAAAGAGTATATAAcc < 1:3837431/91‑1 (MQ=255) ttgtAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCt > 2:1387571/1‑91 (MQ=255) ttgtAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCt < 1:296192/91‑1 (MQ=255) tgtAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCtt < 2:2894416/91‑1 (MQ=255) gtAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCttt > 2:20883/1‑91 (MQ=255) gtAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCttt > 1:1703507/1‑91 (MQ=255) gtAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCttt > 1:169661/1‑91 (MQ=255) gtAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCttt > 1:1438027/1‑91 (MQ=255) tAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCtttt < 1:2833612/91‑1 (MQ=255) aaaGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACTATTGAAAAGGTTATATACTCTTTTc < 1:1020057/91‑1 (MQ=37) aaaGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTc > 1:13176/1‑91 (MQ=255) aaaGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTc > 1:13012/1‑91 (MQ=255) aaaGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTc > 1:2630029/1‑91 (MQ=255) agaatGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACTATTGAAAAGGTTATATACTCTTTTCaa < 1:1332870/91‑1 (MQ=25) agaagGTGTACTTTATATAAGTATATAGCGATGGTCGTACCCATTCACACTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCaa < 1:3355507/91‑1 (MQ=255) gaagGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACTATTGAAAAGGTTATATACTCTTTTCAAt < 2:1525163/91‑1 (MQ=25) gaagGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAAt < 2:1677554/91‑1 (MQ=255) aagGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATa > 1:648220/1‑91 (MQ=255) aagGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATa > 1:2298354/1‑91 (MQ=255) ggTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGt < 1:2663702/91‑1 (MQ=255) gtgtACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAg < 2:2547144/89‑1 (MQ=255) gtgtACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAg > 1:2547144/1‑89 (MQ=255) tgtACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGa > 1:2583219/1‑91 (MQ=255) tACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAgg > 2:1772591/1‑91 (MQ=255) aCTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGt > 2:2165677/1‑91 (MQ=255) aCTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGt > 1:3667798/1‑91 (MQ=255) cTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGta > 1:2656230/1‑91 (MQ=255) atataAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATg > 1:2287357/1‑91 (MQ=255) ataAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGat > 2:2548520/1‑91 (MQ=255) taAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGata > 2:313482/1‑91 (MQ=255) aaGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGataa > 2:1762456/1‑91 (MQ=255) aaGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGataa < 2:2726656/91‑1 (MQ=255) aGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGataat > 2:1408830/1‑91 (MQ=255) aGTATATAGCGATGGTCATACCCATACACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGataat < 1:3606606/91‑1 (MQ=255) gTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGataat‑a < 1:2143309/91‑1 (MQ=255) gTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGataat‑a > 1:3318406/1‑91 (MQ=255) atataGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑aaa < 2:2947629/91‑1 (MQ=255) taGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑aaaaaa > 1:2937046/1‑91 (MQ=255) aGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑aaaaaaa > 2:2424190/1‑91 (MQ=255) gCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑aaaaaaaa > 2:428940/1‑91 (MQ=255) gCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑aaaaaaaa > 2:480543/1‑91 (MQ=255) gCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑aaaaaaaa > 1:2832194/1‑91 (MQ=255) cGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAg < 1:1202849/91‑1 (MQ=255) gATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGc < 1:1016109/91‑1 (MQ=255) gATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGc > 1:2417075/1‑91 (MQ=255) tGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGCTAAT‑AAAAAAAAGCCt > 2:3711530/1‑91 (MQ=255) gTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCtgt < 2:301600/91‑1 (MQ=255) tCATACCCATTCCCAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCtgtt > 1:2994273/1‑91 (MQ=255) tCATACCCATTCACAGTAACAATCCTCACCATTGAACAGACTATATACCCTTTTCAATAGTGAGGTACATGATAAT‑AAAAAAAAGCCtgtt > 2:3608081/1‑91 (MQ=255) tCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑aaaaaaa > 1:1926428/1‑83 (MQ=255) tCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑aaaaaaa < 2:1926428/83‑1 (MQ=255) cATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCtgttg > 2:3205153/1‑91 (MQ=255) aTACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTAAGGTATATGATAAT‑AAAAAAAAGCCtgttgt < 2:2499087/91‑1 (MQ=255) aCCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTca > 1:1617674/1‑91 (MQ=255) aCCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTca < 2:3398171/91‑1 (MQ=255) ccATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTcaca < 2:3871123/91‑1 (MQ=255) ttCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATg > 2:1789791/1‑91 (MQ=255) tCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATgg > 1:909059/1‑91 (MQ=255) cacaGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGt > 1:2354023/1‑91 (MQ=255) cacaGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAATGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGt > 2:329562/1‑91 (MQ=255) acaGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTc < 1:1575459/91‑1 (MQ=255) caGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCa > 2:2262420/1‑91 (MQ=255) caGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCa < 1:1772591/91‑1 (MQ=255) caGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCa < 1:3452079/91‑1 (MQ=255) caGTAACAAGCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCa < 2:3522648/91‑1 (MQ=255) aGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCa < 1:3021137/90‑1 (MQ=255) aGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCAt > 2:604055/1‑91 (MQ=255) aGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCAt > 1:744395/1‑91 (MQ=255) gTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATa > 1:2227700/1‑91 (MQ=255) gTAACAATCCTCACCATTGAAAAGAGTATATAACCCTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCCTa > 2:357335/1‑91 (MQ=255) aaCAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGa > 1:1894371/1‑91 (MQ=255) cAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGaca < 2:3404819/91‑1 (MQ=255) cAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑‑AAAAAAAGCCTGTTGTCACAATGGTCATAGacac < 1:3850542/91‑1 (MQ=255) aaTCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGacac < 2:1124685/91‑1 (MQ=255) aTCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACg > 1:3416523/1‑91 (MQ=255) cTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTAAAAAAAAAA‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACa < 2:2720530/91‑1 (MQ=255) tCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACAt > 2:572064/1‑91 (MQ=255) cactaTTGAAAAGGTTATATACTCTTTTCAATGGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATa > 1:2949565/5‑91 (MQ=21) cACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATa > 2:1488937/1‑91 (MQ=255) aCCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATAc < 1:1048360/91‑1 (MQ=255) ctaTTGAAAAGGTTATATACTCTTTTCAATGGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACt > 1:1021314/3‑91 (MQ=21) ctaTTGAAAAGGTTATATACTCTTTTCAATGGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACt > 1:871295/3‑91 (MQ=21) ccATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACt < 1:445324/91‑1 (MQ=255) ttGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTa > 1:1227909/1‑91 (MQ=255) ttGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTa < 1:311665/91‑1 (MQ=255) tGAAAAGGTTATATACTCTTTTCAATGGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTaa > 2:3273750/1‑91 (MQ=37) gAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTaaa > 2:705806/1‑91 (MQ=255) gAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTaaa < 2:2630029/91‑1 (MQ=255) gAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTaaa > 2:1849594/1‑91 (MQ=255) aaaaGAGTATATGACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAg > 1:3004483/1‑91 (MQ=255) aaaaGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCCGTTGTCACAATGGTCATAGACACGACATACTTTAAAg > 2:1052403/1‑91 (MQ=255) aaaGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAg < 2:185454/91‑1 (MQ=255) aaGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGt > 2:2360933/1‑91 (MQ=255) agagTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGtt < 1:2469245/91‑1 (MQ=255) agTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTc < 1:1181460/91‑1 (MQ=255) atataACCTTTTCAATAGTGAGGTATATGATCAT‑AAAAAAAAGCCTGTTGTCACAATCGTCATAGACACGACATACTTTAAAGGTTTCTGa > 1:3450158/1‑91 (MQ=255) tataACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGaa > 1:555166/1‑91 (MQ=255) ataACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGaat < 2:2298354/91‑1 (MQ=255) aaCCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGaatat < 1:2802328/91‑1 (MQ=255) aaCCTTTTCAATAGTGAGGTATATAATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGaatat < 2:2033104/91‑1 (MQ=255) | GTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAAT‑AAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATAT > USA300TCH1516_ALE/253902‑254080 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |