| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 297,176 | C→T | 6.1% | S337L (TCA→TTA) | tarK → | Teichoic acid ribitol‑phosphate polymerase TarK |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 297,176 | 0 | C | T | 6.1% | 195.7 / 2.6 | 68 | S337L (TCA→TTA) | tarK | Teichoic acid ribitol‑phosphate polymerase TarK |
| Reads supporting (aligned to +/- strand): ref base C (30/33); new base T (2/2); total (32/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.00e-01 | |||||||||||
CTTTAGACCATCACAAGAAATTATTCAAGTGTGGCATGCTGTTGGTGCCTTTAAAACAGTTGGCTTTAGTCGTACAGGTAAAAAAGGTGGTCCGTTTATCGATTCATTAAACCATCGTAGTTACACGAAAGCATATGTTTCATCAGAAACCGATATTCCATTTTATGCTGAAGCATTTGGAATTAGAGAAGAAAATGTTGTACCAACAGGTGTACCACGTACTGATGTACTATTTGATGAAGCTTATGCAACACAAATTAAACAAGAGATGGAAGATGAAT > USA300TCH1516_ALE/297036‑297316 | cTTTAGACCATCACAAGAAATTATTCAAGTGTGGCATGCTGTGGGTGCCTTTAAAACAGTTGGCTTTAGTCGCACATGTAAAAAAGGTGGTCCGTTTATCGATTCATTAAACCATCGTAGTTACACGAAAGCATATGTTtc < 1:105406/141‑1 (MQ=255) ttAGACCATCACAAGAACTTATTCAAGTGTGGCATGCTGGTGGTGCCTTTAAAACAGTTGGCTTTAGTCGTACAGGTAAAAAAGGTGGTCCGTTTATCGATTCATTAAACCATCGTAGTTACACGAAAGCATATGTTtcat < 2:640789/141‑1 (MQ=255) ttAGACCATCACAAGAAATTATTCAAGTGTGGCATGCTGTTGGTGCCTTTAAAACAGTTGGCTTTAGTCGTACAGGTAAAAAAGGTGGTCCGTTTATCGATTCATTAAACCATCGTAGTTACACGAAAGGAGATGTTtcat > 2:369387/1‑141 (MQ=255) cATCACAAGAAATTATTCAAGTGTGGCATGCTGTTGGTGCCTTTAAAACAGTTGGCTTTAGTCGTACAGGTAAAAAAGGTGGTCCGTTTATCGATTCATTAAACCATCGTAGTTACACGAAAGCATATGTTTCATCAGaaa > 2:483400/1‑141 (MQ=255) accaGAAAGTATTCAAGTGTGGCATGCTGTTGGTGCCTTTAAAACAGTTGGCTTTAGTCGTACAGGTAAAAAAGGTGGTCCGTTTATCTATTCATTAAACCATCGTAGTTACACGAAAGCATATGTTTCATCAGAAACCGa < 2:20271/138‑1 (MQ=255) aGAAATTATTCAAGCGTGGCATGCTGTTGGTGCCTTTAAAACAGTTGGCTTTAGTCGTACAGGTAAAAAAGGTGGTCCGTTTATCGATTCATTAAACCATCGTAGTTACACGAAAGCATATGTTTCATCAGATACCGttat > 1:402667/1‑141 (MQ=255) cAAGTGTGGCATGCTGTTGTTGCCTTTAAAACAGTTGGCTTTAGTCGTACAGGTAAAAAAGGTGGTCCGTTTATCGATTCATTAAACCATCGTAGTTACACGAAAGCATATGTTTTATCAGAAACCGATATTCCATTTTAt > 2:199782/1‑141 (MQ=255) cAAGTGTGGCATGCTGTTGGTGCCTTTAAAACAGTTGGCTTTAGTCGTACAGGTAAAAAAGGTGGTCCGTTTATCGATTCATTAAACCATCGTAGTTACACGAAAGCATATGTTTTATCAGAAACCGATATTCCATTTTAt > 2:648918/1‑141 (MQ=255) cAAGTGTGGCATGCTGTTGGTGCCTTTAAAACAGTTGGCTTTAGTCGTACAGGTAAAAAAGGTGGTCCGTTTATCGATTCATTAAACCATCGTAGTTACACGAAAGCATATGTTTCATCAGAAACCGATATTCCATTTTAt > 1:615029/1‑141 (MQ=255) aaGTGTGGCATGCTGTTGGTGCCTTTAAAACAGTTGGCTTTAGTCGTACAGGTAAAAAAGGTGGTCCGTTTATCGATTCATTAAACCATCGTAGTTACACGAAAGCATATGTTTCATCAGAAACCGATATTCCATTTTATg < 1:135829/141‑1 (MQ=255) aaGTGTGGCATGCTGTTGGGCCTTTTAAAACAGTTGTCTTTAGTCGTACAGGTAAAAAAGGTGGTCCGTTTATCGATTTATTAAACCATCGTAGTTACACGAAAGCATATGTTTCATCAGAAACCGATATTCCATTTTATg < 1:391370/141‑1 (MQ=255) ggCCTGCTGTTGGTGCCTTGAAAACAGTTGGCTTTAGTCGTACAGGTAAAAAAGGTGGTCCGTTTATCGATTCATTAAACCATCGTAGTTACACGAAAGCATATGTTTCATCAGAAACCGATATTCCATTTTATGCTGAAg < 1:196596/141‑1 (MQ=255) cATGCTGTTGGTGCCTTTAAAACAGTTGGCTTTAGTCGTACAGGTAAAAAAGGTGGTCCGTTTATCGATTCATTAAACCATCGTAGTTACACGAAAGCATATGTTTCATCAGAAACCGATATTCCATTTTATGCTGAAGCa > 2:427104/1‑141 (MQ=255) aTGCTGTTGGTGCCTTTAAAACAGTTGGCTTTAGTCGTACAGGTAAAAAAGGTGGTCCGTTTATCGATTCATTAAACCATCGTAGTTACACGAAAGCATATGTTTCATCAGAAACCGATATTCCATTTTATGCTGAAGCAt < 1:693764/141‑1 (MQ=255) aTGCTGTTCGTGCCTTTAAAACAGTTGGCTTTAGTCGTACAGGTAAAAAAGGTGGTCCGTTTATCGATTCATTAACCCATCGTAGTTACACGAAAGCATATGTTTCATCAGAAACCGATATTCCATTTTATGCTGAAGCAt < 1:193476/141‑1 (MQ=255) gCTGTTGGTGCCTTTAAAACAGTTGGCTTTAGTCGTACAGGTAAAAAAGGTGGTCCGTTTATCGATTCATTAAACCATCGTAGTTACACGAAAGCATATGTTTCATCAGAAACCGATATTCCATTTTATGCTGAAGCAttt < 2:513724/141‑1 (MQ=255) ttGGTGCCTTTAAAACAGTTGGCTTTAGTCGTACAGGTAAAAAAGGTGGTCCGTTTATCGATTCATTAAACCATCGTAGTTACACGAAAGCATATGTTTCATCAGAAACCGATATTCCATTTTATGCTGAAGCATTTGGaa < 2:120838/141‑1 (MQ=255) tGCCTTTAAAACAGTTGGCTTTAGTCGTACAGGTAAAAAAGGTGGTCCGTTTATCGATTCGTTAAACCATCGTAGTTACACGAAAACATATGTTTCATCAGAAACCGATATTCCATTTTATGCTGAAGCTTTTGGAATTag > 1:322409/1‑141 (MQ=255) tGCCTTTAAAACAGTTGGCTTTAGTCGTACAGGTAAAAAAGGTGGTCCGTTTATCGATTCATTAAACCATCGTAGTTACACGAAAGCATATGTTTCATCAGAAACCGATATTCCATTTTATGCTGAAGCATTTGGAATTag < 2:661847/141‑1 (MQ=255) ccTTTAAAACAGTTGGCTTTAGTCGTACAGGTAAAAAAGGTGGTCCGTTTATCGATTCATTAAACCATCGTAGTTACACGAAAGCATATGTTTCATCAGAAACCGATATTGACATCAATCCTGAAGCATTTGGAATTagag < 1:768636/141‑1 (MQ=21) tttAAAACAGTTGGCTTTAGTAGTACAGGTAAAAAAGGTGGTCCGTTAATCGATTCATTAAACCATCGTAGTTACACGAAAGCATATGTTTCATCAGACACCGACATTCCATTTTATGCTGAAGGATTTGGAATTatggaa > 2:564603/1‑136 (MQ=21) tAAAACAGTTGGCTTTAGTCGTACAGGTAAAAAAGGTGGTCCGTTTATCGATTCATTAAACCATCGTAGTTACACGAAAGCATATGTTTCATCAGAAACCGATATTCCATTTTATGCTGAAGCATTTGGAATTAGagaaga < 2:255454/141‑1 (MQ=255) aaaCAGTTGGCTTTAGTCGTACAGGTAAAAAAGGTGGTCCGTTTATCGATTCATTAAACCATCGTAGTTACACGAAAGCATATGTTTCATCAGAAACCGATATTCCATTTTATGCTGAAGCATTTGGAATTAGagaagaca > 2:237855/1‑139 (MQ=255) cAGTTGGCTTTAGTCGTACAGGTAAAAAAGGTGGTCCGTTTATCGATTCATTAAACCATCGTAGTTACACGAAAGCATATGTTTCATCAGAAACCGATATTCCATTTTATGCTGAAGCATTTGGAATTAGAGAAGAAAAtg < 1:306794/141‑1 (MQ=255) cAGTTGGCTTTAGTCGTACAGGTAAAAAAGGTGGTCCGTTTATCGATTCATTAAACCATCGTAGTTACACGAAAGCATATGTTTCATCAGAAACCGATATTCCATTTTATGCTGAAGCATTTGGAATTAGAGAAGAAAAtg < 2:122057/141‑1 (MQ=255) cAGTTGGCTTTAGTCGTACAGGTAAAAAAGGTGGTCCGTTTATCGATTCATTAAACCATCGTAGTTACACGAAAGCATATGTTTCATCAGAAACCGATATTCCATTTTATGCTGAAGCATTTGGAATTAGAGAAGAAAAtg > 1:91894/1‑141 (MQ=255) gtGGCTTTAGTCGTACAGGTAAAAAAGTTGGTCCGTTTATGGATTCATTAAACCATCGTAGTTACACGAAAGCATATGTTTCATCAGAAACCGATATTCCATTTTATGCTGAAGCATTTGGAATTAGAGAAGAAAAtgttg < 2:184254/140‑1 (MQ=255) ggCTTTAGTCGTACAGGTAACAAAGGTGGTCCGTTTATCGATTCATTAAACCATCGTAGTTACACGAAAGCATATGTTTCATCAGAAACCGATATTCCATTTTATGCTGAAGCATTTGGAATTAGAGAAGAAAATGTTGTa > 1:489079/1‑141 (MQ=255) cTTTAGTCGTACAGGTAAAAAAGGTGGTCCGTTTATCGATTCATTAAACCATCGTAGTTACACGAAAGCATATGTTTCATCAGAAACCGATATTCCATTTTATGCTGAAGCATTTGGAATTAGAGAAGAAAATGTTGTAcc > 1:411827/1‑141 (MQ=255) ttAGTCGTACAGGTAAAAAAGGTGGTCCGTTTATCGATTAATTAAACCATCGTAGTTACACGAAAGCATATGTTTCATCAGAAACCGATATTCCATTTTATGCTGAAGCATTTGGAATTAGAGAAGAAAATGTTGTACCaa > 2:62913/1‑141 (MQ=255) aCAGGTAAAAAAGGTGGTCCGTTTATCGCTTCATTAAACCATCGTAGTTACACGAAAGCAGATGTTTCATCAGAAACCGATGTTCCATTTTATGCTGACGCATTGGGAATAACAGACGAAAATGTTGTCCTAACACgtgtc > 2:271705/1‑140 (MQ=255) gTAAAAAAGGTGGTCCGTTTATCGATTCATTAAACCATCGTAGTTACACGAAAGCATATGTTTCATCAGAAACCGATATTCCATTTTATGCTGAAGCATTTGGAATTAGAGAAGAAAATGTTGTACCAACAGGTGTACCAc < 1:650161/141‑1 (MQ=255) aaaaaGGTGGTCCGTTTATCGATTCATTAAACCATCGTAGTTACACGAAAGCATATGTTTCATCAGAAACCGATATTCCATTTTATGCTGAAGCATTTGGAATTAGAGAAGAAAATGTTGTACCAACAGGTGTACCACGTa < 2:587523/141‑1 (MQ=255) aaaaGGTGGTCCGTTTATCGATTCATTAAACCATCGTAGTTACACGAAAGCATATGTTTCATCAGAAACCGATATTCCATTTTATGCTGAAGCATTTGGAATTAGAGAAGAAAATGTTGTACCAACAGGTGTGCCACGTAc > 1:405292/1‑141 (MQ=255) ggtggtCCGTTTATCGATTCGTTAAACCATCGTAGTTACACGAACGCATATGATTCATGAGAAACCGATATTCCATTTTATGCTGAAGCATTTGGAATTAGAGAAGAAAATGTTGTACCAACAGGTGTACCACGTACTGAt > 1:471916/1‑141 (MQ=255) tgggCCCTTTATCGATTCATTAAACCATCCTAGTTACACGAAAGCATATGTTACATCTGGCACCGATATTCCATTTTATGCTGAAGCATTTGGAATTAGAGAAGAAAATGTTGTACCAACAGGTGTACCACGTACTGATGt < 1:366742/141‑1 (MQ=255) tCCGTTTATCGATTCATTAAACCATCGTAGTTACACGAAAGCATATGTTTCATCAGAAACCGATATTCCATTTTATGCTGAAGCATTTGGAATTAGAGAAGAAAATGTTGTACCAACAGGTGTACCACGTACTGATGTACt < 2:667708/141‑1 (MQ=255) ttATCTATTTATTAAACCATCTTAGTTACACGAAAGCATATGTTTCATCAGAAACCGATATTCCATTTTATGCTGAAGCATTTGGAATTAGAGAAGAAAATGTTGTACCACCATGTGTACCACGTACTGATGTACTATTtg < 2:354387/141‑1 (MQ=25) aTCGATTCATTAAACCATCGTAGTTACACGAAAGCATATGTTTCATCAGAAACCGATATTCCATTTTATGCTGAAGCATTTGGAATTAGAGAAGAAAATGTTGTACAAACAGGTGTACAACGTAATGATGTACTATTtgtt > 1:544177/1‑139 (MQ=255) aTTCATTAAACCATCGTAGTTACACGAAAGCATATGTTTCATCAGAAACCGATATTCCATTTTATGCTGAAGCATTTGGAATTAGAGAAGAAAATGTTGTACCAACAGGTGTACCACGTACTGATGTACTATTTGATGAAg < 1:262253/141‑1 (MQ=255) aaaCCATCGTAGTTACACGAAAGCATATGTTTCATCAGAAACCGATATTCCATTTTATGCTGAAGCATTTGGAATTAGAGAAGAAAATGTTGTACCAACAGGTGTACCACGTACTGATGTACTATTTGCTGAAGGTTATGc > 2:117599/1‑141 (MQ=255) aaaCCATCGTAGTTACACGAAAGCATATGTTTCATCAGAAACCGATATTCCATTTTATGCTGAAGCATTTGGAATTAGAGAAGAAAATGTTGTACCAACAGGTGTACCACGTACTGATGTACTATTTGATGAAGCTTATGc < 1:698699/141‑1 (MQ=255) aaCCATCGTAGTTACACGAAAGCATATGTTTCATCAGAAACCGATATTCCATTTTATGCTGAAGCATTTGGAATTAGAGAAGAAAATGTTGTACCAACAGGTGTACCACGTACTGATGTACTATTTGATGAAGCTTATGCa < 2:489079/141‑1 (MQ=255) aCCATCGTAGTTACACGAAAGCATATGTTTTATCAGAAACCGATATTCCATTTTATGCTGAAGCATTTGGAATTAGAGAAGAAAATGTTGTACCAACAGGTGTACCACGTACTGATGTACTATTTGATGAAGCTTATGCaa < 1:199782/141‑1 (MQ=255) aCCATCGTAGTTACACGAAAGCATATGTTTTATCAGAAACCGATATTCCATTTTATGCTGAAGCATTTGGAATTAGAGAAGAAAATGTTGTACCAACAGGTGTACCACGTACTGATGTACTATTTGATGAAGCTTATGCaa < 1:648918/141‑1 (MQ=255) aCCATCGTAGTTACACGAAAGCATATGTTTCATCAGAAACCGATATTCCATTTTATGCTGAAGCATTTGGAATTAGAGAAGAAAATGTTGTACCAACAGGTGTACCACGTACTGATGTACTATTTGATGAAGCTTATGCaa < 2:592154/141‑1 (MQ=255) aCCATCGTAGTTACACGAAAGCATATGTTTCATCAGAAACCGATATTCCATTTTATGCTGAAGCATTTGGAATTAGAGAAGAAAATGTTGTACCAACAGGTGTACCACGTACTGATGTACTATTTGATGAAGCTTATGCaa > 2:484330/1‑141 (MQ=255) aCCATCGTAGTTACACGAAAGCATATGTTTCATCAGAAACCGATATTCCATTTTATGCTGAAGCATTTGGAATTAGAGAAGAAAATGTTGTACCAACAGGTGTACCACGTACTGATGTACTATTTGATAAAGCTTATGCaa > 1:147595/1‑141 (MQ=255) tCGTAGTTACACGAAAGCATATGTTTCATCAGAAACCGATATTCCATTTTATGCTGAAGCATTTGGAATTAGAGAAGAAAATGTTGTACCAACAGGTGTACCACGTACTGATGTACTATTTGATGAAGCTAATGCAAcaca > 2:441633/1‑141 (MQ=255) cGTAGTTACACGAAAGCATATGTTTCATCAGAAACCGATATTCCATTTTATGCTGAAGCATTTGGAATTAGAGAAGAAAATGTTGTACCAACAGGTGTACCACGTACTGATGTACTATTTGATGAAGCTTATGCAACACaa > 1:250635/1‑141 (MQ=255) gTAGTTACACGAAAGCATATGTTTCATCAGAAACCGATATTCCATTTTATGCTGAAGCATTTGGAATTAGAGAAGAAAATGTTGTACCAACAGGTGTACCACGTACTGCTGTACTATTTGATGACTGTTATGCCTCACaaa > 2:726681/1‑141 (MQ=255) tAGTTACACGAAAGCATATGTTTCATCAGAAACCGATATTCCATTTTATGCTGAAGCATTTGGAATTAGAGAAGAAAATGTTGTACCAACAGGTGTACCACGTACTGATGTACTATTTGGTGAAGCTTATGCAACACAAAt > 1:197129/1‑141 (MQ=255) ttACACGAAAGCATATGTTTCATCAGAAACCGATATTCCATTTTATGCTGAAGCATTTGGAATTAGAGAAGAAAATGTTGTACCAACAGGTGTACCACGTACTGATGTACTATTTGATGAAGCTTATGCAACACAAATTaa > 1:588504/1‑141 (MQ=255) ttACACGAAAGCATATGTTTCATCAGAAACCGATATTCCATTTTATGCTGAAGCATTTGGAATTAGAGAAGAAAATGTTGTACCAACAGGTGTACCACGTACTGATGTACTATTTGATGAAGCTTATGCAACACAAATTaa > 2:374974/1‑141 (MQ=255) tgacaacaaaGCATATGTTTCATCAGAAACGGATATTCCATTTTATGCTGAAGCATTTTGAATTAGAGAAGAAAATGTTGTACCAACAGGCGTACCACGTACTGATGTACTATTTGATGAAGCTTATGCAACACAAATTaa < 2:93500/134‑1 (MQ=21) tACACGAAAGTATATGTTTCATCAGAAACCGATATTCCATTTTATGCTGAAGCATTTGGAATTAGAGAAGAAAATGTTGTACCAACAGGTGTACCACGTACTGATGTACTATTTGATGAAGCTTATGCAACACAAATTaaa < 2:456816/141‑1 (MQ=255) tACACGAAAGCATATGTTTCATCAGAAACCGATATTCCATTTTATGCTGACGCATCTGGAATTAGAGACGAAACGCTTGTACCAACAGGTGTACCACGTACTGATGTACTAGTTGATGAAGCTTATGCCACACAAATCaaa > 1:763914/1‑141 (MQ=255) tACACGAAAGCATATGTTTCATCAGAAACCGATATTCCATTTTATGCTGAAGCATTTGGAATTAGAGAAGAAAATGTTGTACCAACAGGTGTACCACGTACTGATGTACTATTTGATGAAGCTTATGCAACACAAATTaaa < 2:541405/141‑1 (MQ=255) gAAAGCGTATGTTTCATCAGAAACCGATATTGCATTTTACGCTGAAGCATTTGGAATTAGAGAAGAAAATGTTGTACCAACAGGTGTACCACGTACTGATGTACTATTTGATGAAGCTTATGCAACACAAATTAAACAaga < 2:21978/141‑1 (MQ=255) gAAAGCATATGTTTCATCAGAAACCGATATTCCATTTTATGCTGAAGCATTTGGAATTAGAGAAGAAAATGTTGTACCAACAGGTGTACCACGTACTGATGTACTATTTGATGAAGCTTATGCAACACAAATTAAACAaga < 2:649851/141‑1 (MQ=255) gAAAGCATATGTTTCATCAGAAACCGATATTCCATTTTATGCTGAAGCATTTGGAATTAGAGAAGAAAATGTTGTACCAACAGGTGTACCACGTACTGATGTACTATTTGATGAAGCTTATGCAACACAAATTAAACAaga < 2:662369/141‑1 (MQ=255) aGCATATGTTTCATCAGAAACCGATATTCCATTTTATGCTGAAGCATTTGGAATTAGAGAAGAAAATGTTGTACCAACAGGTGTACCACGTACTGATGTACTATTTGATGAAGCTTATGCAACACAAATTAAACAAgagag > 2:253478/1‑140 (MQ=255) aGCATATGTTTCATCAGAAACCGATATTCCATTTTATGCTGAAGCATTTGGAATTAGAGAAGAAAATGTTGTACCAACAGGTGTACCACGTACTGATGTACTATTTGATGAAGCTTATGCAACACAAATTAAACAAGAGAt < 1:89649/141‑1 (MQ=255) gatatGTTTCATCAGGAACCGATATTCCATTTTATGCTGAAGCATTTGGAATTAGAGAAGAAAATGTTGTACCAACAGGTGTACCACGTACTGATGTACTATTTGATGAAGCTTATGCAACACAAATTAAACAAGAGATgg < 1:600595/140‑1 (MQ=255) cATATGTTTCATCAGAAACCGATATTCCATTTTATGCTGAAGCATTTGGAATTAGAGAAGAAAATGTTGTACCAACAGGTGTACCACGTACTGATGTACTATTTGATGAAGCTTATGCAACACAAATTAAACAAGAGATgg > 1:777426/1‑141 (MQ=255) tatGTTTCATCAGAAACCGATATTCCATTTTATGCTGAAGCATTTGGAATTAGAGAAGAAAATGTTGTACCAACAGGTGTACCACGTACTGATGTACTATTTGATGAAGCTTATGCAACACAAATTAAACAAGAGATGGaa > 2:485935/1‑141 (MQ=255) tatGTTTCATCAGAAACCGATATTCCATTTTATGCTGAAGCATTTGGAATTAGAGAAGAAAATGTTGTACCAACAGGTGTACCACGTACTGATGTACTATTTGATGAAGCTTATGCAACACAAATAAAACAAGAGATGGaa > 2:698677/1‑141 (MQ=255) tatGGTTCATCAGAAACCGATATTCCATTTTATGCTGAAGCATTTGGAATTAGAGAAGAAAATGTTGTACCAACAGGTGTACCACGTACTGATGTACTATTTGATGAAGCTTATGCAACACAAATTAAACAAGAGATGGaa > 2:91590/1‑141 (MQ=255) atGTTTCATCAGAAACCGATATTCCATTTTATGCTGAAGCATTTGGAATTAGAGAAGAAAATGTTGTACCAACAGGTGTACCACGTACTGATGTACTATTTGATGAAGCTTATGCAACACAAATTAAACAAGAGATGGAAg < 1:240204/141‑1 (MQ=255) tGTTTGATCAGAAACCGATATTCCATTTTATGCTGAAGCATTTGGAATTAGAGAAGAACATGTTGTACCAACAGGTGTACCACGTACTGATGTACTATTTGATGAAGCTTATGCAACACAAATTAAACAAGAGATGGAAGa < 1:62913/141‑1 (MQ=255) tttCATCAGAAACCGATATTCCATTTTATGCTGAAGCATTTGGAATTAGAGAAGAAAATGTTGTACCAACAGGTGTACCACGTACTGATGTACTATTTGATGAAGCTTATGCAACACAAATTAAACAAGAGATGGAAGATg < 2:692064/141‑1 (MQ=255) catcaGAAACCGATATTCCATTTTATGCTGAAGCATTTGGAATTAGAGAAGAAAATGTTGTACAAACAGGTGTACCACGTACTGATGTACTATTTGATGAAGATTATGCAGCAAAAATTAAACAAGAGAGGGAAGATGAAt > 2:214022/1‑141 (MQ=21) | CTTTAGACCATCACAAGAAATTATTCAAGTGTGGCATGCTGTTGGTGCCTTTAAAACAGTTGGCTTTAGTCGTACAGGTAAAAAAGGTGGTCCGTTTATCGATTCATTAAACCATCGTAGTTACACGAAAGCATATGTTTCATCAGAAACCGATATTCCATTTTATGCTGAAGCATTTGGAATTAGAGAAGAAAATGTTGTACCAACAGGTGTACCACGTACTGATGTACTATTTGATGAAGCTTATGCAACACAAATTAAACAAGAGATGGAAGATGAAT > USA300TCH1516_ALE/297036‑297316 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |