Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	1,105,994	T→C	100%	T12T (ACT→ACC)	pdhD →	Dihydrolipoyl dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,105,994	0	T	C	100.0%	234.6 / NA	66	T12T (ACT→ACC)	pdhD	Dihydrolipoyl dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/34);  total (32/34)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTGACCACAGACAAATTGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACTATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTCCTTCAA  >  USA300TCH1516_ALE/1105858‑1106124                                                                                                                                         |                                                                                                                                   ttGACCACAGACAAATTGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTCTTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAg                                                                                                                                >  2:582739/1‑141 (MQ=255)   gACCACAGACAAATTGATGGTGCAACTGGTCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTa                                                                                                                              <  2:660126/141‑1 (MQ=255)     ccACAGACAAATTGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACGGAGACCATAGTaaa                                                                                                                            >  2:310381/1‑140 (MQ=255)         aGACAAATTGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGa                                                                                                                        <  2:593698/141‑1 (MQ=255)           aCAAATTGATGGTGCAACTGGCCAAAATGCAATGAATCACATGAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGc                                                                                                                      >  2:582915/1‑141 (MQ=255)               aTTGATGGTGCAACTGGCCAAAGTGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGt                                                                                                                  >  2:564900/1‑141 (MQ=255)               aTTGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGt                                                                                                                  >  2:180018/1‑141 (MQ=255)               aTTGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGt                                                                                                                  >  2:596224/1‑141 (MQ=255)                  gATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGACTTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCt                                                                                                               <  1:417787/141‑1 (MQ=255)                  gATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCt                                                                                                               <  1:597399/141‑1 (MQ=255)                        gCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACACATCCCCTCGTAATCGGCGCCGGTCCtggtggc                                                                                                         >  1:661721/1‑140 (MQ=255)                        gCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTGCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGa                                                                                                         <  1:693821/141‑1 (MQ=255)                         cAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGAt                                                                                                        <  1:578326/141‑1 (MQ=255)                           aCTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCCGGTCCTGGTGGAtcc                                                                                                      >  2:421314/1‑139 (MQ=255)                                ccAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGGGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATAGGTTgc                                                                                                 >  1:130176/1‑141 (MQ=255)                                ccAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTgc                                                                                                 >  1:258537/1‑141 (MQ=255)                                ccAAAACGACATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTgc                                                                                                 >  2:358223/1‑141 (MQ=255)                                 cAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTgca                                                                                                >  1:517010/1‑141 (MQ=255)                                  aaaaTGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTgcag                                                                                               >  1:476792/1‑141 (MQ=255)                                    aaTGCAATGAATCACATTAAACGTTTATTAAAGAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTgcagca                                                                                             <  1:195294/141‑1 (MQ=255)                                        cAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTc                                                                                         <  1:281100/141‑1 (MQ=255)                                             aaTCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATGGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCa                                                                                    <  2:209948/141‑1 (MQ=255)                                             aaTCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCa                                                                                    <  2:256720/141‑1 (MQ=255)                                                cacaTTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCt                                                                                 <  1:474539/141‑1 (MQ=255)                                                  caTTAAACGTTTATTAAATAATCAAGAATTATTATTAATGGAGGGGTAAAGCATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTACTCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCa                                                                               >  1:80145/1‑141 (MQ=255)                                                      aaaCGTTTATTAAATAATCCAGAATTACTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTa                                                                           <  2:450057/141‑1 (MQ=255)                                                         cGTTTATTAAATAACCAAGGATAGTGATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAATCAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATAGGTTGAAGCAATTCGGGCAGCTCAATTAGGa                                                                        >  2:356468/1‑141 (MQ=255)                                                          gTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGAc                                                                       <  1:742269/141‑1 (MQ=255)                                                          gTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGAc                                                                       <  1:229305/141‑1 (MQ=255)                                                           tttATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACa                                                                      <  1:660365/141‑1 (MQ=255)                                                           tttATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTAGTGCAGGTAAATTAGGACa                                                                      >  1:137801/1‑141 (MQ=255)                                                            ttattaAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGCCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACaa                                                                     >  2:547519/1‑141 (MQ=255)                                                             tattaAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCACAATTGAAACATATACCAGAGTAATCGGAGCAGGTCATGGTGGATACGTTGAGGAAATCCGTGCGGCTCAATTAGGAGaaa                                                                    >  2:57853/1‑141 (MQ=255)                                                               ttaAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACaaaaa                                                                  >  1:717944/1‑141 (MQ=255)                                                               ttaAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTAGTGGAGCTCAGTTAGGACaaaaa                                                                  >  2:59458/1‑141 (MQ=255)                                                                tacataatCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGACATTACCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGTAATTCGTGCAGCTCAATTAGGACAAAAAg                                                                 <  2:191888/138‑1 (MQ=255)                                                                  aataatCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTa                                                               <  1:53218/141‑1 (MQ=255)                                                                    taatCCAGAATTATTATTAATGGGGGGGTCCACCATGGTTGTTGGAGATTTCCCAATTTAAACACATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAAc                                                             <  2:258537/141‑1 (MQ=255)                                                                    taatCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGTAGCTCAATTAGGACAAAAAGTAAc                                                             <  1:564900/141‑1 (MQ=255)                                                                    taatCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAAc                                                             <  1:180018/141‑1 (MQ=255)                                                                    taatCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAAc                                                             <  1:358223/141‑1 (MQ=255)                                                                        ccAGAATTATGATTAATGGAGGGGTAAAACAGGGTAGTTGGAGAGTGCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTAAAAAAAAAAAAAAAGTAACAATc                                                         <  2:145967/141‑1 (MQ=255)                                                                         cAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCg                                                        <  1:236907/141‑1 (MQ=255)                                                                           gAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATGCGTTGCAGCAATTCGTGCAGCCCAATTAGGACAAAAAGTAACAATCGtt                                                      >  1:6630/1‑141 (MQ=255)                                                                           gAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGtt                                                      >  1:78995/1‑141 (MQ=255)                                                                                attattaATGGAGGGGTAAAACAAGTTATTTGGATATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCCATTAGGACAAAAAGTAACAATCGTTGAGaa                                                 >  2:452390/1‑141 (MQ=255)                                                                                  tattaATGGAGGGGTAAAACATTGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTgggtagg                                               <  2:200100/141‑7 (MQ=255)                                                                                  tattaATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAg                                               <  2:200487/141‑1 (MQ=255)                                                                                    ttaATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGt                                             <  2:93578/141‑1 (MQ=255)                                                                                    ttaATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGt                                             <  1:32951/141‑1 (MQ=255)                                                                                    taaaTGGAGGGGTAAAACATGGTATTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGt                                             <  1:626436/139‑1 (MQ=255)                                                                                           aGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAAAAATCGTTGAGAAAGGTAATCTtg                                      >  2:520187/1‑141 (MQ=255)                                                                                            ggggTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTtgg                                     >  1:261745/1‑141 (MQ=255)                                                                                                      aTGGTAGTTGGAGATTCCCAAATTGAAACAGATACCATGGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGc                           >  1:431692/1‑141 (MQ=255)                                                                                                       tGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCt                          <  1:61073/141‑1 (MQ=255)                                                                                                                gagaTTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAg                 <  1:252735/141‑1 (MQ=255)                                                                                                                gagaTTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAg                 >  2:644116/1‑141 (MQ=255)                                                                                                                gagaTTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGGAATCTTGGTGGTGTTTGCTTAAACGTAg                 >  1:140292/1‑141 (MQ=255)                                                                                                                 agaTTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTGGGTGGTGTTTGCTTAAACGTAgg                >  2:114201/1‑141 (MQ=255)                                                                                                                     ttCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATATGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGt            >  1:99558/1‑141 (MQ=255)                                                                                                                     ttCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGt            >  2:754458/1‑141 (MQ=255)                                                                                                                       cccAATTGAAACAGATACCATGGTAATCGGAGCAGGTCCTGGTGGATACGGTGCAGCAATTCGTGCAGCTAAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTCTTTGCTTAAACGTAGGATGTAt          <  2:565128/141‑1 (MQ=255)                                                                                                                         cAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTc        >  2:246910/1‑141 (MQ=255)                                                                                                                          atttGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGTAGCGCAATTAGGACAAAAAGTAACAAGCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTcc       <  1:619399/139‑1 (MQ=255)                                                                                                                             tGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTCCTTc    <  1:679699/141‑1 (MQ=255)                                                                                                                               aaaCAGCTACCATAGGAACCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGGTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTATACGTCGGATGTATTCCTTCaa  <  1:398050/141‑1 (MQ=255)                                                                                                                                         |                                                                                                                                   TTGACCACAGACAAATTGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACTATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTCCTTCAA  >  USA300TCH1516_ALE/1105858‑1106124

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑