| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 253,992 | C→T | 6.6% | intergenic (+205/+157) | asbF → / ← USA300HOU_RS01125 | 3‑dehydroshikimate dehydratase/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 253,992 | 0 | C | T | 6.6% | 728.6 / 23.2 | 225 | intergenic (+205/+157) | asbF/USA300HOU_RS01125 | 3‑dehydroshikimate dehydratase/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (126/82); new base T (8/7); total (135/90) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.95e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 235 total aligned reads displayed.
GTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAA > USA300TCH1516_ALE/253902‑254082 | gtttgtAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAAc < 2:2711482/91‑1 (MQ=255)gtttgtAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAAc < 2:1399556/91‑1 (MQ=255) tttgtAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATAACCATTCACAGTAACAATCCTCACCATTTAAAAGAGTATATAAcc < 1:3837431/91‑1 (MQ=255) ttgtAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCt < 1:296192/91‑1 (MQ=255) tgtAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCtt < 2:2893625/91‑1 (MQ=255) gtAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCttt > 1:169661/1‑91 (MQ=255) gtAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCttt > 1:1438027/1‑91 (MQ=255) gtAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCttt > 1:397981/1‑91 (MQ=255) tAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCtttt < 1:2253928/91‑1 (MQ=255) tAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCtttt < 1:2833612/91‑1 (MQ=255) aaaGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACTATTGAAAAGGTTATATACTCTTTTc < 1:1020057/91‑1 (MQ=37) aaaGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTc > 1:13012/1‑91 (MQ=255) aaaGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTc > 1:2630029/1‑91 (MQ=255) agaagGTGTACTTTATATAAGTATATAGCGATGGTCGTACCCATTCACACTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCaa < 1:3355507/91‑1 (MQ=255) aagGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATa > 1:2298354/1‑91 (MQ=255) agGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAg < 1:1329929/91‑1 (MQ=255) tgtACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGa > 1:2583219/1‑91 (MQ=255) gtACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACTATTGAAAAGGTTATATACTCTTTTCAATGGTGAg < 2:1798118/91‑1 (MQ=21) gtACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAg < 2:2363139/91‑1 (MQ=255) aCTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGt > 1:3667798/1‑91 (MQ=255) cTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGta > 1:2666582/1‑91 (MQ=255) tttATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGtat < 2:2179569/91‑1 (MQ=255) atataAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATg > 1:2287357/1‑91 (MQ=255) ataAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGat > 2:2548507/1‑91 (MQ=255) aaGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGataa < 1:1738770/91‑1 (MQ=255) aaGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGataa < 2:2726656/91‑1 (MQ=255) aGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGataat > 2:1494871/1‑91 (MQ=255) gTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGataata < 1:2143309/91‑1 (MQ=255) gTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGataata > 1:3318406/1‑91 (MQ=255) tatataGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGAtaataa > 2:1607298/1‑91 (MQ=255) atataGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATaaa < 2:2947628/91‑1 (MQ=255) aGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATaaaaaaa > 1:2983349/1‑91 (MQ=255) gCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATaaaaaaaa > 2:480543/1‑91 (MQ=255) gCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATaaaaaaaa > 1:2832194/1‑91 (MQ=255) gCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATaaaaaaaa > 2:428940/1‑91 (MQ=255) cGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAg < 1:1202553/91‑1 (MQ=255) gATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGc < 1:1016109/91‑1 (MQ=255) gATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGc > 1:2417075/1‑91 (MQ=255) tGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGCTAATAAAAAAAAGCCt > 2:3711530/1‑91 (MQ=255) gTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCtgt < 2:301600/91‑1 (MQ=255) gTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATCATAATAAAAAAAAGCCtgt < 2:3387836/91‑1 (MQ=255) tCATACCCATTCACAGTAACAATCCTCACCATTGAACAGACTATATACCCTTTTCAATAGTGAGGTACATGATAATAAAAAAAAGCCtgtt > 2:3608081/1‑91 (MQ=255) tCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATaaaaaaa > 1:1926428/1‑83 (MQ=255) tCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAACCAAAGCCtgtt > 2:2462055/1‑91 (MQ=255) cATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCtgttg < 2:2566829/91‑1 (MQ=255) cATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCtgttg > 1:2561875/1‑91 (MQ=255) aTACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCtgttgt > 2:1853300/1‑91 (MQ=255) cccATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTcac > 1:2386753/1‑91 (MQ=255) ttCACAGTAACAATCCTCACCATTGAAAAGCGTATATAAACTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATg > 1:1617654/1‑91 (MQ=255) ttCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATg > 1:1632726/1‑91 (MQ=255) cacaGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAATGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGt > 2:329562/1‑91 (MQ=255) acaGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTc > 2:562287/1‑91 (MQ=255) acaGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTc > 2:1763986/1‑91 (MQ=255) caGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCa < 1:3452079/91‑1 (MQ=255) caGTAACAAGCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCa < 2:3522648/91‑1 (MQ=255) aGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCa < 1:3021137/90‑1 (MQ=255) aGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCAt > 1:1140625/1‑91 (MQ=255) aGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCAt > 1:744395/1‑91 (MQ=255) aGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCAt > 2:604055/1‑91 (MQ=255) aGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCAt > 2:1714549/1‑91 (MQ=255) gTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATa < 2:1975122/91‑1 (MQ=255) aaCAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGa > 1:1894371/1‑91 (MQ=255) cAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGaca < 2:3404819/91‑1 (MQ=255) aaTCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGacac < 2:1124685/91‑1 (MQ=255) aTCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACg > 1:3416523/1‑91 (MQ=255) aTCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACg > 2:2537836/1‑91 (MQ=255) tCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGa > 2:2061822/1‑91 (MQ=255) ccTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGAc < 1:2658840/91‑1 (MQ=255) cTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACa > 1:2906168/1‑91 (MQ=255) cTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACa < 1:1375174/91‑1 (MQ=255) tCACTATTGAAAAGGTTATATACTCTTTTCAATGGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACAt > 2:2115872/1‑91 (MQ=21) cactaTTGAAAAGGTTATATACTCTTTTCAATGGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATa > 1:2949565/5‑91 (MQ=21) actaTTGAAAAGGTTATATACTCTTTTCAATGGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATAc > 2:1433467/4‑91 (MQ=21) aCCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATAc < 1:706409/91‑1 (MQ=255) ctaTTGAAAAGGTTATATACTCTTTTCAATGGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACt > 1:1021314/3‑91 (MQ=21) ctaTTGAAAAGGTTATATACTCTTTTCAATGGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACt > 2:3545310/3‑91 (MQ=21) ctaTTGAAAAGGTTATATACTCTTTTCAATGGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACt > 1:871295/3‑91 (MQ=21) ccATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACt < 1:445324/91‑1 (MQ=255) ttGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTa > 1:1227909/1‑91 (MQ=255) ttGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTa < 1:311665/91‑1 (MQ=255) tGAAAAGGTTATATACTCTTTTCAATGGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTaa > 2:3273750/1‑91 (MQ=37) gAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTaaa > 2:705806/1‑91 (MQ=255) aaaaGAGTATATGACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAg > 1:3004483/1‑91 (MQ=255) aaaaGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAg > 1:935574/1‑91 (MQ=255) aaaaGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCCGTTGTCACAATGGTCATAGACACGACATACTTTAAAg > 2:1052403/1‑91 (MQ=255) aaGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGt > 2:2360566/1‑91 (MQ=255) agagTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGtt > 2:188240/1‑91 (MQ=255) agagTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGtt < 1:2469245/91‑1 (MQ=255) gagTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGttt < 2:3162124/91‑1 (MQ=255) agTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTcac > 1:363440/1‑57 (MQ=255) agTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTc < 1:1180680/91‑1 (MQ=255) gTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGttt < 2:228683/89‑1 (MQ=255) gTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCt > 1:1271712/1‑91 (MQ=255) gTATATAACCTTTTCAATAGTGAGGTATATGAAAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCt < 2:3054561/91‑1 (MQ=255) atataACCTTTTCAATAGTGAGGTATATGATCATAAAAAAAAGCCTGTTGTCACAATCGTCATAGACACGACATACTTTAAAGGTTTCTGa > 1:3450158/1‑91 (MQ=255) tataACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGaa > 1:555166/1‑91 (MQ=255) aaCCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGaatat < 2:1774186/91‑1 (MQ=255) aaCCTTTTCAATAGTGAGGTATATGAAAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGaatat < 2:3785703/91‑1 (MQ=255) ccTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGaatataa > 1:2719075/1‑91 (MQ=255) ccTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGaatataa > 1:1840307/1‑91 (MQ=255) | GTTTGTAAAGAAGGTGTACTTTATATAAGTATATAGCGATGGTCATACCCATTCACAGTAACAATCCTCACCATTGAAAAGAGTATATAACCTTTTCAATAGTGAGGTATATGATAATAAAAAAAAGCCTGTTGTCACAATGGTCATAGACACGACATACTTTAAAGGTTTCTGAATATAA > USA300TCH1516_ALE/253902‑254082 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |