Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,931,5990AT20.1% 599.1 / 148.8 249L1217I (TTA→ATA) USA300HOU_RS09605hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (106/93);  new base T (20/30);  total (126/123)
Fisher's exact test for biased strand distribution p-value = 1.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.72e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 281 total aligned reads displayed.

TGTACCACCATTCTTGTCTATCTCGAAGAGAAATTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAATACATATTGTTTCACCAAATTTAGTTTTCCA  >  USA300TCH1516_ALE/1931509‑1931689
                                                                                          |                                                                                          
tGTACCACCATTCTTGTCTATCTCGAAGAGAAATTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTtaa                                                                                            >  1:2560710/1‑91 (MQ=255)
tGTACCACCATTCTTGTCAATCTCGAAGAGAAATTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTtaa                                                                                            <  2:387755/91‑1 (MQ=255)
 gTACCACCATTCTTGTCTATCTCGAAGAGAAATTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTtaat                                                                                           <  2:906366/91‑1 (MQ=255)
  tACCACCATTCTTGTCTATCTCGAAGAGAAATTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTtaata                                                                                          >  2:151457/1‑91 (MQ=255)
  tACCACCATTCTTGTCTATCTCGAAGAGAAATTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTtaata                                                                                          >  2:491769/1‑91 (MQ=255)
   accaccATTCTTGTCTATCTCGAAGAGAAATTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTtattaa                                                                                         <  1:2934319/91‑1 (MQ=255)
   accaccATTCTTGTCTATCTCGAAGAGAAATTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTtaataa                                                                                         >  1:2686172/1‑91 (MQ=255)
   accaccATTCTTGTCTATCTCGAAGAGAAATTTTTTCTAGATATATTTTCCATCCAATAGACAAGTTTTTAGTATTAAGAAAGTTtattaa                                                                                         <  2:1771393/91‑1 (MQ=255)
    ccaccaTTCTTGTCTATCTCGAAGAGAAATTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTATTaaa                                                                                        <  2:153915/91‑1 (MQ=255)
    ccaccaTTCTTGTCTATCTCGAAGAGAAATTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTATTaaa                                                                                        <  1:401712/91‑1 (MQ=255)
    ccaccaTTCTTGTCTATCTCGAAGAGAAATTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTATTaaa                                                                                        <  1:1328708/91‑1 (MQ=255)
    ccaccaTTCTTGTCTATCTCGAAGAGAAATTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTATTaaa                                                                                        <  1:1230394/91‑1 (MQ=255)
    ccaccaTTCTTGTCTATCTCGAAGAGAAATTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATaaa                                                                                        <  1:530024/91‑1 (MQ=255)
     caccaTTCTTGTCTATCTCGAAGAGAAATTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTATTAAAc                                                                                       <  2:2091144/91‑1 (MQ=255)
     caccaTTCTTGTCTATCTCGAAGAGAAATTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAAc                                                                                       <  2:32393/91‑1 (MQ=255)
      accaTTCTTGTCTATCTCGAAGAGAAATTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTATTAAAct                                                                                      <  1:3004989/91‑1 (MQ=255)
      accaTTCTTGTCTATCTCGAAGAGAAATTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTATTAAAct                                                                                      <  1:2262987/91‑1 (MQ=255)
      accaTTCTTGTCTATCTCGAAGAGAAATTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAAct                                                                                      <  2:1409178/91‑1 (MQ=255)
       ccaTTCTTGTCTCTCTCGAAGAGAAATTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAGctt                                                                                     >  1:2394386/1‑91 (MQ=255)
       ccaTTCTTGTCTATCTCGAAGAGAAATTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTATTAAActt                                                                                     <  2:1482299/91‑1 (MQ=255)
       ccaTTCTTGTCTATCTCGAAGAGAAATTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTATTAAActt                                                                                     <  1:1271244/91‑1 (MQ=255)
       ccaTTCTTGTCTATCTCGAAGAGAAATTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAActt                                                                                     >  2:760507/1‑91 (MQ=255)
        caTTCTTGTCTATCTCGAAGAGAAATTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTATTAAActtt                                                                                    <  2:581620/91‑1 (MQ=255)
         aTTCTTGTCTATCTCGAAGAGAAATCTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAActttc                                                                                   <  1:2887145/91‑1 (MQ=255)
           tCTTGTCTATCTCGAAGAGAAATTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAActttctt                                                                                 <  2:449101/91‑1 (MQ=255)
            cTTGTCTATCTCGAAGAGAAATTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTATTAAActttctta                                                                                <  1:151656/91‑2 (MQ=255)
              tGTCTATCTCGAAGAGAAATTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTATTAAActttcttaat                                                                              <  1:1583406/91‑4 (MQ=255)
               gTCTATCTCGAAGAGAAATTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCtttttt                                                                             <  1:1345316/91‑1 (MQ=255)
                 cTATCTCGAAGAGAAATTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTtaa                                                                           >  1:2024033/1‑91 (MQ=255)
                  tATCTCGAAGAGAAATTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTtaat                                                                          >  2:2584404/1‑91 (MQ=255)
                        gAAGAGAAATTTTTTTTATATTTATTTTCCATCAAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTaa                                                                    >  2:2477020/1‑91 (MQ=255)
                        gAAGAGAAATTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGCAAGTTTAATAAACTTTCTTTTTTAATAAGTaa                                                                    >  2:2848729/1‑91 (MQ=255)
                          agagAAATTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAAc                                                                  <  2:1129256/91‑1 (MQ=255)
                           gagaAATTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAAca                                                                 >  1:2421264/1‑91 (MQ=255)
                             gaAATTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAAcatt                                                               >  2:1198372/1‑91 (MQ=255)
                              aaaTTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAAcattc                                                              <  2:1966826/91‑1 (MQ=255)
                              aaaTTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAAcattc                                                              >  2:1885505/1‑91 (MQ=255)
                               aaTTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAAcattca                                                             <  1:1990077/91‑1 (MQ=255)
                               aaTTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAAcattca                                                             >  2:1656869/1‑91 (MQ=255)
                                aTTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAAcattcat                                                            >  1:305616/1‑91 (MQ=255)
                                 ttttttCTNGATNTNTTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAAcattcatt                                                           <  1:83594/91‑1 (MQ=255)
                                   ttttCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTAt                                                         <  2:2674762/91‑1 (MQ=255)
                                   ttttCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTAt                                                         <  1:1217480/91‑1 (MQ=255)
                                   ttttCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTAt                                                         <  2:1813017/91‑1 (MQ=255)
                                    tttCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATc                                                        <  1:1323688/91‑1 (MQ=255)
                                    tttCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATc                                                        <  1:3067401/91‑1 (MQ=255)
                                       cTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCaaa                                                     >  2:1305031/1‑91 (MQ=255)
                                         aGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAAtt                                                   >  2:2084575/1‑91 (MQ=255)
                                          gATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTc                                                  >  1:655276/1‑91 (MQ=255)
                                             atatTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAg                                               <  2:1578506/91‑1 (MQ=255)
                                                ttttCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTaa                                            >  1:2182512/1‑91 (MQ=255)
                                                ttttCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTaa                                            >  2:46957/1‑91 (MQ=255)
                                                 tttCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAAt                                           >  1:272301/1‑91 (MQ=255)
                                                   tccatccaATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAAttt                                         <  2:718574/91‑1 (MQ=255)
                                                    ccatccaATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTa                                        >  2:92680/1‑91 (MQ=255)
                                                     catccaATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAg                                       <  1:2442911/91‑1 (MQ=255)
                                                     catccaATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAg                                       <  1:2319285/91‑1 (MQ=255)
                                                      atccaATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGa                                      >  1:914638/1‑91 (MQ=255)
                                                      atccaATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGa                                      >  1:1424989/1‑91 (MQ=255)
                                                         caATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAAtt                                   >  1:610437/1‑91 (MQ=255)
                                                           aTAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTaa                                 >  1:2938820/1‑91 (MQ=255)
                                                            tAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAAt                                >  1:908615/1‑91 (MQ=255)
                                                              gACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAATAc                              >  2:421339/1‑91 (MQ=255)
                                                               aCAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAATACa                             <  1:321327/91‑1 (MQ=255)
                                                                cAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAATACat                            <  2:10023/91‑1 (MQ=255)
                                                                cAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAATACat                            <  2:3100281/91‑1 (MQ=255)
                                                                 aaTTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAATACata                           <  2:347423/91‑1 (MQ=255)
                                                                  aTTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAATACatat                          <  2:1839291/91‑1 (MQ=255)
                                                                   ttttttAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAATACATAtt                         >  2:2990735/1‑91 (MQ=255)
                                                                   ttttttAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAATACATAtt                         >  2:2135950/1‑91 (MQ=255)
                                                                   ttttttAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAATACATAtt                         >  2:2235704/1‑91 (MQ=255)
                                                                     ttttAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAATACATATTGt                       >  2:2788958/1‑91 (MQ=255)
                                                                      tttAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAATACATATTGtt                      >  1:464100/1‑91 (MQ=255)
                                                                      tttAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAATACATATTGtt                      <  2:2431260/91‑1 (MQ=255)
                                                                        tAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAATACCTATTGTTTc                    >  1:2964942/1‑91 (MQ=255)
                                                                         tgTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAATACATATTGTTTCa                   <  1:1228606/90‑1 (MQ=255)
                                                                         aGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAATACATATTGTTTCa                   <  1:1365871/91‑1 (MQ=255)
                                                                         aGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAATACATATTGTTTCa                   >  1:49433/1‑91 (MQ=255)
                                                                            aTTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAATACATATTGTTTCACCa                >  2:871289/1‑91 (MQ=255)
                                                                              tAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAGACATTCATTATCAAATTCGAGTAATTTAGAATTAATACATATTGTTTCACCaaa              <  2:2797466/91‑1 (MQ=255)
                                                                              tAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAATACATATTGTTTCACCaaa              <  2:1322676/91‑1 (MQ=255)
                                                                               aaGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAATACATATTGTTTCACCAAAt             >  2:1842421/1‑91 (MQ=255)
                                                                                aGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAATACATATTGTTTCACCAAAtt            <  1:553469/91‑1 (MQ=255)
                                                                                aGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAATACATATTGTTTCACCAAAtt            <  1:1052689/91‑1 (MQ=255)
                                                                                    aGTTTATTAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAATACATATTGTTTCACCAAATTTAGt        >  1:1021878/1‑91 (MQ=255)
                                                                                    aGTTTATTAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAATACATATTGTTTCACCAAATTTAGt        >  2:2720011/1‑91 (MQ=255)
                                                                                    aGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAATACATATTGTTTCACCAAATTTAGt        >  1:1842138/1‑91 (MQ=255)
                                                                                     gTTTATTAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTACTTTATAATTAATACATACTCTTTCCCCAAATTTAGtt       >  2:2372599/1‑91 (MQ=255)
                                                                                     gTTTATTAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAATACATATTGTTTCACCAAATTTAGtt       >  1:2524841/1‑91 (MQ=255)
                                                                                     gTTTATTAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAATACATATTGTTTCACCAAATTTAGtt       >  1:2605353/1‑91 (MQ=255)
                                                                                     gTTTATTAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAATACATATTGTTTCACCAAATTTAGtt       >  2:1534432/1‑91 (MQ=255)
                                                                                     gTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAATACATATTGTTTCACCAAATTTAGtt       >  2:685533/1‑91 (MQ=255)
                                                                                      tttAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAATACATATTGTTTCACCAAATTTAGttt      >  2:2175797/1‑91 (MQ=255)
                                                                                      tttAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAATACATATTGTTTCACCAAATTTAGttt      <  1:838864/91‑1 (MQ=255)
                                                                                      tttAATAAACTTTCTTTTTTAATAAGTAAACATTCATTACCAAATTCGAGTAATTTAGAATTAATACATATTGTTTCACCAAATTTAGttt      <  2:1852913/91‑1 (MQ=255)
                                                                                        taataaACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAATACATATTGTTTCACCAAATTTAGTTTTc    <  1:2765392/91‑1 (MQ=255)
                                                                                        taataaACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAATACATATTGTTTCACCAAATTTAGTTTTc    >  2:652320/1‑91 (MQ=255)
                                                                                         aataaACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAATACATATTGTTTCACCAAATTTAGTTTTcc   >  2:2319716/1‑91 (MQ=255)
                                                                                          ataaACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAATACATATTGTTTCACCAAATTTAGTTTTCCa  <  1:1642878/91‑1 (MQ=255)
                                                                                          ataaACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAATACATATTGTTTCACCAAATTTAGTTTTCCa  >  1:137799/1‑91 (MQ=255)
                                                                                          |                                                                                          
TGTACCACCATTCTTGTCTATCTCGAAGAGAAATTTTTTCTAGATATATTTTCCATCCAATAGACAATTTTTTAGTATTAAGAAAGTTTAATAAACTTTCTTTTTTAATAAGTAAACATTCATTATCAAATTCGAGTAATTTAGAATTAATACATATTGTTTCACCAAATTTAGTTTTCCA  >  USA300TCH1516_ALE/1931509‑1931689

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑