Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,343,4380CT40.2% 124.5 / 206.3 174intergenic (+114/+250)USA300HOU_RS11955/aldC_165 kDa membrane protein/Alpha‑acetolactate decarboxylase
Reads supporting (aligned to +/- strand):  ref base C (54/50);  new base T (33/37);  total (87/87)
Fisher's exact test for biased strand distribution p-value = 6.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.44e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 180 total aligned reads displayed.

GCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTCA  >  USA300TCH1516_ALE/2343348‑2343528
                                                                                          |                                                                                          
gCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATAc                                                                                            >  2:159821/1‑91 (MQ=255)
gCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATAc                                                                                            >  1:126063/1‑91 (MQ=255)
 cAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACa                                                                                           >  1:2055828/1‑91 (MQ=255)
  aaGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACaa                                                                                          >  1:1413486/1‑91 (MQ=255)
   aGCGCTGAAAAATTGGCGTTTGTGCTTTTAGTTATTGAACTTGGAAATTATTGTGGTTTGCTTCTCGTAGTAACAGATTGGCCGATACAAt                                                                                         <  2:2881293/91‑1 (MQ=255)
   aGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAAt                                                                                         <  2:1427388/91‑1 (MQ=255)
    gcgcTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATa                                                                                        >  1:2189021/1‑91 (MQ=255)
      gcTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGt                                                                                      >  2:2591390/1‑91 (MQ=255)
       cTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTa                                                                                     >  2:2535825/1‑91 (MQ=255)
       cTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTa                                                                                     <  1:1062634/91‑1 (MQ=255)
        tGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTTGTAGTAACAGATTGGCCTATACAATAGTaa                                                                                    >  1:1097030/1‑91 (MQ=255)
        tGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTaa                                                                                    >  2:1807834/1‑91 (MQ=255)
        tGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTaa                                                                                    <  2:299410/91‑1 (MQ=255)
        tGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTaa                                                                                    <  2:1072700/91‑1 (MQ=255)
        tGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTaa                                                                                    >  1:1505696/1‑91 (MQ=255)
        tGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTaa                                                                                    <  2:298614/91‑1 (MQ=255)
        tGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTaa                                                                                    >  1:1211089/1‑91 (MQ=255)
          aaaaaTTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAAt                                                                                  >  2:1611799/1‑91 (MQ=255)
          aaaaaTTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAAt                                                                                  >  2:150610/1‑91 (MQ=255)
          aaaaaTTGGCGTTTGTCTTTTTAGTCATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAAt                                                                                  <  1:2872706/91‑1 (MQ=255)
            aaaTTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAAttt                                                                                <  1:1374829/91‑1 (MQ=255)
            aaaTTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAAttt                                                                                <  1:365033/91‑1 (MQ=255)
            aaaTTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAAttt                                                                                <  2:2945423/91‑1 (MQ=255)
            aaaTTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAAttt                                                                                <  1:1897966/91‑1 (MQ=255)
               ttGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTtat                                                                             <  1:1712320/91‑1 (MQ=255)
               ttGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTtat                                                                             >  1:818973/1‑91 (MQ=255)
               ttGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTtat                                                                             >  1:818801/1‑91 (MQ=255)
               ttGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTtat                                                                             >  1:1046112/1‑91 (MQ=255)
                tGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTtata                                                                            <  1:2494284/91‑1 (MQ=255)
                tGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTtata                                                                            >  1:2241001/1‑91 (MQ=255)
                tGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTtata                                                                            <  1:1861101/91‑1 (MQ=255)
                 ggCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTatat                                                                           >  1:3095970/1‑91 (MQ=255)
                  gCGTTTGTCTTTTTAGTTATTGAACTTGTAACTTATTGTTGCTTGCTTCTCGTAGTAACAGATTGGCGCATATAATAGTCAATTTTATATg                                                                          >  2:2357568/1‑91 (MQ=255)
                  gCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATg                                                                          <  2:2335932/91‑1 (MQ=255)
                   cGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACTGATTGGCCTATACAATAGTAAATTTTATATGa                                                                         >  2:2953820/1‑91 (MQ=255)
                   cGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGa                                                                         <  1:716274/91‑1 (MQ=255)
                          cTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTa                                                                  <  1:904256/91‑1 (MQ=255)
                             tttAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACca                                                               >  2:2086945/1‑91 (MQ=255)
                             tttAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACca                                                               <  1:315881/91‑1 (MQ=255)
                              ttAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACcac                                                              <  1:1338818/91‑1 (MQ=255)
                               tAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACcaca                                                             <  1:252435/91‑1 (MQ=255)
                               tAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACcaca                                                             <  1:1952620/91‑1 (MQ=255)
                                 gTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAAt                                                           >  2:709834/1‑91 (MQ=255)
                                 gTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAAt                                                           >  2:613782/1‑91 (MQ=255)
                                   tATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAAttt                                                         >  1:673870/1‑91 (MQ=255)
                                   tATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAAttt                                                         <  1:2940078/91‑1 (MQ=255)
                                    aTTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTta                                                        >  1:442408/1‑91 (MQ=255)
                                    aTTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTta                                                        >  2:40625/1‑91 (MQ=255)
                                    aTTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTta                                                        >  1:2504889/1‑91 (MQ=255)
                                         actcgTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATc                                                   <  2:436343/87‑1 (MQ=255)
                                         aCTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATc                                                   <  1:2174253/91‑1 (MQ=255)
                                         aCTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATc                                                   <  2:2206534/91‑1 (MQ=255)
                                             gTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTaaa                                               >  2:3022991/1‑91 (MQ=255)
                                              tAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAAc                                              >  2:3098951/1‑91 (MQ=255)
                                                 attattGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATa                                           <  2:565827/91‑1 (MQ=255)
                                                 attattGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATa                                           >  1:1039610/1‑91 (MQ=255)
                                                 attattGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATa                                           >  2:1531531/1‑91 (MQ=255)
                                                   tattGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAAc                                         <  1:194092/91‑1 (MQ=255)
                                                    attGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACa                                        >  2:63096/1‑91 (MQ=255)
                                                    attGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACa                                        <  1:1859210/91‑1 (MQ=255)
                                                    attGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACa                                        <  2:1763529/91‑1 (MQ=255)
                                                    attGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACa                                        <  1:542561/91‑1 (MQ=255)
                                                    attGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACa                                        <  1:542402/91‑1 (MQ=255)
                                                      tgttgtTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGc                                      >  2:985140/1‑91 (MQ=255)
                                                      tgttgtTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGc                                      >  1:1862487/1‑91 (MQ=255)
                                                      tgttgtTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGc                                      >  2:1026922/1‑91 (MQ=255)
                                                         tgttTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCaaa                                   >  2:2401228/1‑91 (MQ=255)
                                                         tgttTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCaaa                                   >  2:2401063/1‑91 (MQ=255)
                                                           tttGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAAtc                                 <  2:2089238/91‑1 (MQ=255)
                                                             tGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAAtctc                               <  1:220774/91‑1 (MQ=255)
                                                              gCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCa                              <  1:1966705/91‑1 (MQ=255)
                                                               cTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCaa                             >  2:2780844/1‑91 (MQ=255)
                                                                ttCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCaaa                            <  1:2810153/91‑1 (MQ=255)
                                                                 tctcGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAat                           <  1:758426/91‑1 (MQ=255)
                                                                    cGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATc                        >  2:1113802/1‑91 (MQ=255)
                                                                    cGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATc                        >  2:1113604/1‑91 (MQ=255)
                                                                    cGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATc                        >  1:1959494/1‑91 (MQ=255)
                                                                    cGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATc                        >  1:2955037/1‑91 (MQ=255)
                                                                    cGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGAGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATc                        >  2:2386123/1‑91 (MQ=255)
                                                                    cGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATc                        >  2:2985065/1‑91 (MQ=255)
                                                                     gtagtaACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCt                       <  1:2937225/91‑1 (MQ=255)
                                                                      tagtaACAGATTGGCCTATACAATAGTAAATTTTATATGATTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTg                      <  1:142794/91‑1 (MQ=255)
                                                                      tagtaACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTg                      >  1:394392/1‑91 (MQ=255)
                                                                          aaCAGATTGGCCTATACCATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAAc                  <  1:2142232/91‑1 (MQ=255)
                                                                           aCAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCGCAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAAcc                 <  2:2774347/91‑1 (MQ=255)
                                                                           aCAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAAcc                 >  2:826305/1‑91 (MQ=255)
                                                                             aGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCta               >  2:2462460/1‑91 (MQ=255)
                                                                               aTTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCtaat             <  1:2701166/91‑1 (MQ=255)
                                                                                ttGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCtaata            <  2:1262156/91‑1 (MQ=255)
                                                                                  ggCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATaaa          <  1:1483872/91‑1 (MQ=255)
                                                                                  ggCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATaaa          <  1:1484273/91‑1 (MQ=255)
                                                                                  ggCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATaaa          <  2:1944242/91‑1 (MQ=255)
                                                                                   gCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAAt         >  2:174887/1‑91 (MQ=255)
                                                                                    ccTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATc        <  2:18646/91‑1 (MQ=255)
                                                                                    ccTATACAATAGTAAATTTTATATGGGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATc        <  2:668764/91‑1 (MQ=255)
                                                                                    ccTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATc        >  2:661352/1‑91 (MQ=255)
                                                                                     cTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATcc       <  1:2527585/91‑1 (MQ=255)
                                                                                       ataCAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATcccc     >  2:1450698/1‑91 (MQ=255)
                                                                                        taCAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCt    >  1:2198825/1‑91 (MQ=255)
                                                                                          cAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATACCTGGAACCTAATAAATCCCCTCa  <  1:1232957/91‑1 (MQ=255)
                                                                                          |                                                                                          
GCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTCA  >  USA300TCH1516_ALE/2343348‑2343528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑