BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	1,002,200	T→G	M173R (ATG→AGG)	mecA →	Adapter protein MecA 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,002,200	0	T	G	97.9%	678.9 / 3.4	188	M173R (ATG→AGG)	mecA	Adapter protein MecA 1
Reads supporting (aligned to +/- strand): ref base T (1/3); new base G (81/103); total (82/106)
Fisher's exact test for biased strand distribution p-value = 6.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.77e-01

Only 100 of 193 total aligned reads displayed.

ATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATATGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTT  >  USA300TCH1516_ALE/1002112‑1002285

aTTGTGAAATTTTACGATTTAGAAGATGTTATTAATTATTCATATCATAGCAATCCAATAACTATAGAGTTTGAATATTTGTTATATAggg                                                                                     <  2:2571391/91‑1 (MQ=255)

 ttGTGAAATTTAACGATTTAGAAGATGTTATAAATTATGCACATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTCTTATATAGGGt                                                                                    >  2:2360072/1‑91 (MQ=255)

 ttGTCAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGt                                                                                    <  1:2584945/91‑1 (MQ=255)

    tgAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGa                                                                                 <  1:264704/91‑1 (MQ=255)

      aaaTTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATg                                                                               >  2:421605/1‑91 (MQ=255)

       aaTTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATgg                                                                              <  1:1645387/91‑1 (MQ=255)

       aaTTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATgg                                                                              <  1:2148057/91‑1 (MQ=255)

        aTTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGt                                                                             <  2:928246/91‑1 (MQ=255)

        aTTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGt                                                                             >  1:2314577/1‑91 (MQ=255)

         tttAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTa                                                                            <  1:575456/91‑1 (MQ=255)

          ttAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTAc                                                                           <  1:1478981/91‑1 (MQ=255)

          ttAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTAc                                                                           <  1:1480278/91‑1 (MQ=255)

           tAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACt                                                                          <  2:1847802/91‑1 (MQ=255)

            aaCGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACtt                                                                         >  1:2985355/1‑91 (MQ=255)

            aaCGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACtt                                                                         <  1:2249582/91‑1 (MQ=255)

             aCGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACtta                                                                        >  2:358107/1‑91 (MQ=255)

             aCGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACtta                                                                        <  2:862602/91‑1 (MQ=255)

             aCGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACtta                                                                        >  1:2311695/1‑91 (MQ=255)

              cGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACttat                                                                       <  2:1747713/91‑1 (MQ=255)

              cGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACttat                                                                       <  2:3098219/91‑1 (MQ=255)

               gATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACttatt                                                                      >  1:1790431/1‑91 (MQ=255)

                 tttAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACttattat                                                                    >  1:641471/1‑91 (MQ=255)

                   tAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACttattatta                                                                  >  1:317392/1‑91 (MQ=255)

                     gaagaTGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATg                                                                <  1:1845738/91‑1 (MQ=255)

                      aagaTGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGc                                                               <  1:1731390/91‑1 (MQ=255)

                      aagaTGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGc                                                               >  2:1596115/1‑91 (MQ=255)

                      aagaTGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGc                                                               <  1:701038/91‑1 (MQ=255)

                       agaTGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCt                                                              <  2:2059040/91‑1 (MQ=255)

                        gaTGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTg                                                             >  1:1173883/1‑91 (MQ=255)

                        gaTGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTg                                                             <  1:760971/91‑1 (MQ=255)

                        gaTGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTg                                                             <  2:1800960/91‑1 (MQ=255)

                         aTGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGt                                                            <  1:94176/91‑1 (MQ=255)

                         aTGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGt                                                            >  1:2078716/1‑91 (MQ=255)

                          tGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGta                                                           <  1:1812803/91‑1 (MQ=255)

                            ttattaATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGtata                                                         >  1:36819/1‑91 (MQ=255)

                              attaattaTGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATAtt                                                       <  2:1341107/91‑1 (MQ=255)

                              attaattaTGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATAtt                                                       >  2:1155598/1‑91 (MQ=255)

                              attaattaTGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATAtt                                                       >  2:2411823/1‑91 (MQ=255)

                              attaactaTGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATAtt                                                       <  2:2508786/91‑1 (MQ=255)

                               ttaattaTGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATAttt                                                      <  1:2009763/91‑1 (MQ=255)

                                taattaTGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATAtttt                                                     >  2:941759/1‑91 (MQ=255)

                                 aattaTGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTg                                                    >  2:710057/1‑91 (MQ=255)

                                 aattaTGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTg                                                    <  2:2525276/91‑1 (MQ=255)

                                  attaTGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGa                                                   <  1:2025293/91‑1 (MQ=255)

                                   ttaTGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGAt                                                  <  1:721384/91‑1 (MQ=255)

                                    taTGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATa                                                 >  1:714950/1‑91 (MQ=255)

                                     aTGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAg                                                >  2:3091126/1‑91 (MQ=255)

                                      tGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGt                                               >  2:555309/1‑91 (MQ=255)

                                      tGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGt                                               <  2:1370842/91‑1 (MQ=255)

                                       gCATATCATAGCAATCCAATAACTACAGAGTTTGAGTATTTGTTATATAGGGTTGATGGTACTTATTATTATGTTGTATATTTTGATAGTc                                              <  2:199402/91‑1 (MQ=255)

                                          tatCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATg                                           >  2:105759/1‑91 (MQ=255)

                                          tatCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATg                                           <  2:1736961/91‑1 (MQ=255)

                                           atCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGt                                          >  2:2941799/1‑91 (MQ=255)

                                            tCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGtt                                         <  2:916071/91‑1 (MQ=255)

                                            tCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGtt                                         <  1:1417303/91‑1 (MQ=255)

                                             cATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTg                                        >  1:108658/1‑91 (MQ=255)

                                               tAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGAt                                      >  2:2875137/1‑91 (MQ=255)

                                                aGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATc                                     <  2:2748861/91‑1 (MQ=255)

                                                aGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATc                                     >  2:2913737/1‑91 (MQ=255)

                                                aGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATc                                     >  1:49520/1‑91 (MQ=255)

                                                aGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATc                                     >  1:2743239/1‑91 (MQ=255)

                                                 gCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCa                                    >  1:1114468/1‑91 (MQ=255)

                                                 gCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCa                                    >  1:758218/1‑91 (MQ=255)

                                                    aTCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCaaga                                 >  1:1597455/1‑91 (MQ=255)

                                                     tCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCaagaa                                <  1:1802240/91‑1 (MQ=255)

                                                     tCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCaagaa                                <  2:2253263/91‑1 (MQ=255)

                                                     tCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCaagaa                                <  2:2507518/91‑1 (MQ=255)

                                                      ccAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCaagaag                               <  2:780495/91‑1 (MQ=255)

                                                      ccAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCaagaag                               <  2:3032605/91‑1 (MQ=255)

                                                       cAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGt                              >  2:997334/1‑91 (MQ=255)

                                                       cAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGt                              <  2:1459330/91‑1 (MQ=255)

                                                       cAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGt                              <  2:692560/91‑1 (MQ=255)

                                                        aaTAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTc                             <  1:312929/91‑1 (MQ=255)

                                                        aaTAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGAACTTATAATTATTCTGTATATTTTGATAGTCATGTTGATCAAGAAGTc                             >  1:825437/1‑91 (MQ=255)

                                                          tAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCAt                           <  2:1665374/91‑1 (MQ=255)

                                                           aaCTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGCAGTCAtt                          >  2:1563035/1‑91 (MQ=255)

                                                           aaCTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCAtt                          >  1:1957522/1‑91 (MQ=255)

                                                            aCTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTa                         >  2:1975193/1‑91 (MQ=255)

                                                             cTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTaa                        <  1:9947/91‑1 (MQ=255)

                                                             cTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTaa                        <  2:410637/91‑1 (MQ=255)

                                                              tACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAAt                       >  2:1905176/1‑91 (MQ=255)

                                                              tACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAAt                       <  2:880119/91‑1 (MQ=255)

                                                               aCAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATg                      <  2:1576028/91‑1 (MQ=255)

                                                                cAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGa                     >  2:1027016/1‑91 (MQ=255)

                                                                cAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGa                     <  2:1859278/91‑1 (MQ=255)

                                                                 agagTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGAt                    <  1:2974017/91‑1 (MQ=255)

                                                                  gagTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATa                   <  1:801002/91‑1 (MQ=255)

                                                                     tttGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGtt                <  2:2224567/91‑1 (MQ=255)

                                                                       tGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTAc              >  2:635111/1‑91 (MQ=255)

                                                                         aaGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAg            >  1:2461939/1‑91 (MQ=255)

                                                                           gATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTc          >  1:2573166/1‑91 (MQ=255)

                                                                                gTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTg     <  2:821527/91‑1 (MQ=255)

                                                                                 ttATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGc    <  2:2311695/91‑1 (MQ=255)

                                                                                 ttATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGc    >  2:1422813/1‑91 (MQ=255)

                                                                                  tatataGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCt   >  2:686150/1‑91 (MQ=255)

                                                                                  tatataGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCt   <  2:36889/91‑1 (MQ=255)

                                                                                  tatataGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCt   >  2:1297512/1‑91 (MQ=255)

                                                                                  tatataGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCt   <  1:941759/91‑1 (MQ=255)

                                                                                   atatagGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCtt  >  2:2927761/1‑91 (MQ=255)

                                                                                   atatagGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTACtt  >  1:2494005/1‑91 (MQ=255)

ATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATATGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTT  >  USA300TCH1516_ALE/1002112‑1002285

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`