| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 1,196,508 | C→A | 100% | S338Y (TCT→TAT) | ftsZ → | Cell division protein FtsZ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 1,196,508 | 0 | C | A | 98.8% | 253.0 / ‑5.5 | 83 | S338Y (TCT→TAT) | ftsZ | Cell division protein FtsZ |
| Reads supporting (aligned to +/- strand): ref base C (2/0); new base A (32/48); total (34/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.69e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GATTTTCGGTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGA‑ACAAGCGTAAATACTTCTAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTAGAAATAGAGAAGAAAG > USA300TCH1516_ALE/1196371‑1196643 | gATTTTCGGTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGA‑ACAAGCGTAAATACTTATAg < 2:394756/141‑1 (MQ=255) ttttCGGTCCAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAATTATTGCAACTGGTTTTGATGACAAACCAACATCAGATGGTCGTAAATCTGGTAGCACTGGATTCGGA‑ACAAGCGTAAATACTTATAGCa > 2:569642/1‑141 (MQ=255) ttttCGGTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTTGAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGA‑ACAAGCGTAAATACTTATAGCa > 2:3657/1‑141 (MQ=255) ttttCGGTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGA‑ACAAGCGTAAATACTTATAgaa > 2:223089/1‑139 (MQ=255) ttttCGGTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGA‑ACAAGCGTAAATACTTATAGCa > 2:694621/1‑141 (MQ=255) ttttCGGTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGA‑ACAAGCGTAAATACTTATAGCa < 2:24574/141‑1 (MQ=255) ttttCGGTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGA‑ACAAGCGTAAATACTTATAGCa > 2:35220/1‑141 (MQ=255) tttCGGTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCCCTGGATTCGGA‑ACAAGCGTAAATACTTATAGCaa < 1:33016/141‑1 (MQ=255) cGGTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGA‑ACAAGCGTAAATACTTATAGCAATGc < 2:488857/141‑1 (MQ=255) gTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGA‑ACAAGCGTAAATACGTATAGCAATGGaa > 1:217721/1‑141 (MQ=255) gTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGA‑ACAAGCGTAAATAATTATAGCaacggtc > 2:574895/1‑136 (MQ=255) tACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGA‑ACAAGCGTAAATACTTATAGCAATGCAAc < 2:26841/141‑1 (MQ=255) ttattaATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGA‑ACAAGCGTAAATACTTATAGCAATGCAACGTCTa < 1:528074/141‑1 (MQ=255) attaATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGA‑AAAAGAGTAAAAACGTATAGCAATGCAAATTCTaaa > 2:45121/1‑141 (MQ=255) ttaATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGA‑ACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAg > 1:79864/1‑141 (MQ=255) ttaATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGA‑ACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAg > 1:745876/1‑141 (MQ=255) ttaATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGA‑ACAAGCGTAAATACTTATAGCAATGAAACTTCTAAAg > 1:745864/1‑141 (MQ=255) aTCCTGCATTACAAGATGAGATTATAGTAACAGATATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGA‑ACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATg > 2:452242/1‑141 (MQ=255) aTTACAAGATTAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATTACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGA‑ACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCAtt < 1:401911/141‑1 (MQ=255) aTTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGA‑ACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCAtt < 1:200047/141‑1 (MQ=255) ttACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGA‑ACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTc > 2:359011/1‑141 (MQ=255) ttACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGA‑ACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTc < 2:248450/141‑1 (MQ=255) aaGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGA‑ACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACtt < 1:747522/141‑1 (MQ=255) aaGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGA‑ACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACtt > 1:75789/1‑141 (MQ=255) caaTGAGATTGTTGTAACAGTCATTGCAACTTGTTTCGATGACAACCCAACATCACATGGTCGTACATCTGGTAGCACTTGATTCGGA‑ACAAGCGTACACACTTATAGCAATGCAACTTCTAAAGACGAATCATTCACTTc < 2:624854/139‑1 (MQ=255) aGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGTCAAACAACCATCACACGGTCGTAAATCTGGTAGCACTGGATTCGGA‑ACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTc < 1:213760/141‑1 (MQ=255) aGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGA‑ACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTc < 1:710020/141‑1 (MQ=255) aTTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGA‑ACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATtca > 1:776016/1‑141 (MQ=255) gttgtAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGA‑ACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGCTGACTCATTCACTACAAATtcatca > 2:697524/1‑141 (MQ=255) tAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGA‑ACAAGCGTAAATACTTATAGCAATGCAACTTCTAGAGTCGAATCATTCACTTCTGATTCATCAAATg < 2:130025/141‑1 (MQ=255) aaCAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGA‑ACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGc > 1:442632/1‑141 (MQ=255) aaCAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGA‑ACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGc > 1:442600/1‑141 (MQ=255) aGTTATTGCAACTGGTTTTGATTACAAACCAACATCACATGGTCGCAAATCTGGTAGCACTGGATTCGGA‑ACAAGCGTAAATACTTAGAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGcaca < 2:42591/141‑1 (MQ=255) aGTTATTGCAACTGGTTTTGATGACAAACCAATATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGA‑ACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGcaca < 1:344127/141‑1 (MQ=255) aGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGA‑ACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGcaca < 1:666243/141‑1 (MQ=255) aGTTATTGCAACTGGCTTTGATGACAAACCAACATCATATGGTCGTAAATCTGGTAGCACTGGATTCGGA‑ACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTTATCAAATTcaca < 1:546161/141‑1 (MQ=255) aGTTATTGCAACTGGATTTGATGACAAACCAACATCATATGGTCGTAAATCTGGTAGCACTGGATTCGGA‑ACAAGCGTAAATACTTATAGCAATCCAACTTCTAAAGATGAATCATTCACTTCAAATTTATCAAATTcaca < 1:546097/141‑1 (MQ=255) gTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAAACTGGTAGCACTGGACTCGGA‑ATAAGCGTAAATACTTCTGGCAATGCAACTTCTAAAAATCAATCAAGCTCTTCCAATTCTTCCAATGTACaa > 2:313842/1‑141 (MQ=255) ttATTGCCACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGA‑ACATGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAg < 2:26655/141‑1 (MQ=255) tATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGA‑ACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGc > 2:573660/1‑141 (MQ=255) aTTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGA‑ACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCa < 1:563439/141‑1 (MQ=255) ttGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGA‑ACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCaa < 1:450285/141‑1 (MQ=255) tGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGA‑ACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAAc < 1:654487/141‑1 (MQ=255) tGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGA‑ACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAAc < 1:704765/141‑1 (MQ=255) cAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGA‑ACACGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTg < 2:370532/141‑1 (MQ=255) cTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGA‑ACAAGCGTAAATACTTATCGCACTACAGCTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGTTa > 1:177489/1‑141 (MQ=255) ggtttGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGCCACGGGACT‑AGTACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAgtg < 1:367527/139‑1 (MQ=255) gTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGA‑ACAATCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCACATGCACAAGCAACTGATAgtg < 1:399160/141‑1 (MQ=255) ttGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGA‑ACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTaa < 1:649177/141‑1 (MQ=255) gatgaCAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGA‑ACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGt > 1:769950/1‑141 (MQ=255) atgaCAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGA‑ACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTg > 1:636924/1‑141 (MQ=255) atgaCAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGA‑ACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTg > 1:438992/1‑141 (MQ=255) gaCAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGCATTCGGA‑ACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTTTAAGTGaa > 2:508190/1‑141 (MQ=255) aCAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGA‑ACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGaaa > 1:2718/1‑141 (MQ=255) cAAACCAACATCACATGGTCGTAAATCTGGTAGCCCTGGATTCGGA‑ACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAg < 2:718907/141‑1 (MQ=255) cAAAACAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGA‑ACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATTAAATGCACAAGCAACTGATAGTGTAAGTGAAAg > 1:525677/1‑141 (MQ=255) aaCCAACATCACATGGTCCTAAATCTGGTAGCACTGGATTCGGA‑ACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGaa < 2:375361/141‑1 (MQ=255) ccAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGA‑ACAAGCGTAAATACTTCTAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAaca > 2:62313/1‑141 (MQ=255) cATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGA‑ACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATa < 1:45025/141‑1 (MQ=255) aaTCACAGGGTCATAAATCTGGTAGCACGGGATTCGTA‑ACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATa < 1:748714/140‑1 (MQ=255) tCACGTGGTCGTAAATATAGTAGCACTGGATTCGGA‑ACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGACGAAGCAAAGGTTAGTGTAAGTGAAAGAAAACATACa > 2:159174/1‑141 (MQ=255) tCACATGGTCGTAAATCTGGTAGCACTGGATTCGGA‑ACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGACAGAAAACATACa > 2:33633/1‑141 (MQ=255) tCACATGGTCGTAAATCTGGTAGCACTGGATTCGGA‑ACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACa < 1:213186/141‑1 (MQ=255) tCACATGATCGTAAATCTGGCAGCACTGGATTCGGA‑ACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACa > 1:485696/1‑141 (MQ=255) tGGTCGTAAATCTGGTAGGACTGGATTCGGA‑ACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTaa < 2:182508/141‑1 (MQ=255) gTCGTAAATCTGGTAGCACTGGATTCGGA‑ACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAaag < 1:306940/141‑1 (MQ=255) gTAAATCTGGTAGCACTGGATTCGGA‑ACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAaagaag < 1:515111/141‑1 (MQ=255) tAAATCTGGTAGCACTGGATTCGGA‑ACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAagaaga < 1:44529/141‑1 (MQ=255) aTCTGGTAGCACTGGATTCGGA‑ACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAgatga < 2:324969/141‑1 (MQ=255) aTCTGGTAGCACTGGATTCGGA‑ACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAgatga > 1:701647/1‑141 (MQ=255) gTAGCACTGGATTCGGA‑ACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTc < 1:723624/141‑1 (MQ=255) gTAGCACTGGATTCGGA‑ACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTc < 1:723636/141‑1 (MQ=255) tAGCACTGGATTCGGA‑ACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTcc < 1:604272/141‑1 (MQ=255) aGCACTGGATTCGGA‑ACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAACGAAAACATACAACTAAAGAAGATGATATGCCt > 1:330762/1‑141 (MQ=255) cTGGATTCGGA‑ACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAAATGATATTCCTAGCt < 1:573660/141‑1 (MQ=255) ggATTCGGA‑ACAAGCGTAAATACTTATAGCAATGCAACTTCTACAGATGAATCATTCACTTCAAACTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAACGAACCCATAAAACTAAAGCAGATGATATTCCTAGCTTc < 2:102138/141‑1 (MQ=255) aaGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTAGAAATag < 1:359011/141‑1 (MQ=255) gCGTAAATACTTTCAGCCATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTAGAAATagag < 2:558097/141‑1 (MQ=255) gCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTAGAAATagag < 2:776016/141‑1 (MQ=255) aaaTACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTAGAAATAGagaaga > 1:647641/1‑141 (MQ=255) tACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTAGAAATAGAGaagaaag < 1:379673/141‑1 (MQ=255) tACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTAGAAATAGAGaagaaag < 2:657084/141‑1 (MQ=255) aaCTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTAGAAATAGAGaagaaag < 2:660223/140‑1 (MQ=255) | GATTTTCGGTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGA‑ACAAGCGTAAATACTTCTAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTAGAAATAGAGAAGAAAG > USA300TCH1516_ALE/1196371‑1196643 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |