Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	788,134	A→T	5.8%	K100N (AAA→AAT)	USA300HOU_RS03860 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	788,134	0	A	T	5.8%	200.4 / 4.9	69	K100N (AAA→AAT)	USA300HOU_RS03860	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (26/38);  new base T (2/2);  total (29/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.51e-01

AATATAGACTTAAAATCGTATTAAATGCTGAAAGCAAATTTGAAACGATAGTGTCACCTTTACCTGAGAAAAGTAGTTTTACAGCAAAATTTCAAGTGTTGCCCAAAGTAGTTAATCCAACTTTTATAAAAAATAAAACGACAGAACGAAAGCATTTAGCACACAATCATGAAACAGATTTAATATTGCTAACTTCAGAGGACGGCAAGGTCCTTGAATTTGATATTGGCAACATTGTCATTGAAGAGGATGGAAAATGGTACACACCAAGTTATAA  >  USA300TCH1516_ALE/787998‑788274                                                                                                                                         |                                                                                                                                             aaTATAGACTTAAAATCGTATTAAATGCTGAAAGCAAATTTGAAACGATAGTGTCACCTTTACCTGAGAAAAGTAGTTTTACAGCAAAATTTCAAGTGTTGCCCAAAGTAGTTAATCCAACTTTAATAAAAAATAAAACGa                                                                                                                                          >  1:219455/1‑141 (MQ=255)  atatAGACTTAAAATCGTATTAAATGCTGAAAGCAAATTTGAAACGATAGTGTCACCTTTACCTGAGAAAAGTAGTTTTACAGCAAAATTTCAAGTGTTGCCCAAAGTAGTTAATCCAACTTTTATAAAAAATAAAACGAc                                                                                                                                         <  2:541647/141‑1 (MQ=255)   tataGACTTAAAATCGTATTAAATGCTGAAAGCAAATTTGAAACGATAGTGTCACCTTTACCTGAGAAAAGTAGTTTTACAGCAAAATTTCAAGTGTTGCCCAAAGTAGTTAATCCAACTTTTATAAAAAATAAAACGACa                                                                                                                                        <  2:565987/141‑1 (MQ=255)       gACTTAAAATCGTATTAAATGCTGAAAGCAAATTTGAAACGATAGTGTCACCTTTACCTGAGAAAAGTAGTTTTACAGCAAAATTTCAAGTGTTGCCCAAAGTAGTTAATCCAACTTTTATAAAAAATAAAACGACAGAAc                                                                                                                                    <  2:769233/141‑1 (MQ=255)        aCTTAAAATCGTATTAAATGCTGAAAGCAAATTTGAAACGATAGTGTCACCTTTACCTGAGAAAAGTAGTTTTACAGCAAAATTTCAAGTGTTGCCCAAAGTAGTTAATCCAACTTTTATAAAAAATAAAACGACAGAACg                                                                                                                                   <  1:64803/141‑1 (MQ=255)         cTTAAAATCGTATTAAATTCTGAAAGCAAATTTGAAACGATAGTGTCACCTTTACCTGAGAAAAGTAGTTTTACAGCAAAATTTCAAGTGTTGCCCAAAGTAGTTAATCCAACTTTTATAAAAAATAAAACGACAGAACGa                                                                                                                                  <  2:657626/141‑1 (MQ=255)             aaaTCGTATTAAATGCTGAAAGCAAATTTGAAACGATAGTGTCACCTTTACCTGAGAAAAGTAGTTTTACAGCAAAATTTCAAGTGTTGCCCAAAGTAGTTAATCCAACTTTTATAAAAAATAAAACGACAGAACGAAAGc                                                                                                                              >  1:665770/1‑141 (MQ=255)               aTCGTATTAAATGCTGAAAGCAAATTTGAAACGATAGTGTCACCTTTACCTGAGAAAAGTAGTTTTACAGCAAAATTTCAAGTGTTGCCCAAAGTAGTTAATCCAACTTTTATAAAAAATAAAACGACAGAACGAAAGCAt                                                                                                                            >  1:638644/1‑141 (MQ=255)               aTCGTATTAAATGCTGAAAGCAAATTTGAAACAATAGTGTCACCTTTACCTGAGAAAAGTAGTTTTACAGCAAAATTTCAAGTGATGCCCAAAGTAGTTAATCCAACTTTTATAAAAAATAAAACGACAGAACGAAAGCAt                                                                                                                            >  2:382753/1‑141 (MQ=255)                tCGTATTAAATGCTGAAAGCAAATTTGAAACGATAGTGTCACCTTTACCTGAGAAGAGTAGTTTTACAGCAAAATTTCAAGTGTTGCCCAACGTAGTTAATCCAACTTTGATAAAAAATAAAACGACAGAACGAAACCAtt                                                                                                                           >  2:240164/1‑141 (MQ=255)                tCGTATTAAATGCTGAAAGCAAATTTGAAACGATAGTGTCACCTTTACCTGAGAAAAGTAGTTTTACAGCAAAATTTCAAGTGTTGCCCAAAGTAGTTAATCCAACTTTTATAAAAAATAAAACGACAGAACGAAAGCAtt                                                                                                                           <  1:393899/141‑1 (MQ=255)                tCGTATTAAATGCTGAAAGCAAATTTGAAACGATAGTGTCACCTTTACCTGAGAAAAGTAGTTTTACAGCAAAATTTCAAGTGTTGCCCAAAGTAGTTAATCCAACTTTTATAAAAAATAAAACGACAGAACGAAAGCAtt                                                                                                                           <  1:623231/141‑1 (MQ=255)                 cGTATTAAATGCTGATAGCAAATTTGAAACGATAGTGTCACCTTTACCTGAGAAAAGTAGTTTTACAGCAAAATTTCAAGTGTTGCCCAAAGTAGTTAATCCAACTTTTATAAAAAATAATACGACAGAACGAAAGCAttt                                                                                                                          >  1:574118/1‑141 (MQ=255)                 cGTATTAAATGCTGAGAGCAAATTTGAAACGATAGTGTCACCTTTACCTGAGAAAAGTAGTTTTACAGCAAAATTTCAAGTGTTGCCCAAAGTAGTTAATCCAACTTTTATAAAAAATAATACGACAGAACGAAAGCAttt                                                                                                                          >  1:574124/1‑141 (MQ=255)                   tATTAAATGCTGAAAGCAAATTTGAAACGATAGTGTCACCTTTACCTGAGAAAAGTAGTTTTACAGCAAAATTTCAAGTGTTGCCCAAAGTAGTTAATCCAACTTTTATAAAAAATAAAACGACAGAACGAAAGCATTTAg                                                                                                                        <  1:87140/141‑1 (MQ=255)                            cTGAAAGCAAATTTGAAACGATAGTGTCACCTTTACCTGAGAAAAGTAGTTTTACAGCAAAATTTCAAGTGTTGCCCAAAGTAGTTAATCCAACTTTTATAAAAAATAAAACGACAGAACGAAAGCATTTAGCACACAATc                                                                                                               <  2:365006/141‑1 (MQ=255)                              gAAAGCAAATTTGAAACGATAGTGTCACCTTTACCTGAGAAAAGTAGTTTTACAGCAAAATTTCAAGTGTTGCCCAAAGTAGTTAATCCAACTTTTATAAAAAATAAAACGACAGAACGAAAGCATTTAGCACACAAtaaa                                                                                                             >  2:179807/1‑138 (MQ=255)                                aaGCAAATTTGAAACGATAGTGTCACCTTTACCTGAGAAAAGTAGTTTTACAGCAAAATTTCAAGTGTTGCCCAAAGTAGTTAATCCAACTTTTATAAAAAATAAAACGACAGAACGAAAGCATTTAGCACACAATCATGa                                                                                                           >  2:735199/1‑141 (MQ=255)                                aaGCAAATTTGAAACGATAGTGTCACCTTTACCTGAGAAAAGTAGTTTTACAGCAAAATTTCAAGTGTTGCCCAAAGTAGTTAATCCAACTTTTATAAAAAATAAAACGACAGAACGAAAGCATTTAGCACACAATCATGa                                                                                                           >  1:86884/1‑141 (MQ=255)                                aaGCAAATTTGAAACGATAGTGTCACCTTTACCTGAGAAAAGTAGTTTTACAGCAAAATTTCAAGTGTTGCCCAAAGTAGTTAATCCAACTTTTATAAAAAATAAAACGACAGAACGAAAGCATTTAGCAAACAATAATGa                                                                                                           >  2:630240/1‑141 (MQ=255)                                aaGCAAATTTGAAACGATAGGGTCACCTTTACCTGAGAAAAGTAATTTTACAGCAAAATTTCAAGTGTTGCCCAAAGTAGTTAATCCAACTTTTATAAAAAATAAAACGACAGAACGAAAGCATTTAGCACACAATCATGa                                                                                                           >  1:470400/1‑141 (MQ=255)                                    aaaTTTGAAACGATAGAGTCACCTGTACCTGAGAAAAGTAGTTTTACAGCAAAATTTCAAGTGTTGCCCAAAGTAGTTAAACCAACTTTTATAAAAAATAAAGCGACAGAACGAAAACATCACGCACACAATCATGAAACa                                                                                                       >  2:660842/1‑141 (MQ=255)                                      aTTTGAAACGATAGTGTCACCTTTACCTGAGAAAAGTAGTTTTACAGCAAAATTTCAAGTGTTGCCCAAAGTAGTTAATTCAACTTTTATAAAAAATAAAACGACAGAACGAAAGCATTTAGCACACAATCATGAAACAgt                                                                                                     >  2:13485/1‑140 (MQ=255)                                        ttGAAACGATAGTGTCACCTTTACCTGAGAAAAGTAGTTTTACAGCAAAATTTCAAGTGTTGCCCAAAGTAGTTAATCCAACTTTTATAAAAAATAAAACGACAGAACGAAAGCATTTAGCACACAATCATGAAACAGAtt                                                                                                   <  2:625901/141‑1 (MQ=255)                                           aaaCGATAGTGTCACCTTTACCTGAGAAAAGTAGTTTTACAGCAAAATTTCAAGTGTTGCCCAAAGTAGTTAATCCAACTTTTATAAAAAATAAAACGACAGAACGAAAGCATTTAGCACACAATCATGAAACAGATTTaa                                                                                                <  1:350688/141‑1 (MQ=255)                                                        aCCTTTACCTGAGAAAAGTAGTTTTACAGCAAAATTTCAAGTGTTGCCCAAAGTAGTTAATCCAACTTTTATAAAAAATAAAACGACAGAACGAAAGCATTTAGCACACAATCATGAAACAGATTTAATATTGCTAACTTc                                                                                   <  2:649788/141‑1 (MQ=255)                                                            ttACCTGAGAAAAGTAGTTTTACAGCAAAATTTCAAGTGTTGCCCAAAGTAGTTAATCCAACTTTTATAAAAAATAAAACGACAGAACGAAAGCATTTAGCACACAATCATGAAACAGATTTAATATTGCTAACTTCagag                                                                               >  2:390874/1‑141 (MQ=255)                                                            ttACCTGAGAAAAGTAGTTTTACAGCAAAATTTCAAGTGTTGCCCAAAGTAGTTAATCCAACTTTTATAAAAAATAAAACGACAGAACGAAAGCATTTAGCACACAATCAGGAAACAGATTTAATAGTGGTAACTTCagag                                                                               >  2:719712/1‑141 (MQ=255)                                                                cTGAGAAAAGTAGTTTTACAGCAAAATTTCAAGTGTTGCCCAAAGTAGTTAATCCAACTTTTATAAAAAATAAAACGACAGAACGAAAGCATTTAGCACACAATCATGAAACAGATTTAATATTGCTAACTTCAGAGGACg                                                                           <  1:291754/141‑1 (MQ=255)                                                                      aaaGTAGTTTTACACCAAAATTTCAAGTGTTGCCCAAAGTAGTTAATACAACTTTTATAAAAAATAAAACGACAGAACGAAAGCATTTAGCACACAATCATGAAACAGATTTAATATTGCTAACTTCAGAGGACGGCAAgg                                                                     <  2:754469/141‑1 (MQ=255)                                                                        agtagtTTTACAGCAAAATTTCAAGTGTTGCCCAAAGTAGTTAATCCAACTTTTATAAAAAATAAAACGACAGAACGAAAGCATTTAGCACACAATCATGAAACAGATTTAATATTGCTAACTTCCAAGGACGGCAAGGTc                                                                   >  1:233024/1‑141 (MQ=255)                                                                          tagtTTTACAGCAAAATTTCAAGTGTTGCCCAAAGTAGTTAATCCAACTTTTATAAAAAATAAAACGACAGAACGAAAGCATTTAGCACACAATCATGAAACAGATTTAATATTGCTAACTTCAGAGGACGGCAAGGTCCt                                                                 <  2:517956/141‑1 (MQ=255)                                                                            gtTTTACAGCAAAATTTCAAGTGTTGCCCAAAGTAGTTAATCCAACTTTTATAAAAAATAAAACGACAGAACGAAAGCATTTAGCACACAATCATGAAACAGATTTAATATTGCTAACTTCAGAGGACGGCAAGGTCCTTg                                                               <  1:124579/141‑1 (MQ=255)                                                                              tttACAGCAAAATTTCAAGTGTTGCCCAAAGTAGTTAATCCAACTTTTATAAAAAATAAAACGACAGAACGAAAGCATTTAGCACACAATCATGAAACAGATTTAATATTGCTAACTTCAGAGGACGGCAAGGTCCTTGaa                                                             >  1:741710/1‑141 (MQ=255)                                                                               ttACAGCAAAATTTCAAGTGTTGCCCAAAGTAGTTAATCCAACTTTTATAAAAAATAAAACGACAGAACGAAAGCATTTAGCACACAATCATGAAACAGATTTAATATTGCTAACTTCAGAGGACGGCAAGGTCCTTGAAt                                                            <  1:40351/141‑1 (MQ=255)                                                                               ttACAGCAAAATTTCAAGTGTTGCCCAAAGTAGTTAATCCAACTTTTATAAAAAATAAAACGACAGAACGAAAGCATTTAGCACACAATCATGAAACAGATTTAATATTGCTAACTTCAGAGGACGGCAAGGTCCTTGAAt                                                            <  1:714886/141‑1 (MQ=255)                                                                                 aCAGCAAAATTTCAAGTGTTGCCCAAAGTAGTTAATCCAACTTTTATAAAAAATAATACGACAGAACGAAAGCATTTAGCACACAATCATGAAACAGATTTAATATTGCTAACTTCAGAGGACGGCAAGGTCCTTGAAttt                                                          <  2:574118/141‑1 (MQ=255)                                                                                 aCAGCAAAATTTCAAGTGTTGCCCAAAGTAGTTAATCCAACTTTTATAAAAAATAATACGACAGAACGAAAGCATTTAGCACACAATCATGAAACAGATTTAATATTGCTAACTTCAGAGGACGGCAAGGTCCTTGAAttt                                                          <  2:574124/141‑1 (MQ=255)                                                                                          tttCAAGTGTTGCCCAAAGTAGTTAATCCAACTTTTATAAAAAATAAAACGACAGAACGAAAGCATTTAGCACACAATCATGAAACAGATTTAATATTGCTAACTTCAGAGGACGGCAAGGTCCTTGAATTTGATATTGGc                                                 <  1:658938/141‑1 (MQ=255)                                                                                          ttaaatgtgtTGCCAAAAGTAGTTACGCTAACTTGTAAGAAAAATATAACGACAGAACGAAAGCATTTAGCACACGTGCGTGAACCAGATTTTGCATTGCTAACTTCAGAGGACGGCAAGGTCCTTGGAGTTGATATTTGc                                                 <  2:163001/135‑1 (MQ=255)                                                                                             cAAGTGTTGCCCAACGTAGTTAATCCAACTTTTATAAAAAATAAAACGACAGAACGAAAGCATTTAGCACACAATCATAAAACAGATTTAATATTGCTACCTTCGGAGAACGGCAACGTACTTGAATTTGATATGCGCAAc                                              >  1:618930/1‑141 (MQ=255)                                                                                                gtgtTGCCCAAAGTAGTTAATCCAACTTTTATAAAAAATAAAACGACAGAACGAAAGCATTTAGCACACAATCATGAAACAGATTTAATATTGCTAACTTCAGAGGACGGCAAGGTCCTTGAATTTGATATTGGCAACAtt                                           >  2:71330/1‑141 (MQ=255)                                                                                                   ttGCCCAAAGTAGTTAATCCAACTTTTATAAAAAATAAAACGACAGAACGAAAGCATTTAGCACACAATCATGAAACAGATTTAATATTGCTAACTTCAGAGGACGGCAAGGTCCTTGAATTTGATATTGGCAACATTGTc                                        <  2:191777/141‑1 (MQ=255)                                                                                                   ttGCCAACAGTAGTTAATCCAACTTTTATAAAAAATAAAACGACAGAACGAAATCATTTAGCACACAATCATGAAACAGATTTAATATTGCTAACTTCAGAGTACGGCAAGGCCCTTGAATTTGATATTGGCAACATTGTc                                        <  2:129589/141‑1 (MQ=255)                                                                                                   tagCCCCAATTAGTTAAACCAATGTTGATAAACAATAAAACGACAGAACGATAGCATTCAGCACACAATCATGAAACAGATTTAATATTGCTAACTTCAGAGGACGGCAAGGTCCTTGAATTTGATATTGGCAACATTGTc                                        <  2:511520/139‑1 (MQ=255)                                                                                                      cccAAAGTAGTTAATCCAACTTTTATAAAAAATAAAACGACAGAACGAAAGCATTTAGCACACAATCATGAAACAGATTTAATATTGCTAACTTCAGAGGACGGCAAGGTCCTTGAATTTGATATTGGCAACATTGTCAtt                                     <  1:538425/141‑1 (MQ=255)                                                                                                            gtagtTAATCCAACTTTTATAAAAAATAAAACGACAGAACGAAAGCATTTAGCACACAATCATGAAACAGATTTAATATTGCTAACTTCAGAGGACGGCAAGGTCCTTGAATTTGATATTGGCAACATTGTCATTGAagag                               <  2:515188/141‑1 (MQ=255)                                                                                                              agtTGATCCAACTTTTATAAAAAATAAAACGGCAGAACGCAAGCATTTAGCACACAATCATGAAACAGATTTGATGTTGCTATCTTCAGAGGTCGGCAAGGTCCTTGAATTTGATATTGGCAACATTGTCATTGAAGAGGa                             <  2:713117/141‑1 (MQ=255)                                                                                                               gtTAATCCAACTTTTATAAAAAATAAAACGACAGAACGAAAGCATTTAGCACACAATCATGAAACAGATTTAATATTGCTAACTTCAGAGGACGGCAAGGTCCTTGAATTTGATATTGGCAACATTGTCATTGAAGAGGAt                            >  2:465540/1‑141 (MQ=255)                                                                                                               gtTAATCCAACTTTTATAAAAAATAAAACGAAAGAACGAAAGCATTTAGCACACAATCATGAAACAGATTTAATATTGCTAACTTCAGAGGACGGCAAGGTCCTTGAATTTGATATTGGCAACATTGTCATTGAAGAGGAt                            <  2:278140/141‑1 (MQ=255)                                                                                                               attAATCCAAGTTTTATAAAAAATACATCGCTGTAACGATAGCATTTATCACACATTCATGAAACAGATTTAATATTGCTAACTTCAGAGGACGGCAAGGTCCTTGAATTTGATATTGGCAACATTGTCATTGAAGAGGAt                            <  2:494817/140‑1 (MQ=255)                                                                                                               attAATCCAACTTTTATAAAAAATAAAACGACAGAACGAAAGCATTTAGCACACAATCATGAAACAGATTTAATATTGCTAACTTCAGAGGACGGCAAGGTCCTTGAATTTGATATTGGCAACATTGTCATTGAAGAGGAt                            <  2:465616/140‑1 (MQ=255)                                                                                                                 tAATCCAACTTTTATAAAAAATAAAACGACAGAACGAAAGCATTTAGCACACAATCATGAAACAGATTTAATATTGCTAACTTCAGAGGACGGCAAGGTCCTTGAATTTGATATTGGCAACATTGTCATTGAAGAGGATgg                          <  2:194810/141‑1 (MQ=255)                                                                                                                 tAATCCAACTTTTAAAAAAAATAAAACGACAGAACGAAAGCATTTAGCACACAATCATGAAACAGATTTAATATTGCTAACTTCAGAGGACGGCAAGGTCCTTGAATTTGATATTGGCAACATTGTCATTGAAGAGGATgg                          <  1:253539/141‑1 (MQ=255)                                                                                                                      cAACTTTTATAAAAAATAAAACGACAGAACGAAAGCATTTAGCACACAATCATGAAACAGATTTAATATTGCTAACTTCAGAGGACGGCAAGGTCCTTGAATTTGATATTGGCAACATTGTCATTGAAGAGGATGGAAAAt                     <  2:458218/141‑1 (MQ=255)                                                                                                                      cAACTTTTATAAAAAATAAAACGACAGAACGAAAGCATTTAGCACACAATCATGAAACAGATTTAATATTGCTAACTTCAGAGGACGGCAAGGTCCTTGAATTTGATATTGGCAACATTGTCATTGAAGAGGATGGAAAAt                     <  2:641642/141‑1 (MQ=255)                                                                                                                        aCTTTTATAAAAAATAAAGCGACAGAACGAAAGCATTTAGCACACAATCATGAAACAGATTTAATATTGCTAACTTCAGAAGCCGACAAGGTCCTTGAATTTGATATTGGCAAAATTGTCATTGAAGGGGAGGGAAAATgg                   >  2:10249/1‑141 (MQ=255)                                                                                                                         cTTTTATAAAAAATAAAACGACAGAACGAAAGCATTTAGCACACAATCATGAAACAGATTTAATATTGCTAACTTCAGAGGACGGCAAGGTCCTTGAATTTGATATTGGCAACATTGTCATTGAAGAGGATGGAAAATGGt                  <  1:477391/141‑1 (MQ=255)                                                                                                                           tttATAAAAAATAAAACGACAGAACGAAAGCATTTAGCACACAATCATGAAACAGATTTAATATTGCTAACTTCAGAGGACGGCAAGGACCTTGAATTTGATATTGGCAACATTGTCATTGAAGAGGATGGAAAATGGtca                >  2:677441/1‑139 (MQ=255)                                                                                                                           tttATAAAAAATAAAACGACAGAACGAAAGCATTTAGCACACAATCATGAAACAGATTTAATATTGCTAACTTCAGAGGACGGCAAGAATAACAAAGGTGATATTGGCAGCGTTGTCATTGAAGAGGATGGAAAATGGTac                >  1:559143/1‑141 (MQ=255)                                                                                                                            ttATAAAAAATAACACGACAGAACGAAAGCATTTCGCACACAATCATGAGACAAATTTAATGTTGCTAACTTCAGAGGACGGCAAGGGCATTGAATTTGATGTTGGCAACATTATCATTGAAGCGGAGGGAAAATGTTaca               >  2:301224/1‑141 (MQ=255)                                                                                                                             tataAAAAAAAAAACGACAGAACGAAAGCATTTAGCACACAATCATGAAACAGATTTAATATTGCTAACTTCAGAGGACGGCAAGGTCCTTGAATTTGATATTGGCAACATTGTCATTGAAGAGGATGGAAAATGGTacac              <  2:135294/141‑1 (MQ=255)                                                                                                                              ataAAAAATAAAACGACAGAACGAAAGCATTTAGCACACAATCATGAAACAGATTTAATATTGCTAACTTCAGAGGACGGCAAGGTCCTTGAATTTGATATTGGCAACATTGTCATTGAAGAGGATGGAAAATGGTacaca             <  2:392603/141‑1 (MQ=255)                                                                                                                               taAAAAATAAAACGACAGAACGAAAGCATTTAGCACACAATCATGAAACAGATTTAATATTGCTAACTTCAGAGGACGGCAAGGTCCTTGAATTTGATATTGGCAACATTGTCATTGGAGAGGATGGAAACTGGTacgaac            >  2:768722/1‑137 (MQ=255)                                                                                                                               taAAAAATAAAACGACAGAACGAAAGCATTTAGCACACAATCATGAAACAGATTTAATATTGCTAACTTCAGAGGACGGCAAGGTCCTTGAATTTGATATTGGCAACATTGTCATTGAAGAGGATGGAAAATGGTacacac            >  1:489037/1‑141 (MQ=255)                                                                                                                               taAAAAATAAAACGACAGAACGAAAGCATTTAGCACACAATCATGAAACAGATTTAATAATGCTAACTTCAGAAGACGGCAAGGTCCTTGAATTTGATATTGGCAACATTGTCATTGAAGAGGATGGAAAATGGTacacac            >  2:376855/1‑141 (MQ=255)                                                                                                                                aaaaaaaaaaACGACAGAACGAAAGCATTTAGCACACAATCAGAAAACAGATTTAATATTGCCAACTTCAGAGGACGGCAATTGCCTTGAATTTGAGATTGGCAACATTGTCATTGAAGAGTATGGTAGGTTTTACACAcc           <  2:242507/141‑1 (MQ=255)                                                                                                                                aaaaaaTAAAACGACAGAACGAAAGCATTTAGCACACAATCATGAAACAGATTTAATATTGCTAACTTCAGAGGACGGCAAGGTCCTTGAATTTGATATTGGCAACATTGTCATTGAAGAGGATGGAAAATGGTACACAcc           >  2:593722/1‑141 (MQ=255)                                                                                                                                   aaaTAAAACGACAGAACGAAAGCATTTAGCACACAATCATGAAACAGATTTAATATTGCTAACTTCAGAGGACGGCAAGGTCCTTGAATTTGATATTGGCAACATTGTCATTGAAGAGGATGGAAAATGGTACACACCAAg        <  1:609090/141‑1 (MQ=255)                                                                                                                                    aaTAAAACGACAGAACGAAAGCATTTAGCACACAATCAGGAAACAGATTTAATATTGCTAACTTCAGAGGACGGCAAGGTCCTTGAATTTGATATTGGCAACATTGTCATTGAAGAGGATGGAAAATGGTACACACCAAGt       <  2:226014/141‑1 (MQ=255)                                                                                                                                         aaCGACAGAACGAAAGCATTTAGCACACAATCATGAAACAGATTTAATATTGCTAACTTCAGAGGACGGCAAGGTCCTTGAATTTGATATTGGGAAAATTGTGATTGAAGAGGATGGAAAATGGTACACACCAAGTTATaa  >  2:160038/1‑141 (MQ=255)                                                                                                                                         aaCGACAGAACGAAAGCATTTAGCACACAATCATGAAACAGATTTAATATTGCTAACTTCAGAGGACGGCAAGGTCCTTGAATTTGATATTGGCAACATTTTCATTGAAGAGGATGGAAAATGGTACACACCAAGTTATaa  >  2:394238/1‑141 (MQ=255)                                                                                                                                         aaCGACAGAACGAAAGCATTTAGCACACAATCATGAAACAGATTTAATATTGCTAACTTCAGAGGACGGCAAGGTCCTTGAATTTGATATTGGCAACATTGTCATTGAAGAGGATGGAAAATGGTACACACCAAGTTATaa  <  1:699789/141‑1 (MQ=255)                                                                                                                                         |                                                                                                                                             AATATAGACTTAAAATCGTATTAAATGCTGAAAGCAAATTTGAAACGATAGTGTCACCTTTACCTGAGAAAAGTAGTTTTACAGCAAAATTTCAAGTGTTGCCCAAAGTAGTTAATCCAACTTTTATAAAAAATAAAACGACAGAACGAAAGCATTTAGCACACAATCATGAAACAGATTTAATATTGCTAACTTCAGAGGACGGCAAGGTCCTTGAATTTGATATTGGCAACATTGTCATTGAAGAGGATGGAAAATGGTACACACCAAGTTATAA  >  USA300TCH1516_ALE/787998‑788274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑