Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 478,502 C→A 100% D342E (GAC→GAA)  nuoL → NADH‑quinone oxidoreductase subunit L

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE478,5020CA100.0% 208.8 / ‑6.5 70D342E (GAC→GAA) nuoLNADH‑quinone oxidoreductase subunit L
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (23/46);  total (23/47)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTCAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTT‑TTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGACGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCTTGGAATCA  >  USA300TCH1516_ALE/478366‑478639
                                                                                                                                         |                                                                                                                                         
ttCAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTT‑TTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCtt                                                                                                                                       <  2:247545/141‑1 (MQ=255)
ttCAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTT‑TTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCtt                                                                                                                                       <  2:122059/141‑1 (MQ=255)
  cAATGTGCATTGGGTGTATATTCAGCTGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTT‑TTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTAt                                                                                                                                     <  1:758947/141‑1 (MQ=255)
  cAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTT‑TTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTAt                                                                                                                                     >  1:43793/1‑141 (MQ=255)
     tgtgCATTGGGTGTATATTCAGCAGCGATTATTAATTTAAGATAGCAAGGCATATGTAAAGCAACATTATTT‑TTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAACTTCTGGc                                                                                                                                  >  1:181700/1‑141 (MQ=255)
      gttgaTTGGGGGGATATTCAGCAGCGATTATTCATTTAATTTTGCACGGTATTTTTAAAGCAACATTATTT‑TTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCt                                                                                                                                 <  2:125662/137‑1 (MQ=255)
      gtccATTGGGTGTAAATTCAGCAGCGATTACTCATTTATTATTGCACCTTATTTTTAAAGCCACATTATTT‑TTTCAATCAGTTCCTATCGTGAAGCGATTCAAGATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCt                                                                                                                                 <  2:213758/138‑1 (MQ=255)
          aTTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTT‑TTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGAt                                                                                                                             <  1:19781/141‑1 (MQ=255)
            tGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTT‑TTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGGTTg                                                                                                                           >  1:546670/1‑141 (MQ=255)
            tGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTT‑TTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTg                                                                                                                           >  1:452340/1‑141 (MQ=255)
              ggTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTT‑TTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCCAAACAAGCATCTGCTAAAGAAGCTTCTGGCTGGATTGTc                                                                                                                         >  2:638997/1‑141 (MQ=255)
               gtgtTTATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTT‑TTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCa                                                                                                                        <  2:125123/141‑1 (MQ=255)
                 gtATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTT‑TTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATg                                                                                                                      >  1:212288/1‑141 (MQ=255)
                   atatTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTT‑TTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATggg                                                                                                                    >  1:438020/1‑141 (MQ=255)
                    tatTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTT‑TTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGa                                                                                                                   <  1:335653/141‑1 (MQ=255)
                        cagcagCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTT‑TTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACgtg                                                                                                               <  1:538850/141‑1 (MQ=255)
                           cagcGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACTTTATTT‑TTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTAt                                                                                                            >  2:280002/1‑141 (MQ=255)
                              cGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTT‑TTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAg                                                                                                         <  1:25668/141‑1 (MQ=255)
                                tttagttaTTTAATATTGCACGGTATTTTTAACGTCACATTATTT‑TTACATTCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCt                                                                                                       <  2:512854/134‑1 (MQ=255)
                                     tCATTTAATCTTGCACGGTATTTTTAAAGCAACCTTCTTT‑TTACACTCCGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGTCTGGATTGCCATGAGACGTGTATTAGCtcttat                                                                                                  >  2:235794/1‑141 (MQ=255)
                                       aTTTAATATTGCACGGTATTTTTAAAGCAACATTATTT‑TTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCg                                                                                                <  2:211566/141‑1 (MQ=255)
                                             tatTGCACGGTTTTTTTAAAGCGACGTTATTG‑TTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCAt                                                                                          <  2:347360/141‑1 (MQ=255)
                                              atTGCACGGTATTTTTAAAGCAACATTATTT‑TTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCAtt                                                                                         <  1:763403/141‑1 (MQ=255)
                                              atTGCACGGTATTTTTAAAGCAACATTATTT‑TTACAATCAGGTTCTATCCTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCAtt                                                                                         <  2:116330/141‑1 (MQ=255)
                                               ttGCACGGTATTTTTAAAGGGAC‑TGATTTGTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCAttt                                                                                        <  1:15099/141‑1 (MQ=255)
                                                tGCACGGTATTTTTATAGCAACATTATTT‑TTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTc                                                                                       >  1:575529/1‑141 (MQ=255)
                                                 gCACGGTATTTTTAAAGCAACATTATTT‑TTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCt                                                                                      >  1:377541/1‑141 (MQ=255)
                                                   atggTATTTTTAAAGCAACATTATTT‑TTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTAt                                                                                    <  1:705821/139‑1 (MQ=255)
                                                   aCGGTATTTTTAAAGCAACATTATTT‑TTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTAt                                                                                    <  1:519170/141‑1 (MQ=255)
                                                          ttttAGAGCAACATTATTT‑TTACAATCAGGTTCTATCGTGAAACGATTCAACATTTCAAGACAAGCATCTGCTAAAGAAGCTTATAGCTGGATTATCATGGGGAGTGTATTAGCGATTATCGTGGCATTGCTATTTTGGag                                                                             >  2:133756/1‑140 (MQ=255)
                                                          ttttAAAGCAACGTTATTT‑TTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGAt                                                                             <  1:21269/141‑1 (MQ=255)
                                                              aaaGCAACATTATTT‑TTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTCTTATCGTGGCATTTCTATTTTGGATGagt                                                                         >  2:253181/1‑141 (MQ=255)
                                                               aaGCAACACTATTT‑TTACAATCAGGTTCTATCGTGAAGCGCTTCAATATTCCAAAACAAGCATCTGATAAAGAAGCTTATGGCTGGATTGTCATGGGCCGTGTATTAGCTATTATCGTGGCATTTCTTTTTTGGATGagtg                                                                        >  2:328720/1‑140 (MQ=255)
                                                                   aaCATTATTT‑TTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGa                                                                    <  2:157777/141‑1 (MQ=255)
                                                                    aCATTATTT‑TTACAATCAGGTTCTATCGTGAAGCGATTCAATATGCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGAc                                                                   <  1:205795/141‑1 (MQ=255)
                                                                      attattT‑TTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAg                                                                 <  2:308917/141‑1 (MQ=255)
                                                                      attattT‑TTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAg                                                                 <  2:375672/141‑1 (MQ=255)
                                                                            t‑ttACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGc                                                           <  1:14857/141‑1 (MQ=255)
                                                                            t‑ttACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGc                                                           <  1:720829/141‑1 (MQ=255)
                                                                             ttgaCAATCCGGTTCTATCGGGAATCGATTGACTATTCCAATACAAGCAGCTGCTAAAGAAGCTTATGGCTGGACTGTCATGGGACGTGTAGTAGCTATTATCTTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGc                                                           <  1:724209/138‑1 (MQ=255)
                                                                                 cAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCatag                                                       >  1:196127/1‑140 (MQ=255)
                                                                                    tCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGACGTGCATATGaa                                                    >  1:588155/1‑141 (MQ=255)
                                                                                    tCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAACATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGaa                                                    >  2:509224/1‑141 (MQ=255)
                                                                                         ttCTATCGTGAAGCGATTCAATATTCCAAAACAAGCTTCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGtt                                               <  1:627676/141‑1 (MQ=255)
                                                                                         ttCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGCAGTGACAGATGCGCATATGATGTGtt                                               <  2:452340/141‑1 (MQ=255)
                                                                                          tCTATCGTGGAGCGATTCAATATTCCAAAACACGCATCTTCTAAAGAAGCTTATGGCTTGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTa                                              <  1:697337/141‑1 (MQ=255)
                                                                                          tCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTa                                              <  2:713276/141‑1 (MQ=255)
                                                                                            tATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAg                                            >  2:344305/1‑141 (MQ=255)
                                                                                                gTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCa                                        <  1:372691/141‑1 (MQ=255)
                                                                                                gTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCa                                        >  1:410490/1‑141 (MQ=255)
                                                                                                 tGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGCCGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCCTATGCAGTGTTAAGTGCAc                                       <  1:161890/141‑1 (MQ=255)
                                                                                                       gATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTc                                 <  2:43793/141‑1 (MQ=255)
                                                                                                       gATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTc                                 <  1:126982/141‑1 (MQ=255)
                                                                                                       gATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTc                                 <  1:638066/141‑1 (MQ=255)
                                                                                                          tCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAg                              <  1:173497/141‑1 (MQ=255)
                                                                                                           ccagaatCCAAAACAAGCATCTGCTAAAGCAGCCTACGGCTGGATTGTCATGGGACGTGGAGTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTCAAGTGCACTCATTCTAgg                             <  1:687409/135‑1 (MQ=255)
                                                                                                               atTCCAAAACCAGCATCTGTTAAATAAGCGTAAGGCTGGATTGTCATGGGACGTGTATTAGCTATTATAGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATgg                         <  1:158994/141‑1 (MQ=255)
                                                                                                                     aaaCAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTa                   >  2:146544/1‑141 (MQ=255)
                                                                                                                     aaaCAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTa                   >  2:146519/1‑141 (MQ=255)
                                                                                                                      aaCACGCATCTGCGAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTAc                  <  2:602779/141‑1 (MQ=255)
                                                                                                                      aaCAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTAATTAc                  >  1:228798/1‑141 (MQ=255)
                                                                                                                       aCAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTTCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACt                 <  1:267091/141‑1 (MQ=255)
                                                                                                                         aaGCATCTGCTAAAGAATCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTg               <  2:482662/141‑1 (MQ=255)
                                                                                                                          aGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGACGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGt              <  1:148266/141‑1 (MQ=255)
                                                                                                                           tcATCTGCTATAGACGCTGTTGGCTGGATTGTCATGGGACGTTTATTAGCTATTATCCTTGCATTTCTATTTTGTATTGGTTGTGGCAGTACTGCTGATGAGGTGTTAAGTGCATTCTTGCTAGGTTGGTCATTACTTgca             <  2:287085/140‑3 (MQ=255)
                                                                                                                           gCAGCTGCTAAAGAAGCTTATGGCTGGATTGTCGTTGTACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGAGGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTa             <  1:638997/141‑1 (MQ=255)
                                                                                                                             aTCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGGCATGACTTGTATc           >  1:121024/1‑141 (MQ=255)
                                                                                                                                tGCTAAAGAAGCTTATGGCTTGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCTTg        <  2:752552/141‑1 (MQ=255)
                                                                                                                                 gCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCTTgg       <  2:575529/141‑1 (MQ=255)
                                                                                                                                      aGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACGCATTCTAGGATGGTCATTACTTGTATCTTGGAATCa  <  2:286300/141‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                         
TTCAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTT‑TTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGACGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCTTGGAATCA  >  USA300TCH1516_ALE/478366‑478639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑