Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	584,121	G→T	intergenic (+205/‑67)	rplA → / → rplJ	50S ribosomal protein L1/50S ribosomal protein L10

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	584,121	0	G	T	100.0%	248.8 / NA	79	intergenic (+205/‑67)	rplA/rplJ	50S ribosomal protein L1/50S ribosomal protein L10
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (45/34);  total (45/34)

CCAAGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTG‑AACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCGTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCA  >  USA300TCH1516_ALE/583991‑584247                                                                                                                                    |                                                                                                                               ccAGGTTATATTACTTATTGTGATTATTTTAACTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGTGACAACATCAT‑TATGATCTTTCAAGCACTTTTTGCCTTggg                                                                                                                            >  2:108179/1‑135 (MQ=255)   aaGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTG‑AACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTa                                                                                                                          <  2:268248/135‑1 (MQ=255)    atttATATTACTTATTGAGATTCTTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTTACTTG‑AACGTGATGTTCTATTATCTTTCAAGCACTTTTTGCCTTGGGTAg                                                                                                                         <  1:505915/133‑1 (MQ=255)    aGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTG‑AACGTGATGATCTATGTTCTTTCAAGCACTTTTTGCCTTGGGTAg                                                                                                                         <  1:636614/135‑1 (MQ=255)    aGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTG‑AACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAg                                                                                                                         <  1:326285/135‑1 (MQ=255)     gTTATATTATTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCGGCCGAGGCTAAAATTGACTTG‑AACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGa                                                                                                                        <  2:198625/135‑1 (MQ=255)      ttATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTG‑TACGTGATGATCTATGATCTTTCAATCACTTTTTGCCTTGGGTAGaa                                                                                                                       <  2:433018/135‑1 (MQ=255)      ttATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTG‑AACGTTATTATCTATGATCTTTTAAGCTTTTTTTGCCTTGGGTAGaa                                                                                                                       <  1:151015/135‑1 (MQ=255)      ttATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTG‑AACGTGATGATCTATGATCTTTTAAGCACTTTTTGCCTTGGGTAGaa                                                                                                                       <  1:86877/135‑1 (MQ=255)        aaatTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTG‑AACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAg                                                                                                                     <  2:168704/133‑1 (MQ=255)              cTTATTGTGATTATTTTACCTAAAACAGTAGGAGATATTTATAACTTAAAATTTATCCTGCCGAGCCTAAAATTGACTTG‑AACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCttt                                                                                                               >  1:129211/1‑135 (MQ=255)                    gtgATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTG‑AACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTttat                                                                                                         >  2:424830/1‑135 (MQ=255)                    gtgATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTAGCCTGCCGAGGCTAAAATTGACTTG‑AACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTttat                                                                                                         <  2:428288/135‑1 (MQ=255)                     tgATTATTTTACCTACGACTGTACGAGCTAGTTAGAACTTTAATTTTATTCTGCCGAGGCTAAAATTGACTTG‑AACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTttatt                                                                                                        <  1:448442/135‑1 (MQ=255)                        ttattTTACCTCAGTCAGTAGGAGTTATTTATACCTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTG‑AACGTGATGTGCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAAt                                                                                                     <  2:110037/135‑1 (MQ=255)                        ttattTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTG‑AACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAAt                                                                                                     <  2:425005/135‑1 (MQ=255)                         tattTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTG‑AACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAAtt                                                                                                    >  1:402334/1‑135 (MQ=255)                            tttACCGAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTG‑AACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTa                                                                                                 <  2:492311/135‑1 (MQ=255)                              tACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTG‑AACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTaaa                                                                                               >  1:197542/1‑135 (MQ=255)                                ccTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTG‑AACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTaaaaa                                                                                             <  1:11698/135‑1 (MQ=255)                                  tAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTG‑AACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTTTTTTTTTTaaaaaaa                                                                                           >  2:274642/1‑135 (MQ=255)                                  caaGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTG‑AACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAACGCGTCATCTTTTTTAATTTTaaaaaaa                                                                                           <  2:114314/134‑1 (MQ=255)                                     gccAGTAGGAGTTATTTATAACTTAAAATTTATCATGCCGAGGCTAAAATTGACTTG‑AACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCa                                                                                        >  1:161036/3‑135 (MQ=255)                                      aCAGTAGTAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTG‑AACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCAc                                                                                       <  2:35543/135‑1 (MQ=255)                                      aCAGTAGGAGTTATTTATAACTTAAAATTTATCCTTCCGAGGCTAAAATTGACTTG‑AACGTGATGATCTATGATCTTTCAAGCACTTTTTTCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCAc                                                                                       <  1:581357/135‑1 (MQ=255)                                      aCAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTG‑AACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCAc                                                                                       <  1:141159/135‑1 (MQ=255)                                         gTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTT‑AACGTGATGATCTATGATTTTTCAAGCACTTTTTGTCTTTGGTAGAAAGTGCTTTTTTTTTTAATTTTAAAAAAAGCACCaa                                                                                    <  1:134226/135‑1 (MQ=255)                                         gTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTG‑AACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCaa                                                                                    <  1:387471/135‑1 (MQ=255)                                         gTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTG‑AACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCaa                                                                                    >  1:510967/1‑135 (MQ=255)                                              aatgatttatAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTG‑AACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAAtt                                                                               <  2:467937/131‑1 (MQ=255)                                              aGTTATTTATAACTTAAAATTTATCCTGCCGCGGCTAAAATTGACTTG‑AACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATCTTAAAAAAAGCACCAACAAtt                                                                               >  2:133002/1‑135 (MQ=255)                                                  atttatAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTG‑AACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTaaa                                                                           >  1:640605/1‑135 (MQ=255)                                                  atttatAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTG‑AACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTaaa                                                                           >  2:536099/1‑135 (MQ=255)                                                  atttatAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTG‑AACGTGATGATCTATGATCTTTCAAGCACTTTTCGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTaaa                                                                           >  2:386925/1‑135 (MQ=255)                                                    ttatAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTG‑AACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATg                                                                         >  1:384406/1‑135 (MQ=255)                                                          cTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTG‑AACGTGATGATCTATGATCTTTCAAGAAATTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGgtg                                                                   >  2:295035/1‑135 (MQ=255)                                                             aaaaTTTAGTCTGCCGAGGCTAAAATTGACTTG‑AACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCt                                                                <  2:601002/135‑1 (MQ=255)                                                                   tATCCTGCCGAGGCTAAAATTGACTTG‑AACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTCCTTTTAAAAAAAACACCAAAAATTTAAATGGAGGTGTCTGAATGt                                                          <  1:619795/135‑1 (MQ=255)                                                                   tATCCTGCCGAGGCTAAAATTGACTTG‑AACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTCAATGGCGGAGTCTGAATGt                                                          >  2:674349/1‑135 (MQ=255)                                                                    aTCCTGCCGAGGCTAAAATTGACTTG‑AACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTTTGAATGTc                                                         >  2:292677/1‑135 (MQ=255)                                                                    aTCCTGCCGAGGCTAAAATTGACTTG‑AACATGATGAACTATGATCTTTCAAGCACTTTTTGCCTTGGGCAGAAAGTGCTTTTTTTAGTAATTTTAAAAAAAGCACAAAAAGTTGAAATGGAGATGTCGAAATGTc                                                         >  1:264157/1‑135 (MQ=255)                                                                       cTGCCGAGGCTAAAATTGACTTG‑AACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGc                                                      >  1:159880/1‑135 (MQ=255)                                                                       cTGCCGAGGCTAAAATTGACTTG‑AACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGc                                                      >  1:600987/1‑135 (MQ=255)                                                                         gCCGAGGCTAAAATTGACTTG‑AACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGCGGTGTCTGAATGTCTGCTa                                                    >  2:165748/1‑135 (MQ=255)                                                                         gCCGAGGCTAAAATTGACTTG‑AACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTa                                                    >  1:186731/1‑135 (MQ=255)                                                                         gCCGAGGCTAAAATTGACTTG‑AACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTa                                                    >  2:45245/1‑135 (MQ=255)                                                                         gCCGAGGCTAAAATTAACAAA‑AACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTa                                                    >  1:495070/1‑135 (MQ=255)                                                                            gAGGCTAAAATTGACTTG‑AACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGTAGGTGTCTGAATGTCTGCTATCa                                                 >  1:302011/1‑135 (MQ=255)                                                                             tggCTAAAAGTGACTTG‑AACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCAt                                                <  2:466448/134‑1 (MQ=255)                                                                              ggCTAAAATTGACTTG‑AACGTAATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCAtt                                               >  1:527016/1‑135 (MQ=255)                                                                              ggCTAAAATTGACTTA‑AACATGATAATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAGAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCAtt                                               >  1:473611/1‑135 (MQ=255)                                                                               gCTAAAATTGACTTG‑AACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTTTTATTTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTg                                              >  2:119937/1‑135 (MQ=255)                                                                                  aaaaTTGACTTG‑AACGTGATGATCTATGATCTTTCAAGCACTTTTTGTCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGAACCAAAAATTTAAATGGATGTGTCTGAATGTCATCTAGCATTGAAg                                           >  2:165310/1‑135 (MQ=255)                                                                                  aaaaTTGACTTG‑AACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAg                                           >  2:447580/1‑135 (MQ=255)                                                                                   aaaTTGACTTG‑AACGTGATGATCTATGATCTTTCAAGCACTTTTTGACTTGGGTAGAAAGTGCTTTTTTTCTTAGCTGTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTATGCTATCATTGAAGc                                          >  1:453913/1‑135 (MQ=255)                                                                                   aaaTTGACTTA‑AACGTGATGATATATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGc                                          >  1:466982/1‑135 (MQ=255)                                                                                    aaTTGACTTG‑AACGTGCAGATACATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCt                                         >  2:255296/1‑135 (MQ=255)                                                                                    aaTTGACTTG‑AACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCt                                         >  2:233281/1‑135 (MQ=255)                                                                                     cttGCCTTG‑AACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGGATCATTTGGGTAGATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTa                                        >  1:165511/2‑135 (MQ=255)                                                                                        gccTTG‑AACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTaaaa                                     >  1:222186/3‑135 (MQ=255)                                                                                        gACTTG‑AACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAACACCAAAAATTTAAATGGAGCTGTCTGAATGTCTGCTATCATTGAAGCTaaaa                                     >  2:454800/1‑135 (MQ=255)                                                                                            tG‑AACGTGATGATCTATGATCTTTCGAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCACTGAAGCTAAAAaaca                                 <  1:348526/135‑1 (MQ=255)                                                                                            tG‑AACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAaaca                                 <  1:302654/135‑1 (MQ=255)                                                                                             ggatggTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGATGTGTCTGTATTTCTGTTATCATTGAAGCTAAAAaaca                                 <  1:81993/130‑1 (MQ=255)                                                                                                  gTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAg                            <  1:449988/135‑1 (MQ=255)                                                                                                     atgatctatgctctTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTtg                         >  1:274582/1‑135 (MQ=255)                                                                                                     atgatctatgatctTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAATCTGAAAAACAACAAGtta                         >  2:100032/1‑134 (MQ=255)                                                                                                     atgatatatgatctTTCAAGCACTTTTTGGCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTtg                         >  2:595439/1‑135 (MQ=255)                                                                                                         tctatgatctTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTggatga                     <  1:642155/135‑1 (MQ=255)                                                                                                            atgatctTTCAAGCACTTTTTTTTTTGTTTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGACTGTCTGCTATCATTGAAGCTAAAAAACAACTACTtgattatat                  >  2:104715/1‑130 (MQ=255)                                                                                                               atctTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGc               <  2:197542/135‑1 (MQ=255)                                                                                                                 ctTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTg             <  2:402334/135‑1 (MQ=255)                                                                                                                       aGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTAc       >  1:462390/1‑135 (MQ=255)                                                                                                                       aGCACTTTTTGCCTTGGATAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTAc       >  1:575253/1‑135 (MQ=255)                                                                                                                         cACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTTAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTa     >  2:122812/1‑135 (MQ=255)                                                                                                                         cACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTa     <  2:640605/135‑1 (MQ=255)                                                                                                                         cACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGAACTa     <  1:386925/135‑1 (MQ=255)                                                                                                                            tttttGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCa  >  1:20614/1‑135 (MQ=255)                                                                                                                            tttttGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCa  >  1:80566/1‑135 (MQ=255)                                                                                                                                    |                                                                                                                               CCAAGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTG‑AACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCGTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGGTACTATCA  >  USA300TCH1516_ALE/583991‑584247

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑