Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE111,5160CA25.0% 119.4 / 34.3 69Q196K (CAA→AAA) USA300HOU_RS00520putative lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (27/24);  new base A (8/9);  total (35/34)
Fisher's exact test for biased strand distribution p-value = 7.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.19e-01
Rejected as consensus: Frequency below/above cutoff threshold.

AAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATA  >  USA300TCH1516_ALE/111382‑111645
                                                                                                                                      |                                                                                                                                 
aaGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTc                                                                                                                                   >  2:554820/1‑135 (MQ=255)
       agaagaAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATAACa                                                                                                                            <  1:363862/135‑1 (MQ=255)
        gaagaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATGTATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCaa                                                                                                                           >  2:585204/1‑135 (MQ=255)
         aagaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAAt                                                                                                                          <  2:321854/135‑1 (MQ=255)
           gcaaaCATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTa                                                                                                                        >  1:120219/3‑135 (MQ=255)
                    aaaaaaGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAatgat                                                                                                               >  1:638709/1‑135 (MQ=255)
                     aaaaaGAAATCGAAAACTTTAAGTTTTTTGCGCATTATAGCTATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAatgatg                                                                                                              <  2:397085/135‑1 (MQ=255)
                     aaaaaGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAatgatg                                                                                                              <  1:192779/135‑1 (MQ=255)
                      aaaaGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTTAATATCAATTAACTAAtgatga                                                                                                             <  1:142422/135‑1 (MQ=255)
                       aaaGCAATCGAAAACATTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATgatgat                                                                                                            >  1:176303/1‑135 (MQ=255)
                       aaaGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATgatgat                                                                                                            >  2:261316/1‑135 (MQ=255)
                          gAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATtat                                                                                                         <  2:666775/135‑1 (MQ=255)
                             aTCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACAAATGATGATTATAAt                                                                                                      >  1:428916/1‑135 (MQ=255)
                             aTCGAAAACTTTAAGTTTTTTGCGAAATATAGCAATTTTAAAGATTTAATCACTATTAAAGATGGAGTTATATCATATAATCAAGAAGTGCAGAGTTATTCGGTTAAATATCAATTAACTAATGATTATTATAAt                                                                                                      >  2:381685/1‑135 (MQ=18)
                                 aaaaCTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTATCTAATGATGATTATAATGTaa                                                                                                  <  1:4754/135‑1 (MQ=255)
                                 aaaaCCTTAAGTTTTTTGCGCAATATAGAAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTaa                                                                                                  <  2:490620/135‑1 (MQ=255)
                                  aaaCTTTAAGTTTTTTTCGAAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTaaa                                                                                                 <  2:411367/135‑1 (MQ=255)
                                  aaaCTTTAAGTTTTTTGCGAAATATAGCAATTTTAAAGATTTAATGAACTATAACGATGGAGATCTATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTACTGATGATTATAATGTaaa                                                                                                 <  2:186194/135‑1 (MQ=18)
                                     cTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACa                                                                                              <  1:305248/135‑1 (MQ=255)
                                     cTTTAAGTTTTTTGCGCAATAGAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACa                                                                                              <  2:128695/135‑1 (MQ=255)
                                        tAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTCTACAGAAGAAGATATATCATATAATAAAGGGTTTACTATGCAGTCAGCACAATACCAATTAACTAATGATGATTATAATGTAAAACAAtt                                                                                           >  2:636710/1‑135 (MQ=21)
                                        tAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAAtt                                                                                           >  1:82918/1‑135 (MQ=255)
                                        tAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACACtt                                                                                           >  2:367592/1‑135 (MQ=255)
                                              ttttGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTCTAATGTAAAACAATTACgcaa                                                                                     <  2:359092/135‑4 (MQ=17)
                                                  gcgcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGa                                                                                 <  2:583881/135‑1 (MQ=255)
                                                    gcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGGGTTATTCGGCTCAATATCAATTAACTAATGCTGCTTATAATGTACACCCATTACGTTAAAGatc                                                                               >  1:599848/1‑134 (MQ=18)
                                                    gcAATATAGCAATTTTAAAGATTTAATGAATGATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGata                                                                               <  2:74240/135‑1 (MQ=14)
                                                       atatAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGa                                                                            <  2:215517/135‑1 (MQ=255)
                                                          taGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAAGTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATAGGatat                                                                         >  1:21636/1‑135 (MQ=14)
                                                           aGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATa                                                                        <  1:63678/135‑1 (MQ=255)
                                                           aGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGAAATATGATATATTGCGGAGTAGCCGAGTTATTCAGCTCAATATCAATTAAATAATGATAATTATAATGTAAAACAATTACGTAAAAGATATGACATa                                                                        <  1:486721/135‑1 (MQ=18)
                                                              aaTTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATAccg                                                                     <  2:499032/135‑2 (MQ=11)
                                                              aaTTTTAAAGATTTAAGGGATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTGATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGTTATTACATACca                                                                     <  2:33337/135‑1 (MQ=18)
                                                                ttttAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACcaac                                                                   <  2:638709/135‑1 (MQ=255)
                                                                ttttAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAACTCAATATCAATTAACTAATGATGATTATAATGTAAAACCATAACGTAAAAGATATGACATACcaac                                                                   >  2:4404/1‑135 (MQ=255)
                                                                ttttAAAGATTTAATGAATTATAAAGATGGAGATATATCAGATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACcaac                                                                   >  2:590935/1‑135 (MQ=255)
                                                                 tttAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACcgacg                                                                  <  2:466169/135‑2 (MQ=1)
                                                                 tttAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAATATATGACATACcaaca                                                                  <  2:310371/135‑1 (MQ=255)
                                                                 tttAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACcaaaa                                                                  >  2:606335/1‑133 (MQ=255)
                                                                 tttAAAGATTTAATGAATTATAAAGATGAAAATATATCATATAATCCAGAGGTACCGAGTTATTCGGCTAAATATCAATTAACTAATTATGAATATAATTAAAAACAATTACGCAAAAGATATGATATACcaacg                                                                  >  1:249710/1‑134 (MQ=11)
                                                                       gATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATaaa                                                            <  1:222322/135‑1 (MQ=255)
                                                                         tttAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGCTTATAATGTAAAACAATTACGTAAAAGATATGACATACCACAAAATAAAgc                                                          >  2:318397/1‑135 (MQ=255)
                                                                         tttAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAGACAATTACGTAAAAGATATGACATACCAGCAAATAAAgc                                                          >  2:445241/1‑135 (MQ=255)
                                                                          ttAATGAATTATAAAGATGGAGATACATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAgcg                                                         >  2:350320/1‑135 (MQ=255)
                                                                              tGAATTATAAAGATTGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAATCCATTACTTAAAAGATATGACATACCAACAAATAAAGCGCCGa                                                     <  1:620202/135‑1 (MQ=255)
                                                                              tGAATTATAAAAATGGAGATATATCATAGAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGa                                                     <  2:655770/135‑1 (MQ=255)
                                                                                aactataAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACTAACAAATAAAGCGCCGAAg                                                   <  1:161825/132‑1 (MQ=255)
                                                                                 aTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGc                                                  <  2:399482/135‑1 (MQ=255)
                                                                                 aTTATAAAGATGGAGATACAGCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGc                                                  <  2:681097/135‑1 (MQ=255)
                                                                                  ttATAAATATGGAGATATATCATATAATCCAGAAGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATTACATACCAACTAATAAAGCGCCGAAGCt                                                 <  1:583866/135‑1 (MQ=255)
                                                                                      aaaGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATATCAACAAATAAAGCGCCGAAGCtgttg                                             <  1:136827/135‑1 (MQ=255)
                                                                                      aaaGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCtgttg                                             <  1:499526/135‑1 (MQ=255)
                                                                                      aaaGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCtgttg                                             <  1:566331/135‑1 (MQ=255)
                                                                                      aaaGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCtgttg                                             >  2:531681/1‑135 (MQ=255)
                                                                                      aaaGATGGAGATATATCATATAATCCAGAGGTACCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCtgttg                                             >  1:658260/1‑135 (MQ=255)
                                                                                              agaTATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAgg                                     >  2:425363/1‑135 (MQ=255)
                                                                                                     tCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAggg                              >  2:71148/1‑135 (MQ=255)
                                                                                                     tCATATAATCCAGAGGTGCCGAGATATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGATACAggg                              >  1:540768/1‑135 (MQ=255)
                                                                                                         ataATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAAtt                          >  2:569625/1‑135 (MQ=255)
                                                                                                         ataATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAAtt                          >  2:476090/1‑135 (MQ=255)
                                                                                                          taATCCAGAGGTGCCGAGTTATTCAGCTAAATATAAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATCACATATCCACAAATAAAGCGCCGAAGCGGTTGTTGAAAGGTAGAGGGAAttt                         >  1:31314/1‑135 (MQ=14)
                                                                                                                agagGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATAAAGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAgg                   >  1:334505/1‑135 (MQ=255)
                                                                                                                   ggTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTAAAGGGAATTTAAAAGGTtc                >  2:279234/1‑135 (MQ=255)
                                                                                                                     tGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtcat              <  2:176303/135‑1 (MQ=255)
                                                                                                                     tGCCGAGTTATTCAGCTCAATATCAATTAACTAATACTGATTATAATGTAAAAAAATTAAGTAAAAGATATAAAATAAAGACAAAGCAAGAGCCGAACCTGTTGTTGAAAAGTAACGCGCATATAAAAGGTtcat              >  2:277599/1‑135 (MQ=255)
                                                                                                                        cGAGTTATTCAGCTCAATATCAATAAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAg           >  1:12392/1‑135 (MQ=255)
                                                                                                                           gTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGATTCATCAGTTg        >  2:652855/1‑135 (MQ=255)
                                                                                                                            ttATTCAGCTCAATATCAAATAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTgg       >  1:489850/1‑135 (MQ=255)
                                                                                                                                 cAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGAtata  >  1:220917/1‑135 (MQ=255)
                                                                                                                                 cAGCTCAATATCAATTAACTAATGATAAAAAAAAAGTAAAACAATAACGAAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGATGGAtata  >  1:222156/1‑135 (MQ=14)
                                                                                                                                      |                                                                                                                                 
AAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATA  >  USA300TCH1516_ALE/111382‑111645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑