Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,372,4130CG23.5% 509.5 / 153.1 223intergenic (+164/+28)USA300TCH1516_02262/pbuGhypothetical protein/Guanine/hypoxanthine permease PbuG
Reads supporting (aligned to +/- strand):  ref base C (79/90);  new base G (19/33);  total (100/123)
Fisher's exact test for biased strand distribution p-value = 2.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 229 total aligned reads displayed.

TATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATG  >  USA300TCH1516_ALE/2372323‑2372503
                                                                                          |                                                                                           
     gATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTGCCAATGATTgg                                                                                        <  2:1023827/91‑1 (MQ=255)
     gATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTGCCAATGATTgg                                                                                        <  1:1268451/91‑1 (MQ=255)
       tatTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTGCCAATGATTggta                                                                                      <  1:180762/91‑3 (MQ=39)
       tatTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCa                                                                                      <  2:1489568/91‑1 (MQ=255)
        atTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAg                                                                                     <  1:2759199/91‑1 (MQ=255)
        atTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAg                                                                                     >  2:1704752/1‑91 (MQ=255)
        atTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAg                                                                                     <  1:2946787/91‑1 (MQ=255)
        atTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAg                                                                                     <  2:2646914/91‑1 (MQ=255)
        atTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAg                                                                                     <  2:1544545/91‑1 (MQ=255)
         tttAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTGCCAATGATTGGTAGt                                                                                    <  1:319377/91‑1 (MQ=38)
         tttAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGt                                                                                    >  1:97814/1‑91 (MQ=255)
         tttAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGt                                                                                    <  2:2225663/91‑1 (MQ=255)
         tttAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGt                                                                                    >  1:1923545/1‑91 (MQ=255)
          ttAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTc                                                                                   <  2:2445945/91‑1 (MQ=255)
          ttAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTA‑TTTATCCTTACTTCAAAGAAAAAAACGACTGCCAATGATTGGTAGTCg                                                                                  <  2:533994/91‑1 (MQ=37)
           tAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTGCCAATGATTGGTAGTCg                                                                                  <  1:2362910/91‑1 (MQ=38)
           tAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACCTCAAAGAAAAAAACGACTGCCAATGATTGGTAGTCg                                                                                  <  1:675167/91‑1 (MQ=38)
            acaGACATTCATATTATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTGCCAATGATTGGTAGTCG‑t                                                                                <  2:1145608/89‑1 (MQ=37)
            aatgACATTCGTAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAATGAAAAAAACGACTGCCGATGATTGGTAGTCG‑t                                                                                <  2:1301339/88‑1 (MQ=37)
            aaaGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTGCCAATGATTGGTAGTCG‑t                                                                                <  2:1301941/91‑1 (MQ=38)
            aaaGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTGCCAATGATTGGTAGTCG‑t                                                                                <  1:2089214/91‑1 (MQ=38)
            aaaGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTGCCAATGATTGGTAGTCG‑t                                                                                <  2:1712971/91‑1 (MQ=38)
            aaaGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCG‑t                                                                                >  2:2076840/1‑91 (MQ=255)
            aaaGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCG‑t                                                                                <  2:865503/91‑1 (MQ=255)
             aaGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCG‑tt                                                                               <  2:1479303/91‑1 (MQ=255)
              aGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCG‑ttt                                                                              <  1:2606341/91‑1 (MQ=255)
                aCATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTGCCAATGATTGGTAGTCG‑ttttt                                                                            <  2:690190/91‑1 (MQ=37)
                aCATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCG‑ttttt                                                                            <  2:1898527/91‑1 (MQ=255)
                aCATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCG‑ttttt                                                                            <  2:547221/91‑1 (MQ=255)
                aCATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCG‑ttttt                                                                            <  1:2233500/91‑1 (MQ=255)
                 cATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCG‑tttttt                                                                           <  1:701128/91‑1 (MQ=255)
                  aTTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCG‑TTTTTtg                                                                          <  1:171402/91‑1 (MQ=255)
                     cATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCG‑TTTTTTgtgt                                                                       >  2:707984/1‑91 (MQ=255)
                      ataataTCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTc                                                                      <  1:2180856/91‑1 (MQ=255)
                       taataTCGCGTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCt                                                                     <  1:2975326/91‑1 (MQ=255)
                         atatCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTgg                                                                   >  1:2518071/1‑91 (MQ=255)
                          tatCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGa                                                                  <  1:1888255/91‑1 (MQ=255)
                           atCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATTATTGGCAGTCA‑TTTTTTGTGTCTGGAt                                                                 >  2:1967256/1‑91 (MQ=255)
                             cGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGAttt                                                               <  1:835093/91‑1 (MQ=255)
                             cGCTTTATGTTATTTCGCTTCTGATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGAttt                                                               <  2:2498773/91‑1 (MQ=255)
                                tttATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAAc                                                            >  1:2001226/1‑91 (MQ=255)
                                    tGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATg                                                        <  1:770942/91‑1 (MQ=255)
                                        aTTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCGGTCG‑TCATTTGTGTCTGGATTTAACCATGAACa                                                    >  2:1657479/1‑91 (MQ=255)
                                        aTTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACa                                                    >  2:263169/1‑91 (MQ=255)
                                         tttCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACaa                                                   >  2:3081115/1‑91 (MQ=255)
                                         tttCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACaa                                                   >  1:1041505/1‑91 (MQ=255)
                                          ttCGCTTGTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACTATGAACaaa                                                  <  1:1553357/91‑1 (MQ=255)
                                            cGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAata                                                >  1:2153713/1‑91 (MQ=255)
                                             gCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAatat                                               >  1:2732814/1‑91 (MQ=255)
                                               ttcttATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGa                                             <  2:194997/91‑1 (MQ=255)
                                               ttcttATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGa                                             >  2:1026858/1‑91 (MQ=255)
                                                 cttATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAg                                           <  2:977633/91‑1 (MQ=255)
                                                 cttATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAg                                           >  1:2735876/1‑91 (MQ=255)
                                                   tATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTa                                         >  2:335365/1‑91 (MQ=255)
                                                   tATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTa                                         <  2:2329405/91‑1 (MQ=255)
                                                   tATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTa                                         >  1:2950858/1‑91 (MQ=255)
                                                      tttATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAg                                      <  1:1520129/91‑1 (MQ=255)
                                                       ttATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAga                                     >  1:1288832/1‑91 (MQ=255)
                                                       ttATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAga                                     <  1:2812575/91‑1 (MQ=255)
                                                        tATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAgaa                                    >  2:226034/1‑91 (MQ=255)
                                                         aTCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAgaat                                   <  2:735543/91‑1 (MQ=255)
                                                            cTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAgaatgaa                                <  2:841936/91‑1 (MQ=255)
                                                            cTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAgaatgaa                                <  1:500073/91‑1 (MQ=255)
                                                               aCTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAg                             <  2:3034125/91‑1 (MQ=255)
                                                                cTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGt                            <  2:1283880/91‑1 (MQ=255)
                                                                cTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGt                            <  1:1125086/91‑1 (MQ=255)
                                                                 ttCAAAGAAAAAAACGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTa                           <  2:2131192/91‑1 (MQ=255)
                                                                  tCAAAGAAAAAAACGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTAc                          <  2:1568752/91‑1 (MQ=255)
                                                                   cAAAGAAAAAAACGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTAcc                         <  1:1086936/91‑1 (MQ=255)
                                                                    aaaGAAAAAAACGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCa                        <  1:3036295/91‑1 (MQ=255)
                                                                      aGAAAAAAACGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCAtt                      >  2:902639/1‑91 (MQ=255)
                                                                          aaaaaCGACTGCCAATGATTGGTAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAata                  >  1:2542540/1‑91 (MQ=37)
                                                                           aaaaCGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAatac                 <  2:2473989/91‑1 (MQ=255)
                                                                           aaaaCGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAatac                 <  2:2620059/91‑1 (MQ=255)
                                                                            aaaCGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAataca                >  1:2466835/1‑91 (MQ=255)
                                                                            aaaCGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAataca                >  1:2676987/1‑91 (MQ=255)
                                                                            aaaCGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAataca                >  1:2024510/1‑91 (MQ=255)
                                                                             aaCGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAatacat               >  1:1947448/1‑91 (MQ=255)
                                                                             aaCGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAatacat               <  1:862630/91‑1 (MQ=255)
                                                                             aaCGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAatacat               >  1:2674215/1‑91 (MQ=255)
                                                                             aaCGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAatacat               <  1:576361/91‑1 (MQ=255)
                                                                             aaCGACTACCAAGCATTGGCAGTCG‑TTGTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAatacat               <  2:1187446/91‑1 (MQ=255)
                                                                              aCGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAatacata              >  2:2995860/1‑91 (MQ=255)
                                                                               cGACTGCCAATGATTGGTAGTCGTTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAatacata              >  1:2286476/1‑91 (MQ=37)
                                                                               cGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAatacatac             <  2:1995800/91‑1 (MQ=255)
                                                                               cGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAatacatac             <  1:439684/91‑1 (MQ=255)
                                                                                gACTACCAATCATTGGGAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAAtacataca            <  2:2964405/91‑1 (MQ=255)
                                                                                gACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAAtacataca            <  1:2039383/91‑1 (MQ=255)
                                                                                gACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAAtacataca            >  2:472619/1‑91 (MQ=255)
                                                                                  ctgccAATGATTGGTAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACAtt          >  1:129729/4‑91 (MQ=39)
                                                                                  ctgccAATGATTGGTAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACAtt          >  1:2652781/4‑91 (MQ=255)
                                                                                  ctgccAATGATTGGTAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACAtt          >  1:2929128/4‑91 (MQ=39)
                                                                                  ctgccAATGATTGGTAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACAtt          >  1:1007519/4‑91 (MQ=255)
                                                                                   tACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTa         <  1:327511/91‑1 (MQ=255)
                                                                                    gccAATGATTGGTAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACAAACATTAt        >  2:2749453/2‑91 (MQ=39)
                                                                                     ccAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACTTTATc       >  2:2853186/1‑91 (MQ=255)
                                                                                     ccAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATc       >  2:2466847/1‑91 (MQ=255)
                                                                                        aTCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGa    <  1:923/91‑1 (MQ=255)
                                                                                        aTCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGa    <  2:2166733/91‑1 (MQ=255)
                                                                                          cATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATg  <  1:1583300/91‑1 (MQ=255)
                                                                                          |                                                                                           
TATCGGATATTTAAAGACATTCATAATATCGCTTTATGTTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCG‑TTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATG  >  USA300TCH1516_ALE/2372323‑2372503

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑